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The map label for this gene is nbaH [H]

Identifier: 138896790

GI number: 138896790

Start: 3254798

End: 3255583

Strand: Reverse

Name: nbaH [H]

Synonym: GTNG_3153

Alternate gene names: 138896790

Gene position: 3255583-3254798 (Counterclockwise)

Preceding gene: 138896791

Following gene: 162330293

Centisome position: 91.7

GC content: 56.23

Gene sequence:

>786_bases
TTGGATGAGATAAATATTGAAAGACTGGCCGATCATTTGGAAGAGGCGGAGAAGAGCGGATGTGGCGTGGCACCGATCAC
TACTTTGGTGCCAGAGATCACCGTAGAGATGGCATATCGTGTGCAGTTAGCCACCATCGAACGCAAGGTGGAGGCTGGCG
ACGTGGTCGTCGGCAAGAAGATCGGTTTGACGTCACTTGCCATGCAGCAGCTGCTCAGTGTCGATCAGCCGGACTATGGT
CACTTGTTAGCCAGCATGGCGGTGGAAAACGGCGGTGAGATCAGTTGGAAACGGGTGATTCAGCCCAAGGTGGAGGCGGA
GATCGCGTTCGTGTTGAAACAGGAGCTGGTCGGTCCCCACGTCACCGCTCTCGATGTGCTACGGGCGACAGATTATGTAA
TTCCTGCATTGGAGATCGTGGACAGTCGCATAGAGAGCTGGGAGATCAAGCTGGAGGACACGATCGCCGATAATGCCTCT
TCCGGCTTGTACGTGCTGGGTGGAAAGCCCCTCAAGCCGGATCGGGTCGACCTGGCCCAAATGGGGATGGCGTTGTACAA
GAACGGCGAACTGGTTAACACGGGGGTGGGAGCGGCGGTGCTGGGCCATCCAGCTAACTGCGTGGCCTGGCTTGCGAACA
AGCTGTATGAATTCGGGATTACGCTGAAAGCCGGCGAAGTGATTCTCTCCGGTGCTTTGTCGGCTGCGGTCGATGCCGCA
CCTGGTGACTATTTTCATGCACGGTTCGCCTATCTTGGAGAGGTAGGGGTGAGATTTCGTGGCTAA

Upstream 100 bases:

>100_bases
TATACGGAGCTCAAAACCGTCACGATGCGGTTGTAAACAACCGCCTAGTCAGTCGTCTTTCAAGCTGGGCTTATCTGTAT
ACGGAAGGGAGGAGCCGTGG

Downstream 100 bases:

>100_bases
AGTGAAAGTGGCAATTCTCGGTTCCGGCAATATCGGCACCGATTTGATGATCAAGCTGGAGAGGTCGTCGCATCTGGAGT
TGACTGCGATGATTGGCATC

Product: 2-hydroxypenta-2,4-dienoate hydratase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MDEINIERLADHLEEAEKSGCGVAPITTLVPEITVEMAYRVQLATIERKVEAGDVVVGKKIGLTSLAMQQLLSVDQPDYG
HLLASMAVENGGEISWKRVIQPKVEAEIAFVLKQELVGPHVTALDVLRATDYVIPALEIVDSRIESWEIKLEDTIADNAS
SGLYVLGGKPLKPDRVDLAQMGMALYKNGELVNTGVGAAVLGHPANCVAWLANKLYEFGITLKAGEVILSGALSAAVDAA
PGDYFHARFAYLGEVGVRFRG

Sequences:

>Translated_261_residues
MDEINIERLADHLEEAEKSGCGVAPITTLVPEITVEMAYRVQLATIERKVEAGDVVVGKKIGLTSLAMQQLLSVDQPDYG
HLLASMAVENGGEISWKRVIQPKVEAEIAFVLKQELVGPHVTALDVLRATDYVIPALEIVDSRIESWEIKLEDTIADNAS
SGLYVLGGKPLKPDRVDLAQMGMALYKNGELVNTGVGAAVLGHPANCVAWLANKLYEFGITLKAGEVILSGALSAAVDAA
PGDYFHARFAYLGEVGVRFRG
>Mature_261_residues
MDEINIERLADHLEEAEKSGCGVAPITTLVPEITVEMAYRVQLATIERKVEAGDVVVGKKIGLTSLAMQQLLSVDQPDYG
HLLASMAVENGGEISWKRVIQPKVEAEIAFVLKQELVGPHVTALDVLRATDYVIPALEIVDSRIESWEIKLEDTIADNAS
SGLYVLGGKPLKPDRVDLAQMGMALYKNGELVNTGVGAAVLGHPANCVAWLANKLYEFGITLKAGEVILSGALSAAVDAA
PGDYFHARFAYLGEVGVRFRG

Specific function: Converts the product of 2-hydroxy-6-oxo-2,4- heptadienoate hydrolase [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=259, Percent_Identity=41.3127413127413, Blast_Score=206, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 28054; Mature: 28054

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEINIERLADHLEEAEKSGCGVAPITTLVPEITVEMAYRVQLATIERKVEAGDVVVGKK
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
IGLTSLAMQQLLSVDQPDYGHLLASMAVENGGEISWKRVIQPKVEAEIAFVLKQELVGPH
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEHHHHCCCHHHHHHHHHHHHHHCCCC
VTALDVLRATDYVIPALEIVDSRIESWEIKLEDTIADNASSGLYVLGGKPLKPDRVDLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHCCCCCCCEEEECCCCCCCCCHHHHH
MGMALYKNGELVNTGVGAAVLGHPANCVAWLANKLYEFGITLKAGEVILSGALSAAVDAA
HHHHHHCCCCEEECCCCHHHHCCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHCCC
PGDYFHARFAYLGEVGVRFRG
CCCHHHHHHHHHHCCCCEECC
>Mature Secondary Structure
MDEINIERLADHLEEAEKSGCGVAPITTLVPEITVEMAYRVQLATIERKVEAGDVVVGKK
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
IGLTSLAMQQLLSVDQPDYGHLLASMAVENGGEISWKRVIQPKVEAEIAFVLKQELVGPH
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEHHHHCCCHHHHHHHHHHHHHHCCCC
VTALDVLRATDYVIPALEIVDSRIESWEIKLEDTIADNASSGLYVLGGKPLKPDRVDLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHCCCCCCCEEEECCCCCCCCCHHHHH
MGMALYKNGELVNTGVGAAVLGHPANCVAWLANKLYEFGITLKAGEVILSGALSAAVDAA
HHHHHHCCCCEEECCCCHHHHCCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHCCC
PGDYFHARFAYLGEVGVRFRG
CCCHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670929 [H]