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The map label for this gene is aceF [H]

Identifier: 145296168

GI number: 145296168

Start: 2303279

End: 2305312

Strand: Direct

Name: aceF [H]

Synonym: cgR_2087

Alternate gene names: 145296168

Gene position: 2303279-2305312 (Clockwise)

Preceding gene: 145296166

Following gene: 145296169

Centisome position: 69.5

GC content: 56.93

Gene sequence:

>2034_bases
ATGGCGTTCTCCGTAGAGATGCCCGAGCTGGGCGAATCAGTAACCGAAGGCACGATCACCCAGTGGTTGAAGTCTGTTGG
TGACACTGTTGAGGTAGATGAGCCGTTGCTCGAGGTCTCAACTGACAAGGTCGACACCGAGATTCCCTCTCCTGTCGCCG
GTGTCATCCTAGAGATTAAGGCTGAAGAGGATGACACCGTCGACGTCGGCGGGGTCATTGCAATAATCGGCGATGCTGAT
GAGACTCCTGCCAACGAAGCTCCTGCCGACGAGGCACCAGCACCTGCCGAAGAGGAAGAACCAGTTAAGGAAGAGCCAAA
GAAGGAGGCAGCTCCTGAAGCTCCAGCAGCAACTGGCGCAGCAACCGATGTGGAAATGCCAGAACTCGGCGAGTCCGTCA
CCGAAGGCACCATTACCCAGTGGCTCAAGGCTGTCGGCGACACCGTCGAAGTAGACGAACCACTTCTTGAGGTCTCCACC
GACAAGGTCGACACCGAAATCCCATCCCCAGTAGCAGGCACCATCGTGGAGATCCTTGCGGACGAAGACGACACCGTCGA
CGTCGGCGCAGTCATCGCCCGCATCGGTGACGCAAACGCAGCTGCAGCACCTGCCGAAGAGGAAGCAGCACCTGCCGAAG
AGGAAGCAGCACCTGCCGAAGAGGAAGAACCAGTTAAGGAAGAGCCAAAGAAGGAGGCAGCTCCTGAAGCTCCAGCAGCA
ACTGGCGCAGCAACCGATGTGGAAATGCCAGAACTCGGCGAATCCGTCACCGAAGGCACCATTACCCAGTGGCTCAAGGC
TGTCGGCGACACCGTCGAAGTAGACGAACCACTTCTTGAGGTCTCCACCGACAAGGTCGACACCGAAATCCCATCCCCAG
TAGCAGGCACCATCGTGGAGATCCTTGCAGACGAAGACGACACCGTCGACGTCGGCGCAGTCATCGCCCGCATCGGTGAC
GCAAACGCAGCTGCAGCACCTGCCGAAGAGGAAGCAGCTCCTGCCGAAGAGGAAGACCCAGTTAAGGAAGAGCCAAAGAA
GGAAGAGCCCAAGAAGGAAGCAGCTACTACACCTGCTGCGGCATCCGCAACTGTGTCCGCTTCTGGCGACAACGTTCCAT
ACGTCACCCCACTGGTGCGCAAGCTTGCTGAAAAGCACGGCGTTGACTTGAACACCGTGACCGGTACCGGTATCGGTGGC
CGTATCCGCAAGCAGGATGTTTTGGCTGCTGCGAACGACGAGGCTGCACCTGCTGAGGCTGCTGCTCCTGTTTCCGCTTG
GTCCACTAAGTCTGTTGATCCTGAGAAGGCTAAGCTCCGTGGTACCACTCAGAAGGTCAACCGCATCCGTGAGATCACCG
CGAAGAAGACCGTCGAGGCTCTGCAGATTTCTGCTCAGCTCACCCAGCTGCACGAGGTCGATATGACTCGCGTTGCTGAG
CTGCGTAAGAAGAACAAGCCCGCGTTCATCGAGAAGCACGGTGTGAACCTCACTTACCTGCCATTCTTCGTGAAGGCAGT
TGTCGAGGCTTTGGTTTCCCATCCAAACGTCAACGCGTCTTACAACGCGAAGACCAAGGAGATGACCTACCACTCCTCCG
TTAACCTCTCCATCGCTGTTGATACCCCAGCTGGTCTGTTGACCCCAGTCATTCACGATGCTCAGGATCTCTCCATCCCA
GAGATCGCAAAGGCAATTGTTGACCTGGCTGATCGTTCACGCAACAACAAGCTGAAGCCAAACGATCTGTCCGGTGGCAC
CTTCACCATCACCAACATTGGTTCTGAAGGCGCACTGTCTGATACCCCAATCCTGGTTCCGCCACAGGCTGGCATCTTGG
GCACCGGCGCGATCGTGAAGCGTCCAGTTGTCATCACCGAGGATGGAATTGATTCCATCGCGATCCGTCAGATGGTCTTC
CTGCCACTGACCTACGATCACCAGGTTGTAGATGGCGCAGATGCTGGTCGCTTCCTGACCACCATCAAGGACCGCCTTGA
GACCGCTAACTTCGAAGGCGATCTGCAGCTCTAA

