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The map label for this gene is ksgA

Identifier: 145295068

GI number: 145295068

Start: 1118194

End: 1119075

Strand: Direct

Name: ksgA

Synonym: cgR_1011

Alternate gene names: 145295068

Gene position: 1118194-1119075 (Clockwise)

Preceding gene: 145295067

Following gene: 145295069

Centisome position: 33.74

GC content: 55.78

Gene sequence:

>882_bases
ATGGAAGAACCCTCAGGTGCACAGCTGCTCGGCCCGGTAGAAATCCGTGCGCTGGCAGAAAAGCTCGACGTCACACCAAC
TAAGAAGTTGGGGCAGAACTTTGTTCACGATCCCAACACGGTGCGTCGCATTGTTGCTGCGGCAGAGCTCACCCCAGACG
ACCACGTGGTGGAAGTTGGCCCTGGTCTGGGCTCTCTGACCCTTGCCCTGGTGGAATCTGCTGCTTCAGTAACCGCGGTG
GAAATTGATCCCCGTTTGGCTGCGGAATTGCCGGAGACTTTTCAGTGGCGCGCGCCGGCCCTTGCTCACAAGTTGAGCAT
CGTGCTGAAAGACGCCCTGAAGGTGCAGCAATCTGACATGGAAGTCCAGCCCACCGCCTTGGTGGCTAACTTGCCGTACA
ACGTCTCTGTCCCTGTCTTGTTGCACATGTTGGAGGAGTTTCCCACCATCAACAAGGTGCTTGTCATGGTGCAGGCAGAG
GTTGCTGATCGTTTGGCAGCGGATCCAGGATCGAAGATTTATGGTGTGCCTAGCGTGAAGGCGTCCTTCTACGGTCCAGT
TACTCGCGCCGGGTCGATTGGTAAGAATGTCTTTTGGCCAGCTCCAAAGATCGAATCCGGTTTGGTGAAGATCGTGCGCG
AAGACACCGCGTGGAAGCAGGACGATGAGACGCGTAAGAAGGTGTGGCCGATCATTGATGCTGCTTTCTTGCAGCGCCGT
AAAACCCTAAGAGCTGCGCTTTCTGGACACTACGGTTCTGGCCAGGCAGCTGAGGAAGCTTTGCGGGCCGCTGATATTGA
TCCAACGCTTCGTGGCGAAAAGCTTGATGTCACTGACTATGTGCGCCTAGCTGGGGTGTTGCAGCAAAAGGATGAGAAGT
GA

Upstream 100 bases:

>100_bases
GGTTCAGGCTGCACAAGGTTGGGGAGCATGGCCTGCTTGCACCGCAAGCTTGGGCATCCGATAGTAGAAATCTGGCATCC
AATAGGTAGATTGGGATGCT

Downstream 100 bases:

>100_bases
AAATTACCGCTAAGGCGTGGGCGAAAACCAATTTGCATTTAGGTGTGGGACCGGCTCGCGACGATGGATTTCACGAGCTC
ATGACGGTGTTTCAAACCAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MEEPSGAQLLGPVEIRALAEKLDVTPTKKLGQNFVHDPNTVRRIVAAAELTPDDHVVEVGPGLGSLTLALVESAASVTAV
EIDPRLAAELPETFQWRAPALAHKLSIVLKDALKVQQSDMEVQPTALVANLPYNVSVPVLLHMLEEFPTINKVLVMVQAE
VADRLAADPGSKIYGVPSVKASFYGPVTRAGSIGKNVFWPAPKIESGLVKIVREDTAWKQDDETRKKVWPIIDAAFLQRR
KTLRAALSGHYGSGQAAEEALRAADIDPTLRGEKLDVTDYVRLAGVLQQKDEK

Sequences:

>Translated_293_residues
MEEPSGAQLLGPVEIRALAEKLDVTPTKKLGQNFVHDPNTVRRIVAAAELTPDDHVVEVGPGLGSLTLALVESAASVTAV
EIDPRLAAELPETFQWRAPALAHKLSIVLKDALKVQQSDMEVQPTALVANLPYNVSVPVLLHMLEEFPTINKVLVMVQAE
VADRLAADPGSKIYGVPSVKASFYGPVTRAGSIGKNVFWPAPKIESGLVKIVREDTAWKQDDETRKKVWPIIDAAFLQRR
KTLRAALSGHYGSGQAAEEALRAADIDPTLRGEKLDVTDYVRLAGVLQQKDEK
>Mature_293_residues
MEEPSGAQLLGPVEIRALAEKLDVTPTKKLGQNFVHDPNTVRRIVAAAELTPDDHVVEVGPGLGSLTLALVESAASVTAV
EIDPRLAAELPETFQWRAPALAHKLSIVLKDALKVQQSDMEVQPTALVANLPYNVSVPVLLHMLEEFPTINKVLVMVQAE
VADRLAADPGSKIYGVPSVKASFYGPVTRAGSIGKNVFWPAPKIESGLVKIVREDTAWKQDDETRKKVWPIIDAAFLQRR
KTLRAALSGHYGSGQAAEEALRAADIDPTLRGEKLDVTDYVRLAGVLQQKDEK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=219, Percent_Identity=36.0730593607306, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI156415992, Length=298, Percent_Identity=29.5302013422819, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=33.8345864661654, Blast_Score=140, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25146882, Length=181, Percent_Identity=36.4640883977901, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=290, Percent_Identity=27.2413793103448, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=182, Percent_Identity=36.2637362637363, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21358017, Length=235, Percent_Identity=33.6170212765957, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21357273, Length=305, Percent_Identity=27.5409836065574, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CORGB (A4QCP0)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137889.1
- ProteinModelPortal:   A4QCP0
- SMR:   A4QCP0
- STRING:   A4QCP0
- GeneID:   4992120
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1011
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31814; Mature: 31814

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 33-33 BINDING 35-35 BINDING 60-60 BINDING 81-81 BINDING 111-111 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEPSGAQLLGPVEIRALAEKLDVTPTKKLGQNFVHDPNTVRRIVAAAELTPDDHVVEVG
CCCCCCCCEECHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEC
PGLGSLTLALVESAASVTAVEIDPRLAAELPETFQWRAPALAHKLSIVLKDALKVQQSDM
CCCHHHHHHHHHHHHCEEEEEECCHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
EVQPTALVANLPYNVSVPVLLHMLEEFPTINKVLVMVQAEVADRLAADPGSKIYGVPSVK
CCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
ASFYGPVTRAGSIGKNVFWPAPKIESGLVKIVREDTAWKQDDETRKKVWPIIDAAFLQRR
HHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KTLRAALSGHYGSGQAAEEALRAADIDPTLRGEKLDVTDYVRLAGVLQQKDEK
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEEPSGAQLLGPVEIRALAEKLDVTPTKKLGQNFVHDPNTVRRIVAAAELTPDDHVVEVG
CCCCCCCCEECHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEC
PGLGSLTLALVESAASVTAVEIDPRLAAELPETFQWRAPALAHKLSIVLKDALKVQQSDM
CCCHHHHHHHHHHHHCEEEEEECCHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
EVQPTALVANLPYNVSVPVLLHMLEEFPTINKVLVMVQAEVADRLAADPGSKIYGVPSVK
CCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
ASFYGPVTRAGSIGKNVFWPAPKIESGLVKIVREDTAWKQDDETRKKVWPIIDAAFLQRR
HHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KTLRAALSGHYGSGQAAEEALRAADIDPTLRGEKLDVTDYVRLAGVLQQKDEK
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA