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The map label for this gene is sigB [H]

Identifier: 138897001

GI number: 138897001

Start: 3476792

End: 3477568

Strand: Direct

Name: sigB [H]

Synonym: GTNG_3372

Alternate gene names: 138897001

Gene position: 3476792-3477568 (Clockwise)

Preceding gene: 138897000

Following gene: 138897009

Centisome position: 97.93

GC content: 52.12

Gene sequence:

>777_bases
ATGCCCATGAATGTATATCCCCCTCCTCTGTCCAAGAGCAAGCTTGACGCCCTCATTTCCGCCTATCAACAAACAAAGAC
GGAAGAAGCGGCGACAGCACTGCTCGTTCGCTTCGAGCCGCTCATTGCGGCCGCAGCAAAAAAAATGGCGCGCAGCCGTC
CTGATTTTTATGAGGACTTATTTCAAGTCGGACGGTTATCGTTTTTGCGCCTCCTTGAGCATTACGACCCGAACCAAGGA
ACAAGCTTTGAATCGTATGCAATGAAAAGCTTGATCGGCTATATGAAAAACTATTTGCGCGACAAAGCGTGGTATATTCA
AGTACCGCGCAGAGTGAAAGAAAACGGAAGCAAAGTGCAGAAGGCGATCGATGAGCTGACTGTCACACTTGAGCGATCAC
CGAACATCGAGGAAATAGCCAACCATTTAGGTTTATCGGTCGAAGAAACGATTGAAATTTTGGCTGGCCGCGACCATTAT
CAGGCGATTTCCATCGATGCCCCCGTGCAAGACGGGGAAAAGGACGCGACGACCATCGGCGAATTTCTTGCCGATGACAC
GAATGAGGTCGAGGCGCTGATCGAGCGGCTTGACTTGCAAGAAGCCATCGGCAAGCTGAGCGAGCAGGAGCGGATCGTCA
TCGAGGCGGTGTTTCGCCGCGGAGAAACACAGCGCTCGCTCGCTAAACGGCTCGGCGTCTCCCAAATGACGATCAGCCGC
ATCCAAAAGCGGGCGATTGAAAAACTGAAGCGGCAGCTTGCCGCCTATTCGTCATAA

Upstream 100 bases:

>100_bases
CTCTTTTTAATGGAAGCGCTCATGGACGATGTAAGTGTCACAACAGCGGACGGAACAGAAGTCCGCCTCACGAAATTCCG
CCATCGCGGCGAAGGAGAGC

Downstream 100 bases:

>100_bases
TGGCAGAAGAAAGCGCCGCCTTCACCATCAAGCAGTATAAACGCTGATGTCAACGGCGGCGCAATAGCAACAACGTCTGA
TCGTCTTTTTGTTCGTATTG

Product: sigma-B general stress transcription factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPMNVYPPPLSKSKLDALISAYQQTKTEEAATALLVRFEPLIAAAAKKMARSRPDFYEDLFQVGRLSFLRLLEHYDPNQG
TSFESYAMKSLIGYMKNYLRDKAWYIQVPRRVKENGSKVQKAIDELTVTLERSPNIEEIANHLGLSVEETIEILAGRDHY
QAISIDAPVQDGEKDATTIGEFLADDTNEVEALIERLDLQEAIGKLSEQERIVIEAVFRRGETQRSLAKRLGVSQMTISR
IQKRAIEKLKRQLAAYSS

Sequences:

>Translated_258_residues
MPMNVYPPPLSKSKLDALISAYQQTKTEEAATALLVRFEPLIAAAAKKMARSRPDFYEDLFQVGRLSFLRLLEHYDPNQG
TSFESYAMKSLIGYMKNYLRDKAWYIQVPRRVKENGSKVQKAIDELTVTLERSPNIEEIANHLGLSVEETIEILAGRDHY
QAISIDAPVQDGEKDATTIGEFLADDTNEVEALIERLDLQEAIGKLSEQERIVIEAVFRRGETQRSLAKRLGVSQMTISR
IQKRAIEKLKRQLAAYSS
>Mature_257_residues
PMNVYPPPLSKSKLDALISAYQQTKTEEAATALLVRFEPLIAAAAKKMARSRPDFYEDLFQVGRLSFLRLLEHYDPNQGT
SFESYAMKSLIGYMKNYLRDKAWYIQVPRRVKENGSKVQKAIDELTVTLERSPNIEEIANHLGLSVEETIEILAGRDHYQ
AISIDAPVQDGEKDATTIGEFLADDTNEVEALIERLDLQEAIGKLSEQERIVIEAVFRRGETQRSLAKRLGVSQMTISRI
QKRAIEKLKRQLAAYSS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=230, Percent_Identity=29.1304347826087, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29250; Mature: 29119

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMNVYPPPLSKSKLDALISAYQQTKTEEAATALLVRFEPLIAAAAKKMARSRPDFYEDL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FQVGRLSFLRLLEHYDPNQGTSFESYAMKSLIGYMKNYLRDKAWYIQVPRRVKENGSKVQ
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHH
KAIDELTVTLERSPNIEEIANHLGLSVEETIEILAGRDHYQAISIDAPVQDGEKDATTIG
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHH
EFLADDTNEVEALIERLDLQEAIGKLSEQERIVIEAVFRRGETQRSLAKRLGVSQMTISR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHH
IQKRAIEKLKRQLAAYSS
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PMNVYPPPLSKSKLDALISAYQQTKTEEAATALLVRFEPLIAAAAKKMARSRPDFYEDL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FQVGRLSFLRLLEHYDPNQGTSFESYAMKSLIGYMKNYLRDKAWYIQVPRRVKENGSKVQ
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHHHHHH
KAIDELTVTLERSPNIEEIANHLGLSVEETIEILAGRDHYQAISIDAPVQDGEKDATTIG
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHH
EFLADDTNEVEALIERLDLQEAIGKLSEQERIVIEAVFRRGETQRSLAKRLGVSQMTISR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHH
IQKRAIEKLKRQLAAYSS
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]