Upstream 100 bases:

>100_bases
CTTCATATGACCCGAACACCACACATCACAAATTGAATCGGTATCCTTTGGGGTATTAGTTTCCGTTTTAACGACACGAC
TTGCGAGGAGTCTTAAAATA

Downstream 100 bases:

>100_bases
GATCTCTGGAAGTTAAAACCGCCACTCCCCTTTCACTGGGGAGTGGCGGTTTTGTCGTTTCATGCATGCAGTGTGTGACT
TATCAACCTTGTTAGGGCTA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MAFSVEMPELGESVTEGTITQWLKSVGDTVEVDEPLLEVSTDKVDTEIPSPVAGVILEIKAEEDDTVDVGGVIAIIGDAD
ETPANEAPADEAPAPAEEEEPVKEEPKKEAAPEAPAATGAATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVST
DKVDTEIPSPVAGTIVEILADEDDTVDVGAVIARIGDANAAAAPAEEEAAPAEEEAAPAEEEEPVKEEPKKEAAPEAPAA
TGAATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVSTDKVDTEIPSPVAGTIVEILADEDDTVDVGAVIARIGD
ANAAAAPAEEEAAPAEEEDPVKEEPKKEEPKKEAATTPAAASATVSASGDNVPYVTPLVRKLAEKHGVDLNTVTGTGIGG
RIRKQDVLAAANDEAAPAEAAAPVSAWSTKSVDPEKAKLRGTTQKVNRIREITAKKTVEALQISAQLTQLHEVDMTRVAE
LRKKNKPAFIEKHGVNLTYLPFFVKAVVEALVSHPNVNASYNAKTKEMTYHSSVNLSIAVDTPAGLLTPVIHDAQDLSIP
EIAKAIVDLADRSRNNKLKPNDLSGGTFTITNIGSEGALSDTPILVPPQAGILGTGAIVKRPVVITEDGIDSIAIRQMVF
LPLTYDHQVVDGADAGRFLTTIKDRLETANFEGDLQL

Sequences:

>Translated_677_residues
MAFSVEMPELGESVTEGTITQWLKSVGDTVEVDEPLLEVSTDKVDTEIPSPVAGVILEIKAEEDDTVDVGGVIAIIGDAD
ETPANEAPADEAPAPAEEEEPVKEEPKKEAAPEAPAATGAATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVST
DKVDTEIPSPVAGTIVEILADEDDTVDVGAVIARIGDANAAAAPAEEEAAPAEEEAAPAEEEEPVKEEPKKEAAPEAPAA
TGAATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVSTDKVDTEIPSPVAGTIVEILADEDDTVDVGAVIARIGD
ANAAAAPAEEEAAPAEEEDPVKEEPKKEEPKKEAATTPAAASATVSASGDNVPYVTPLVRKLAEKHGVDLNTVTGTGIGG
RIRKQDVLAAANDEAAPAEAAAPVSAWSTKSVDPEKAKLRGTTQKVNRIREITAKKTVEALQISAQLTQLHEVDMTRVAE
LRKKNKPAFIEKHGVNLTYLPFFVKAVVEALVSHPNVNASYNAKTKEMTYHSSVNLSIAVDTPAGLLTPVIHDAQDLSIP
EIAKAIVDLADRSRNNKLKPNDLSGGTFTITNIGSEGALSDTPILVPPQAGILGTGAIVKRPVVITEDGIDSIAIRQMVF
LPLTYDHQVVDGADAGRFLTTIKDRLETANFEGDLQL
>Mature_676_residues
AFSVEMPELGESVTEGTITQWLKSVGDTVEVDEPLLEVSTDKVDTEIPSPVAGVILEIKAEEDDTVDVGGVIAIIGDADE
TPANEAPADEAPAPAEEEEPVKEEPKKEAAPEAPAATGAATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVSTD
KVDTEIPSPVAGTIVEILADEDDTVDVGAVIARIGDANAAAAPAEEEAAPAEEEAAPAEEEEPVKEEPKKEAAPEAPAAT
GAATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVSTDKVDTEIPSPVAGTIVEILADEDDTVDVGAVIARIGDA
NAAAAPAEEEAAPAEEEDPVKEEPKKEEPKKEAATTPAAASATVSASGDNVPYVTPLVRKLAEKHGVDLNTVTGTGIGGR
IRKQDVLAAANDEAAPAEAAAPVSAWSTKSVDPEKAKLRGTTQKVNRIREITAKKTVEALQISAQLTQLHEVDMTRVAEL
RKKNKPAFIEKHGVNLTYLPFFVKAVVEALVSHPNVNASYNAKTKEMTYHSSVNLSIAVDTPAGLLTPVIHDAQDLSIPE
IAKAIVDLADRSRNNKLKPNDLSGGTFTITNIGSEGALSDTPILVPPQAGILGTGAIVKRPVVITEDGIDSIAIRQMVFL
PLTYDHQVVDGADAGRFLTTIKDRLETANFEGDLQL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=220, Percent_Identity=40.9090909090909, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI110671329, Length=447, Percent_Identity=29.082774049217, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI31711992, Length=565, Percent_Identity=28.3185840707965, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI203098816, Length=515, Percent_Identity=26.4077669902913, Blast_Score=137, Evalue=5e-32,
Organism=Homo sapiens, GI203098753, Length=469, Percent_Identity=26.865671641791, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=37.125748502994, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=37.0023419203747, Blast_Score=253, Evalue=4e-68,
Organism=Escherichia coli, GI1786305, Length=675, Percent_Identity=31.1111111111111, Blast_Score=225, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI25146366, Length=431, Percent_Identity=34.8027842227378, Blast_Score=196, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17537937, Length=442, Percent_Identity=30.7692307692308, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=29.5302013422819, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=317, Percent_Identity=28.391167192429, Blast_Score=100, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=444, Percent_Identity=31.981981981982, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=25.4464285714286, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=38.5650224215247, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=30.045871559633, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI20129315, Length=442, Percent_Identity=27.1493212669683, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24582497, Length=432, Percent_Identity=27.3148148148148, Blast_Score=113, Evalue=5e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 71048; Mature: 70917

Theoretical pI: Translated: 3.96; Mature: 3.96

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSVEMPELGESVTEGTITQWLKSVGDTVEVDEPLLEVSTDKVDTEIPSPVAGVILEIK
CCEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHCCCHHCCCCCCCCEEEEEEEE
AEEDDTVDVGGVIAIIGDADETPANEAPADEAPAPAEEEEPVKEEPKKEAAPEAPAATGA
CCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHCCCCCCCCCCC
ATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVSTDKVDTEIPSPVAGTIVEILA
CCCCCCHHHCCHHHHHHHHHHHHHHCCCEECCCHHHHHCCCHHCCCCCCCHHHHHHHHHC
DEDDTVDVGAVIARIGDANAAAAPAEEEAAPAEEEAAPAEEEEPVKEEPKKEAAPEAPAA
CCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCHHCCHHHCCCCCCCC
TGAATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVSTDKVDTEIPSPVAGTIVE
CCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCEECCCHHHHHCCCHHCCCCCCCHHHHHHH
ILADEDDTVDVGAVIARIGDANAAAAPAEEEAAPAEEEDPVKEEPKKEEPKKEAATTPAA
HHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
ASATVSASGDNVPYVTPLVRKLAEKHGVDLNTVTGTGIGGRIRKQDVLAAANDEAAPAEA
CCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHEECCCCCCCCHH
AAPVSAWSTKSVDPEKAKLRGTTQKVNRIREITAKKTVEALQISAQLTQLHEVDMTRVAE
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKKNKPAFIEKHGVNLTYLPFFVKAVVEALVSHPNVNASYNAKTKEMTYHSSVNLSIAV
HHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEE
DTPAGLLTPVIHDAQDLSIPEIAKAIVDLADRSRNNKLKPNDLSGGTFTITNIGSEGALS
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCC
DTPILVPPQAGILGTGAIVKRPVVITEDGIDSIAIRQMVFLPLTYDHQVVDGADAGRFLT
CCCEEECCCCCCEECCHHHHCCEEEECCCCCHHHHHHHHEEECCCCCCEECCCCCHHHHH
TIKDRLETANFEGDLQL
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AFSVEMPELGESVTEGTITQWLKSVGDTVEVDEPLLEVSTDKVDTEIPSPVAGVILEIK
CEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHCCCHHCCCCCCCCEEEEEEEE
AEEDDTVDVGGVIAIIGDADETPANEAPADEAPAPAEEEEPVKEEPKKEAAPEAPAATGA
CCCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHCCCCCCCCCCC
ATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVSTDKVDTEIPSPVAGTIVEILA
CCCCCCHHHCCHHHHHHHHHHHHHHCCCEECCCHHHHHCCCHHCCCCCCCHHHHHHHHHC
DEDDTVDVGAVIARIGDANAAAAPAEEEAAPAEEEAAPAEEEEPVKEEPKKEAAPEAPAA
CCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCHHCCHHHCCCCCCCC
TGAATDVEMPELGESVTEGTITQWLKAVGDTVEVDEPLLEVSTDKVDTEIPSPVAGTIVE
CCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCEECCCHHHHHCCCHHCCCCCCCHHHHHHH
ILADEDDTVDVGAVIARIGDANAAAAPAEEEAAPAEEEDPVKEEPKKEEPKKEAATTPAA
HHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
ASATVSASGDNVPYVTPLVRKLAEKHGVDLNTVTGTGIGGRIRKQDVLAAANDEAAPAEA
CCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHEECCCCCCCCHH
AAPVSAWSTKSVDPEKAKLRGTTQKVNRIREITAKKTVEALQISAQLTQLHEVDMTRVAE
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKKNKPAFIEKHGVNLTYLPFFVKAVVEALVSHPNVNASYNAKTKEMTYHSSVNLSIAV
HHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEE
DTPAGLLTPVIHDAQDLSIPEIAKAIVDLADRSRNNKLKPNDLSGGTFTITNIGSEGALS
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCC
DTPILVPPQAGILGTGAIVKRPVVITEDGIDSIAIRQMVFLPLTYDHQVVDGADAGRFLT
CCCEEECCCCCCEECCHHHHCCEEEECCCCCHHHHHHHHEEECCCCCCEECCCCCHHHHH
TIKDRLETANFEGDLQL
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]