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The map label for this gene is hisJ [H]

Identifier: 138897014

GI number: 138897014

Start: 3492917

End: 3493738

Strand: Direct

Name: hisJ [H]

Synonym: GTNG_3385

Alternate gene names: 138897014

Gene position: 3492917-3493738 (Clockwise)

Preceding gene: 138897012

Following gene: 138897015

Centisome position: 98.38

GC content: 43.07

Gene sequence:

>822_bases
ATGTTAAGATTGAAGAAAGGATTCATCGTGGCTGTCGTTGCTGCGTTGTTTATGGCGCTCGCCGCTTGCGGCAAGTCGAC
AGAAACAACCTCTTCGTCAGGCAGTGAGAAAGAAACGAAACAAAAAATCACTGTCGGAACAGACGCTGCGTTTGCTCCGT
TTGAGTATATGGAAAAAGGGAAAGTCGTTGGTTTTGATGCTGACCTTCTCGATGCGGTCATGAAAGAAGCCGGCTTGGAC
TATGAGTTGAAAAACATCGGCTGGGATCCGCTGTTCGCTTCGCTGCAAAGCAAAGAAATCGACATGGGCATTTCCGGTAT
TACTATCACGGATAAGCGGAAACAATCGTATGATTTCTCAGCTCCGTACTTTGAAGCAACCCAAGTGATCTTAGTGAAAG
AAGGCAGCCTAGTGAAAAACGCCCTCGACTTAAAAGGGAAAACGATCGGTGTCCAAAACGCGACCACTGGTCAAGAAGCT
GCTGAAAAGCTGTTTGGCAAAGGTGAGCATATTAAAAAGTTCGAAACAAGTGTTGTTGCCATTATGGAGATGTTAAACGG
CGGTGTTGATGCCGTGATCACCGATAACGCAGTAGCGAACGAATATGTGAAAAACAATCCGGACAAAAAATTGCAAGTCA
TTGAAGATCCGGACAACTTCACTTCGGAATATTACGGTATCATCTTCCCGAAAGATAGCGACTTGAAAGCAAAAGTGGAT
GAAGCGTTAAAGAACATCATTGAAAACGGTAAATATACAGAAGTTTACAAAAAATGGTTTGGCATAGAACCGAATATTGA
AAATTTAAAGCAACAACAATAA

Upstream 100 bases:

>100_bases
TTCAAACAATTATTTTTATAAAACCTATTGCATAATCACAAAAATATTGATAGTATGATAATCGTTCATCGACAATGATT
CCAAAGGGGGATTTTCTAGG

Downstream 100 bases:

>100_bases
GCGCACGAACAAAGGAAAATGCGTAACTGCTAGCAAGTTACGCATTTTTCTTAAGAGAAAGGTGAGATTGATGGATTTTC
GTTTTGATATTATTGTTGAA

Product: glutamine transport system substrate-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLRLKKGFIVAVVAALFMALAACGKSTETTSSSGSEKETKQKITVGTDAAFAPFEYMEKGKVVGFDADLLDAVMKEAGLD
YELKNIGWDPLFASLQSKEIDMGISGITITDKRKQSYDFSAPYFEATQVILVKEGSLVKNALDLKGKTIGVQNATTGQEA
AEKLFGKGEHIKKFETSVVAIMEMLNGGVDAVITDNAVANEYVKNNPDKKLQVIEDPDNFTSEYYGIIFPKDSDLKAKVD
EALKNIIENGKYTEVYKKWFGIEPNIENLKQQQ

Sequences:

>Translated_273_residues
MLRLKKGFIVAVVAALFMALAACGKSTETTSSSGSEKETKQKITVGTDAAFAPFEYMEKGKVVGFDADLLDAVMKEAGLD
YELKNIGWDPLFASLQSKEIDMGISGITITDKRKQSYDFSAPYFEATQVILVKEGSLVKNALDLKGKTIGVQNATTGQEA
AEKLFGKGEHIKKFETSVVAIMEMLNGGVDAVITDNAVANEYVKNNPDKKLQVIEDPDNFTSEYYGIIFPKDSDLKAKVD
EALKNIIENGKYTEVYKKWFGIEPNIENLKQQQ
>Mature_273_residues
MLRLKKGFIVAVVAALFMALAACGKSTETTSSSGSEKETKQKITVGTDAAFAPFEYMEKGKVVGFDADLLDAVMKEAGLD
YELKNIGWDPLFASLQSKEIDMGISGITITDKRKQSYDFSAPYFEATQVILVKEGSLVKNALDLKGKTIGVQNATTGQEA
AEKLFGKGEHIKKFETSVVAIMEMLNGGVDAVITDNAVANEYVKNNPDKKLQVIEDPDNFTSEYYGIIFPKDSDLKAKVD
EALKNIIENGKYTEVYKKWFGIEPNIENLKQQQ

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=254, Percent_Identity=38.5826771653543, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787085, Length=223, Percent_Identity=31.8385650224215, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788228, Length=259, Percent_Identity=30.8880308880309, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1787088, Length=264, Percent_Identity=29.1666666666667, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788649, Length=239, Percent_Identity=29.2887029288703, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30039; Mature: 30039

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLKKGFIVAVVAALFMALAACGKSTETTSSSGSEKETKQKITVGTDAAFAPFEYMEKG
CCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCHHHHHCC
KVVGFDADLLDAVMKEAGLDYELKNIGWDPLFASLQSKEIDMGISGITITDKRKQSYDFS
CEEEECHHHHHHHHHHCCCCEEHHHCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCC
APYFEATQVILVKEGSLVKNALDLKGKTIGVQNATTGQEAAEKLFGKGEHIKKFETSVVA
CCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
IMEMLNGGVDAVITDNAVANEYVKNNPDKKLQVIEDPDNFTSEYYGIIFPKDSDLKAKVD
HHHHHCCCCCEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHH
EALKNIIENGKYTEVYKKWFGIEPNIENLKQQQ
HHHHHHHCCCCHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MLRLKKGFIVAVVAALFMALAACGKSTETTSSSGSEKETKQKITVGTDAAFAPFEYMEKG
CCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCHHHHHCC
KVVGFDADLLDAVMKEAGLDYELKNIGWDPLFASLQSKEIDMGISGITITDKRKQSYDFS
CEEEECHHHHHHHHHHCCCCEEHHHCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCC
APYFEATQVILVKEGSLVKNALDLKGKTIGVQNATTGQEAAEKLFGKGEHIKKFETSVVA
CCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
IMEMLNGGVDAVITDNAVANEYVKNNPDKKLQVIEDPDNFTSEYYGIIFPKDSDLKAKVD
HHHHHCCCCCEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHH
EALKNIIENGKYTEVYKKWFGIEPNIENLKQQQ
HHHHHHHCCCCHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]