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The map label for this gene is ispH

Identifier: 145295167

GI number: 145295167

Start: 1219839

End: 1220816

Strand: Direct

Name: ispH

Synonym: cgR_1109

Alternate gene names: 145295167

Gene position: 1219839-1220816 (Clockwise)

Preceding gene: 145295164

Following gene: 145295173

Centisome position: 36.81

GC content: 56.44

Gene sequence:

>978_bases
ATGAGCTCACCTGTTATCAGCCCCGAAACCAAAACCGGAAAGAAGATCCTGCTTGCAGCCCCTCGCGGATACTGCGCCGG
CGTAGACCGTGCAGTGGAAACCGTCGAGCGCGCGCTCGAGGAATACGGCGCCCCAATTTATGTCCGTAAAGAAATCGTGC
ACAACCGTTACGTTGTGGACACCCTGGCAGAAAAGGGCGCGATTTTTGTCAACGAAGCATCTGAAGCACCAGAAGGTGCC
AACATGGTGTTCTCTGCACACGGCGTGAGCCCAATGGTCCACGAAGAGGCCGCAGCTAAAAACATCAAGGCTATTGACGC
GGCCTGCCCGCTGGTCACCAAAGTGCACAAGGAAGTCCAGCGCTTTGATAAGCAGGGATTCCACATTCTCTTCATCGGTC
ACGAAGGCCATGAAGAAGTAGAGGGCACCATGGGTCATTCCGTTGAGAAAACCCACCTGGTTGACGGCGTTGCTGGCATT
GCCACCCTGCCTGAATTCTTAAACGATGAACCAAACCTGATCTGGCTGTCTCAGACCACGCTGTCTGTGGACGAGACCAT
GGAGATCGTCCGCGAGCTGAAGGTGAAGTTCCCTCAGCTGCAGGATCCACCGTCAGATGATATTTGCTACGCCACGCAGA
ACCGCCAGGTTGCCGTCAAGGCTATCGCTGAGCGCTGCGAGCTGATGATTGTGGTCGGTTCCCGCAACTCCTCCAACTCG
GTTCGTCTGGTTGAGGTCGCTAAGCAAAACGGTGCCGATAACGCCTACCTGGTGGATTACGCCCGCGAAATCGACCCAGC
ATGGTTCGAAGGCGTAGAGACCATCGGTATCTCCTCCGGCGCTTCCGTGCCTGAGATCCTCGTCCAGGGCGTCATTGAGC
GCCTGGCTGAGTTCGGCTACGACGACGTCGAGGAAGTCACCTCCGCGGCTGAGAAGATTGTTTTCGCGCTGCCTCGCGTG
CTGCGCCACAAGAATTAA

Upstream 100 bases:

>100_bases
TCCTTCTTCCACCAGCTAAAAGTGAATTACCCCGCCTTTGTCGGGGTGGTTGCATTCCCAGTCTAGGTGTTTAGCCTCAA
CGTTGGATACGCTGGGAGGC

Downstream 100 bases:

>100_bases
TTGCCAGAATGAAAAATCCCCGCCACATGAGCGGGGATTTTTTAGCTGTACAGATCATCGTCGAGCGAGATGCGGCGTTT
AGGGGCTGCTTGTCGACGCT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSSPVISPETKTGKKILLAAPRGYCAGVDRAVETVERALEEYGAPIYVRKEIVHNRYVVDTLAEKGAIFVNEASEAPEGA
NMVFSAHGVSPMVHEEAAAKNIKAIDAACPLVTKVHKEVQRFDKQGFHILFIGHEGHEEVEGTMGHSVEKTHLVDGVAGI
ATLPEFLNDEPNLIWLSQTTLSVDETMEIVRELKVKFPQLQDPPSDDICYATQNRQVAVKAIAERCELMIVVGSRNSSNS
VRLVEVAKQNGADNAYLVDYAREIDPAWFEGVETIGISSGASVPEILVQGVIERLAEFGYDDVEEVTSAAEKIVFALPRV
LRHKN

Sequences:

>Translated_325_residues
MSSPVISPETKTGKKILLAAPRGYCAGVDRAVETVERALEEYGAPIYVRKEIVHNRYVVDTLAEKGAIFVNEASEAPEGA
NMVFSAHGVSPMVHEEAAAKNIKAIDAACPLVTKVHKEVQRFDKQGFHILFIGHEGHEEVEGTMGHSVEKTHLVDGVAGI
ATLPEFLNDEPNLIWLSQTTLSVDETMEIVRELKVKFPQLQDPPSDDICYATQNRQVAVKAIAERCELMIVVGSRNSSNS
VRLVEVAKQNGADNAYLVDYAREIDPAWFEGVETIGISSGASVPEILVQGVIERLAEFGYDDVEEVTSAAEKIVFALPRV
LRHKN
>Mature_324_residues
SSPVISPETKTGKKILLAAPRGYCAGVDRAVETVERALEEYGAPIYVRKEIVHNRYVVDTLAEKGAIFVNEASEAPEGAN
MVFSAHGVSPMVHEEAAAKNIKAIDAACPLVTKVHKEVQRFDKQGFHILFIGHEGHEEVEGTMGHSVEKTHLVDGVAGIA
TLPEFLNDEPNLIWLSQTTLSVDETMEIVRELKVKFPQLQDPPSDDICYATQNRQVAVKAIAERCELMIVVGSRNSSNSV
RLVEVAKQNGADNAYLVDYAREIDPAWFEGVETIGISSGASVPEILVQGVIERLAEFGYDDVEEVTSAAEKIVFALPRVL
RHKN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=311, Percent_Identity=50.1607717041801, Blast_Score=309, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CORGB (A4QCY9)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137988.1
- STRING:   A4QCY9
- GeneID:   4991701
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1109
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 35603; Mature: 35472

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPVISPETKTGKKILLAAPRGYCAGVDRAVETVERALEEYGAPIYVRKEIVHNRYVVD
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHHHH
TLAEKGAIFVNEASEAPEGANMVFSAHGVSPMVHEEAAAKNIKAIDAACPLVTKVHKEVQ
HHHHCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFDKQGFHILFIGHEGHEEVEGTMGHSVEKTHLVDGVAGIATLPEFLNDEPNLIWLSQTT
HHHHCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
LSVDETMEIVRELKVKFPQLQDPPSDDICYATQNRQVAVKAIAERCELMIVVGSRNSSNS
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCEEEEEEECCCCCCC
VRLVEVAKQNGADNAYLVDYAREIDPAWFEGVETIGISSGASVPEILVQGVIERLAEFGY
EEEEEEEHHCCCCCCEEEHHHHHCCHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
DDVEEVTSAAEKIVFALPRVLRHKN
CCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSPVISPETKTGKKILLAAPRGYCAGVDRAVETVERALEEYGAPIYVRKEIVHNRYVVD
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHHHH
TLAEKGAIFVNEASEAPEGANMVFSAHGVSPMVHEEAAAKNIKAIDAACPLVTKVHKEVQ
HHHHCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFDKQGFHILFIGHEGHEEVEGTMGHSVEKTHLVDGVAGIATLPEFLNDEPNLIWLSQTT
HHHHCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
LSVDETMEIVRELKVKFPQLQDPPSDDICYATQNRQVAVKAIAERCELMIVVGSRNSSNS
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCEEEEEEECCCCCCC
VRLVEVAKQNGADNAYLVDYAREIDPAWFEGVETIGISSGASVPEILVQGVIERLAEFGY
EEEEEEEHHCCCCCCEEEHHHHHCCHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
DDVEEVTSAAEKIVFALPRVLRHKN
CCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA