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The map label for this gene is purN [H]

Identifier: 145295031

GI number: 145295031

Start: 1083889

End: 1084521

Strand: Direct

Name: purN [H]

Synonym: cgR_0975

Alternate gene names: 145295031

Gene position: 1083889-1084521 (Clockwise)

Preceding gene: 145295030

Following gene: 145295032

Centisome position: 32.7

GC content: 51.34

Gene sequence:

>633_bases
ATGATGGTTCCGAGGCCGAAGACCGTTAACATATCTGTTGTGAATTCTGACTCTACGACCACCATTGTCGTGCTAGCTTC
CGGAACAGGCACCCTCCTTCAGTCACTCATTGAAGCGCAAGGTACCTATTCGATCGTGGGCGTTGTCTCTGACGTCGAAT
GCCCTGCACTTTCCAGAGCCGCAGATGCAGGTATTGATACTGCAGTTGTTCCGCTTGGAAAAGATCGTGCACAGTGGAAC
CACGAGCTTGCAGACGCAGTTGCAGTAAGCGACCCAGATTTGGTGGTCTCTGCGGGATTCATGAAAATTTTGGGCGAAGG
TTTCCTCTCAAGGTTCCCGTCCCGCATCATCAACACCCACCCAGCTTTATTGCCTTCTTTCCCTGGTGCCCACGCGGTTC
GCGATGCTTTGGCATACGGTGTGAAAGTGTCAGGTTCGACAGTTCACCTTGTCGATGCTGGTGTGGATACCGGCCCAATT
ATTGCTCAACGAGCAGTGCCGGTAGAAGTGAATGATGATGAATCCAGCCTGCATGAAAGAATCAAGCAGGTTGAGCGTAA
ACTCATTGTAGAAGTCCTGAACAGCGTGGAATTTTCGCGTCAGGGTGGCGTACAACTCAACTGGAGAGGCTAA

Upstream 100 bases:

>100_bases
GAAGAGGTCGAAGAAGACGACGCAGAGGATCCTGAAGAGAATCCTGATGAGGAAGAATCCGACGAAGAAATTGAGCCAGA
AACTGAGGCTGAAGAAACCA

Downstream 100 bases:

>100_bases
ATCCTTCATGAGCGATGATCGTAAGGCAATTAAACGCGCACTAATTAGCGTGTATGACAAGACTGGCCTGGAGGATCTAG
CCCAGGCACTTCACCGCGAG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MMVPRPKTVNISVVNSDSTTTIVVLASGTGTLLQSLIEAQGTYSIVGVVSDVECPALSRAADAGIDTAVVPLGKDRAQWN
HELADAVAVSDPDLVVSAGFMKILGEGFLSRFPSRIINTHPALLPSFPGAHAVRDALAYGVKVSGSTVHLVDAGVDTGPI
IAQRAVPVEVNDDESSLHERIKQVERKLIVEVLNSVEFSRQGGVQLNWRG

Sequences:

>Translated_210_residues
MMVPRPKTVNISVVNSDSTTTIVVLASGTGTLLQSLIEAQGTYSIVGVVSDVECPALSRAADAGIDTAVVPLGKDRAQWN
HELADAVAVSDPDLVVSAGFMKILGEGFLSRFPSRIINTHPALLPSFPGAHAVRDALAYGVKVSGSTVHLVDAGVDTGPI
IAQRAVPVEVNDDESSLHERIKQVERKLIVEVLNSVEFSRQGGVQLNWRG
>Mature_210_residues
MMVPRPKTVNISVVNSDSTTTIVVLASGTGTLLQSLIEAQGTYSIVGVVSDVECPALSRAADAGIDTAVVPLGKDRAQWN
HELADAVAVSDPDLVVSAGFMKILGEGFLSRFPSRIINTHPALLPSFPGAHAVRDALAYGVKVSGSTVHLVDAGVDTGPI
IAQRAVPVEVNDDESSLHERIKQVERKLIVEVLNSVEFSRQGGVQLNWRG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=219, Percent_Identity=37.8995433789954, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI209869995, Length=219, Percent_Identity=37.8995433789954, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI209869993, Length=219, Percent_Identity=37.8995433789954, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788846, Length=175, Percent_Identity=38.8571428571429, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787483, Length=124, Percent_Identity=35.4838709677419, Blast_Score=88, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=173, Percent_Identity=45.6647398843931, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=205, Percent_Identity=33.1707317073171, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24582400, Length=180, Percent_Identity=42.7777777777778, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22201; Mature: 22201

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVPRPKTVNISVVNSDSTTTIVVLASGTGTLLQSLIEAQGTYSIVGVVSDVECPALSRA
CCCCCCCEEEEEEEECCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHH
ADAGIDTAVVPLGKDRAQWNHELADAVAVSDPDLVVSAGFMKILGEGFLSRFPSRIINTH
HHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
PALLPSFPGAHAVRDALAYGVKVSGSTVHLVDAGVDTGPIIAQRAVPVEVNDDESSLHER
CCCCCCCCCHHHHHHHHHHCEEECCCEEEEEECCCCCCCEEECCCEEEEECCCHHHHHHH
IKQVERKLIVEVLNSVEFSRQGGVQLNWRG
HHHHHHHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MMVPRPKTVNISVVNSDSTTTIVVLASGTGTLLQSLIEAQGTYSIVGVVSDVECPALSRA
CCCCCCCEEEEEEEECCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHH
ADAGIDTAVVPLGKDRAQWNHELADAVAVSDPDLVVSAGFMKILGEGFLSRFPSRIINTH
HHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
PALLPSFPGAHAVRDALAYGVKVSGSTVHLVDAGVDTGPIIAQRAVPVEVNDDESSLHER
CCCCCCCCCHHHHHHHHHHCEEECCCEEEEEECCCCCCCEEECCCEEEEECCCHHHHHHH
IKQVERKLIVEVLNSVEFSRQGGVQLNWRG
HHHHHHHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]