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The map label for this gene is argG

Identifier: 145295535

GI number: 145295535

Start: 1616144

End: 1617349

Strand: Direct

Name: argG

Synonym: cgR_1463

Alternate gene names: 145295535

Gene position: 1616144-1617349 (Clockwise)

Preceding gene: 145295534

Following gene: 145295536

Centisome position: 48.76

GC content: 56.97

Gene sequence:

>1206_bases
ATGACTAACCGCATCGTTCTTGCATACTCCGGCGGTCTGGACACCACTGTGGCAATTCCATACCTGAAGAAGATGATTGA
TGGTGAAGTCATCGCAGTTTCCCTCGACCTGGGCCAGGGTGGAGAGAACCTGGACAACGTTCGCCAGCGTGCATTGGATG
CCGGTGCAGCTGAGTCCATCGTTGTGGATGCAAAGGATGAGTTCGCTGAGGAGTACTGCCTGCCAACCATCAAGGCAAAC
GGCATGTACATGAAGCAGTACCCACTGGTTTCTGCAATCTCCCGCCCACTGATCGTCAAGCACCTCGTTGAGGCTGGCAA
GCAGTTCAACGGTACCCACGTTGCACACGGCTGCACTGGTAAGGGCAACGACCAGGTTCGTTTCGAGGTCGGCTTCATGG
ACACCGATCCAAACCTGGAGATCATTGCACCTGCTCGTGACTTCGCATGGACCCGCGACAAGGCTATCGCCTTCGCCGAG
GAGAACAACGTTCCAATCGAGCAGTCCGTGAAGTCCCCATTCTCCATCGACCAGAACGTCTGGGGCCGCGCTATTGAGAC
CGGTTACCTGGAAGATCTGTGGAATGCTCCAACCAAGGACATCTACGCATACACCGAGGATCCAGCTCTGGGTAACGCTC
CAGATGAGGTCATCATCTCCTTCGAGGGTGGCAAGCCAGTCTCCATCGATGGCCGTCCAGTCTCCGTACTGCAGGCTATT
GAAGAGCTGAACCGTCGTGCAGGCGCACAGGGCGTTGGCCGCCTTGACATGGTTGAGGACCGTCTCGTGGGCATCAAGTC
CCGCGAAATCTACGAAGCACCAGGCGCAATCGCACTGATTAAGGCTCACGAGGCTTTGGAAGATGTCACCATCGAGCGCG
AACTGGCTCGCTACAAGCGCGGCGTTGACGCACGTTGGGCTGAGGAAGTATACGACGGCCTGTGGTTCGGACCTCTGAAG
CGCTCCCTGGACGCGTTCATTGATTCCACCCAGGAGCACGTCACCGGCGATATCCGCATGGTTCTGCACGCAGGTTCCAT
CACCATCAATGGTCGTCGTTCCAGCCACTCCCTGTACGACTTCAACCTGGCTACCTACGACACCGGCGACACCTTCGACC
AGACCCTGGCTAAGGGCTTTGTCCAGCTGCACGGTCTGTCCTCCAAGATCGCTAACAAGCGCGATCGCGAAGCTGGCAAC
AACTAA

Upstream 100 bases:

>100_bases
TTTTCGAGGAATACCAACCCTTTCAACACAATAATTTTCTTTAAACATCCTTGCTGTCCACCACGGCTGGCAAGGAACTT
AAAATGAAGGAGCACACCTC

Downstream 100 bases:

>100_bases
GCCACCTTTTCAAGCATCCAGACTAGAACTTCAAGTATTTAGAAAGTAGAAGAACAACACATGGAACAGCACGGAACCAA
TGAAGGTGCGCTGTGGGGCG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTNRIVLAYSGGLDTTVAIPYLKKMIDGEVIAVSLDLGQGGENLDNVRQRALDAGAAESIVVDAKDEFAEEYCLPTIKAN
GMYMKQYPLVSAISRPLIVKHLVEAGKQFNGTHVAHGCTGKGNDQVRFEVGFMDTDPNLEIIAPARDFAWTRDKAIAFAE
ENNVPIEQSVKSPFSIDQNVWGRAIETGYLEDLWNAPTKDIYAYTEDPALGNAPDEVIISFEGGKPVSIDGRPVSVLQAI
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAIALIKAHEALEDVTIERELARYKRGVDARWAEEVYDGLWFGPLK
RSLDAFIDSTQEHVTGDIRMVLHAGSITINGRRSSHSLYDFNLATYDTGDTFDQTLAKGFVQLHGLSSKIANKRDREAGN
N

Sequences:

>Translated_401_residues
MTNRIVLAYSGGLDTTVAIPYLKKMIDGEVIAVSLDLGQGGENLDNVRQRALDAGAAESIVVDAKDEFAEEYCLPTIKAN
GMYMKQYPLVSAISRPLIVKHLVEAGKQFNGTHVAHGCTGKGNDQVRFEVGFMDTDPNLEIIAPARDFAWTRDKAIAFAE
ENNVPIEQSVKSPFSIDQNVWGRAIETGYLEDLWNAPTKDIYAYTEDPALGNAPDEVIISFEGGKPVSIDGRPVSVLQAI
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAIALIKAHEALEDVTIERELARYKRGVDARWAEEVYDGLWFGPLK
RSLDAFIDSTQEHVTGDIRMVLHAGSITINGRRSSHSLYDFNLATYDTGDTFDQTLAKGFVQLHGLSSKIANKRDREAGN
N
>Mature_400_residues
TNRIVLAYSGGLDTTVAIPYLKKMIDGEVIAVSLDLGQGGENLDNVRQRALDAGAAESIVVDAKDEFAEEYCLPTIKANG
MYMKQYPLVSAISRPLIVKHLVEAGKQFNGTHVAHGCTGKGNDQVRFEVGFMDTDPNLEIIAPARDFAWTRDKAIAFAEE
NNVPIEQSVKSPFSIDQNVWGRAIETGYLEDLWNAPTKDIYAYTEDPALGNAPDEVIISFEGGKPVSIDGRPVSVLQAIE
ELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAIALIKAHEALEDVTIERELARYKRGVDARWAEEVYDGLWFGPLKR
SLDAFIDSTQEHVTGDIRMVLHAGSITINGRRSSHSLYDFNLATYDTGDTFDQTLAKGFVQLHGLSSKIANKRDREAGNN

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=396, Percent_Identity=36.1111111111111, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI16950633, Length=396, Percent_Identity=36.1111111111111, Blast_Score=238, Evalue=1e-62,
Organism=Escherichia coli, GI1789563, Length=398, Percent_Identity=29.3969849246231, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324514, Length=420, Percent_Identity=32.6190476190476, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI21358151, Length=400, Percent_Identity=34.5, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_CORGB (A4QDZ4)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138356.1
- ProteinModelPortal:   A4QDZ4
- SMR:   A4QDZ4
- STRING:   A4QDZ4
- GeneID:   4992939
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1463
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44117; Mature: 43986

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2; PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 87-87 BINDING 117-117 BINDING 119-119 BINDING 123-123 BINDING 123-123 BINDING 124-124 BINDING 127-127 BINDING 175-175 BINDING 259-259 BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRIVLAYSGGLDTTVAIPYLKKMIDGEVIAVSLDLGQGGENLDNVRQRALDAGAAESI
CCCEEEEEECCCCCCEEHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEE
VVDAKDEFAEEYCLPTIKANGMYMKQYPLVSAISRPLIVKHLVEAGKQFNGTHVAHGCTG
EEECHHHHHHHHCCCEEECCCEEEECCCHHHHHCCHHHHHHHHHCCCCCCCCEEECCCCC
KGNDQVRFEVGFMDTDPNLEIIAPARDFAWTRDKAIAFAEENNVPIEQSVKSPFSIDQNV
CCCCEEEEEEEEECCCCCEEEEECCHHCCCCCCCEEEEECCCCCCHHHHCCCCCCCCHHH
WGRAIETGYLEDLWNAPTKDIYAYTEDPALGNAPDEVIISFEGGKPVSIDGRPVSVLQAI
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHH
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAIALIKAHEALEDVTIERELARYKR
HHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
GVDARWAEEVYDGLWFGPLKRSLDAFIDSTQEHVTGDIRMVLHAGSITINGRRSSHSLYD
CCCCHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCEEEEEEECEEEECCCCCCCCEEE
FNLATYDTGDTFDQTLAKGFVQLHGLSSKIANKRDREAGNN
EEEEEECCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TNRIVLAYSGGLDTTVAIPYLKKMIDGEVIAVSLDLGQGGENLDNVRQRALDAGAAESI
CCEEEEEECCCCCCEEHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEE
VVDAKDEFAEEYCLPTIKANGMYMKQYPLVSAISRPLIVKHLVEAGKQFNGTHVAHGCTG
EEECHHHHHHHHCCCEEECCCEEEECCCHHHHHCCHHHHHHHHHCCCCCCCCEEECCCCC
KGNDQVRFEVGFMDTDPNLEIIAPARDFAWTRDKAIAFAEENNVPIEQSVKSPFSIDQNV
CCCCEEEEEEEEECCCCCEEEEECCHHCCCCCCCEEEEECCCCCCHHHHCCCCCCCCHHH
WGRAIETGYLEDLWNAPTKDIYAYTEDPALGNAPDEVIISFEGGKPVSIDGRPVSVLQAI
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHH
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAIALIKAHEALEDVTIERELARYKR
HHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
GVDARWAEEVYDGLWFGPLKRSLDAFIDSTQEHVTGDIRMVLHAGSITINGRRSSHSLYD
CCCCHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCEEEEEEECEEEECCCCCCCCEEE
FNLATYDTGDTFDQTLAKGFVQLHGLSSKIANKRDREAGNN
EEEEEECCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA