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The map label for this gene is lipB

Identifier: 145296169

GI number: 145296169

Start: 2305428

End: 2306183

Strand: Direct

Name: lipB

Synonym: cgR_2088

Alternate gene names: 145296169

Gene position: 2305428-2306183 (Clockwise)

Preceding gene: 145296168

Following gene: 145296170

Centisome position: 69.56

GC content: 53.17

Gene sequence:

>756_bases
ATGACTGCACCAAGAGATCCTTTTTTCCCCGCAGATCTTTCTATCCGCGCGTCTGCAGAGCCCATTGAAATTCAGCGGTT
GGGTTTGATCGATTATCAAGAGGCCTGGGATTATCAAGCAGAGCTTGCTACCCGTAGGGCTAATGATGAAATCCCTGATC
AGCTGCTTATTTTGGAGCACCCGTCGGTGTATACCGCAGGTAAGCGCACCCAGCCGGAAGATCTTCCCACCAACGGACTG
CCGGTGATCAATGCTGATCGTGGTGGTCGCATCACGTGGCATGGTCCTGGCCAATTGGTGATCTATCCGATTATCAAATT
AGCCGATCCGATCGATGTGGTTGATTACGTAAGACGCCTCGAGGAAGCGCTCATCCAAGTTGTCGGCGATATGGGTGTTG
CCGGCGCTGGGCGCATTGATGGGCGTTCGGGTGTGTGGGTGCCAGCTCATGATGGTTGGGTGGACAGCAAGGTTGCGGCC
ATCGGCATTCGAATAACTCGTGGTGTTGCAATGCACGGTGTGGCCATCAACTGCAACAACACGTTGGATTTCTATGAGCA
CATCATTCCGTGTGGCATTGCTGATGCAGGCTTGAGCACACTCTCGAGGGAACTGAAAAGGGACGTTTCAGTTGAGGAAT
TAGTCGAGCCATCGATCCGCGCATTGGATGATGCTTTGGCTGGTCGGCTGGTTGTTTCTGATCATTCTTTCGGCAGCGCG
CCCGACCCAACTAAGAATCTCCCTAAACGGGGGTAG

Upstream 100 bases:

>100_bases
AAACCGCCACTCCCCTTTCACTGGGGAGTGGCGGTTTTGTCGTTTCATGCATGCAGTGTGTGACTTATCAACCTTGTTAG
GGCTAGGGTGGATATCTATC

Downstream 100 bases:

>100_bases
TACGAGGAATTTTGTCGGTGGGGCGCCTCGTTGAAGCGAAGTAGAGCCGATTGCAGAATCGGCGGAATGAGACGTCGAAA
AGCGTTTAAGCTTTCCCTAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTAPRDPFFPADLSIRASAEPIEIQRLGLIDYQEAWDYQAELATRRANDEIPDQLLILEHPSVYTAGKRTQPEDLPTNGL
PVINADRGGRITWHGPGQLVIYPIIKLADPIDVVDYVRRLEEALIQVVGDMGVAGAGRIDGRSGVWVPAHDGWVDSKVAA
IGIRITRGVAMHGVAINCNNTLDFYEHIIPCGIADAGLSTLSRELKRDVSVEELVEPSIRALDDALAGRLVVSDHSFGSA
PDPTKNLPKRG

Sequences:

>Translated_251_residues
MTAPRDPFFPADLSIRASAEPIEIQRLGLIDYQEAWDYQAELATRRANDEIPDQLLILEHPSVYTAGKRTQPEDLPTNGL
PVINADRGGRITWHGPGQLVIYPIIKLADPIDVVDYVRRLEEALIQVVGDMGVAGAGRIDGRSGVWVPAHDGWVDSKVAA
IGIRITRGVAMHGVAINCNNTLDFYEHIIPCGIADAGLSTLSRELKRDVSVEELVEPSIRALDDALAGRLVVSDHSFGSA
PDPTKNLPKRG
>Mature_250_residues
TAPRDPFFPADLSIRASAEPIEIQRLGLIDYQEAWDYQAELATRRANDEIPDQLLILEHPSVYTAGKRTQPEDLPTNGLP
VINADRGGRITWHGPGQLVIYPIIKLADPIDVVDYVRRLEEALIQVVGDMGVAGAGRIDGRSGVWVPAHDGWVDSKVAAI
GIRITRGVAMHGVAINCNNTLDFYEHIIPCGIADAGLSTLSRELKRDVSVEELVEPSIRALDDALAGRLVVSDHSFGSAP
DPTKNLPKRG

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=229, Percent_Identity=33.6244541484716, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI87081767, Length=184, Percent_Identity=32.6086956521739, Blast_Score=101, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21355483, Length=222, Percent_Identity=36.4864864864865, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_CORGB (A4QFS2)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138990.1
- ProteinModelPortal:   A4QFS2
- SMR:   A4QFS2
- STRING:   A4QFS2
- GeneID:   4991951
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2088
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- ProtClustDB:   PRK14345
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 27323; Mature: 27192

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPRDPFFPADLSIRASAEPIEIQRLGLIDYQEAWDYQAELATRRANDEIPDQLLILEH
CCCCCCCCCCCCCEEECCCCCEEEEEECCEEHHHHHCHHHHHHHHHCCCCCCCEEEEEEC
PSVYTAGKRTQPEDLPTNGLPVINADRGGRITWHGPGQLVIYPIIKLADPIDVVDYVRRL
CCEEECCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEEHHHHCCCHHHHHHHHHH
EEALIQVVGDMGVAGAGRIDGRSGVWVPAHDGWVDSKVAAIGIRITRGVAMHGVAINCNN
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCEEEEEEEEEECC
TLDFYEHIIPCGIADAGLSTLSRELKRDVSVEELVEPSIRALDDALAGRLVVSDHSFGSA
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHCEEEEECCCCCCC
PDPTKNLPKRG
CCCCCCCCCCC
>Mature Secondary Structure 
TAPRDPFFPADLSIRASAEPIEIQRLGLIDYQEAWDYQAELATRRANDEIPDQLLILEH
CCCCCCCCCCCCEEECCCCCEEEEEECCEEHHHHHCHHHHHHHHHCCCCCCCEEEEEEC
PSVYTAGKRTQPEDLPTNGLPVINADRGGRITWHGPGQLVIYPIIKLADPIDVVDYVRRL
CCEEECCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEEEEHHHHCCCHHHHHHHHHH
EEALIQVVGDMGVAGAGRIDGRSGVWVPAHDGWVDSKVAAIGIRITRGVAMHGVAINCNN
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCEEEEEEEEEECC
TLDFYEHIIPCGIADAGLSTLSRELKRDVSVEELVEPSIRALDDALAGRLVVSDHSFGSA
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHCEEEEECCCCCCC
PDPTKNLPKRG
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA