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The map label for this gene is shiA [H]

Identifier: 145294104

GI number: 145294104

Start: 72209

End: 73549

Strand: Reverse

Name: shiA [H]

Synonym: cgR_0062

Alternate gene names: 145294104

Gene position: 73549-72209 (Counterclockwise)

Preceding gene: 145294105

Following gene: 145294103

Centisome position: 2.22

GC content: 54.06

Gene sequence:

>1341_bases
ATGGCATCTTTCCTTGGCTCCACACTTGAGTACTACGATTTCTTCATTTACGGCACCGCAGCAGCACTCGTCTTCCCTAA
GCTCTTCTTCCCGGATGGAAACCCTGCGATCGCTACTATCGCGGCTTTTGCAACATTCGGTGTTGCTTATATCGCTCGTC
CTTTAGGAGGCCTTGTCATGGGACACTTCGGTGACCGCTTGGGCCGAAAGAATGTCCTCCTTGTCACCTTGCTCATCATG
GGTATCGCTTCACTATCCATCGGTTTGCTACCCACATATTCGCAGATCGGTATTTGGGCTACAATCCTCCTCGTTGTCGC
CCGTCTAGCTCAAGGTTTCTCAGCGGGTGCCGAAGCAGCCGGCGCATCGTCTCTTACCTTGGAACACTCACCGGAAGGTC
GACGTGGTTTCTTCAATTCCTTCGTGATGACCGGTTACGCATCCGGAATGGTGTTGTCGACCTTGGTTTTCATCCCTGTT
GCAGCGCTCCCTGAAGCACAAATGTTGAGTTGGGGCTGGCGAATCCCCTTCCTGCTTTCAGTCGTAGTCCTCATCATTGC
CTTCTGGGTCCGAACGAGACTCGATGAAACTCCGGTCTTTGAAGATGAAGTTGCTGAAGCAGGGGAAGAGAAGAAACTCC
CTGCTGGTCGAGTTTTGAAGTACCAGGGTGGCGACGTTTTGCGAGTGCTGCTGATGTCCATCTTCTCAGTCATGCAGACC
ATCTTCACGGTTTTCGGATTGTCCTACGCAACCAGCCACGGTGGCTTCGAACGTTCCTCCATCCTTGCAGTAAACGCCAT
CGCCATCGGTCTATCTATGATTATGATGCCGCTAGCTGGACGACTCTCTGACCGCATCGGCCGCAAGCCACTCATGCTGA
CTGCCATGATCGGCTGTGGATTCACCATCTTCGTCTACTTCTTGGCCCTTGGTACTGGAAACATCCTGCTCGTCTTCGGC
GCAAGCTTCCTGTTCATGACCATCCTTTACTCCGGATTCAATGGCGTCTGGACCAGCTTCTTCGCTGAACTCTTCGCAGC
GCCAGTGCGCTACACCGGCATGGCCATGGGCAATCAGCTCGGCCTGGTCGTCGCAGGTTTCGGCCCTATGATCGGCGGCA
TGTTGCTTAACGACGGCACCTACGGCTGGATTCCAGTCGCCGTATTCGGTGCAGGTTGCTCTTTGCTGGCAATCGGCGCA
GTGCTCTCCACACGTGAAACCGCATTCACCGATATCAAACTGCTCGGTGAGCCTTATTTGAAGGCAACCGGCGCATGGCC
AACACAGCAAGCAGCTCAGCCTGCAATGTCCCAGACCAAAGAACCAGCACCCAGCTTGTAA

Upstream 100 bases:

>100_bases
CTCCCCTGATTTCCATCAAGAAAAGGACCACATCATGTCTACACACAGCGCAACACTTAGTTCAGATCTGGAAGCTAGAC
GCAGTCCCAAGAAAGCTGCC

Downstream 100 bases:

>100_bases
TCAGAAACTCACCACAAAGAGAGAAGCATCCCTTGAGTAAGTCCTCTACCTCCATCAAGACGATCACCACCGACCGAACC
TGCGATGTCCTTGTCATCGG

Product: hypothetical protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MASFLGSTLEYYDFFIYGTAAALVFPKLFFPDGNPAIATIAAFATFGVAYIARPLGGLVMGHFGDRLGRKNVLLVTLLIM
GIASLSIGLLPTYSQIGIWATILLVVARLAQGFSAGAEAAGASSLTLEHSPEGRRGFFNSFVMTGYASGMVLSTLVFIPV
AALPEAQMLSWGWRIPFLLSVVVLIIAFWVRTRLDETPVFEDEVAEAGEEKKLPAGRVLKYQGGDVLRVLLMSIFSVMQT
IFTVFGLSYATSHGGFERSSILAVNAIAIGLSMIMMPLAGRLSDRIGRKPLMLTAMIGCGFTIFVYFLALGTGNILLVFG
ASFLFMTILYSGFNGVWTSFFAELFAAPVRYTGMAMGNQLGLVVAGFGPMIGGMLLNDGTYGWIPVAVFGAGCSLLAIGA
VLSTRETAFTDIKLLGEPYLKATGAWPTQQAAQPAMSQTKEPAPSL

Sequences:

>Translated_446_residues
MASFLGSTLEYYDFFIYGTAAALVFPKLFFPDGNPAIATIAAFATFGVAYIARPLGGLVMGHFGDRLGRKNVLLVTLLIM
GIASLSIGLLPTYSQIGIWATILLVVARLAQGFSAGAEAAGASSLTLEHSPEGRRGFFNSFVMTGYASGMVLSTLVFIPV
AALPEAQMLSWGWRIPFLLSVVVLIIAFWVRTRLDETPVFEDEVAEAGEEKKLPAGRVLKYQGGDVLRVLLMSIFSVMQT
IFTVFGLSYATSHGGFERSSILAVNAIAIGLSMIMMPLAGRLSDRIGRKPLMLTAMIGCGFTIFVYFLALGTGNILLVFG
ASFLFMTILYSGFNGVWTSFFAELFAAPVRYTGMAMGNQLGLVVAGFGPMIGGMLLNDGTYGWIPVAVFGAGCSLLAIGA
VLSTRETAFTDIKLLGEPYLKATGAWPTQQAAQPAMSQTKEPAPSL
>Mature_445_residues
ASFLGSTLEYYDFFIYGTAAALVFPKLFFPDGNPAIATIAAFATFGVAYIARPLGGLVMGHFGDRLGRKNVLLVTLLIMG
IASLSIGLLPTYSQIGIWATILLVVARLAQGFSAGAEAAGASSLTLEHSPEGRRGFFNSFVMTGYASGMVLSTLVFIPVA
ALPEAQMLSWGWRIPFLLSVVVLIIAFWVRTRLDETPVFEDEVAEAGEEKKLPAGRVLKYQGGDVLRVLLMSIFSVMQTI
FTVFGLSYATSHGGFERSSILAVNAIAIGLSMIMMPLAGRLSDRIGRKPLMLTAMIGCGFTIFVYFLALGTGNILLVFGA
SFLFMTILYSGFNGVWTSFFAELFAAPVRYTGMAMGNQLGLVVAGFGPMIGGMLLNDGTYGWIPVAVFGAGCSLLAIGAV
LSTRETAFTDIKLLGEPYLKATGAWPTQQAAQPAMSQTKEPAPSL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=407, Percent_Identity=39.5577395577396, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1790550, Length=395, Percent_Identity=35.4430379746835, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1789941, Length=415, Percent_Identity=37.3493975903614, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1788942, Length=363, Percent_Identity=32.2314049586777, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87082231, Length=207, Percent_Identity=26.5700483091787, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87082404, Length=373, Percent_Identity=21.71581769437, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47568; Mature: 47437

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCHHHHCHHHHHCCCCCCCC
>Mature Secondary Structure 
ASFLGSTLEYYDFFIYGTAAALVFPKLFFPDGNPAIATIAAFATFGVAYIARPLGGLVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GHFGDRLGRKNVLLVTLLIMGIASLSIGLLPTYSQIGIWATILLVVARLAQGFSAGAEAA
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GASSLTLEHSPEGRRGFFNSFVMTGYASGMVLSTLVFIPVAALPEAQMLSWGWRIPFLLS
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHH
VVVLIIAFWVRTRLDETPVFEDEVAEAGEEKKLPAGRVLKYQGGDVLRVLLMSIFSVMQT
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
IFTVFGLSYATSHGGFERSSILAVNAIAIGLSMIMMPLAGRLSDRIGRKPLMLTAMIGCG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FTIFVYFLALGTGNILLVFGASFLFMTILYSGFNGVWTSFFAELFAAPVRYTGMAMGNQL
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GLVVAGFGPMIGGMLLNDGTYGWIPVAVFGAGCSLLAIGAVLSTRETAFTDIKLLGEPYL
CEEEECHHHHHCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCHH
KATGAWPTQQAAQPAMSQTKEPAPSL
HCCCCCCCHHHHCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]