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The map label for this gene is amiA [C]

Identifier: 138896739

GI number: 138896739

Start: 3203411

End: 3204673

Strand: Reverse

Name: amiA [C]

Synonym: GTNG_3102

Alternate gene names: 138896739

Gene position: 3204673-3203411 (Counterclockwise)

Preceding gene: 138896740

Following gene: 138896738

Centisome position: 90.26

GC content: 47.27

Gene sequence:

>1263_bases
GTGCTAGGGAGTGTATCGGGCTATTTTTATCCGTCGAAACATGTAACAAGGGCGGAAGCTGCCGCCATTATTGGGCGGAC
GTTAAACTTTGACGGGAAGCAGCGCGACACCCGTTTCAAAGATGTTGGAAAAGGAAATTTTGCCTCTGGATACATACAAG
AAGCAGCTGAGCGCAACATTATCAGCGGCTACAAAGATGGGACGTTCCGACCTAATCAACTTGTAACGCGTGGGGAAATG
GCCCTGCTCATTAATCGGGCTTATTCGCTTGGCGGAACTACACGATCAACGGCGGTGCAGCAATTGAAAATCAAGGGAAT
CGCGGAAGGGAAAGCTGATGGCACATTTGGTGAAGATGAGCCGATTATTCGGGCGGACTTTGCTGTATTTGTTGCAAGAG
CCATCAATCCGCAATATCGTGTGAATGGTGGCGAGCTGACACCGATCGGTCAAGCCGTTGTCAACGCTTCGTCATTGAAT
GTCCGACGCGGTCCGTCTACGAGTTATAGCGCCGTAAGTCTATTATATAAAGGACAGTCTGTCGATATTTTACATATTGT
TGGCAACTGGGCGTATATTCGTGCATCAGACTTAGAAGGTTTCGTAAGTCGCAGTTATTTATCGGCCAAGGGTGCTTCAC
AGCCTGACGCAGGAGATGCGCTATCAAACTATGTAAAAACGCAAACGATCATTATTGACCCTGGGCATGGAGGCAATGAC
CCTGGTGCGACAGCAAATGGTCTTGTGGAAAAAAATATTAATTTGAATGTCGCACTGAAAGTGAAATCGTTGTTTGAAGG
TACGGGCTTTAACATCGCCCTAACGAGGGAGAAAGATGTATTCATTCCGCTCAGCGAGCGCGTCTCCTTTGCGAAAGAGA
AGGGAGGAAATGTGTTTGTCAGCATTCATACGAATGCTGGGGGTGGAACGGGAACAGAGACGTATTATTATAGTGCTGCA
GCAACAAATCCATATGTCGAACAAAGCAAAAAGCTGGCGCAGTGTATTCAAAAGCGGCTCGTCGCGGCATGGGATGCTGT
TGACCGTGGGACAAAAAGAGGCAATTTGCACGTATTGCGCGAAAACAGTATGCCCGCTGCATTAGTGGAGCTAGGTTTTA
TTGATCGGAAGGAAGATGCACAGAAGCTTGGATCGTCGTATTGGCAAGAACAAGCGGCTAAAGCTATTTATCTTGGTATC
CTTGACTACTATGCCTCAGAAACCGGTTTGAACTTTCAACCGTTATATGATCGGGTTCAGTAG

Upstream 100 bases:

>100_bases
GATGCCGATGAGCCATCCGCAGGCTGCACCGTATTTTCACGACGTGGGCACGACCCACAGGGCAAAAAATGAAATTTATT
ACTTAGCCGAAGGGGGAATT

Downstream 100 bases:

>100_bases
CTGCGAAGACAATGCGCCTTCCGATTCATCGAAGTTTTTTGACTATGGAATTGGCTGCTGAAACATAGCGGCTTGACCCA
GCTAGTGCGTGAGTTGGGGA

Product: surface-layer N-acetylmuramoyl-L-alanine amidase, pXO2-42

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MLGSVSGYFYPSKHVTRAEAAAIIGRTLNFDGKQRDTRFKDVGKGNFASGYIQEAAERNIISGYKDGTFRPNQLVTRGEM
ALLINRAYSLGGTTRSTAVQQLKIKGIAEGKADGTFGEDEPIIRADFAVFVARAINPQYRVNGGELTPIGQAVVNASSLN
VRRGPSTSYSAVSLLYKGQSVDILHIVGNWAYIRASDLEGFVSRSYLSAKGASQPDAGDALSNYVKTQTIIIDPGHGGND
PGATANGLVEKNINLNVALKVKSLFEGTGFNIALTREKDVFIPLSERVSFAKEKGGNVFVSIHTNAGGGTGTETYYYSAA
ATNPYVEQSKKLAQCIQKRLVAAWDAVDRGTKRGNLHVLRENSMPAALVELGFIDRKEDAQKLGSSYWQEQAAKAIYLGI
LDYYASETGLNFQPLYDRVQ

Sequences:

>Translated_420_residues
MLGSVSGYFYPSKHVTRAEAAAIIGRTLNFDGKQRDTRFKDVGKGNFASGYIQEAAERNIISGYKDGTFRPNQLVTRGEM
ALLINRAYSLGGTTRSTAVQQLKIKGIAEGKADGTFGEDEPIIRADFAVFVARAINPQYRVNGGELTPIGQAVVNASSLN
VRRGPSTSYSAVSLLYKGQSVDILHIVGNWAYIRASDLEGFVSRSYLSAKGASQPDAGDALSNYVKTQTIIIDPGHGGND
PGATANGLVEKNINLNVALKVKSLFEGTGFNIALTREKDVFIPLSERVSFAKEKGGNVFVSIHTNAGGGTGTETYYYSAA
ATNPYVEQSKKLAQCIQKRLVAAWDAVDRGTKRGNLHVLRENSMPAALVELGFIDRKEDAQKLGSSYWQEQAAKAIYLGI
LDYYASETGLNFQPLYDRVQ
>Mature_420_residues
MLGSVSGYFYPSKHVTRAEAAAIIGRTLNFDGKQRDTRFKDVGKGNFASGYIQEAAERNIISGYKDGTFRPNQLVTRGEM
ALLINRAYSLGGTTRSTAVQQLKIKGIAEGKADGTFGEDEPIIRADFAVFVARAINPQYRVNGGELTPIGQAVVNASSLN
VRRGPSTSYSAVSLLYKGQSVDILHIVGNWAYIRASDLEGFVSRSYLSAKGASQPDAGDALSNYVKTQTIIIDPGHGGND
PGATANGLVEKNINLNVALKVKSLFEGTGFNIALTREKDVFIPLSERVSFAKEKGGNVFVSIHTNAGGGTGTETYYYSAA
ATNPYVEQSKKLAQCIQKRLVAAWDAVDRGTKRGNLHVLRENSMPAALVELGFIDRKEDAQKLGSSYWQEQAAKAIYLGI
LDYYASETGLNFQPLYDRVQ

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall, S-layer (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=240, Percent_Identity=27.9166666666667, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082163, Length=224, Percent_Identity=29.0178571428571, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790611, Length=246, Percent_Identity=27.6422764227642, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR001119 [H]

Pfam domain/function: PF01520 Amidase_3; PF00395 SLH [H]

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 45494; Mature: 45494

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGSVSGYFYPSKHVTRAEAAAIIGRTLNFDGKQRDTRFKDVGKGNFASGYIQEAAERNI
CCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCC
ISGYKDGTFRPNQLVTRGEMALLINRAYSLGGTTRSTAVQQLKIKGIAEGKADGTFGEDE
CCCCCCCCCCCCHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHEEEEEECCCCCCCCCCCC
PIIRADFAVFVARAINPQYRVNGGELTPIGQAVVNASSLNVRRGPSTSYSAVSLLYKGQS
CEEHHHHHHHHHHHCCCCEEECCCCCCCHHHHHCCCCCCEEECCCCCCHHEEEEEECCCC
VDILHIVGNWAYIRASDLEGFVSRSYLSAKGASQPDAGDALSNYVKTQTIIIDPGHGGND
EEEEEEECCEEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEEEEECCCCCCC
PGATANGLVEKNINLNVALKVKSLFEGTGFNIALTREKDVFIPLSERVSFAKEKGGNVFV
CCCCCCCEEECCCCEEEEEEEEHHHCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEE
SIHTNAGGGTGTETYYYSAAATNPYVEQSKKLAQCIQKRLVAAWDAVDRGTKRGNLHVLR
EEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
ENSMPAALVELGFIDRKEDAQKLGSSYWQEQAAKAIYLGILDYYASETGLNFQPLYDRVQ
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MLGSVSGYFYPSKHVTRAEAAAIIGRTLNFDGKQRDTRFKDVGKGNFASGYIQEAAERNI
CCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCC
ISGYKDGTFRPNQLVTRGEMALLINRAYSLGGTTRSTAVQQLKIKGIAEGKADGTFGEDE
CCCCCCCCCCCCHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHEEEEEECCCCCCCCCCCC
PIIRADFAVFVARAINPQYRVNGGELTPIGQAVVNASSLNVRRGPSTSYSAVSLLYKGQS
CEEHHHHHHHHHHHCCCCEEECCCCCCCHHHHHCCCCCCEEECCCCCCHHEEEEEECCCC
VDILHIVGNWAYIRASDLEGFVSRSYLSAKGASQPDAGDALSNYVKTQTIIIDPGHGGND
EEEEEEECCEEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEEEEECCCCCCC
PGATANGLVEKNINLNVALKVKSLFEGTGFNIALTREKDVFIPLSERVSFAKEKGGNVFV
CCCCCCCEEECCCCEEEEEEEEHHHCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEE
SIHTNAGGGTGTETYYYSAAATNPYVEQSKKLAQCIQKRLVAAWDAVDRGTKRGNLHVLR
EEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
ENSMPAALVELGFIDRKEDAQKLGSSYWQEQAAKAIYLGILDYYASETGLNFQPLYDRVQ
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10475962; 12004073 [H]