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The map label for this gene is ywjE [H]

Identifier: 138896973

GI number: 138896973

Start: 3453192

End: 3454382

Strand: Direct

Name: ywjE [H]

Synonym: GTNG_3344

Alternate gene names: 138896973

Gene position: 3453192-3454382 (Clockwise)

Preceding gene: 138896964

Following gene: 138896980

Centisome position: 97.26

GC content: 50.71

Gene sequence:

>1191_bases
ATGGTCGTTATTGCTGTGATTGCCATTATATTCGTACTCATTTATCTCGATGACAAGCTTGGTCAGCTCGCCTTCCGGAA
CGGACGCAAAAAAGTCGTCTATCCAGAACGGAGAAGCGATATAGCGCTCTATGTCAATGGCAGGCATTTATTTGCCGACT
ATTTTGCCGAGCTGCACCGTGCTCGCGACCATATTCACATTCTGTTTTACATTATCAAAAGTGACGAAACGACCACGCCG
TTTTTCCAAATTTTAAAAGAAAAAGCAGCCGAAGGAGTGAAAGTACGGGTGTTGGTCGACTGGGTGGGTAGCCTCGGGTT
GCCGAAAGCGCTCATTCGTTCACTAAGGGCAAGCGGGGTGGAGTTCGCCTATACCCGCCCGCCGTGCTTTCCGTTTTTGA
TTTACCGCCTCAATCGCCGCAATCATCGAAAAATTACGGTCATCGATGGGCGGATCGGCTACATCGGCGGCTATAACATC
GGCCGCGAATATAATGGGAAAGACGCCAATTTCGGGGAATGGCGGGACTATCACCTAAAAATCAGCGGCGAAGGAGTATA
TGACTTGCAAGAACAGTTTTTGCATGATTGGGAAGAAGCGACAAGCAAGACGGTGGCGGACAAACACCGGTATTTCCCGG
AGCTTCCCGCTGGCGCCATTCGCCATCAATTGGTCGCTACCGATGGCTCCGCCCTTGAGGAACAATATATCCATGCCATC
CGCCATGCCAAAAAAGAAATTATGATCGGTACCCCATACTTTATCCCGAGCCGGCCGCTGTTTAACGAACTGATGCGTGC
ACTCAAGCGCGGGGTGCGCGTCACCGTGCTTGTGCCGCTGAAAGCGGATCATTTATTTGTGAGAGAAGCCGCCTATCCGT
ATTTTTATCGCATGCTGAAAGCCGGGGCGCGCGTTTACCGCTTTTACCAAGGGTTTTACCATGCTAAAACCATTGTGATC
GACGAAGAATGGTGTGATGTCGGCACAGCGAATTTTGACCGACGTAGTTTGTTCTTAAATGGTGAAATCAACTGCTACGT
GTTTGATCGCGCATTTACACGGCTCGTCAAACAGGCGATCGAACGCGATTTGACGCGCTCGGAGCCGCTGACGCTCGATT
TTTGGCGGAACCGTTCGCTCCTTGACCGCGGCAAAGAGTCGATTTCCCAGCTCATTTCCGCTTGGCTATAG

Upstream 100 bases:

>100_bases
TTCGGTAAGATGGGAGAGAATCGGCGGCTTCAGCTCTGCTATTTTTGTGCGCTGTAGGATACATTAATAAGTACTGAAGC
GACGCACAGGAGGGGAAGGC

Downstream 100 bases:

>100_bases
CACGTATGTTTTCGGCAGGGAGCAGATGGAAGAAACACCAAAGCAAAGGAAGAAACAAAAAATCGGCCGGCGCCCATGGC
GACCTCATATCGCCAAAACT

Product: cardiolipin synthetase domain-containing protein

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MVVIAVIAIIFVLIYLDDKLGQLAFRNGRKKVVYPERRSDIALYVNGRHLFADYFAELHRARDHIHILFYIIKSDETTTP
FFQILKEKAAEGVKVRVLVDWVGSLGLPKALIRSLRASGVEFAYTRPPCFPFLIYRLNRRNHRKITVIDGRIGYIGGYNI
GREYNGKDANFGEWRDYHLKISGEGVYDLQEQFLHDWEEATSKTVADKHRYFPELPAGAIRHQLVATDGSALEEQYIHAI
RHAKKEIMIGTPYFIPSRPLFNELMRALKRGVRVTVLVPLKADHLFVREAAYPYFYRMLKAGARVYRFYQGFYHAKTIVI
DEEWCDVGTANFDRRSLFLNGEINCYVFDRAFTRLVKQAIERDLTRSEPLTLDFWRNRSLLDRGKESISQLISAWL

Sequences:

>Translated_396_residues
MVVIAVIAIIFVLIYLDDKLGQLAFRNGRKKVVYPERRSDIALYVNGRHLFADYFAELHRARDHIHILFYIIKSDETTTP
FFQILKEKAAEGVKVRVLVDWVGSLGLPKALIRSLRASGVEFAYTRPPCFPFLIYRLNRRNHRKITVIDGRIGYIGGYNI
GREYNGKDANFGEWRDYHLKISGEGVYDLQEQFLHDWEEATSKTVADKHRYFPELPAGAIRHQLVATDGSALEEQYIHAI
RHAKKEIMIGTPYFIPSRPLFNELMRALKRGVRVTVLVPLKADHLFVREAAYPYFYRMLKAGARVYRFYQGFYHAKTIVI
DEEWCDVGTANFDRRSLFLNGEINCYVFDRAFTRLVKQAIERDLTRSEPLTLDFWRNRSLLDRGKESISQLISAWL
>Mature_396_residues
MVVIAVIAIIFVLIYLDDKLGQLAFRNGRKKVVYPERRSDIALYVNGRHLFADYFAELHRARDHIHILFYIIKSDETTTP
FFQILKEKAAEGVKVRVLVDWVGSLGLPKALIRSLRASGVEFAYTRPPCFPFLIYRLNRRNHRKITVIDGRIGYIGGYNI
GREYNGKDANFGEWRDYHLKISGEGVYDLQEQFLHDWEEATSKTVADKHRYFPELPAGAIRHQLVATDGSALEEQYIHAI
RHAKKEIMIGTPYFIPSRPLFNELMRALKRGVRVTVLVPLKADHLFVREAAYPYFYRMLKAGARVYRFYQGFYHAKTIVI
DEEWCDVGTANFDRRSLFLNGEINCYVFDRAFTRLVKQAIERDLTRSEPLTLDFWRNRSLLDRGKESISQLISAWL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=377, Percent_Identity=28.6472148541114, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787007, Length=332, Percent_Identity=28.9156626506024, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 46136; Mature: 46136

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVIAVIAIIFVLIYLDDKLGQLAFRNGRKKVVYPERRSDIALYVNGRHLFADYFAELHR
CHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHH
ARDHIHILFYIIKSDETTTPFFQILKEKAAEGVKVRVLVDWVGSLGLPKALIRSLRASGV
CCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCC
EFAYTRPPCFPFLIYRLNRRNHRKITVIDGRIGYIGGYNIGREYNGKDANFGEWRDYHLK
EEEECCCCHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCCCCEEEEEE
ISGEGVYDLQEQFLHDWEEATSKTVADKHRYFPELPAGAIRHQLVATDGSALEEQYIHAI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCHHHHHHHHHHH
RHAKKEIMIGTPYFIPSRPLFNELMRALKRGVRVTVLVPLKADHLFVREAAYPYFYRMLK
HHHHHHEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEHHHHCCHHHHHHHH
AGARVYRFYQGFYHAKTIVIDEEWCDVGTANFDRRSLFLNGEINCYVFDRAFTRLVKQAI
HHHHHHHHHHHHHHEEEEEEEHHHCCCCCCCCCCCEEEECCCEEEEEEHHHHHHHHHHHH
ERDLTRSEPLTLDFWRNRSLLDRGKESISQLISAWL
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVIAVIAIIFVLIYLDDKLGQLAFRNGRKKVVYPERRSDIALYVNGRHLFADYFAELHR
CHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHH
ARDHIHILFYIIKSDETTTPFFQILKEKAAEGVKVRVLVDWVGSLGLPKALIRSLRASGV
CCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCC
EFAYTRPPCFPFLIYRLNRRNHRKITVIDGRIGYIGGYNIGREYNGKDANFGEWRDYHLK
EEEECCCCHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCCCCEEEEEE
ISGEGVYDLQEQFLHDWEEATSKTVADKHRYFPELPAGAIRHQLVATDGSALEEQYIHAI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCHHHHHHHHHHH
RHAKKEIMIGTPYFIPSRPLFNELMRALKRGVRVTVLVPLKADHLFVREAAYPYFYRMLK
HHHHHHEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEHHHHCCHHHHHHHH
AGARVYRFYQGFYHAKTIVIDEEWCDVGTANFDRRSLFLNGEINCYVFDRAFTRLVKQAI
HHHHHHHHHHHHHHEEEEEEEHHHCCCCCCCCCCCEEEECCCEEEEEEHHHHHHHHHHHH
ERDLTRSEPLTLDFWRNRSLLDRGKESISQLISAWL
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]