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The map label for this gene is pheS [H]

Identifier: 145295524

GI number: 145295524

Start: 1602942

End: 1604003

Strand: Direct

Name: pheS [H]

Synonym: cgR_1452

Alternate gene names: 145295524

Gene position: 1602942-1604003 (Clockwise)

Preceding gene: 145295523

Following gene: 145295525

Centisome position: 48.37

GC content: 57.34

Gene sequence:

>1062_bases
ATGAGGGAAGGGCTGGACTACACGGTGTCCGAAATTCAGTTGACCGAAGCCAGTTTGAACGAGGCGGCCGACGCGGCGAT
CAAGGCTTTCGACGGTGCACAAAACCTAGATGAACTCGCAGCACTACGCCGCGATCACTTGGGTGATGCCGCACCTATTC
CGCAGGCACGACGCTCACTGGGAACTATTCCTAAAGATCAGCGCAAGGATGCGGGCCGCTTTGTCAACATGGCACTTGGT
CGCGCAGAAAAGCACTTTGCTCAGGTCAAGGTTGTTTTGGAAGAAAAGCGCAACGCAGAAGTCCTAGAGCTTGAGCGCGT
TGACGTCACCGTTCCAACCACCCGCGAGCAGGTTGGTGCGCTGCACCCCATCACGATCCTCAACGAGCAGATCGCAGACA
TCTTCGTGGGCATGGGCTGGGAAATCGCCGAGGGACCAGAGGTGGAAGCAGAATACTTCAACTTCGACGCACTGAACTTC
CTGCCTGATCACCCAGCTCGTACACTGCAGGACACCTTCCACATTGCGCCGGAAGGCTCCCGCCAGGTGCTGCGTACCCA
CACCTCACCAGTTCAGGTGCGCACCATGCTCAACCGTGAAGTTCCGATCTACATCGCATGCCCTGGCCGTGTGTTCCGTA
CCGACGAACTCGATGCAACACACACCCCTGTGTTCCACCAGATCGAAGGCCTTGCAGTTGATAAGGGTCTGACTATGGCT
CACCTGCGCGGCACCTTGGATCACCTGGCCAAGGAACTCTTCGGCCCAGAGACCAAGACTCGTATGCGCTCCAACTACTT
CCCATTCACCGAGCCTTCCGCTGAGGTTGATGTGTGGTTCCCTAACAAGAAGGGTGGTGCAGGCTGGATCGAGTGGGGTG
GCTGTGGCATGGTCAACCCAAACGTGCTTCGTGCCGTGGGTGTTGACCCAGAGGAGTACACCGGATTCGCGTTCGGCATG
GGCATTGAACGTACCCTGCAGTTCCGCAACGGCCTGAGCGATATGCGCGACATGGTTGAGGGCGATATTCGCTTCACCCT
CCCATTCGGCATTCAGGCATAA

Upstream 100 bases:

>100_bases
TTTGTTTGTACGAATCCTCCAAGGCACTATTCGCCGGTGAGTAAACCCCAATTCATGCCCAGCGGGTATGATGGCGAGGT
TAGTTGACTTAACAATAAAC

Downstream 100 bases:

>100_bases
CTCATCCCGCACACGACTTATATATAGGAGCAAAATTCCACCATGTTGATTGCACAAAACTGGGTGACGGGACTGCTGGG
CCACGCAAACGAAGGCTGGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MREGLDYTVSEIQLTEASLNEAADAAIKAFDGAQNLDELAALRRDHLGDAAPIPQARRSLGTIPKDQRKDAGRFVNMALG
RAEKHFAQVKVVLEEKRNAEVLELERVDVTVPTTREQVGALHPITILNEQIADIFVGMGWEIAEGPEVEAEYFNFDALNF
LPDHPARTLQDTFHIAPEGSRQVLRTHTSPVQVRTMLNREVPIYIACPGRVFRTDELDATHTPVFHQIEGLAVDKGLTMA
HLRGTLDHLAKELFGPETKTRMRSNYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNPNVLRAVGVDPEEYTGFAFGM
GIERTLQFRNGLSDMRDMVEGDIRFTLPFGIQA

Sequences:

>Translated_353_residues
MREGLDYTVSEIQLTEASLNEAADAAIKAFDGAQNLDELAALRRDHLGDAAPIPQARRSLGTIPKDQRKDAGRFVNMALG
RAEKHFAQVKVVLEEKRNAEVLELERVDVTVPTTREQVGALHPITILNEQIADIFVGMGWEIAEGPEVEAEYFNFDALNF
LPDHPARTLQDTFHIAPEGSRQVLRTHTSPVQVRTMLNREVPIYIACPGRVFRTDELDATHTPVFHQIEGLAVDKGLTMA
HLRGTLDHLAKELFGPETKTRMRSNYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNPNVLRAVGVDPEEYTGFAFGM
GIERTLQFRNGLSDMRDMVEGDIRFTLPFGIQA
>Mature_353_residues
MREGLDYTVSEIQLTEASLNEAADAAIKAFDGAQNLDELAALRRDHLGDAAPIPQARRSLGTIPKDQRKDAGRFVNMALG
RAEKHFAQVKVVLEEKRNAEVLELERVDVTVPTTREQVGALHPITILNEQIADIFVGMGWEIAEGPEVEAEYFNFDALNF
LPDHPARTLQDTFHIAPEGSRQVLRTHTSPVQVRTMLNREVPIYIACPGRVFRTDELDATHTPVFHQIEGLAVDKGLTMA
HLRGTLDHLAKELFGPETKTRMRSNYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNPNVLRAVGVDPEEYTGFAFGM
GIERTLQFRNGLSDMRDMVEGDIRFTLPFGIQA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=273, Percent_Identity=30.4029304029304, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI5729820, Length=233, Percent_Identity=28.3261802575107, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1788007, Length=333, Percent_Identity=42.6426426426426, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI32563657, Length=273, Percent_Identity=31.1355311355311, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=273, Percent_Identity=31.1355311355311, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=281, Percent_Identity=29.1814946619217, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=264, Percent_Identity=30.3030303030303, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=174, Percent_Identity=28.735632183908, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI18858079, Length=271, Percent_Identity=33.210332103321, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137424, Length=287, Percent_Identity=27.5261324041812, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39351; Mature: 39351

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREGLDYTVSEIQLTEASLNEAADAAIKAFDGAQNLDELAALRRDHLGDAAPIPQARRSL
CCCCCCCCHHHEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
GTIPKDQRKDAGRFVNMALGRAEKHFAQVKVVLEEKRNAEVLELERVDVTVPTTREQVGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHCC
LHPITILNEQIADIFVGMGWEIAEGPEVEAEYFNFDALNFLPDHPARTLQDTFHIAPEGS
CCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHHEECCCCCC
RQVLRTHTSPVQVRTMLNREVPIYIACPGRVFRTDELDATHTPVFHQIEGLAVDKGLTMA
HHHHHHCCCCHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHCCEEECCCCHHH
HLRGTLDHLAKELFGPETKTRMRSNYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNP
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCC
NVLRAVGVDPEEYTGFAFGMGIERTLQFRNGLSDMRDMVEGDIRFTLPFGIQA
HHEEEECCCHHHHCCEEECCCHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCC
>Mature Secondary Structure
MREGLDYTVSEIQLTEASLNEAADAAIKAFDGAQNLDELAALRRDHLGDAAPIPQARRSL
CCCCCCCCHHHEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
GTIPKDQRKDAGRFVNMALGRAEKHFAQVKVVLEEKRNAEVLELERVDVTVPTTREQVGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHCC
LHPITILNEQIADIFVGMGWEIAEGPEVEAEYFNFDALNFLPDHPARTLQDTFHIAPEGS
CCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHHEECCCCCC
RQVLRTHTSPVQVRTMLNREVPIYIACPGRVFRTDELDATHTPVFHQIEGLAVDKGLTMA
HHHHHHCCCCHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCHHHHHCCEEECCCCHHH
HLRGTLDHLAKELFGPETKTRMRSNYFPFTEPSAEVDVWFPNKKGGAGWIEWGGCGMVNP
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCC
NVLRAVGVDPEEYTGFAFGMGIERTLQFRNGLSDMRDMVEGDIRFTLPFGIQA
HHEEEECCCHHHHCCEEECCCHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]