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The map label for this gene is proP [H]

Identifier: 145294821

GI number: 145294821

Start: 847458

End: 848822

Strand: Reverse

Name: proP [H]

Synonym: cgR_0768

Alternate gene names: 145294821

Gene position: 848822-847458 (Counterclockwise)

Preceding gene: 145294822

Following gene: 145294820

Centisome position: 25.61

GC content: 49.3

Gene sequence:

>1365_bases
ATGAGCATGAATAACTCTTTGACTGTAGAGGCCTCCAAAACAGGACTTCGGCGAAATATCTTCGCTGGCGCAATTGGTGT
TTTGGTGCACTGGTTTGACTGGGCGGTATACGCATACCTAGCCACCACTATTTCTCACGTGTTCTTCCCGGAACAAAGTG
AAACTGCAGCACTTCTATCCGTTTTCGCAGTTTTTGCCGTGGCTTTCTTTGTGCGTCCGCTCGGATCACTCATTTTCGGC
CATCTCGGTGACACCCTCGGGCGAAAGAAGACCCTGTCGCTCGTCATCATCATGATGGCTGCTGGAACTTTGATGCTGGG
ACTGATCCCTTCTCACGAAACTATTGGAATCTGGGCCCCAGTGCTACTCATCGTGGCGCGAGTAATCCAGGGTATTGCCG
CAGGTGGTGAGTTTGGCTCCGCAGCCGCATTTCTAGCTGAATACTCGCCTCCAAAGAAACGTGGATTTGGCTGTTCTTGG
ATCGAATTTGGTTCAGTTCTCGGCTTTTTGTTGGCTTCAGTTACTGTCGCCGGTCTTCACATGCTTTTTGATGACGAGAC
CATTGCAAACGGTGCGTGGAGAATTCCGTTCCTTCTGACTGTCCCTCTGGCAGCCGTTGGACTTTATATTCGATTGAAAA
TTGAAGACACCCCTGAGTACCAAGCACTTCAAGATATGGATACTGTTCCTGCTGCACCAATTCGTGAAGTATTCAAGTAC
AACAAGACCCAGTTTTTTCAAACAATCGGTGTCGAAATCTTCATGAACTGTACGTTCTATGTCGTACTCGTTTATCTGAT
TACTTACCAAGAATCCATGATTGGGATGTCTGCTGGCCAAGCCGCGACATTGTCGACAATTGCCTCTCTTACCGCTGTCG
CTATCATCCCTCTCGCTGGAATCGCCTCAGACAAGATCGGCCGCAAGCCAGTCCTGATCACCGCAGGTGTTTTACTTGTT
GCTCTTGCTTGGCCACTTTTCACTTACATGAATCAAGGAACTCCGATGGCTGGTTTTATCTCCACCTTCGGACTCGCTTC
GATTCTGTCGCTGATCTTAGGCACCCATGCCGCAACAGTCGCAGAACTGTTCCCTACCCGAACTCGCCAATCAGGACTTT
CTATCGCATATTCGATTGCCGGAGCATTTTTCGCTGGCACGCTGCCCTACATCAACACCTGGCTAATCAGTGTCACTGGA
AATCACATGATCCCTGCCTATACATTGATGGTAGTTGGAGGAATCGGTCTGATTACTCTGTTCACAATGCCTGAGACCGC
CGGCAGAAATCTCCTGCATGAATCAGACCTGGCTGCTGCGAAAAACCAGACTCCTGAGTTTCACCGAATGAAGGAAGGTG
TCTAG

Upstream 100 bases:

>100_bases
AAGGGGTCTCAGGTTTAAGCGATAGACGTGAGCTGAATCTCTTTTTAAGGTTCAAACACAAAGGACCACATAAAGAGCCC
GACTCTGAAAGGAACAGGTA

Downstream 100 bases:

>100_bases
CTGCTAGATTTCCAGATAAGAAACTAAAATCGCCATTCCCAGATCACAGCTTTCGTGGTCTGGGTGCTTTCTATTTCAAG
CGCCTCCGGGAGGCATTATG

Product: hypothetical protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSMNNSLTVEASKTGLRRNIFAGAIGVLVHWFDWAVYAYLATTISHVFFPEQSETAALLSVFAVFAVAFFVRPLGSLIFG
HLGDTLGRKKTLSLVIIMMAAGTLMLGLIPSHETIGIWAPVLLIVARVIQGIAAGGEFGSAAAFLAEYSPPKKRGFGCSW
IEFGSVLGFLLASVTVAGLHMLFDDETIANGAWRIPFLLTVPLAAVGLYIRLKIEDTPEYQALQDMDTVPAAPIREVFKY
NKTQFFQTIGVEIFMNCTFYVVLVYLITYQESMIGMSAGQAATLSTIASLTAVAIIPLAGIASDKIGRKPVLITAGVLLV
ALAWPLFTYMNQGTPMAGFISTFGLASILSLILGTHAATVAELFPTRTRQSGLSIAYSIAGAFFAGTLPYINTWLISVTG
NHMIPAYTLMVVGGIGLITLFTMPETAGRNLLHESDLAAAKNQTPEFHRMKEGV

Sequences:

>Translated_454_residues
MSMNNSLTVEASKTGLRRNIFAGAIGVLVHWFDWAVYAYLATTISHVFFPEQSETAALLSVFAVFAVAFFVRPLGSLIFG
HLGDTLGRKKTLSLVIIMMAAGTLMLGLIPSHETIGIWAPVLLIVARVIQGIAAGGEFGSAAAFLAEYSPPKKRGFGCSW
IEFGSVLGFLLASVTVAGLHMLFDDETIANGAWRIPFLLTVPLAAVGLYIRLKIEDTPEYQALQDMDTVPAAPIREVFKY
NKTQFFQTIGVEIFMNCTFYVVLVYLITYQESMIGMSAGQAATLSTIASLTAVAIIPLAGIASDKIGRKPVLITAGVLLV
ALAWPLFTYMNQGTPMAGFISTFGLASILSLILGTHAATVAELFPTRTRQSGLSIAYSIAGAFFAGTLPYINTWLISVTG
NHMIPAYTLMVVGGIGLITLFTMPETAGRNLLHESDLAAAKNQTPEFHRMKEGV
>Mature_453_residues
SMNNSLTVEASKTGLRRNIFAGAIGVLVHWFDWAVYAYLATTISHVFFPEQSETAALLSVFAVFAVAFFVRPLGSLIFGH
LGDTLGRKKTLSLVIIMMAAGTLMLGLIPSHETIGIWAPVLLIVARVIQGIAAGGEFGSAAAFLAEYSPPKKRGFGCSWI
EFGSVLGFLLASVTVAGLHMLFDDETIANGAWRIPFLLTVPLAAVGLYIRLKIEDTPEYQALQDMDTVPAAPIREVFKYN
KTQFFQTIGVEIFMNCTFYVVLVYLITYQESMIGMSAGQAATLSTIASLTAVAIIPLAGIASDKIGRKPVLITAGVLLVA
LAWPLFTYMNQGTPMAGFISTFGLASILSLILGTHAATVAELFPTRTRQSGLSIAYSIAGAFFAGTLPYINTWLISVTGN
HMIPAYTLMVVGGIGLITLFTMPETAGRNLLHESDLAAAKNQTPEFHRMKEGV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=450, Percent_Identity=36.4444444444444, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1788292, Length=399, Percent_Identity=31.077694235589, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788942, Length=388, Percent_Identity=33.5051546391753, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1789941, Length=424, Percent_Identity=32.0754716981132, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI87082231, Length=218, Percent_Identity=25.2293577981651, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48795; Mature: 48664

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNNSLTVEASKTGLRRNIFAGAIGVLVHWFDWAVYAYLATTISHVFFPEQSETAALLS
CCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VFAVFAVAFFVRPLGSLIFGHLGDTLGRKKTLSLVIIMMAAGTLMLGLIPSHETIGIWAP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHH
VLLIVARVIQGIAAGGEFGSAAAFLAEYSPPKKRGFGCSWIEFGSVLGFLLASVTVAGLH
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MLFDDETIANGAWRIPFLLTVPLAAVGLYIRLKIEDTPEYQALQDMDTVPAAPIREVFKY
HHCCCCHHCCCCEECHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHC
NKTQFFQTIGVEIFMNCTFYVVLVYLITYQESMIGMSAGQAATLSTIASLTAVAIIPLAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
IASDKIGRKPVLITAGVLLVALAWPLFTYMNQGTPMAGFISTFGLASILSLILGTHAATV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AELFPTRTRQSGLSIAYSIAGAFFAGTLPYINTWLISVTGNHMIPAYTLMVVGGIGLITL
HHHHCCHHHHCCCHHHHHHHHHHHHCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHE
FTMPETAGRNLLHESDLAAAKNQTPEFHRMKEGV
EECCCHHCCCHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
SMNNSLTVEASKTGLRRNIFAGAIGVLVHWFDWAVYAYLATTISHVFFPEQSETAALLS
CCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VFAVFAVAFFVRPLGSLIFGHLGDTLGRKKTLSLVIIMMAAGTLMLGLIPSHETIGIWAP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHH
VLLIVARVIQGIAAGGEFGSAAAFLAEYSPPKKRGFGCSWIEFGSVLGFLLASVTVAGLH
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MLFDDETIANGAWRIPFLLTVPLAAVGLYIRLKIEDTPEYQALQDMDTVPAAPIREVFKY
HHCCCCHHCCCCEECHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHC
NKTQFFQTIGVEIFMNCTFYVVLVYLITYQESMIGMSAGQAATLSTIASLTAVAIIPLAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
IASDKIGRKPVLITAGVLLVALAWPLFTYMNQGTPMAGFISTFGLASILSLILGTHAATV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AELFPTRTRQSGLSIAYSIAGAFFAGTLPYINTWLISVTGNHMIPAYTLMVVGGIGLITL
HHHHCCHHHHCCCHHHHHHHHHHHHCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHE
FTMPETAGRNLLHESDLAAAKNQTPEFHRMKEGV
EECCCHHCCCHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]