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The map label for this gene is lip2 [H]

Identifier: 145294238

GI number: 145294238

Start: 211847

End: 212764

Strand: Reverse

Name: lip2 [H]

Synonym: cgR_0194

Alternate gene names: 145294238

Gene position: 212764-211847 (Counterclockwise)

Preceding gene: 145294241

Following gene: 145294236

Centisome position: 6.42

GC content: 54.14

Gene sequence:

>918_bases
ATGTTCCTTCATCCAGATGCGCAGTTTTATATTGATACTTTGTCTACTCTCAGCGCGGAGGAGCAGGTGAATTTTGGCAA
AGACGCTGTTGGGTCGGGAGTTGATGCAACTCATTCGGCGGCGGATCAAGATATTGCTGGGGTGCCGGTGAGGGTTTATA
CACCTTTATCTGGGGCTGGGGATTTTCCGTGCTTGGTGTATTTTCACGGCGGAGGTTGGTCCGGTGGCACCCTAGACATG
ATCGATGCCACGGTTCGCTCCCTAGTTGTCGGCCTGCCGATCATCGCCATCAGCGTGGACTACCGACTTGCACCCGCACA
CCCATTTCCAGCGGCTATCGACGATGCGTTTGTGGTGGCCAGCGCAGTGTTGGATGGGGTGTCTGGGCTGAGTATTGATA
CTTCCCGAGTGGCAATTGGCGGTGACAGTGCCGGTGGAAATATTGCCGCAGTTACTGCACAGCAGCTGCGTGAACGGGCT
GTGGATTCTGTTTCTGTGTCTGGGTTGGCGACTGCATTGGCTCGCCAGGTGCTTATTTTTCCGGTAACTGATGTTTCCAC
CACGGATACGCCGAGCTATCTCACATTTGGCAAAGATTGCTACCTGACAAAGGACGCGATGGAACGCTACATCGAACAAT
ATGCAGATGGGCACGACCGCACCGACCCTCGACTCTCTCCGCTACTGGCCTCTGATTTGAGCGGACTGCCACCAACCACC
ATTGTGTACGGCGAATGCGACGTGTTAGCCCATGAAGTGCGAGCCTATGGACAAGCTCTACTAGAGGCTGGAAATTCCGT
GACGATTACTGAATTCATGGGTCAGATCCACGCCTTTATTAACCTAGGGGGAATCAGTTCCGATGCGCGGGCTGCTCGAC
GACTCATCCGCGCCGAATTGGAAGCAGCGCTTTGTTAA

Upstream 100 bases:

>100_bases
TTCTAACCTGCGTCCAAGCCTAGGTGGAATTGAGATGACGCGTCGTGGAGATCGGGAATTTGCTGAAATTTATTGACCGA
AGGCTTCTAGGATTGTCGTT

Downstream 100 bases:

>100_bases
AGGTTCAGATTTAACATTCGCCGGGTTCCACTTCCCAAGAAATACGGATCATTTCCTCAAGCACCTCAAGATCAACATCC
TCTGGTTTGTTGATATATAG

Product: hypothetical protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MFLHPDAQFYIDTLSTLSAEEQVNFGKDAVGSGVDATHSAADQDIAGVPVRVYTPLSGAGDFPCLVYFHGGGWSGGTLDM
IDATVRSLVVGLPIIAISVDYRLAPAHPFPAAIDDAFVVASAVLDGVSGLSIDTSRVAIGGDSAGGNIAAVTAQQLRERA
VDSVSVSGLATALARQVLIFPVTDVSTTDTPSYLTFGKDCYLTKDAMERYIEQYADGHDRTDPRLSPLLASDLSGLPPTT
IVYGECDVLAHEVRAYGQALLEAGNSVTITEFMGQIHAFINLGGISSDARAARRLIRAELEAALC

Sequences:

>Translated_305_residues
MFLHPDAQFYIDTLSTLSAEEQVNFGKDAVGSGVDATHSAADQDIAGVPVRVYTPLSGAGDFPCLVYFHGGGWSGGTLDM
IDATVRSLVVGLPIIAISVDYRLAPAHPFPAAIDDAFVVASAVLDGVSGLSIDTSRVAIGGDSAGGNIAAVTAQQLRERA
VDSVSVSGLATALARQVLIFPVTDVSTTDTPSYLTFGKDCYLTKDAMERYIEQYADGHDRTDPRLSPLLASDLSGLPPTT
IVYGECDVLAHEVRAYGQALLEAGNSVTITEFMGQIHAFINLGGISSDARAARRLIRAELEAALC
>Mature_305_residues
MFLHPDAQFYIDTLSTLSAEEQVNFGKDAVGSGVDATHSAADQDIAGVPVRVYTPLSGAGDFPCLVYFHGGGWSGGTLDM
IDATVRSLVVGLPIIAISVDYRLAPAHPFPAAIDDAFVVASAVLDGVSGLSIDTSRVAIGGDSAGGNIAAVTAQQLRERA
VDSVSVSGLATALARQVLIFPVTDVSTTDTPSYLTFGKDCYLTKDAMERYIEQYADGHDRTDPRLSPLLASDLSGLPPTT
IVYGECDVLAHEVRAYGQALLEAGNSVTITEFMGQIHAFINLGGISSDARAARRLIRAELEAALC

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=336, Percent_Identity=25.8928571428571, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI68051721, Length=202, Percent_Identity=31.6831683168317, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI206597554, Length=211, Percent_Identity=27.0142180094787, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157041239, Length=317, Percent_Identity=25.2365930599369, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI226423947, Length=210, Percent_Identity=30.4761904761905, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786682, Length=250, Percent_Identity=28.8, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17567059, Length=274, Percent_Identity=30.2919708029197, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71996133, Length=152, Percent_Identity=34.8684210526316, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17540028, Length=165, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=160, Percent_Identity=31.25, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 32009; Mature: 32009

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLHPDAQFYIDTLSTLSAEEQVNFGKDAVGSGVDATHSAADQDIAGVPVRVYTPLSGAG
CCCCCCHHHHHHHHHHHCHHHHCCCCCHHCCCCCCCHHHCCCCCCCCCCEEEEECCCCCC
DFPCLVYFHGGGWSGGTLDMIDATVRSLVVGLPIIAISVDYRLAPAHPFPAAIDDAFVVA
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECEEECCCCCCCCHHHHHHHHH
SAVLDGVSGLSIDTSRVAIGGDSAGGNIAAVTAQQLRERAVDSVSVSGLATALARQVLIF
HHHHHCCCCCEECCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
PVTDVSTTDTPSYLTFGKDCYLTKDAMERYIEQYADGHDRTDPRLSPLLASDLSGLPPTT
EECCCCCCCCCCCEEECCCEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCE
IVYGECDVLAHEVRAYGQALLEAGNSVTITEFMGQIHAFINLGGISSDARAARRLIRAEL
EEECCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
EAALC
HHHCC
>Mature Secondary Structure
MFLHPDAQFYIDTLSTLSAEEQVNFGKDAVGSGVDATHSAADQDIAGVPVRVYTPLSGAG
CCCCCCHHHHHHHHHHHCHHHHCCCCCHHCCCCCCCHHHCCCCCCCCCCEEEEECCCCCC
DFPCLVYFHGGGWSGGTLDMIDATVRSLVVGLPIIAISVDYRLAPAHPFPAAIDDAFVVA
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECEEECCCCCCCCHHHHHHHHH
SAVLDGVSGLSIDTSRVAIGGDSAGGNIAAVTAQQLRERAVDSVSVSGLATALARQVLIF
HHHHHCCCCCEECCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
PVTDVSTTDTPSYLTFGKDCYLTKDAMERYIEQYADGHDRTDPRLSPLLASDLSGLPPTT
EECCCCCCCCCCCEEECCCEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCE
IVYGECDVLAHEVRAYGQALLEAGNSVTITEFMGQIHAFINLGGISSDARAARRLIRAEL
EEECCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
EAALC
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]