Click here to switch to the map view.

The map label for this gene is leuD

Identifier: 145295457

GI number: 145295457

Start: 1525590

End: 1526183

Strand: Direct

Name: leuD

Synonym: cgR_1391

Alternate gene names: 145295457

Gene position: 1525590-1526183 (Clockwise)

Preceding gene: 145295456

Following gene: 145295460

Centisome position: 46.03

GC content: 56.73

Gene sequence:

>594_bases
ATGGAGAAATTCACTACCCACACCGGCGTTGGCGTTCCACTGCAGCGATCCAACGTGGACACCGACCAGATCATCCCAGC
CGTCTACCTCAAGCGCGTGACCCGCACCGGCTTCGAAGACGGACTGTTTTCCAACTGGCGCCAAAACGACCCCAACTTTG
TCCTCAACACTGAGACCTACAAAAACGGATCCGTTCTGATCGCTGGTCCTGACTTTGGCACCGGCTCCTCCCGCGAACAC
GCCGTCTGGGCACTCATGGACTACGGCTTCCGCGCTGTCTTCTCCTCCCGGTTCGCCGATATTTTCCGCGGCAACTCAGG
AAAAGCGGGCCTTTTGACCGGCATCATGGAACAGTCCGACATCGAACTTCTGTGGAAGCTCATGGAACAAACCCCGGGCC
TTGAACTGACTGTGAACCTGGAAAAGCAGATCGTCACCGCAGGCGACGTAGTGATCAGCTTCGAAGTTGACCCCTACATT
CGCTGGCGTTTGATGGAAGGCCTTGACGACGCTGGCCTGACCCTGCGCAAGCTCGATGAAATTGAAGACTACGAGGCTAA
GCGCCCTGCGTTCAAGCCACGCACTAACGCTTAA

Upstream 100 bases:

>100_bases
AGGCCGCACTCACCTGGTATCCCCAGCAGTCGCAGCCGCCACCGCAATCCGCGGCACCCTGTCCTCACCTGCAGATCTGT
AAAAAGAAAGCTAGAAAACA

Downstream 100 bases:

>100_bases
GTTTCAGTCTGATAGCGAAAGCACCCCGCAACCTTCATTGTCGCGGGGTGCATTTGTGCGTCTTGGTGGGCGAGCGGAGT
GGGCATGTCTGGAATAGACC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MEKFTTHTGVGVPLQRSNVDTDQIIPAVYLKRVTRTGFEDGLFSNWRQNDPNFVLNTETYKNGSVLIAGPDFGTGSSREH
AVWALMDYGFRAVFSSRFADIFRGNSGKAGLLTGIMEQSDIELLWKLMEQTPGLELTVNLEKQIVTAGDVVISFEVDPYI
RWRLMEGLDDAGLTLRKLDEIEDYEAKRPAFKPRTNA

Sequences:

>Translated_197_residues
MEKFTTHTGVGVPLQRSNVDTDQIIPAVYLKRVTRTGFEDGLFSNWRQNDPNFVLNTETYKNGSVLIAGPDFGTGSSREH
AVWALMDYGFRAVFSSRFADIFRGNSGKAGLLTGIMEQSDIELLWKLMEQTPGLELTVNLEKQIVTAGDVVISFEVDPYI
RWRLMEGLDDAGLTLRKLDEIEDYEAKRPAFKPRTNA
>Mature_197_residues
MEKFTTHTGVGVPLQRSNVDTDQIIPAVYLKRVTRTGFEDGLFSNWRQNDPNFVLNTETYKNGSVLIAGPDFGTGSSREH
AVWALMDYGFRAVFSSRFADIFRGNSGKAGLLTGIMEQSDIELLWKLMEQTPGLELTVNLEKQIVTAGDVVISFEVDPYI
RWRLMEGLDDAGLTLRKLDEIEDYEAKRPAFKPRTNA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=48.7309644670051, Blast_Score=189, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321429, Length=201, Percent_Identity=40.7960199004975, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_CORGB (A4QDS9)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138278.1
- ProteinModelPortal:   A4QDS9
- SMR:   A4QDS9
- STRING:   A4QDS9
- GeneID:   4992999
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1391
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22183; Mature: 22183

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTTHTGVGVPLQRSNVDTDQIIPAVYLKRVTRTGFEDGLFSNWRQNDPNFVLNTETY
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEECCCC
KNGSVLIAGPDFGTGSSREHAVWALMDYGFRAVFSSRFADIFRGNSGKAGLLTGIMEQSD
CCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCH
IELLWKLMEQTPGLELTVNLEKQIVTAGDVVISFEVDPYIRWRLMEGLDDAGLTLRKLDE
HHHHHHHHHHCCCCEEEEECCCEEEEECCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHH
IEDYEAKRPAFKPRTNA
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEKFTTHTGVGVPLQRSNVDTDQIIPAVYLKRVTRTGFEDGLFSNWRQNDPNFVLNTETY
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEECCCC
KNGSVLIAGPDFGTGSSREHAVWALMDYGFRAVFSSRFADIFRGNSGKAGLLTGIMEQSD
CCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCH
IELLWKLMEQTPGLELTVNLEKQIVTAGDVVISFEVDPYIRWRLMEGLDDAGLTLRKLDE
HHHHHHHHHHCCCCEEEEECCCEEEEECCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHH
IEDYEAKRPAFKPRTNA
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA