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The map label for this gene is pth2 [H]

Identifier: 145295093

GI number: 145295093

Start: 1144626

End: 1145240

Strand: Reverse

Name: pth2 [H]

Synonym: cgR_1036

Alternate gene names: 145295093

Gene position: 1145240-1144626 (Counterclockwise)

Preceding gene: 145295096

Following gene: 145295087

Centisome position: 34.56

GC content: 58.54

Gene sequence:

>615_bases
ATGACTTCCGTGAGTTTTTTGTCTAAAATCCAAGCACTGTTTGCCCCCAAGCCTGAACTTCCCGCCGCCAAATGGCTGGT
CGTGGGCCTGGGCAACCCCGGCGCCAAGTACGAATCCACCCGGCACAACGTCGGTTACATGTGCCAAGACATGCTTATCG
ACGCCCACCAGCAGCAGCCCCTCACCCCTGCCACGGGCTACAAGGCCCTCACAACGCAGCTCGCACCAGAGGTGCTCGCC
GTTCGATCCACCACTTTTATGAACCACTCCGGCCAAGGTGTCGCACCGATCGCCGCAGCACTGGGTATCCCAGCAGAGCG
CATCATCGTGATCCACGACGAGCTCGATCTGCCCGCTGGAAAGGTACGTCTGAAAAAAGGAGGAAACGAAAACGGCCACA
ACGGGCTGAAATCCCTCACGGAAGAACTCGGCACCAGAGATTACCTGCGCGTCCGCATCGGAATTTCACGACCACCAGCA
GGAATGGCCGTGCCCGACTACGTTTTGGAACCAGTCGATCACGACCAACCAGGCATTGAGCTTGCCGCCGAGGCAGTGGA
TTTGCTGCTGGCCCAGGGATTATCTGCTGCGCAAAACGCTATCCACAGCCGCTAG

Upstream 100 bases:

>100_bases
GCGGGAGTGGACGGGCCGCCAGCCATCGGGGCGACAATCACGGGAGTTTTCAACGTGTCAAGGATGCTCATGTCACCATC
CTAGGCGCGCCTGCCATAGG

Downstream 100 bases:

>100_bases
ATTGCTAGAGATTCCCGCACTTTAGTCTGCTTCTCTGCGGTGGTTTCAGCTCCTTGTCCAGATTCCACGACCTGACCACG
CGCCACAATGGCGTAGCTTG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH 2 [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTSVSFLSKIQALFAPKPELPAAKWLVVGLGNPGAKYESTRHNVGYMCQDMLIDAHQQQPLTPATGYKALTTQLAPEVLA
VRSTTFMNHSGQGVAPIAAALGIPAERIIVIHDELDLPAGKVRLKKGGNENGHNGLKSLTEELGTRDYLRVRIGISRPPA
GMAVPDYVLEPVDHDQPGIELAAEAVDLLLAQGLSAAQNAIHSR

Sequences:

>Translated_204_residues
MTSVSFLSKIQALFAPKPELPAAKWLVVGLGNPGAKYESTRHNVGYMCQDMLIDAHQQQPLTPATGYKALTTQLAPEVLA
VRSTTFMNHSGQGVAPIAAALGIPAERIIVIHDELDLPAGKVRLKKGGNENGHNGLKSLTEELGTRDYLRVRIGISRPPA
GMAVPDYVLEPVDHDQPGIELAAEAVDLLLAQGLSAAQNAIHSR
>Mature_203_residues
TSVSFLSKIQALFAPKPELPAAKWLVVGLGNPGAKYESTRHNVGYMCQDMLIDAHQQQPLTPATGYKALTTQLAPEVLAV
RSTTFMNHSGQGVAPIAAALGIPAERIIVIHDELDLPAGKVRLKKGGNENGHNGLKSLTEELGTRDYLRVRIGISRPPAG
MAVPDYVLEPVDHDQPGIELAAEAVDLLLAQGLSAAQNAIHSR

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=167, Percent_Identity=35.3293413173653, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1787455, Length=187, Percent_Identity=39.0374331550802, Blast_Score=99, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321983, Length=148, Percent_Identity=34.4594594594595, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 21767; Mature: 21636

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVSFLSKIQALFAPKPELPAAKWLVVGLGNPGAKYESTRHNVGYMCQDMLIDAHQQQP
CCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
LTPATGYKALTTQLAPEVLAVRSTTFMNHSGQGVAPIAAALGIPAERIIVIHDELDLPAG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCC
KVRLKKGGNENGHNGLKSLTEELGTRDYLRVRIGISRPPAGMAVPDYVLEPVDHDQPGIE
EEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCHH
LAAEAVDLLLAQGLSAAQNAIHSR
HHHHHHHHHHHHCHHHHHHHHCCC
>Mature Secondary Structure 
TSVSFLSKIQALFAPKPELPAAKWLVVGLGNPGAKYESTRHNVGYMCQDMLIDAHQQQP
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
LTPATGYKALTTQLAPEVLAVRSTTFMNHSGQGVAPIAAALGIPAERIIVIHDELDLPAG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCC
KVRLKKGGNENGHNGLKSLTEELGTRDYLRVRIGISRPPAGMAVPDYVLEPVDHDQPGIE
EEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCHH
LAAEAVDLLLAQGLSAAQNAIHSR
HHHHHHHHHHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]