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The map label for this gene is pinE [H]

Identifier: 145294202

GI number: 145294202

Start: 175421

End: 176038

Strand: Reverse

Name: pinE [H]

Synonym: cgR_0159

Alternate gene names: 145294202

Gene position: 176038-175421 (Counterclockwise)

Preceding gene: 145294203

Following gene: 145294201

Centisome position: 5.31

GC content: 65.05

Gene sequence:

>618_bases
ATGGCACTGATTGGGTATATGCGGGTCTCGAAGTCGGATGGATCGCAGACGACGGATCTGCAACGCGACGCCCTCATCGC
GGCCGGGGTGGATCCGGGGCATCTCTACGAGGACCGGGCGTCAGGCAAGCAAGAAGACCGCCCACACCTGGATGCCTGCC
TCAAGGCACTGCGTCCCGGGGACACCCTGCTGGTGTGGAAGCTCGACCGGCTGGGCAGGAATCTGTGGCACCTGGTCAAC
ATCGTCCACGATCTCACCGCCCGCGAAGTAGGACTCAAAGTTCTCACCGGGCAGGGGGCGGCAATCGATACCACCTCGGC
CCAGGGCAAGCTCGTGTTCGGGATCTTCGCCGCCCTGGCCGAGTTCGAACGCGAGTTGATCTCGGAGCGGACGAAGGCCG
GCCTGGAGTCGGCGCGGGCCCGCGGGCGCAAGGGTGGTCGGCCGTTCAAGATGACACCGGCGAAAGTGCGGTTGGCGATG
GCGGCAATGGGCCAACCCGAAACCAGCGTCGCCGCGTTGTGTAAAGAACTCGGGATTACCCGTCAGACCCTCTACCGGCA
CGTCTCTCCCACCGGTGAGCTGCGGGAGGATGGGCGCAAGCTGCTTTCCGGGAGCTGA

Upstream 100 bases:

>100_bases
GCTCACCACATCGTGTTTGTGCAATCGCCTTCTGACCCTGACAACGATATTTTTTCGATGCGTGTCACGTAAATGATCGT
ATAAGTGATGGGAGAACACT

Downstream 100 bases:

>100_bases
CTACTGGCGGTAGAGCGCGGGTGGATCGGGTTTTCTGGGGTCGTCTCGAGGTGTCTTATATATAGAGGTATAGGGGAGAA
AAATTGAGGGGGAGAATCCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MALIGYMRVSKSDGSQTTDLQRDALIAAGVDPGHLYEDRASGKQEDRPHLDACLKALRPGDTLLVWKLDRLGRNLWHLVN
IVHDLTAREVGLKVLTGQGAAIDTTSAQGKLVFGIFAALAEFERELISERTKAGLESARARGRKGGRPFKMTPAKVRLAM
AAMGQPETSVAALCKELGITRQTLYRHVSPTGELREDGRKLLSGS

Sequences:

>Translated_205_residues
MALIGYMRVSKSDGSQTTDLQRDALIAAGVDPGHLYEDRASGKQEDRPHLDACLKALRPGDTLLVWKLDRLGRNLWHLVN
IVHDLTAREVGLKVLTGQGAAIDTTSAQGKLVFGIFAALAEFERELISERTKAGLESARARGRKGGRPFKMTPAKVRLAM
AAMGQPETSVAALCKELGITRQTLYRHVSPTGELREDGRKLLSGS
>Mature_204_residues
ALIGYMRVSKSDGSQTTDLQRDALIAAGVDPGHLYEDRASGKQEDRPHLDACLKALRPGDTLLVWKLDRLGRNLWHLVNI
VHDLTAREVGLKVLTGQGAAIDTTSAQGKLVFGIFAALAEFERELISERTKAGLESARARGRKGGRPFKMTPAKVRLAMA
AMGQPETSVAALCKELGITRQTLYRHVSPTGELREDGRKLLSGS

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=193, Percent_Identity=48.1865284974093, Blast_Score=154, Evalue=3e-39,
Organism=Escherichia coli, GI1787638, Length=188, Percent_Identity=35.1063829787234, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787827, Length=188, Percent_Identity=35.6382978723404, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22301; Mature: 22170

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIGYMRVSKSDGSQTTDLQRDALIAAGVDPGHLYEDRASGKQEDRPHLDACLKALRPG
CCEEEEEEECCCCCCCCHHHHHHHEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCC
DTLLVWKLDRLGRNLWHLVNIVHDLTAREVGLKVLTGQGAAIDTTSAQGKLVFGIFAALA
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEECCCCCCHHHHHHHHHHH
EFERELISERTKAGLESARARGRKGGRPFKMTPAKVRLAMAAMGQPETSVAALCKELGIT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCH
RQTLYRHVSPTGELREDGRKLLSGS
HHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALIGYMRVSKSDGSQTTDLQRDALIAAGVDPGHLYEDRASGKQEDRPHLDACLKALRPG
CEEEEEEECCCCCCCCHHHHHHHEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCC
DTLLVWKLDRLGRNLWHLVNIVHDLTAREVGLKVLTGQGAAIDTTSAQGKLVFGIFAALA
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEECCCCCCHHHHHHHHHHH
EFERELISERTKAGLESARARGRKGGRPFKMTPAKVRLAMAAMGQPETSVAALCKELGIT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCH
RQTLYRHVSPTGELREDGRKLLSGS
HHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]