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The map label for this gene is purR [H]

Identifier: 138896885

GI number: 138896885

Start: 3364904

End: 3365923

Strand: Direct

Name: purR [H]

Synonym: GTNG_3252

Alternate gene names: 138896885

Gene position: 3364904-3365923 (Clockwise)

Preceding gene: 138896878

Following gene: 138896897

Centisome position: 94.78

GC content: 40.2

Gene sequence:

>1020_bases
ATGAAAAAGAAAGTAACGATGCAAGACATTGCAGATCGATTAAATATATCGAAAAATTCCGTATCGCAAGCATTAAGAGG
AAAAGAGGGAGTCAGCGAAGAAACGCGGCGGCTCGTCAAGCGCGTTGCCGAAGAAATGGGGTACCAATATCCAGGCGAGC
GCAAGAAAAAGAAACAAGGAAAAACGGGAAACATCGGATTAATCGCTTCGGATTTGACCTTTTCCTTTAAAAATTTTTTT
GGAGAAATTTATTTGAGCATTGAAAAAGAAGTGATCAAACGGGGCATGAACCTGCACATTCAATCGGTAAATCAGGAACA
AAAAGAACAGCTTATTTTGCCTTCCTTTATCGAAAATAAAATGGTGGACGGAATCTTAATTCTTTCGCACATCAGTACGG
AATACATTAATAAAGTGATCGCAACCGGCATCCCAACCGTACTCGTCGACCACCATCACCCTAATATTCAAGCAGATGCC
GTATTAACCAATAATCGCTTTGGCGCCTATCTTGCCGTTCAGCATTTAATCGAACTAAATCATCGCGACATCGCTTTTGT
TGGCAATATCAATTATTCACCAAGCTATGAAGAACGATATGAAGGGTACTTGCTCGCCTTGAGAGACTATGGAATTAAAC
CGAACGAAGATTTTATTTTTAAAAATGCGGAAGAAAAAGAAGAAGTCATATTCCGATATATTCAACAATTAAAAAAACAG
CCAACCGCATGGTTTTGCGCCAATGACGCATTAGGATTTCTCGTTAATTCATGCCTGCAAAAGCAAGGCATTCAAGTTCC
AGATCAAGCTTCTGTGTGCAGCTATGATAATGGACAGCTGTCAAAAATAGCGACACCAAAAACAACAACCGTTGACATTG
ATTTGGAGTTATATGGAAGAAGAGCGGTAGAGCTACTGTTCTGGAGAATGGAGAACAAAAACGAACCGGTTCAAGAAATC
CTCCTCTCTTCCAAACTGATTAAAAGGGAATCTACTGCATTAGCGCCAAAACGGTCATGA

Upstream 100 bases:

>100_bases
TGGCAATCATGCCGCCTTAATATGACCTGTTTTTGTTTGTTTAATATTATTGTAATATTTTATATATTTTGTATACGTTA
TAAGATAGTGGGTGTTGAAT

Downstream 100 bases:

>100_bases
AAGTTCTCAGAAAAAAGCTACATTTAAGGGACTGACCCAACACTTTTGGGATTCAGTCTCTTCTTCATTTCATTGGCAAA
AAAAAAAAAAAAATCAACCT

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKKKVTMQDIADRLNISKNSVSQALRGKEGVSEETRRLVKRVAEEMGYQYPGERKKKKQGKTGNIGLIASDLTFSFKNFF
GEIYLSIEKEVIKRGMNLHIQSVNQEQKEQLILPSFIENKMVDGILILSHISTEYINKVIATGIPTVLVDHHHPNIQADA
VLTNNRFGAYLAVQHLIELNHRDIAFVGNINYSPSYEERYEGYLLALRDYGIKPNEDFIFKNAEEKEEVIFRYIQQLKKQ
PTAWFCANDALGFLVNSCLQKQGIQVPDQASVCSYDNGQLSKIATPKTTTVDIDLELYGRRAVELLFWRMENKNEPVQEI
LLSSKLIKRESTALAPKRS

Sequences:

>Translated_339_residues
MKKKVTMQDIADRLNISKNSVSQALRGKEGVSEETRRLVKRVAEEMGYQYPGERKKKKQGKTGNIGLIASDLTFSFKNFF
GEIYLSIEKEVIKRGMNLHIQSVNQEQKEQLILPSFIENKMVDGILILSHISTEYINKVIATGIPTVLVDHHHPNIQADA
VLTNNRFGAYLAVQHLIELNHRDIAFVGNINYSPSYEERYEGYLLALRDYGIKPNEDFIFKNAEEKEEVIFRYIQQLKKQ
PTAWFCANDALGFLVNSCLQKQGIQVPDQASVCSYDNGQLSKIATPKTTTVDIDLELYGRRAVELLFWRMENKNEPVQEI
LLSSKLIKRESTALAPKRS
>Mature_339_residues
MKKKVTMQDIADRLNISKNSVSQALRGKEGVSEETRRLVKRVAEEMGYQYPGERKKKKQGKTGNIGLIASDLTFSFKNFF
GEIYLSIEKEVIKRGMNLHIQSVNQEQKEQLILPSFIENKMVDGILILSHISTEYINKVIATGIPTVLVDHHHPNIQADA
VLTNNRFGAYLAVQHLIELNHRDIAFVGNINYSPSYEERYEGYLLALRDYGIKPNEDFIFKNAEEKEEVIFRYIQQLKKQ
PTAWFCANDALGFLVNSCLQKQGIQVPDQASVCSYDNGQLSKIATPKTTTVDIDLELYGRRAVELLFWRMENKNEPVQEI
LLSSKLIKRESTALAPKRS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=343, Percent_Identity=30.3206997084548, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790369, Length=336, Percent_Identity=28.2738095238095, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790194, Length=343, Percent_Identity=28.2798833819242, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=24.3975903614458, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789068, Length=344, Percent_Identity=26.1627906976744, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=26.3636363636364, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787906, Length=296, Percent_Identity=25, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=25.2336448598131, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=21.3855421686747, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790689, Length=337, Percent_Identity=23.4421364985163, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38714; Mature: 38714

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVTMQDIADRLNISKNSVSQALRGKEGVSEETRRLVKRVAEEMGYQYPGERKKKKQG
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCC
KTGNIGLIASDLTFSFKNFFGEIYLSIEKEVIKRGMNLHIQSVNQEQKEQLILPSFIENK
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHHHHH
MVDGILILSHISTEYINKVIATGIPTVLVDHHHPNIQADAVLTNNRFGAYLAVQHLIELN
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHCC
HRDIAFVGNINYSPSYEERYEGYLLALRDYGIKPNEDFIFKNAEEKEEVIFRYIQQLKKQ
CCCEEEEECCCCCCCHHHHHCCEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHCC
PTAWFCANDALGFLVNSCLQKQGIQVPDQASVCSYDNGQLSKIATPKTTTVDIDLELYGR
CCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEHHHHHH
RAVELLFWRMENKNEPVQEILLSSKLIKRESTALAPKRS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKKVTMQDIADRLNISKNSVSQALRGKEGVSEETRRLVKRVAEEMGYQYPGERKKKKQG
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCC
KTGNIGLIASDLTFSFKNFFGEIYLSIEKEVIKRGMNLHIQSVNQEQKEQLILPSFIENK
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHHHHH
MVDGILILSHISTEYINKVIATGIPTVLVDHHHPNIQADAVLTNNRFGAYLAVQHLIELN
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHCC
HRDIAFVGNINYSPSYEERYEGYLLALRDYGIKPNEDFIFKNAEEKEEVIFRYIQQLKKQ
CCCEEEEECCCCCCCHHHHHCCEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHCC
PTAWFCANDALGFLVNSCLQKQGIQVPDQASVCSYDNGQLSKIATPKTTTVDIDLELYGR
CCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEHHHHHH
RAVELLFWRMENKNEPVQEILLSSKLIKRESTALAPKRS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA