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The map label for this gene is murE [H]

Identifier: 145296125

GI number: 145296125

Start: 2253512

End: 2255077

Strand: Reverse

Name: murE [H]

Synonym: cgR_2045

Alternate gene names: 145296125

Gene position: 2255077-2253512 (Counterclockwise)

Preceding gene: 145296126

Following gene: 145296124

Centisome position: 68.04

GC content: 54.79

Gene sequence:

>1566_bases
ATGGCAACCACGTTGCTGGACCTCACCAAACTTATCGATGGCATCCTCAAGGGCTCTGCCCAGGGCGTTCCCGCTCACGC
AGTAGGGGAACAAGCAATCGCGGCTATTGGTCTTGACTCCTCCAGCTTGCCTACCTCGGACGCTATTTTTGCTGCAGTTC
CAGGAACCCGCACTCACGGCGCACAGTTTGCAGGTACGGATAACGCTGCGAAAGCTGTGGCCATTTTGACTGACGCAGCT
GGACTTGAGGTGCTCAACGAAGCAGGAGAGACCCGCCCAGTCATCGTTGTTGATGATGTCCGCGCAGTACTTGGCGCAGC
ATCATCAAGCATTTATGGCGATCCTTCAAAAGATTTCACGCTCATTGGAGTCACTGGAACCTCAGGTAAAACCACCACCA
GCTACCTCTTGGAAAAAGGACTCATGGAGGCAGGGCACAAAGTTGGTTTGATCGGCACCACAGGTACACGTATTGACGGG
GAAGAAGTACCCACGAAGCTCACCACTCCAGAAGCGCCGACTCTGCAGGCATTGTTTGCTCGAATGCGCGATCACGGTGT
CACCCACGTGGTGATGGAAGTATCCAGCCATGCATTGTCATTGGGCAGGGTTGCGGGTTCCCACTTTGATGTAGCTGCGT
TTACCAACCTGTCGCAGGATCACCTTGATTTCCACCCCACCATGGATGATTACTTTGACGCGAAGGCATTGTTCTTCCGC
GCAGATTCTCCACTTGTGGCTGACAAACAGGTCGTGTGCGTGGATGATTCTTGGGGTCAGCGCATGGCCAGCGTGGCAGC
GGATGTGCAAACAGTATCCACCCTTGGGCAAGAAGCAGACTTCAGCGCTACAGACATCAATGTCAGCGACTCTGGCGCCC
AGAGTTTTAAGATCAACGCCCCCTCAAACCAGTCCTACCAGGTCGAGCTAGCTCTTCCAGGTGCGTTCAACGTTGCTAAC
GCCACGTTGGCATTTGCCGCTGCGGCACGCGTGGGTGTTGATGGCGAAGCGTTTGCTCGAGGCATGTCCAAGGTCGCGGT
TCCAGGCCGTATGGAACGCATTGATGAGGGACAAGACTTCCTTGCAGTGGTGGATTATGCCCACAAGCCTGCTGCAGTGG
CTGCTGTGTTGGATACGTTGAGGACCCAGATTGACGGGCGCCTCGGAGTGGTTATCGGTGCTGGTGGAGACCGCGATTCC
ACCAAGCGTGGCCCCATGGGGCAGTTGTCCGCACAGCGTGCTGATCTAGTTATTGTCACTGATGACAACCCTCGTTCAGA
GGTGCCTGCCACGATTCGCGCAGCAGTCACTGCAGGAGCACAGCAGGGTGCTTCAGAGTCCGAACGACCGGTGGAAGTCC
TAGAAATTGGTGACCGTGCAGAAGCAATTCGCGTTTTGGTCGAGTGGGCACAGCCTGGAGATGGCATTGTAGTAGCTGGA
AAAGGCCATGAAGTTGGACAACTAGTTGCTGGTGTCACCCACCATTTTGATGACCGCGAAGAAGTTCGCGCTGCTTTGAC
AGAAAAGCTCAACAATAAACTTCCCCTTACTACGGAAGAAGGATAG

Upstream 100 bases:

>100_bases
GAATTTCATAATCTGAACTTTTGCTTGAACTCTTTTCGGCATCACCCACGTGCCGCGTCCGAATTATTAACACCTAGAAA
CCTGTGGAGGAGAGAAAACC

Downstream 100 bases:

>100_bases
GCCACAGTCATGATCACAATGACCCTTGGGGAGATCGCTGACATCGTTGGAGGCAGGCTTACTGGCGGTGCTCAAGAAGA
TACGCTTGTGAGCTCCAGCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MATTLLDLTKLIDGILKGSAQGVPAHAVGEQAIAAIGLDSSSLPTSDAIFAAVPGTRTHGAQFAGTDNAAKAVAILTDAA
GLEVLNEAGETRPVIVVDDVRAVLGAASSSIYGDPSKDFTLIGVTGTSGKTTTSYLLEKGLMEAGHKVGLIGTTGTRIDG
EEVPTKLTTPEAPTLQALFARMRDHGVTHVVMEVSSHALSLGRVAGSHFDVAAFTNLSQDHLDFHPTMDDYFDAKALFFR
ADSPLVADKQVVCVDDSWGQRMASVAADVQTVSTLGQEADFSATDINVSDSGAQSFKINAPSNQSYQVELALPGAFNVAN
ATLAFAAAARVGVDGEAFARGMSKVAVPGRMERIDEGQDFLAVVDYAHKPAAVAAVLDTLRTQIDGRLGVVIGAGGDRDS
TKRGPMGQLSAQRADLVIVTDDNPRSEVPATIRAAVTAGAQQGASESERPVEVLEIGDRAEAIRVLVEWAQPGDGIVVAG
KGHEVGQLVAGVTHHFDDREEVRAALTEKLNNKLPLTTEEG

Sequences:

>Translated_521_residues
MATTLLDLTKLIDGILKGSAQGVPAHAVGEQAIAAIGLDSSSLPTSDAIFAAVPGTRTHGAQFAGTDNAAKAVAILTDAA
GLEVLNEAGETRPVIVVDDVRAVLGAASSSIYGDPSKDFTLIGVTGTSGKTTTSYLLEKGLMEAGHKVGLIGTTGTRIDG
EEVPTKLTTPEAPTLQALFARMRDHGVTHVVMEVSSHALSLGRVAGSHFDVAAFTNLSQDHLDFHPTMDDYFDAKALFFR
ADSPLVADKQVVCVDDSWGQRMASVAADVQTVSTLGQEADFSATDINVSDSGAQSFKINAPSNQSYQVELALPGAFNVAN
ATLAFAAAARVGVDGEAFARGMSKVAVPGRMERIDEGQDFLAVVDYAHKPAAVAAVLDTLRTQIDGRLGVVIGAGGDRDS
TKRGPMGQLSAQRADLVIVTDDNPRSEVPATIRAAVTAGAQQGASESERPVEVLEIGDRAEAIRVLVEWAQPGDGIVVAG
KGHEVGQLVAGVTHHFDDREEVRAALTEKLNNKLPLTTEEG
>Mature_520_residues
ATTLLDLTKLIDGILKGSAQGVPAHAVGEQAIAAIGLDSSSLPTSDAIFAAVPGTRTHGAQFAGTDNAAKAVAILTDAAG
LEVLNEAGETRPVIVVDDVRAVLGAASSSIYGDPSKDFTLIGVTGTSGKTTTSYLLEKGLMEAGHKVGLIGTTGTRIDGE
EVPTKLTTPEAPTLQALFARMRDHGVTHVVMEVSSHALSLGRVAGSHFDVAAFTNLSQDHLDFHPTMDDYFDAKALFFRA
DSPLVADKQVVCVDDSWGQRMASVAADVQTVSTLGQEADFSATDINVSDSGAQSFKINAPSNQSYQVELALPGAFNVANA
TLAFAAAARVGVDGEAFARGMSKVAVPGRMERIDEGQDFLAVVDYAHKPAAVAAVLDTLRTQIDGRLGVVIGAGGDRDST
KRGPMGQLSAQRADLVIVTDDNPRSEVPATIRAAVTAGAQQGASESERPVEVLEIGDRAEAIRVLVEWAQPGDGIVVAGK
GHEVGQLVAGVTHHFDDREEVRAALTEKLNNKLPLTTEEG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=483, Percent_Identity=35.1966873706004, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54249; Mature: 54118

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTLLDLTKLIDGILKGSAQGVPAHAVGEQAIAAIGLDSSSLPTSDAIFAAVPGTRTHG
CCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHEEEECCCCCCCCCCCCEEEECCCCCCCC
AQFAGTDNAAKAVAILTDAAGLEVLNEAGETRPVIVVDDVRAVLGAASSSIYGDPSKDFT
CEECCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCEE
LIGVTGTSGKTTTSYLLEKGLMEAGHKVGLIGTTGTRIDGEEVPTKLTTPEAPTLQALFA
EEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHH
RMRDHGVTHVVMEVSSHALSLGRVAGSHFDVAAFTNLSQDHLDFHPTMDDYFDAKALFFR
HHHHCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHCCCCHHCCCCCCCHHHHCCCEEEEEE
ADSPLVADKQVVCVDDSWGQRMASVAADVQTVSTLGQEADFSATDINVSDSGAQSFKINA
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEC
PSNQSYQVELALPGAFNVANATLAFAAAARVGVDGEAFARGMSKVAVPGRMERIDEGQDF
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCCCHHHHHCCCCCE
LAVVDYAHKPAAVAAVLDTLRTQIDGRLGVVIGAGGDRDSTKRGPMGQLSAQRADLVIVT
EEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEE
DDNPRSEVPATIRAAVTAGAQQGASESERPVEVLEIGDRAEAIRVLVEWAQPGDGIVVAG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEC
KGHEVGQLVAGVTHHFDDREEVRAALTEKLNNKLPLTTEEG
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCC
>Mature Secondary Structure 
ATTLLDLTKLIDGILKGSAQGVPAHAVGEQAIAAIGLDSSSLPTSDAIFAAVPGTRTHG
CCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHEEEECCCCCCCCCCCCEEEECCCCCCCC
AQFAGTDNAAKAVAILTDAAGLEVLNEAGETRPVIVVDDVRAVLGAASSSIYGDPSKDFT
CEECCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCEE
LIGVTGTSGKTTTSYLLEKGLMEAGHKVGLIGTTGTRIDGEEVPTKLTTPEAPTLQALFA
EEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHH
RMRDHGVTHVVMEVSSHALSLGRVAGSHFDVAAFTNLSQDHLDFHPTMDDYFDAKALFFR
HHHHCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHCCCCHHCCCCCCCHHHHCCCEEEEEE
ADSPLVADKQVVCVDDSWGQRMASVAADVQTVSTLGQEADFSATDINVSDSGAQSFKINA
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEC
PSNQSYQVELALPGAFNVANATLAFAAAARVGVDGEAFARGMSKVAVPGRMERIDEGQDF
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCCCHHHHHCCCCCE
LAVVDYAHKPAAVAAVLDTLRTQIDGRLGVVIGAGGDRDSTKRGPMGQLSAQRADLVIVT
EEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEE
DDNPRSEVPATIRAAVTAGAQQGASESERPVEVLEIGDRAEAIRVLVEWAQPGDGIVVAG
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEC
KGHEVGQLVAGVTHHFDDREEVRAALTEKLNNKLPLTTEEG
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]