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The map label for this gene is prfC [H]

Identifier: 145295087

GI number: 145295087

Start: 1138064

End: 1139812

Strand: Reverse

Name: prfC [H]

Synonym: cgR_1030

Alternate gene names: 145295087

Gene position: 1139812-1138064 (Counterclockwise)

Preceding gene: 145295093

Following gene: 145295085

Centisome position: 34.39

GC content: 61.52

Gene sequence:

>1749_bases
GTGGCAAAAAACGTCGACAAGCAGGCAACGCCGGCGTGCAACTTCGCTGAGCTGGGGCGACTATGGCCCAGCGCCCACAA
CCCGCTATTCTTAATACCCATGAGCAACGCCAATCCCGACACCACCGCCGCCGAGGCACATCGCCGCAGAACATTCGCCG
TAATCGCACACCCCGACGCCGGTAAATCCACCCTCACCGAGGCATTGGCGCTGCATGCACACATCATCTCCGAAGCCGGC
GCCACCCACGGCAAAGCAGGCCGCAAAGCCACCGTTTCCGACTGGATGGAAATGGAAAAAGACCGCGGCATCTCCATCGC
CTCCTCCGCACTCCAATTCGAGTACGCACCAGAAGGCCACGAAGGCGAACCCTTCATGATCAACCTCGTGGATACCCCAG
GCCACGCCGACTTCTCCGAAGACACCTACCGCGTCCTCATGGCCGTCGACGCCGCAGTCATGCTTATCGACGCCGCCAAA
GGCCTCGAACCCCAAACCCTCCAACTCTTCCGCGTCTGCAAAGCCCGCGGCCTGCCAATCATCACCGTGATCAACAAATG
GGACCGCGTCGGCCGCACCCCACTCGAGCTCGTCGACGAAATCGTCAAAGAAATCGAACTCCAACCGACCCCACTGTTCT
GGCCAGTTGGTGAAGCCGGCGACTTCCGCGGCCTAGCCCGCATCAACAACGACGGCGAAGCCGAAGAATACATCCACTTC
ACCCGCACCGCCGGCGGCTCCACCATTGCCCCCGAAGACTTCTACACCCCAGAAGAAGCCATCGAACGCGAAGGCGACGT
CTGGGAAAACGCCACCGAAGAAGCAGAACTCCTCGCGGCCGACGGCGCCGTCCACGACCAGGAACTCTTCCTCAACTGCA
CCACCTCCCCACTGATCTTCGCCTCCGCGATGCTCAACTTCGGCGTCCACCAAATCCTGGACACCCTCTGCCAACTCGCA
CCATCCCCCGCCGGCCGCGACGCAGACCCCAAAGCCCTCGAAGCCGCCACCTCCGCAATGGACGACCACCGCGACACCAC
CGACGACTTCTCCGGCGTCGTCTTCAAAGTCCAAGCCGGCATGGACAAAAACCACCGCGATACCCTCGCCTTCATGCGCG
TCGTCTCCGGCGAATTCGACCGCGGCATGCAAGTCACCCACTCCCAATCCGGCCGCAGCTTCTCCACCAAATACGCCCTC
ACCGTCTTCGGCCGCACCCGCTCCACCGTCGAAACCGCCTTCCCCGGCGACATCGTCGGCCTCGTCAACGCTGGCGCCCT
CGCACCAGGCGACACCATCTTCGAAGGCCGAAAAATCCAATACCCACCAATGCCAAAATTCGCGCCAGAACACTTCCGCA
TCCTGCGCGCCAAATCACTCGGCAAATACAAACAGTTCCGCAAAGCCCTCGAGCAGCTGGACTCCGAAGGTGTCGTCCAG
ATCCTCAAGAACGACCTGCGTGGCGACGCCAACCCAGTCATGGCCGCTGTCGGCCCAATGCAGTTCGAAGTGATGCAAGC
CCGCATGGAAGTCGAATACAACGTCGAAACCGTCGCCGATCCGATTCCATACTCCGTCGCCCGCCGCACCACCGCCGAAA
CCGCACCAGAACTGGCGAAGCAGCGCGGCGTGGAAATCTTCACCCGCACCGACGGCGAACTGATCGCCCTGTTCAGCGAC
AAGTGGCGCCTATCCTTCATCGAGAAGGAGCACCCTGATTTCGTGCTGGAGTCGCTGGTAGCTGAGTAA

Upstream 100 bases:

>100_bases
CTCTATAGTTTTTAGAGTTTCTGTCAGCCTATAGGTAATTGTTTTTGAGCTTAAATGCAGTGACGTGTATTACATTTCAG
CCACCCCCGAATTAAGGGAA

Downstream 100 bases:

>100_bases
TTAACTGGGCGGCTTTGGTGGCTTGGGCTGCTTGGGATTTTTGCTTGTCGACGTCAGGTTGCAGGTGGCCCGGGCCCGGC
TGGCCAGTTCGGGGTGTGCT

Product: hypothetical protein

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MAKNVDKQATPACNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDAGKSTLTEALALHAHIISEAG
ATHGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGHEGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAK
GLEPQTLQLFRVCKARGLPIITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEAEEYIHF
TRTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIFASAMLNFGVHQILDTLCQLA
PSPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAGMDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYAL
TVFGRTRSTVETAFPGDIVGLVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQ
ILKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAKQRGVEIFTRTDGELIALFSD
KWRLSFIEKEHPDFVLESLVAE

Sequences:

>Translated_582_residues
MAKNVDKQATPACNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDAGKSTLTEALALHAHIISEAG
ATHGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGHEGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAK
GLEPQTLQLFRVCKARGLPIITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEAEEYIHF
TRTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIFASAMLNFGVHQILDTLCQLA
PSPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAGMDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYAL
TVFGRTRSTVETAFPGDIVGLVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQ
ILKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAKQRGVEIFTRTDGELIALFSD
KWRLSFIEKEHPDFVLESLVAE
>Mature_581_residues
AKNVDKQATPACNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDAGKSTLTEALALHAHIISEAGA
THGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGHEGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAKG
LEPQTLQLFRVCKARGLPIITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEAEEYIHFT
RTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIFASAMLNFGVHQILDTLCQLAP
SPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAGMDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYALT
VFGRTRSTVETAFPGDIVGLVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQI
LKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAKQRGVEIFTRTDGELIALFSDK
WRLSFIEKEHPDFVLESLVAE

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=529, Percent_Identity=24.7637051039698, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI19923640, Length=575, Percent_Identity=26.9565217391304, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI25306283, Length=412, Percent_Identity=28.6407766990291, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI25306287, Length=306, Percent_Identity=31.3725490196078, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157426893, Length=158, Percent_Identity=32.9113924050633, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=141, Percent_Identity=35.4609929078014, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=34.2281879194631, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=40.6909788867562, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789738, Length=529, Percent_Identity=27.0321361058601, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994988, Length=190, Percent_Identity=33.1578947368421, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=152, Percent_Identity=35.5263157894737, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=500, Percent_Identity=24.4, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17556745, Length=499, Percent_Identity=24.4488977955912, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=192, Percent_Identity=31.7708333333333, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=145, Percent_Identity=34.4827586206897, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71988811, Length=145, Percent_Identity=34.4827586206897, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=182, Percent_Identity=31.8681318681319, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=496, Percent_Identity=24.3951612903226, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322359, Length=553, Percent_Identity=24.9547920433996, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=34.1772151898734, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=37.5838926174497, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=37.5838926174497, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=273, Percent_Identity=27.8388278388278, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582462, Length=538, Percent_Identity=24.7211895910781, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI221458488, Length=539, Percent_Identity=24.1187384044527, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI78706572, Length=161, Percent_Identity=36.0248447204969, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=33.9869281045752, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=33.9869281045752, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=33.9869281045752, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28574573, Length=256, Percent_Identity=28.125, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 64030; Mature: 63899

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNVDKQATPACNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDA
CCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEECCCC
GKSTLTEALALHAHIISEAGATHGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGH
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHEEEEECCCCC
EGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAKGLEPQTLQLFRVCKARGLPI
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEE
ITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEAEEYIHF
EEEEHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEECCCCCHHHHEEE
TRTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIF
EECCCCCCCCCCCCCCCHHHHHCCCCHHCCCCHHHHHEECCCCCCCCEEEEECCCCHHHH
ASAMLNFGVHQILDTLCQLAPSPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
MDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYALTVFGRTRSTVETAFPGDIVG
CCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHCCCCCHHH
LVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQ
HHCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ILKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAK
HHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHEECHHHHCCCCCHHHHHHHHHHHHHHHHH
QRGVEIFTRTDGELIALFSDKWRLSFIEKEHPDFVLESLVAE
HCCCEEEEECCCCEEEEECCCEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKNVDKQATPACNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDA
CCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEECCCC
GKSTLTEALALHAHIISEAGATHGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGH
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHEEEEECCCCC
EGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAKGLEPQTLQLFRVCKARGLPI
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEE
ITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEAEEYIHF
EEEEHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEECCCCCHHHHEEE
TRTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIF
EECCCCCCCCCCCCCCCHHHHHCCCCHHCCCCHHHHHEECCCCCCCCEEEEECCCCHHHH
ASAMLNFGVHQILDTLCQLAPSPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
MDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYALTVFGRTRSTVETAFPGDIVG
CCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHCCCCCHHH
LVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQ
HHCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ILKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAK
HHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHEECHHHHCCCCCHHHHHHHHHHHHHHHHH
QRGVEIFTRTDGELIALFSDKWRLSFIEKEHPDFVLESLVAE
HCCCEEEEECCCCEEEEECCCEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA