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The map label for this gene is leuC [H]

Identifier: 145295456

GI number: 145295456

Start: 1524126

End: 1525571

Strand: Direct

Name: leuC [H]

Synonym: cgR_1390

Alternate gene names: 145295456

Gene position: 1524126-1525571 (Clockwise)

Preceding gene: 145295454

Following gene: 145295457

Centisome position: 45.99

GC content: 59.31

Gene sequence:

>1446_bases
ATGACCAGCCCCGTGGAGAACAGCACCTCAACTGAGAAGCTGACCCTGGCAGAGAAGGTGTGGCGCGACCATGTCGTGTC
CAAGGGAGAAAACGGCGAGCCCGACCTCCTCTACATCGACCTGCAGCTGCTGCATGAAGTGACCTCACCACAGGCATTCG
ACGGCCTGCGCATGACTGGCCGCAAACTGCGCCACCCAGAACTGCACCTGGCCACCGAAGACCACAACGTGCCAACCGAA
GGCATCAAGACTGGCTCACTGCTGGAAATCAATGACCAGATTTCCCGCCTGCAGGTATCTACTCTGCGCGACAACTGTGA
AGAATTCGGCGTGCGACTGCACCCAATGGGCGACGTCCGACAGGGCATTGTGCACACTGTTGGCCCACAGCTGGGCGCAA
CCCAACCAGGCATGACCATTGTGTGCGGTGACTCCCACACCTCCANCCACGGCGCGTTTGGCTCCATGGCATTCGGTATC
GGTACCTCTGAGGTTGAGCACGTCATGGCCACTCAGACCCTGCCATTGAAGCCTTTCAAGACCATGGCCATTGAAGTTAC
TGGCGAACTGCAGCCAGGTGTTTCCTCCAAGGACCTGATTCTGGCGATTATTGCCAAGATCGGCACCGGCGGCGGACAGG
GCTACGTTCTGGAATACCGCGGCGAAGCAATCCGCAAGATGTCCATGGATGCACGCATGACCATGTGCAACATGTCCATC
GAAGCTGGCGCACGTGCCGGCATGATCGCCCCAGACCAAACCACCTTCGACTACGTTGAAGGCCGCGAAATGGCACCAAA
GGGCGCCGACTGGGACGAAGCAGTTGCTTACTGGAAGACCCTGCCAACCGACGAAGGCGCAACCTTTGACAAGGTCGTAG
AAATCGATGGCTCCGCACTGACCCCATTCATCACCTGGGGCACCAACCCAGGCCAGGGCCTGCCACTGGGCGAATCCGTA
CCAAGCCCAGAAGACTTCACCAACGACAACGACAAGGCAGCAGCCGAAAAGGCACTGCAGTACATGGACCTGGTACCAGG
AACCCCACTGCGCGACATCAAGATCGACACCGTCTTCCTGGGATCCTGTACCAACGCCCGCATCGAAGACCTGCAGATCG
CCGCTGATATCCTCAAGGGCCACAAAATCGCCGACGGCATGCGCATGATGGTCGTGCCTTCCTCCACCTGGATCAAGCAA
GAGGCCGAAGCGCTTGGGCTGGACAAAATCTTCACCGACGCTGGCGCTGAATGGCGTACCGCAGGCTGCTCCATGTGCCT
GGGCATGAACCCAGACCAACTGAAGCCAGGCGAGCGCTCTGCATCCACCTCCAACCGAAACTTCGAAGGACGCCAAGGAC
CAGGAGGCCGCACTCACCTGGTATCCCCAGCAGTCGCAGCCGCCACCGCAATCCGCGGCACCCTGTCCTCACCTGCAGAT
CTGTAA

Upstream 100 bases:

>100_bases
GAGGTGACATTTACGCCTCTTAATAGCTATACTGTCCCATGAAACGATATTTTAATCTCATATAGTGAGATTTACAGATT
TTTAAAGGACGGTGAGTTCC

Downstream 100 bases:

>100_bases
AAAGAAAGCTAGAAAACAATGGAGAAATTCACTACCCACACCGGCGTTGGCGTTCCACTGCAGCGATCCAACGTGGACAC
CGACCAGATCATCCCAGCCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTSPVENSTSTEKLTLAEKVWRDHVVSKGENGEPDLLYIDLQLLHEVTSPQAFDGLRMTGRKLRHPELHLATEDHNVPTE
GIKTGSLLEINDQISRLQVSTLRDNCEEFGVRLHPMGDVRQGIVHTVGPQLGATQPGMTIVCGDSHTSXHGAFGSMAFGI
GTSEVEHVMATQTLPLKPFKTMAIEVTGELQPGVSSKDLILAIIAKIGTGGGQGYVLEYRGEAIRKMSMDARMTMCNMSI
EAGARAGMIAPDQTTFDYVEGREMAPKGADWDEAVAYWKTLPTDEGATFDKVVEIDGSALTPFITWGTNPGQGLPLGESV
PSPEDFTNDNDKAAAEKALQYMDLVPGTPLRDIKIDTVFLGSCTNARIEDLQIAADILKGHKIADGMRMMVVPSSTWIKQ
EAEALGLDKIFTDAGAEWRTAGCSMCLGMNPDQLKPGERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAIRGTLSSPAD
L

Sequences:

>Translated_481_residues
MTSPVENSTSTEKLTLAEKVWRDHVVSKGENGEPDLLYIDLQLLHEVTSPQAFDGLRMTGRKLRHPELHLATEDHNVPTE
GIKTGSLLEINDQISRLQVSTLRDNCEEFGVRLHPMGDVRQGIVHTVGPQLGATQPGMTIVCGDSHTSXHGAFGSMAFGI
GTSEVEHVMATQTLPLKPFKTMAIEVTGELQPGVSSKDLILAIIAKIGTGGGQGYVLEYRGEAIRKMSMDARMTMCNMSI
EAGARAGMIAPDQTTFDYVEGREMAPKGADWDEAVAYWKTLPTDEGATFDKVVEIDGSALTPFITWGTNPGQGLPLGESV
PSPEDFTNDNDKAAAEKALQYMDLVPGTPLRDIKIDTVFLGSCTNARIEDLQIAADILKGHKIADGMRMMVVPSSTWIKQ
EAEALGLDKIFTDAGAEWRTAGCSMCLGMNPDQLKPGERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAIRGTLSSPAD
L
>Mature_480_residues
TSPVENSTSTEKLTLAEKVWRDHVVSKGENGEPDLLYIDLQLLHEVTSPQAFDGLRMTGRKLRHPELHLATEDHNVPTEG
IKTGSLLEINDQISRLQVSTLRDNCEEFGVRLHPMGDVRQGIVHTVGPQLGATQPGMTIVCGDSHTSXHGAFGSMAFGIG
TSEVEHVMATQTLPLKPFKTMAIEVTGELQPGVSSKDLILAIIAKIGTGGGQGYVLEYRGEAIRKMSMDARMTMCNMSIE
AGARAGMIAPDQTTFDYVEGREMAPKGADWDEAVAYWKTLPTDEGATFDKVVEIDGSALTPFITWGTNPGQGLPLGESVP
SPEDFTNDNDKAAAEKALQYMDLVPGTPLRDIKIDTVFLGSCTNARIEDLQIAADILKGHKIADGMRMMVVPSSTWIKQE
AEALGLDKIFTDAGAEWRTAGCSMCLGMNPDQLKPGERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAIRGTLSSPADL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=29.6703296703297, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI8659555, Length=449, Percent_Identity=23.6080178173719, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=23.0978260869565, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786259, Length=462, Percent_Identity=59.95670995671, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1787531, Length=365, Percent_Identity=26.027397260274, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081781, Length=359, Percent_Identity=25.6267409470752, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=366, Percent_Identity=31.1475409836066, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=366, Percent_Identity=31.1475409836066, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=320, Percent_Identity=31.25, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=407, Percent_Identity=24.3243243243243, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=452, Percent_Identity=60.8407079646018, Blast_Score=551, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6320440, Length=459, Percent_Identity=27.6688453159041, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=363, Percent_Identity=29.2011019283747, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=388, Percent_Identity=26.5463917525773, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281365315, Length=395, Percent_Identity=27.0886075949367, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17864292, Length=395, Percent_Identity=27.0886075949367, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161076999, Length=395, Percent_Identity=27.0886075949367, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=28.099173553719, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645686, Length=366, Percent_Identity=22.9508196721311, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=23.3062330623306, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51661; Mature: 51530

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPVENSTSTEKLTLAEKVWRDHVVSKGENGEPDLLYIDLQLLHEVTSPQAFDGLRMTG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHCCCCHHCCHHHCC
RKLRHPELHLATEDHNVPTEGIKTGSLLEINDQISRLQVSTLRDNCEEFGVRLHPMGDVR
HHCCCCCEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCEEECCCCHHH
QGIVHTVGPQLGATQPGMTIVCGDSHTSXHGAFGSMAFGIGTSEVEHVMATQTLPLKPFK
HHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHCCCCCCCCE
TMAIEVTGELQPGVSSKDLILAIIAKIGTGGGQGYVLEYRGEAIRKMSMDARMTMCNMSI
EEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHEEEEECCH
EAGARAGMIAPDQTTFDYVEGREMAPKGADWDEAVAYWKTLPTDEGATFDKVVEIDGSAL
HCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCEE
TPFITWGTNPGQGLPLGESVPSPEDFTNDNDKAAAEKALQYMDLVPGTPLRDIKIDTVFL
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
GSCTNARIEDLQIAADILKGHKIADGMRMMVVPSSTWIKQEAEALGLDKIFTDAGAEWRT
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHCHHHHHHCCCCCHHH
AGCSMCLGMNPDQLKPGERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAIRGTLSSPAD
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCCCC
L
C
>Mature Secondary Structure 
TSPVENSTSTEKLTLAEKVWRDHVVSKGENGEPDLLYIDLQLLHEVTSPQAFDGLRMTG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHCCCCHHCCHHHCC
RKLRHPELHLATEDHNVPTEGIKTGSLLEINDQISRLQVSTLRDNCEEFGVRLHPMGDVR
HHCCCCCEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCEEECCCCHHH
QGIVHTVGPQLGATQPGMTIVCGDSHTSXHGAFGSMAFGIGTSEVEHVMATQTLPLKPFK
HHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHCCCCCCCCE
TMAIEVTGELQPGVSSKDLILAIIAKIGTGGGQGYVLEYRGEAIRKMSMDARMTMCNMSI
EEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHEEEEECCH
EAGARAGMIAPDQTTFDYVEGREMAPKGADWDEAVAYWKTLPTDEGATFDKVVEIDGSAL
HCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCEE
TPFITWGTNPGQGLPLGESVPSPEDFTNDNDKAAAEKALQYMDLVPGTPLRDIKIDTVFL
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
GSCTNARIEDLQIAADILKGHKIADGMRMMVVPSSTWIKQEAEALGLDKIFTDAGAEWRT
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHCHHHHHHCCCCCHHH
AGCSMCLGMNPDQLKPGERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAIRGTLSSPAD
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]