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The map label for this gene is kstD [H]

Identifier: 145294103

GI number: 145294103

Start: 70422

End: 72176

Strand: Reverse

Name: kstD [H]

Synonym: cgR_0061

Alternate gene names: 145294103

Gene position: 72176-70422 (Counterclockwise)

Preceding gene: 145294104

Following gene: 145294102

Centisome position: 2.18

GC content: 53.33

Gene sequence:

>1755_bases
TTGAGTAAGTCCTCTACCTCCATCAAGACGATCACCACCGACCGAACCTGCGATGTCCTTGTCATCGGATCAGGAGCATC
TGGCCTTGCCACCGCACTTGCCGCAGCCCATCAGGGACTTGATGTCATCGTCGCAGAGAAAGAAAAGTACTTTGGCGGCA
CCACCGCAATTTCTGCTGGTTGGGCATGGGTTCCCGGAAACCCCAAGGGCGCTGAAGCCACTGGCGATAGCCGTGAAGAA
GTAGAAACCTACCTCAAAGCATTAGCACCGGAGCATTACAACGAAGCTGGTGTCAGCGCATTTCTGGATACCGTCCCAGA
AGCCATCAGCTTCTTTGAAAACAACACCGAGGTGAACTTTGTCTATCCAGAAAAAGCACCCGACTACCAAATGGATCTTC
CCGGTGCTCGCCTAAGTGGCCGTGCAATCCTTCCCGATGATGCCGATGCACGCATGCTCGGCGATCGTCGTCGAGAACTA
CAACCATATTTGAGCAGTTACACCGTTTTTGGATACATGCCCCAGGTCGGCGCAGACCTCAACCAAGTCCTCCACGCCAA
CCGTTCCATCAAATCTTTCCTCTACGTTGCAACAAAACTCACTCGCACTTGGTTTGATACAGCCATCCACGGTCAACCGC
TCAAACGAACCAACGGCTCCGCATTAATCACCCGCATGGTGAAATCAGCACTCGACGCTGGAGTTCAGATCTGGACTTCC
ACACCTGTCGAGTCCCTCATTCAGGAATCTGACGGTTCAGTTTCCGGCGCGATCCTTGGTGGCGTTCACTCCGGAAAGGT
AAAAGCCCGGATGGGTGTTGTGCTGGCAGGTGGAGGATTTACCGGAAACACTTCCCTACGCAGTCAATACTTCCCCCACG
ATTCACACGGCACCAACCACATCACCCCAACAGTTGGCCATGATGGATCAAGCGCCGAAATGGCGATACAAGCAGGAGGC
CGCATCAACGATGACCTCTTCGCACCAGGTTCTTGGGCACCGATTACCGCATTCAAACAGCTTCGCGATGGCCGCCAGCG
CCTCTTCCCACACCTTCGCCAAATCGGCCTACCCGGCATGATTACCGTCGACCGCACAGGAAAACGCTTCGGAAATGAAG
CCCTAAGCTACCACGATTTTGGGCGTCAGCTTCTCGAGCACGACAAAAACCATGACGATACCTACGCATGGATCATCGCC
GACGAAGCTTGCATGCATACCTACGGAATTGGCTACGCAAAACCGTGGCCCATCCCCCGCAAGTACTTCCATGACATCGG
ATTCCTCCACAAAGCAAATACCCTCCGCGATTTAGCCATCAAAATTGGCGCAGACCCCGCAACTCTTGAAGCCACTGTCA
ACCGCTTCAATGGATTTGCGGAAAAGGGAGTGGATGATGACTTTGGTCGAGGCTCCACCGCTTACAACCACTTCCGTGGC
GATCCCGAGCATAAACCAAACCCCAACCTAGCCACCCTGGAAAAAGCTCCTTATTATGCAGTCAAGATTCAGATGGGTGA
TCTAGGATCCTTCGCTGGAATCGATGTTGATGATCACTCAACCGTCATTGATACAGCAGGAGAACCAATTCGCGGTCTCT
ACTCTGTCGGCGCCTCTGCAGTCTCGGTATTCGGTGGTGGCTATCCAGGCTATGGCTCGCACATCGGACCAGCCCTTGTG
TTTGGCTACCGAATGGGTCGCGACATTGCGAAACTATCTCAAGAGACATCTACTGTGAAAGCAGAGTCTGCCTAA

Upstream 100 bases:

>100_bases
CATGGCCAACACAGCAAGCAGCTCAGCCTGCAATGTCCCAGACCAAAGAACCAGCACCCAGCTTGTAATCAGAAACTCAC
CACAAAGAGAGAAGCATCCC

Downstream 100 bases:

>100_bases
AGCTACGAAGGTGAATTGACATCGAGGTAATCATCGGCAAGGTATTGACCCAACTTGTCTAGTACTGCCGATCGATTAGC
CTCGCTTGCGCGACTTTCTG

Product: hypothetical protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MSKSSTSIKTITTDRTCDVLVIGSGASGLATALAAAHQGLDVIVAEKEKYFGGTTAISAGWAWVPGNPKGAEATGDSREE
VETYLKALAPEHYNEAGVSAFLDTVPEAISFFENNTEVNFVYPEKAPDYQMDLPGARLSGRAILPDDADARMLGDRRREL
QPYLSSYTVFGYMPQVGADLNQVLHANRSIKSFLYVATKLTRTWFDTAIHGQPLKRTNGSALITRMVKSALDAGVQIWTS
TPVESLIQESDGSVSGAILGGVHSGKVKARMGVVLAGGGFTGNTSLRSQYFPHDSHGTNHITPTVGHDGSSAEMAIQAGG
RINDDLFAPGSWAPITAFKQLRDGRQRLFPHLRQIGLPGMITVDRTGKRFGNEALSYHDFGRQLLEHDKNHDDTYAWIIA
DEACMHTYGIGYAKPWPIPRKYFHDIGFLHKANTLRDLAIKIGADPATLEATVNRFNGFAEKGVDDDFGRGSTAYNHFRG
DPEHKPNPNLATLEKAPYYAVKIQMGDLGSFAGIDVDDHSTVIDTAGEPIRGLYSVGASAVSVFGGGYPGYGSHIGPALV
FGYRMGRDIAKLSQETSTVKAESA

Sequences:

>Translated_584_residues
MSKSSTSIKTITTDRTCDVLVIGSGASGLATALAAAHQGLDVIVAEKEKYFGGTTAISAGWAWVPGNPKGAEATGDSREE
VETYLKALAPEHYNEAGVSAFLDTVPEAISFFENNTEVNFVYPEKAPDYQMDLPGARLSGRAILPDDADARMLGDRRREL
QPYLSSYTVFGYMPQVGADLNQVLHANRSIKSFLYVATKLTRTWFDTAIHGQPLKRTNGSALITRMVKSALDAGVQIWTS
TPVESLIQESDGSVSGAILGGVHSGKVKARMGVVLAGGGFTGNTSLRSQYFPHDSHGTNHITPTVGHDGSSAEMAIQAGG
RINDDLFAPGSWAPITAFKQLRDGRQRLFPHLRQIGLPGMITVDRTGKRFGNEALSYHDFGRQLLEHDKNHDDTYAWIIA
DEACMHTYGIGYAKPWPIPRKYFHDIGFLHKANTLRDLAIKIGADPATLEATVNRFNGFAEKGVDDDFGRGSTAYNHFRG
DPEHKPNPNLATLEKAPYYAVKIQMGDLGSFAGIDVDDHSTVIDTAGEPIRGLYSVGASAVSVFGGGYPGYGSHIGPALV
FGYRMGRDIAKLSQETSTVKAESA
>Mature_583_residues
SKSSTSIKTITTDRTCDVLVIGSGASGLATALAAAHQGLDVIVAEKEKYFGGTTAISAGWAWVPGNPKGAEATGDSREEV
ETYLKALAPEHYNEAGVSAFLDTVPEAISFFENNTEVNFVYPEKAPDYQMDLPGARLSGRAILPDDADARMLGDRRRELQ
PYLSSYTVFGYMPQVGADLNQVLHANRSIKSFLYVATKLTRTWFDTAIHGQPLKRTNGSALITRMVKSALDAGVQIWTST
PVESLIQESDGSVSGAILGGVHSGKVKARMGVVLAGGGFTGNTSLRSQYFPHDSHGTNHITPTVGHDGSSAEMAIQAGGR
INDDLFAPGSWAPITAFKQLRDGRQRLFPHLRQIGLPGMITVDRTGKRFGNEALSYHDFGRQLLEHDKNHDDTYAWIIAD
EACMHTYGIGYAKPWPIPRKYFHDIGFLHKANTLRDLAIKIGADPATLEATVNRFNGFAEKGVDDDFGRGSTAYNHFRGD
PEHKPNPNLATLEKAPYYAVKIQMGDLGSFAGIDVDDHSTVIDTAGEPIRGLYSVGASAVSVFGGGYPGYGSHIGPALVF
GYRMGRDIAKLSQETSTVKAESA

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 62993; Mature: 62862

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSSTSIKTITTDRTCDVLVIGSGASGLATALAAAHQGLDVIVAEKEKYFGGTTAISAG
CCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCHHHCCCCEEECCC
WAWVPGNPKGAEATGDSREEVETYLKALAPEHYNEAGVSAFLDTVPEAISFFENNTEVNF
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
VYPEKAPDYQMDLPGARLSGRAILPDDADARMLGDRRRELQPYLSSYTVFGYMPQVGADL
EECCCCCCCEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHEEEEECCCHHCCHH
NQVLHANRSIKSFLYVATKLTRTWFDTAIHGQPLKRTNGSALITRMVKSALDAGVQIWTS
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEC
TPVESLIQESDGSVSGAILGGVHSGKVKARMGVVLAGGGFTGNTSLRSQYFPHDSHGTNH
CCHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCHHHCCCCCCCCCCCC
ITPTVGHDGSSAEMAIQAGGRINDDLFAPGSWAPITAFKQLRDGRQRLFPHLRQIGLPGM
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
ITVDRTGKRFGNEALSYHDFGRQLLEHDKNHDDTYAWIIADEACMHTYGIGYAKPWPIPR
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCCH
KYFHDIGFLHKANTLRDLAIKIGADPATLEATVNRFNGFAEKGVDDDFGRGSTAYNHFRG
HHHHHHHHHHHCCHHEEEHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCC
DPEHKPNPNLATLEKAPYYAVKIQMGDLGSFAGIDVDDHSTVIDTAGEPIRGLYSVGASA
CCCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCHHHHHHHCHHH
VSVFGGGYPGYGSHIGPALVFGYRMGRDIAKLSQETSTVKAESA
HHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCEECCCC
>Mature Secondary Structure 
SKSSTSIKTITTDRTCDVLVIGSGASGLATALAAAHQGLDVIVAEKEKYFGGTTAISAG
CCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCHHHCCCCEEECCC
WAWVPGNPKGAEATGDSREEVETYLKALAPEHYNEAGVSAFLDTVPEAISFFENNTEVNF
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
VYPEKAPDYQMDLPGARLSGRAILPDDADARMLGDRRRELQPYLSSYTVFGYMPQVGADL
EECCCCCCCEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHEEEEECCCHHCCHH
NQVLHANRSIKSFLYVATKLTRTWFDTAIHGQPLKRTNGSALITRMVKSALDAGVQIWTS
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEC
TPVESLIQESDGSVSGAILGGVHSGKVKARMGVVLAGGGFTGNTSLRSQYFPHDSHGTNH
CCHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCHHHCCCCCCCCCCCC
ITPTVGHDGSSAEMAIQAGGRINDDLFAPGSWAPITAFKQLRDGRQRLFPHLRQIGLPGM
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
ITVDRTGKRFGNEALSYHDFGRQLLEHDKNHDDTYAWIIADEACMHTYGIGYAKPWPIPR
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCCH
KYFHDIGFLHKANTLRDLAIKIGADPATLEATVNRFNGFAEKGVDDDFGRGSTAYNHFRG
HHHHHHHHHHHCCHHEEEHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCC
DPEHKPNPNLATLEKAPYYAVKIQMGDLGSFAGIDVDDHSTVIDTAGEPIRGLYSVGASA
CCCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCCCHHHHHHHCHHH
VSVFGGGYPGYGSHIGPALVFGYRMGRDIAKLSQETSTVKAESA
HHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]