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The map label for this gene is ispE

Identifier: 145295069

GI number: 145295069

Start: 1119072

End: 1120010

Strand: Direct

Name: ispE

Synonym: cgR_1012

Alternate gene names: 145295069

Gene position: 1119072-1120010 (Clockwise)

Preceding gene: 145295068

Following gene: 145295070

Centisome position: 33.77

GC content: 60.81

Gene sequence:

>939_bases
GTGAAAATTACCGCTAAGGCGTGGGCGAAAACCAATTTGCATTTAGGTGTGGGACCGGCTCGCGACGATGGATTTCACGA
GCTCATGACGGTGTTTCAAACCATTGATCTGTTTGACACCGTCACCTTAACCACCCTCGATGAGGAGTTGGTGGAGGAGG
GGAGCGTCGTCAAGCAATTATCTGTGACCGGTGCCCGTGGCGTGCCCGAGGACGCCAGCAATCTTGCGTGGCGCGCTGTC
GATGCGTTGGTTAAGCGGCGCGCGGAAAAGACGCCGCTGTCTGCAGTTTCGCTGCATATTTCCAAGGGGATTCCGGTGGC
TGGCGGCATGGCTGGCGGCTCTGCGGATGCGGCTGCGACGCTGCGCGCGGTGGATGCCTGGATTGGGCCTTTCGGCGAGG
ACACATTGCTGGAGGTTGCCGCGGAGCTCGGCTCAGATGTGCCGTTTTGCCTGCTTGGTGGCACCATGCGCGGTACCGGT
CGCGGCGAGCAGCTGGTAGATATGTTGACGCGCGGCAAGCTACATTGGGTGGTGGCCGCGATGGCGCATGGCCTATCCAC
GCCTGAGGTATTCAAAAAGCATGATGAGCTGAATCCGGAATCGCATATGGACATCAGCGACCTCAGCGCCGCACTTCTCA
CCGGCAACACCGCCGAGGTGGGGCGGTGGCTGCACAACGATCTGACCAGCGCCGCACTCAGTTTGCGCCCTGAACTGCGC
AGCGTCCTCCAAGAAGGCATCCGCTCCGGCGCGCACGCAGGAATCGTCTCCGGCTCCGGCCCGACCACGGTATTCTTGTG
CGAATCGGAGCACAAAGCGCAAGACGTTAAAGAGGCGCTAATCGACGCCGGCCAGGTGTACGCTGCTTACACCGCTACCG
GCCCTGCGGCCTCAACCGCCGACCAGCGCGGCGCACACATTTTGACTGTTTCACAATAA

Upstream 100 bases:

>100_bases
CTTTGCGGGCCGCTGATATTGATCCAACGCTTCGTGGCGAAAAGCTTGATGTCACTGACTATGTGCGCCTAGCTGGGGTG
TTGCAGCAAAAGGATGAGAA

Downstream 100 bases:

>100_bases
AGACAAACTTAAGTATCGGAGTCGAAGAAAAACCACAATGGCCAATCTGATTAATCTCGAGAACGTCTCCAAAACCTGGG
GATTAAAAACGCTTCTCGAC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKITAKAWAKTNLHLGVGPARDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQLSVTGARGVPEDASNLAWRAV
DALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAATLRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTG
RGEQLVDMLTRGKLHWVVAAMAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGRWLHNDLTSAALSLRPELR
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTADQRGAHILTVSQ

Sequences:

>Translated_312_residues
MKITAKAWAKTNLHLGVGPARDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQLSVTGARGVPEDASNLAWRAV
DALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAATLRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTG
RGEQLVDMLTRGKLHWVVAAMAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGRWLHNDLTSAALSLRPELR
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTADQRGAHILTVSQ
>Mature_312_residues
MKITAKAWAKTNLHLGVGPARDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQLSVTGARGVPEDASNLAWRAV
DALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAATLRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTG
RGEQLVDMLTRGKLHWVVAAMAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGRWLHNDLTSAALSLRPELR
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTADQRGAHILTVSQ

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=196, Percent_Identity=33.1632653061224, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CORGB (A4QCP1)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137890.1
- ProteinModelPortal:   A4QCP1
- SMR:   A4QCP1
- STRING:   A4QCP1
- GeneID:   4992091
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1012
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK03188
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32805; Mature: 32805

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITAKAWAKTNLHLGVGPARDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQL
CCCCCCHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SVTGARGVPEDASNLAWRAVDALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAAT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHCCCCCCCCCCCCCCHHHHH
LRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTGRGEQLVDMLTRGKLHWVVAA
HHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHH
MAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGRWLHNDLTSAALSLRPELR
HHHCCCCHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHH
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTA
HHHHHHHHCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
DQRGAHILTVSQ
HHCCCEEEEECC
>Mature Secondary Structure
MKITAKAWAKTNLHLGVGPARDDGFHELMTVFQTIDLFDTVTLTTLDEELVEEGSVVKQL
CCCCCCHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SVTGARGVPEDASNLAWRAVDALVKRRAEKTPLSAVSLHISKGIPVAGGMAGGSADAAAT
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHCCCCCCCCCCCCCCHHHHH
LRAVDAWIGPFGEDTLLEVAAELGSDVPFCLLGGTMRGTGRGEQLVDMLTRGKLHWVVAA
HHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHH
MAHGLSTPEVFKKHDELNPESHMDISDLSAALLTGNTAEVGRWLHNDLTSAALSLRPELR
HHHCCCCHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHH
SVLQEGIRSGAHAGIVSGSGPTTVFLCESEHKAQDVKEALIDAGQVYAAYTATGPAASTA
HHHHHHHHCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
DQRGAHILTVSQ
HHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA