Click here to switch to the map view.

The map label for this gene is pdhC [H]

Identifier: 138896776

GI number: 138896776

Start: 3241166

End: 3242491

Strand: Reverse

Name: pdhC [H]

Synonym: GTNG_3139

Alternate gene names: 138896776

Gene position: 3242491-3241166 (Counterclockwise)

Preceding gene: 138896782

Following gene: 138896775

Centisome position: 91.33

GC content: 56.18

Gene sequence:

>1326_bases
ATGGCCGTGATTTATGAGTTTAAGCTTCCTGATATTGGCGAAGGGCTGCATGAAGCCGAGATCATCCGTTGGCTCGTCCG
CGAAGGGGATGTGGTCAATGCCGATCAACCAATTGCTGAAATTCAAACGGATAAGGCTATGGTCGAAATGACGACGCCGG
TGGCTGGAAAAGTGATGTCACTTGCCGGGCCGGAAGGAGCCACGGTTAACGTCGGGGAGCCGCTGATTGTATTAGATACC
GAAGCAGCGGGAGAACCGAGAGGAAATCAATCAGAACAAAGCACTGGTCTTAAGGAAACATCGGCAACCGTCCAAGCGGA
TCGGGGAACGCGTCCAGCCCGCAAGCGGGTGATCGCAGCGCCATCGGTGCGCAAGCGGGCGCGGGAGATGGGCGTCCCGA
TTGAGGAAGTGGAAGGAACAGGCGAAGGCGGTCGGGTGACGCTCGCCGATTTGGAGCGATATGTGCGAGAGCGCGAGGCA
GCCGTGACGGTGGCCGAAACGGTACAGAGCGGAATCGGCAAGGTCGAAGAAGCGTCTTTCGCTCGCAGCTCTCATGCCGT
CAGCGGCCGTATAAGCAAGGCGCTGTTTGCGCCACCTTCGACTGGGCCGTCTCCTCTTACTGAGGAAGAAGAACGGATCC
CGCTTCGCGGCTTGCGTAAAAAAATCGCCGAAAAAATGGTGAAATCGGTGTATACGGCGCCGCATGTGACCGGGATGGAT
GAAATCGATGTGACAAAGCTTGTTGAGATCCGCAAGAGCTTAGCCGCCCAATTGGCTGAAGAACGGATCAAACTCACCTA
TTTGCCGTTTGTCATTAAGGCGGTGACAAGGGCATTGAAGCAATACCCGATGTTTAACGCCACGCTTGATGAAGAGACGA
ACGAAATCGTGCTGAAGAAACGTTACCACATCGGCATCGCAACGGCGACAAAAGCCGGACTGTTGGTTCCTGTCATCCGC
GACGCCGATCAAAAATCAATTCGCGAGCTGGCGATTGAGATCGCCGAGCTTTTGGAGAAAGCGCACCGCCAGGCGCTTCG
CCTTGAGGAACTGCAAGGAAGCACGTTCACGATCACGAGCACCGGCGCCGGTGGGGGATGGTTTGCGACGCCGGTCATCA
ACTATCCGGAAGTCGCGATTTTCGGCGCGCATGCGATCAAGCGGCGACCGGTTGTGATCGATGATGAGATTGTCATCCGC
GACATAATGGGGATGTCGCTCACGTTTGATCATCGCGTCATTGACGGCGAGCCGGCCGGGCGGTTTATGCGGACGGTGGC
GCATTATTTGGAGAATCCGGAACTGTTGCTGCTGGATGTGCGGTAG

Upstream 100 bases:

>100_bases
TAAGCGCTTAACCTCTGTGATGCCTCATGATCGCAACACCATCGATGCTGCACCATCGCGTTTGTTTCCAATGCCAATTT
GAGAATAAGAGGAAGGGGAT

Downstream 100 bases:

>100_bases
GCGGCCGTTAGTTCACTTTCGTTTTGTGAACCGAATAAGACTGTGAGTCGCAAAAAATGATGGGAGGGGATCGCATGTTT
GATCCAAAGGAGCTGCCCGT

Product: dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAVIYEFKLPDIGEGLHEAEIIRWLVREGDVVNADQPIAEIQTDKAMVEMTTPVAGKVMSLAGPEGATVNVGEPLIVLDT
EAAGEPRGNQSEQSTGLKETSATVQADRGTRPARKRVIAAPSVRKRAREMGVPIEEVEGTGEGGRVTLADLERYVREREA
AVTVAETVQSGIGKVEEASFARSSHAVSGRISKALFAPPSTGPSPLTEEEERIPLRGLRKKIAEKMVKSVYTAPHVTGMD
EIDVTKLVEIRKSLAAQLAEERIKLTYLPFVIKAVTRALKQYPMFNATLDEETNEIVLKKRYHIGIATATKAGLLVPVIR
DADQKSIRELAIEIAELLEKAHRQALRLEELQGSTFTITSTGAGGGWFATPVINYPEVAIFGAHAIKRRPVVIDDEIVIR
DIMGMSLTFDHRVIDGEPAGRFMRTVAHYLENPELLLLDVR

Sequences:

>Translated_441_residues
MAVIYEFKLPDIGEGLHEAEIIRWLVREGDVVNADQPIAEIQTDKAMVEMTTPVAGKVMSLAGPEGATVNVGEPLIVLDT
EAAGEPRGNQSEQSTGLKETSATVQADRGTRPARKRVIAAPSVRKRAREMGVPIEEVEGTGEGGRVTLADLERYVREREA
AVTVAETVQSGIGKVEEASFARSSHAVSGRISKALFAPPSTGPSPLTEEEERIPLRGLRKKIAEKMVKSVYTAPHVTGMD
EIDVTKLVEIRKSLAAQLAEERIKLTYLPFVIKAVTRALKQYPMFNATLDEETNEIVLKKRYHIGIATATKAGLLVPVIR
DADQKSIRELAIEIAELLEKAHRQALRLEELQGSTFTITSTGAGGGWFATPVINYPEVAIFGAHAIKRRPVVIDDEIVIR
DIMGMSLTFDHRVIDGEPAGRFMRTVAHYLENPELLLLDVR
>Mature_440_residues
AVIYEFKLPDIGEGLHEAEIIRWLVREGDVVNADQPIAEIQTDKAMVEMTTPVAGKVMSLAGPEGATVNVGEPLIVLDTE
AAGEPRGNQSEQSTGLKETSATVQADRGTRPARKRVIAAPSVRKRAREMGVPIEEVEGTGEGGRVTLADLERYVREREAA
VTVAETVQSGIGKVEEASFARSSHAVSGRISKALFAPPSTGPSPLTEEEERIPLRGLRKKIAEKMVKSVYTAPHVTGMDE
IDVTKLVEIRKSLAAQLAEERIKLTYLPFVIKAVTRALKQYPMFNATLDEETNEIVLKKRYHIGIATATKAGLLVPVIRD
ADQKSIRELAIEIAELLEKAHRQALRLEELQGSTFTITSTGAGGGWFATPVINYPEVAIFGAHAIKRRPVVIDDEIVIRD
IMGMSLTFDHRVIDGEPAGRFMRTVAHYLENPELLLLDVR

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=441, Percent_Identity=33.3333333333333, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI19923748, Length=246, Percent_Identity=36.5853658536585, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI31711992, Length=458, Percent_Identity=25.9825327510917, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=26.9911504424779, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=26.9911504424779, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.0248447204969, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1786946, Length=442, Percent_Identity=29.4117647058824, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786305, Length=447, Percent_Identity=31.3199105145414, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=447, Percent_Identity=32.662192393736, Blast_Score=224, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI25146366, Length=432, Percent_Identity=30.3240740740741, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=25.2232142857143, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=230, Percent_Identity=27.8260869565217, Blast_Score=98, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=431, Percent_Identity=31.322505800464, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6324258, Length=463, Percent_Identity=26.133909287257, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI18859875, Length=456, Percent_Identity=31.5789473684211, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24645909, Length=243, Percent_Identity=35.3909465020576, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=26.6094420600858, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=26.6094420600858, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48292; Mature: 48161

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIYEFKLPDIGEGLHEAEIIRWLVREGDVVNADQPIAEIQTDKAMVEMTTPVAGKVMS
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEECCCCHHHHHC
LAGPEGATVNVGEPLIVLDTEAAGEPRGNQSEQSTGLKETSATVQADRGTRPARKRVIAA
CCCCCCCEEECCCCEEEEECCCCCCCCCCCCHHHCCCHHHCCEEECCCCCCHHHHHEECC
PSVRKRAREMGVPIEEVEGTGEGGRVTLADLERYVREREAAVTVAETVQSGIGKVEEASF
CHHHHHHHHHCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ARSSHAVSGRISKALFAPPSTGPSPLTEEEERIPLRGLRKKIAEKMVKSVYTAPHVTGMD
HHHHHHHHHHHHHHEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
EIDVTKLVEIRKSLAAQLAEERIKLTYLPFVIKAVTRALKQYPMFNATLDEETNEIVLKK
HHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
RYHIGIATATKAGLLVPVIRDADQKSIRELAIEIAELLEKAHRQALRLEELQGSTFTITS
EEEEEEEECCCCCEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
TGAGGGWFATPVINYPEVAIFGAHAIKRRPVVIDDEIVIRDIMGMSLTFDHRVIDGEPAG
CCCCCCEEECCCCCCCCEEEECHHHHCCCCEEECCHHHHHHHHCCEEEECCEEECCCCHH
RFMRTVAHYLENPELLLLDVR
HHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
AVIYEFKLPDIGEGLHEAEIIRWLVREGDVVNADQPIAEIQTDKAMVEMTTPVAGKVMS
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEECCCCHHHHHC
LAGPEGATVNVGEPLIVLDTEAAGEPRGNQSEQSTGLKETSATVQADRGTRPARKRVIAA
CCCCCCCEEECCCCEEEEECCCCCCCCCCCCHHHCCCHHHCCEEECCCCCCHHHHHEECC
PSVRKRAREMGVPIEEVEGTGEGGRVTLADLERYVREREAAVTVAETVQSGIGKVEEASF
CHHHHHHHHHCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ARSSHAVSGRISKALFAPPSTGPSPLTEEEERIPLRGLRKKIAEKMVKSVYTAPHVTGMD
HHHHHHHHHHHHHHEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
EIDVTKLVEIRKSLAAQLAEERIKLTYLPFVIKAVTRALKQYPMFNATLDEETNEIVLKK
HHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
RYHIGIATATKAGLLVPVIRDADQKSIRELAIEIAELLEKAHRQALRLEELQGSTFTITS
EEEEEEEECCCCCEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
TGAGGGWFATPVINYPEVAIFGAHAIKRRPVVIDDEIVIRDIMGMSLTFDHRVIDGEPAG
CCCCCCEEECCCCCCCCEEEECHHHHCCCCEEECCHHHHHHHHCCEEEECCEEECCCCHH
RFMRTVAHYLENPELLLLDVR
HHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]