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The map label for this gene is murF [H]

Identifier: 145296124

GI number: 145296124

Start: 2251958

End: 2253502

Strand: Reverse

Name: murF [H]

Synonym: cgR_2044

Alternate gene names: 145296124

Gene position: 2253502-2251958 (Counterclockwise)

Preceding gene: 145296125

Following gene: 145296123

Centisome position: 68.0

GC content: 55.15

Gene sequence:

>1545_bases
ATGATCACAATGACCCTTGGGGAGATCGCTGACATCGTTGGAGGCAGGCTTACTGGCGGTGCTCAAGAAGATACGCTTGT
GAGCTCCAGCGTGGAGTTTGATTCTCGATCCCTCACACCGGGTGGCTTGTTTTTAGCACTTCCGGGTGCTCGTGTAGACG
GCCATGATTTTGCTGCAACTGCAATTGAGAAAGGTGCGGTTGCAGTATTGGCAGCCCGTGAAGTTGACGTACCTGCGATC
GTCGTGCCTCCAGTAAAAATCCAGGAATCCAATGCTGATATTTATGCTCATGATCCAGATGGGCATGGCGCGGCGGTAGT
GGAGGCGTTGTCTCGGTTGGCTCGCCACGTGGTGGATATCTGCGTGGCTGGCCATCAATTGAACGTTGTGGCTATTACTG
GTTCTGCGGGAAAGACTTCTACGAAGGATTTCATCGCGACGGTTCTTGGCCAAGATGGGCCAACTGTGGCTCCTCCGGGC
TCGTTTAACAATGAGCTTGGTTTGCCACACACCGCGCTCCGCTGCACAACCGATACTAAGTATTTGGTGGCTGAGATGTC
CGCGCGTGGCATTGGACATATTAAGCACCTGACAGAGATTGCTCCGCCACGGATTGCAGCTGTGCTCAACGTCGGCCATG
CGCACCTGGGTGAATTTGGATCCCGCGAGAATATCGCGCAGGCAAAAGGCGAGATCATTGAAGCGCTGCCCTCGAAGAAA
ACGGGTGGGGTAGCAGTCCTTAACGCTGACGATCCTTTTGTCGCCCGGATGGCTCCACGCACTAAGGCGCGCGTGGTGTG
GTTTAGCACCGATGCGGGCCAAGCGAACAAGTCTGATTATTGGGCAACGAGTATTTCACTGGACGCTGTTGCGCGGGCAA
GCTTTACGCTGAACACGAAGGACGGCTCTTGGCCGGTCGCCCTGCAGGTTTTTGGTGAGCACCAGGTTGCTAATGCACTT
GCTGCTGCTGCCATTGCCATGGAAGCTGGCGTCGCCCCAGAATTGGTGGTTGCTGGATTGGAAGCACATTCAGCTGCTTC
CGCGCACCGCATGGATGTAAAGACCCGCGCCGACGGCGTGACCATCATCAACGATTCTTACAACGCGAATCCTGATTCTA
TGCGTGCAGGTATCGCGGCTCTTGCGTACACAGCTAGTGGTCGTTCTGAAGCAACAAGCTGGGCAGTGCTTGGCCAAATG
GGTGAGCTTGGCGATGACGCCTCGGAAGCCCATGCCGAACTTGGTGCTGAGCTGGCTAAATACAATGTTCAAGAACTTGT
CGCAGTGGGGGAGAACCCTAACTGTGCAGCACTTGCAGAGTCCGCAGCGAGCCTGGGTGTGAGTACTCACGTAGTTTCAG
ACGTTGATGCAGCGCTCGAGTTGCTCGCAGGCCATATTAAGCGGGATGATGTAGTGCTGGTTAAGGCTTCAAATGCTGAT
CGCCTGTGGAGGGTCGCAGAAGCACTACATGGCATGGTGCCGGGCCTCAAAAACACAGGTAGCTCGGTCAACGACGATTC
TCGTCGGAACGTGGAAGGACAGTAG

Upstream 100 bases:

>100_bases
CACCCACCATTTTGATGACCGCGAAGAAGTTCGCGCTGCTTTGACAGAAAAGCTCAACAATAAACTTCCCCTTACTACGG
AAGAAGGATAGGCCACAGTC

Downstream 100 bases:

>100_bases
AAAACAATGCAACAGATTATGGTCAGTGGAACGGTTGCGTTCCTCGTCTCAATCTTTCTCACCCCGGTGTTGATCCGTTA
TTTCACTAACCGCCAGTTGG

Product: hypothetical protein

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MITMTLGEIADIVGGRLTGGAQEDTLVSSSVEFDSRSLTPGGLFLALPGARVDGHDFAATAIEKGAVAVLAAREVDVPAI
VVPPVKIQESNADIYAHDPDGHGAAVVEALSRLARHVVDICVAGHQLNVVAITGSAGKTSTKDFIATVLGQDGPTVAPPG
SFNNELGLPHTALRCTTDTKYLVAEMSARGIGHIKHLTEIAPPRIAAVLNVGHAHLGEFGSRENIAQAKGEIIEALPSKK
TGGVAVLNADDPFVARMAPRTKARVVWFSTDAGQANKSDYWATSISLDAVARASFTLNTKDGSWPVALQVFGEHQVANAL
AAAAIAMEAGVAPELVVAGLEAHSAASAHRMDVKTRADGVTIINDSYNANPDSMRAGIAALAYTASGRSEATSWAVLGQM
GELGDDASEAHAELGAELAKYNVQELVAVGENPNCAALAESAASLGVSTHVVSDVDAALELLAGHIKRDDVVLVKASNAD
RLWRVAEALHGMVPGLKNTGSSVNDDSRRNVEGQ

Sequences:

>Translated_514_residues
MITMTLGEIADIVGGRLTGGAQEDTLVSSSVEFDSRSLTPGGLFLALPGARVDGHDFAATAIEKGAVAVLAAREVDVPAI
VVPPVKIQESNADIYAHDPDGHGAAVVEALSRLARHVVDICVAGHQLNVVAITGSAGKTSTKDFIATVLGQDGPTVAPPG
SFNNELGLPHTALRCTTDTKYLVAEMSARGIGHIKHLTEIAPPRIAAVLNVGHAHLGEFGSRENIAQAKGEIIEALPSKK
TGGVAVLNADDPFVARMAPRTKARVVWFSTDAGQANKSDYWATSISLDAVARASFTLNTKDGSWPVALQVFGEHQVANAL
AAAAIAMEAGVAPELVVAGLEAHSAASAHRMDVKTRADGVTIINDSYNANPDSMRAGIAALAYTASGRSEATSWAVLGQM
GELGDDASEAHAELGAELAKYNVQELVAVGENPNCAALAESAASLGVSTHVVSDVDAALELLAGHIKRDDVVLVKASNAD
RLWRVAEALHGMVPGLKNTGSSVNDDSRRNVEGQ
>Mature_514_residues
MITMTLGEIADIVGGRLTGGAQEDTLVSSSVEFDSRSLTPGGLFLALPGARVDGHDFAATAIEKGAVAVLAAREVDVPAI
VVPPVKIQESNADIYAHDPDGHGAAVVEALSRLARHVVDICVAGHQLNVVAITGSAGKTSTKDFIATVLGQDGPTVAPPG
SFNNELGLPHTALRCTTDTKYLVAEMSARGIGHIKHLTEIAPPRIAAVLNVGHAHLGEFGSRENIAQAKGEIIEALPSKK
TGGVAVLNADDPFVARMAPRTKARVVWFSTDAGQANKSDYWATSISLDAVARASFTLNTKDGSWPVALQVFGEHQVANAL
AAAAIAMEAGVAPELVVAGLEAHSAASAHRMDVKTRADGVTIINDSYNANPDSMRAGIAALAYTASGRSEATSWAVLGQM
GELGDDASEAHAELGAELAKYNVQELVAVGENPNCAALAESAASLGVSTHVVSDVDAALELLAGHIKRDDVVLVKASNAD
RLWRVAEALHGMVPGLKNTGSSVNDDSRRNVEGQ

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=495, Percent_Identity=35.1515151515151, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 53180; Mature: 53180

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMTLGEIADIVGGRLTGGAQEDTLVSSSVEFDSRSLTPGGLFLALPGARVDGHDFAAT
CEEEEHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHH
AIEKGAVAVLAAREVDVPAIVVPPVKIQESNADIYAHDPDGHGAAVVEALSRLARHVVDI
HHCCCCEEEEEEECCCCCEEEECCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
CVAGHQLNVVAITGSAGKTSTKDFIATVLGQDGPTVAPPGSFNNELGLPHTALRCTTDTK
HHCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
YLVAEMSARGIGHIKHLTEIAPPRIAAVLNVGHAHLGEFGSRENIAQAKGEIIEALPSKK
EEHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCC
TGGVAVLNADDPFVARMAPRTKARVVWFSTDAGQANKSDYWATSISLDAVARASFTLNTK
CCCEEEEECCCCEEECCCCCCCEEEEEEECCCCCCCCCCEEEEEEEHHHHEEEEEEEECC
DGSWPVALQVFGEHQVANALAAAAIAMEAGVAPELVVAGLEAHSAASAHRMDVKTRADGV
CCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHEECCHHHCCCCHHHEECEECCCCE
TIINDSYNANPDSMRAGIAALAYTASGRSEATSWAVLGQMGELGDDASEAHAELGAELAK
EEECCCCCCCHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YNVQELVAVGENPNCAALAESAASLGVSTHVVSDVDAALELLAGHIKRDDVVLVKASNAD
CCHHHHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHH
RLWRVAEALHGMVPGLKNTGSSVNDDSRRNVEGQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITMTLGEIADIVGGRLTGGAQEDTLVSSSVEFDSRSLTPGGLFLALPGARVDGHDFAAT
CEEEEHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHHH
AIEKGAVAVLAAREVDVPAIVVPPVKIQESNADIYAHDPDGHGAAVVEALSRLARHVVDI
HHCCCCEEEEEEECCCCCEEEECCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
CVAGHQLNVVAITGSAGKTSTKDFIATVLGQDGPTVAPPGSFNNELGLPHTALRCTTDTK
HHCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
YLVAEMSARGIGHIKHLTEIAPPRIAAVLNVGHAHLGEFGSRENIAQAKGEIIEALPSKK
EEHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCC
TGGVAVLNADDPFVARMAPRTKARVVWFSTDAGQANKSDYWATSISLDAVARASFTLNTK
CCCEEEEECCCCEEECCCCCCCEEEEEEECCCCCCCCCCEEEEEEEHHHHEEEEEEEECC
DGSWPVALQVFGEHQVANALAAAAIAMEAGVAPELVVAGLEAHSAASAHRMDVKTRADGV
CCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHEECCHHHCCCCHHHEECEECCCCE
TIINDSYNANPDSMRAGIAALAYTASGRSEATSWAVLGQMGELGDDASEAHAELGAELAK
EEECCCCCCCHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YNVQELVAVGENPNCAALAESAASLGVSTHVVSDVDAALELLAGHIKRDDVVLVKASNAD
CCHHHHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHH
RLWRVAEALHGMVPGLKNTGSSVNDDSRRNVEGQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]