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The map label for this gene is thcD [H]

Identifier: 145294713

GI number: 145294713

Start: 718735

End: 720003

Strand: Reverse

Name: thcD [H]

Synonym: cgR_0661

Alternate gene names: 145294713

Gene position: 720003-718735 (Counterclockwise)

Preceding gene: 145294714

Following gene: 145294712

Centisome position: 21.72

GC content: 54.69

Gene sequence:

>1269_bases
ATGAATACTTCAGCTGAAACTGGAATCTTGATCATCGGCGCAAACCAATCGGGTGTGCAGCTGGCAATTTCCCTGCGGGC
CACGGGTTTCACCGAGTCGATCACGCTTCTGGGCGAGGAGGATCACCGCCCCTACCAGCGTCCCGCCTTGTCCAAGGAGT
TCCTCCAGGACAAGATCGACAAGGAGCGTCTGATTTTCCGTTCCAATGAGTATTGGGAAGAAAATAACATTCGCCTGGTC
AAGGGCGTGCGCATCGAACGCATTGAAAAGAACGACGACGGATCAGGGGTCGCCTACGGCGCCGGACAAGAATTCGCTTT
TCGACGTCTCGCTCTAGCGGTTGGTGCCCGCCCTCGCCACCTCGACCTCCCGGGCGCCACCTTTGAGGGTGTCACCTACC
TGCGCAACGCGGACGACGCCTTGGCGCTCAAAGCGATGATTGGTTCTGTCACCGATGCCGTTGTAGTCGGTGGTGGGTTC
ATCGGATTGGAAGCTGCGTGTTCGCTTCATGACCTCGGCAAAAATGTCACCGTCCTGGAATATGGTCCGCGTCTGATTGG
CCGAGCGGTGGGTGAAGAAACCGCAGCATTCTTCCTCGAACAACACCGTTCCCGTGGCGTAAATATCGTGCTTGATGCCC
GCATGAAACAGTTTGTGGGCAAGGATGGAAAGCTCAGCGGCGTTGAGCTAGAAGATGGCACAGTAATTCCTGCCCAACTA
GTCATTGTGGGCATCGGTGTCATTCCGAACACAGAACTTGCCACTGATCTGGGCTTAGACATCAACAACGGCATCGTGGT
GGATAAACATGCCGTCGCGTCAGATGGCACCACCATTGCGATTGGCGATGTCGCCAACATTCCCAATCCAATCCCTGGTT
CCCCCGCTGATGAACGCATCCGACTAGAAAGCGTCAATAACGCCATCGAGCACGCGAAGATCGCTGCATACTCACTCGTC
GGCCAGCCCGAAGCCTACGCCGGAATCCCCTGGTTCTGGTCCAACCAAGGCGATCTCAAACTACAAATTGCAGGACTTAC
CGTTGGTTATGACAGCACAGTAATCCGACAGGATCCCGAGAAAAAGAAGTTCTCTGTCCTTTACTACCGCGGCGACAACA
TCATTGCCGCCGATTGTGTCAATGCGCCACTAGATTTCATGGCTGTGCGCAGTGCACTTTCCAAGAATCAAAATATCCCC
GCCGATCTTGCTGCAGATATTTCGCAGCCGCTGAAAAAGCTAGCCGTTGACCTGGAGGTTACCCGATGA

Upstream 100 bases:

>100_bases
GGACCGTGAGGATTGCTCCCGTTTGTCTTGCCAAATCAAGGTCACCGAAGGCATGGATCTTTCGGTGACCACGCCAGAAA
CGCAAGTGTGAGGTTGAATC

Downstream 100 bases:

>100_bases
CTCGCAGTAATTTACCCGCTTGGGAGCAAGCAGACCCCAGCGTCCACGCCTCTGATCCCCGCGCCATCACTTTTGCTGAA
GATTTTAGGATTCGTCCCAT

Product: hypothetical protein

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNTSAETGILIIGANQSGVQLAISLRATGFTESITLLGEEDHRPYQRPALSKEFLQDKIDKERLIFRSNEYWEENNIRLV
KGVRIERIEKNDDGSGVAYGAGQEFAFRRLALAVGARPRHLDLPGATFEGVTYLRNADDALALKAMIGSVTDAVVVGGGF
IGLEAACSLHDLGKNVTVLEYGPRLIGRAVGEETAAFFLEQHRSRGVNIVLDARMKQFVGKDGKLSGVELEDGTVIPAQL
VIVGIGVIPNTELATDLGLDINNGIVVDKHAVASDGTTIAIGDVANIPNPIPGSPADERIRLESVNNAIEHAKIAAYSLV
GQPEAYAGIPWFWSNQGDLKLQIAGLTVGYDSTVIRQDPEKKKFSVLYYRGDNIIAADCVNAPLDFMAVRSALSKNQNIP
ADLAADISQPLKKLAVDLEVTR

Sequences:

>Translated_422_residues
MNTSAETGILIIGANQSGVQLAISLRATGFTESITLLGEEDHRPYQRPALSKEFLQDKIDKERLIFRSNEYWEENNIRLV
KGVRIERIEKNDDGSGVAYGAGQEFAFRRLALAVGARPRHLDLPGATFEGVTYLRNADDALALKAMIGSVTDAVVVGGGF
IGLEAACSLHDLGKNVTVLEYGPRLIGRAVGEETAAFFLEQHRSRGVNIVLDARMKQFVGKDGKLSGVELEDGTVIPAQL
VIVGIGVIPNTELATDLGLDINNGIVVDKHAVASDGTTIAIGDVANIPNPIPGSPADERIRLESVNNAIEHAKIAAYSLV
GQPEAYAGIPWFWSNQGDLKLQIAGLTVGYDSTVIRQDPEKKKFSVLYYRGDNIIAADCVNAPLDFMAVRSALSKNQNIP
ADLAADISQPLKKLAVDLEVTR
>Mature_422_residues
MNTSAETGILIIGANQSGVQLAISLRATGFTESITLLGEEDHRPYQRPALSKEFLQDKIDKERLIFRSNEYWEENNIRLV
KGVRIERIEKNDDGSGVAYGAGQEFAFRRLALAVGARPRHLDLPGATFEGVTYLRNADDALALKAMIGSVTDAVVVGGGF
IGLEAACSLHDLGKNVTVLEYGPRLIGRAVGEETAAFFLEQHRSRGVNIVLDARMKQFVGKDGKLSGVELEDGTVIPAQL
VIVGIGVIPNTELATDLGLDINNGIVVDKHAVASDGTTIAIGDVANIPNPIPGSPADERIRLESVNNAIEHAKIAAYSLV
GQPEAYAGIPWFWSNQGDLKLQIAGLTVGYDSTVIRQDPEKKKFSVLYYRGDNIIAADCVNAPLDFMAVRSALSKNQNIP
ADLAADISQPLKKLAVDLEVTR

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=380, Percent_Identity=27.3684210526316, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI226437568, Length=380, Percent_Identity=27.3684210526316, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI21389617, Length=380, Percent_Identity=27.3684210526316, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI4757732, Length=379, Percent_Identity=26.1213720316623, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI22202629, Length=379, Percent_Identity=25.5936675461741, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788892, Length=409, Percent_Identity=32.7628361858191, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789065, Length=282, Percent_Identity=26.241134751773, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=384, Percent_Identity=26.3020833333333, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24639257, Length=420, Percent_Identity=26.6666666666667, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI281359715, Length=420, Percent_Identity=26.6666666666667, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI281359713, Length=420, Percent_Identity=26.6666666666667, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24639250, Length=420, Percent_Identity=26.6666666666667, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI18543267, Length=420, Percent_Identity=26.6666666666667, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24639252, Length=420, Percent_Identity=26.6666666666667, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24585130, Length=336, Percent_Identity=25.5952380952381, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24581020, Length=366, Percent_Identity=24.0437158469945, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28573993, Length=366, Percent_Identity=24.0437158469945, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 45467; Mature: 45467

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSAETGILIIGANQSGVQLAISLRATGFTESITLLGEEDHRPYQRPALSKEFLQDKID
CCCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCC
KERLIFRSNEYWEENNIRLVKGVRIERIEKNDDGSGVAYGAGQEFAFRRLALAVGARPRH
HHHEEEECCCCCCCCCEEEEECCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCE
LDLPGATFEGVTYLRNADDALALKAMIGSVTDAVVVGGGFIGLEAACSLHDLGKNVTVLE
ECCCCCCCCCCCEECCCCHHHHHHHHHHHHCCEEEECCCEECHHHHHHHHHCCCCEEEEE
YGPRLIGRAVGEETAAFFLEQHRSRGVNIVLDARMKQFVGKDGKLSGVELEDGTVIPAQL
CCCHHHHHHHCHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCEEECCCEECCEEE
VIVGIGVIPNTELATDLGLDINNGIVVDKHAVASDGTTIAIGDVANIPNPIPGSPADERI
EEEEECCCCCCCHHHHCCCCCCCCEEEECCEECCCCCEEEECCCCCCCCCCCCCCCCCEE
RLESVNNAIEHAKIAAYSLVGQPEAYAGIPWFWSNQGDLKLQIAGLTVGYDSTVIRQDPE
EHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEECCCCCEEEEEEEEEECCCCEEECCCCC
KKKFSVLYYRGDNIIAADCVNAPLDFMAVRSALSKNQNIPADLAADISQPLKKLAVDLEV
CCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEE
TR
CC
>Mature Secondary Structure
MNTSAETGILIIGANQSGVQLAISLRATGFTESITLLGEEDHRPYQRPALSKEFLQDKID
CCCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCC
KERLIFRSNEYWEENNIRLVKGVRIERIEKNDDGSGVAYGAGQEFAFRRLALAVGARPRH
HHHEEEECCCCCCCCCEEEEECCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCE
LDLPGATFEGVTYLRNADDALALKAMIGSVTDAVVVGGGFIGLEAACSLHDLGKNVTVLE
ECCCCCCCCCCCEECCCCHHHHHHHHHHHHCCEEEECCCEECHHHHHHHHHCCCCEEEEE
YGPRLIGRAVGEETAAFFLEQHRSRGVNIVLDARMKQFVGKDGKLSGVELEDGTVIPAQL
CCCHHHHHHHCHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCEEECCCEECCEEE
VIVGIGVIPNTELATDLGLDINNGIVVDKHAVASDGTTIAIGDVANIPNPIPGSPADERI
EEEEECCCCCCCHHHHCCCCCCCCEEEECCEECCCCCEEEECCCCCCCCCCCCCCCCCEE
RLESVNNAIEHAKIAAYSLVGQPEAYAGIPWFWSNQGDLKLQIAGLTVGYDSTVIRQDPE
EHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEECCCCCEEEEEEEEEECCCCEEECCCCC
KKKFSVLYYRGDNIIAADCVNAPLDFMAVRSALSKNQNIPADLAADISQPLKKLAVDLEV
CCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEE
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]