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The map label for this gene is aprN [H]

Identifier: 138896738

GI number: 138896738

Start: 3201378

End: 3203297

Strand: Reverse

Name: aprN [H]

Synonym: GTNG_3101

Alternate gene names: 138896738

Gene position: 3203297-3201378 (Counterclockwise)

Preceding gene: 138896739

Following gene: 138896736

Centisome position: 90.23

GC content: 50.83

Gene sequence:

>1920_bases
ATGAAAAAGTGGTGGATGTTGACCATCTGTATTGTGTTTATAGTTGCAGCAGTAGTACCAGTTCGTGCAGCTGCTGCTTC
TCATCCATTGTTTGCCAATGTCATTGTCGTGTTTCAACAACAAGTGGATGAACAAGCAGTGAAAACGTTGCATGGGAATG
TCACCAAACGGTTTGAAGTGATTCCCGCGGTGGCAGCAACACTTCCTGTTACGGCTGTCGAGTTGTTACGCCGTTGGCCG
GGGGTTGATTATGTACAGCAAGATACAACGGTGGCAATTGATGGCCAGATCACCGACTGGGGAGTGGAAAAGGTGAAAGC
GCCCGTGATGCACACCCACGGTGTAACAGGAAAAGGAGTGAAAATCGCGATTTTAGATACGGGCGTTGACCTAAAGCACC
CGGATTTGCGTGTAGTGGGCGGCGCTTGCGTGTTGTCCTATTGCCCTAATTCCTATCAAGACGATAACGGGCATGGCACT
CACGTAGCCGGGATTATTGCGGCAAAAGATAACGATATCGGGACGGTCGGGGTGGCTCCCAATGCGAGTATTTATGCAGT
GAAGGTGCTAGACCGTTACGGCGAGGGAAATGTATCCGATATTTTATCAGGCATCGAATGGGCAATGAAGCATCGTGTAG
ATATAATTAATTTAAGCTTAGCTGCTCCAGAAGATGCTCCTGCCTTGAAAGCGGCCATTCGGAAAGCGTACGAAAGCGGG
ATTTTAGTCGTTGCTGCCGCTGGTAACAACGGCTATGCTAACGGCACAGGTGATACGGTCGAATATCCAGCGAAGTATGA
TAGTGCCATTGCTGTCTCTGCTGTGAATAAAGAAAATGTTCGGTTGCCATACTCGGCGACGGGACCAGCGATCGAAGTAG
CGGCTCCTGGGGAGGGTGTTTATAGCACTGTGCCGGTTGCTCTTGACGGTGATGGCCGTCGTGACGGATATACGCATATG
TCTGGAACGTCGATGGCGGCGCCATTTGTCACAGGGGTGCTCGCTTTGTATAAGCAACAATATCCGGAGCGGACGAATAT
CGAATTGCGCCAAATGTTAAGAGATCGTGCCTTGGATTTAGGGACACCCGGGAGGGATCCGTGGTACGGCTATGGACTCG
TACGGGCCGTGTCCAACCAACCGCCCGAGCTGAAAGTGAAGCTATTGTCGGTGAAAAAAGGAGAAGTCGCTTTTTCCGTC
ACCTTGTCAGGAAGCGCAGTAAAAGGGTATCGTGTATATCGGAACGGAAAACAGATCGAAACGCTGCAAACAGCGGGGAC
GTATACAGACTATGTCGTTAAAGGAACATACCAGTACGAATTTGCTTCGATACGCAATGATGGGGCAGAATCAGCATTAA
CAGCTCCCATCACGGTGAACGTACCGGATCCCTATTATAAAGACGTGTCAGCGAGCGCTTGGTATATGCCGGAGATTGCC
TACTTATCAAGCCGAGGGATAGTGTCCGGCTATAACAATGGAACGATTCAGCCTTATAATACGATCACGCGTGCCGAAGT
AGCGGTGATGCTTGGTCGGGCGCTGCATCTTGATGCGACAAAGCGAGCGACGGTGTTTCGCGATGTTGCTTCATCCGATT
TCGCCTCTGGTTACATTCAATCCGCCTATGAGCGCGGGCTGATCAACGGGTATCCGGATGGAACGTTTCGACCACAGCAG
CCGATTACGCGTGCTGAGACAGCTATCATTTTATCGCGGGCTTATCCACTGCCGGGCGGTTCATCGATGATGTTTAAAGA
TGTGACGGCAAGAGTGACCGGGCATGAGGCCATCGCCAAGCTCGCTGCCGCCCGAATCGCGGAAGGATATCCCGATGGAA
CGTTTCGGCCATATCAGTTTGTCAAACGACTTGAATTTTTTGTCTTTACGGCTCGTGCCGATAACGAGCGGTTTCGGTGA

Upstream 100 bases:

>100_bases
GCGCCTTCCGATTCATCGAAGTTTTTTGACTATGGAATTGGCTGCTGAAACATAGCGGCTTGACCCAGCTAGTGCGTGAG
TTGGGGAGAGGGGTCAGATA

Downstream 100 bases:

>100_bases
AACGAAGAGGTTGGTTGGGCAGATTGTTTCTTCTCGCAAAATAACCGCTCGTTCCACAACTATGGCTGAATGTGCATAGA
CAGGAAAAAGGTGTCCCAGC

Product: alkaline serine proteinase

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MKKWWMLTICIVFIVAAVVPVRAAAASHPLFANVIVVFQQQVDEQAVKTLHGNVTKRFEVIPAVAATLPVTAVELLRRWP
GVDYVQQDTTVAIDGQITDWGVEKVKAPVMHTHGVTGKGVKIAILDTGVDLKHPDLRVVGGACVLSYCPNSYQDDNGHGT
HVAGIIAAKDNDIGTVGVAPNASIYAVKVLDRYGEGNVSDILSGIEWAMKHRVDIINLSLAAPEDAPALKAAIRKAYESG
ILVVAAAGNNGYANGTGDTVEYPAKYDSAIAVSAVNKENVRLPYSATGPAIEVAAPGEGVYSTVPVALDGDGRRDGYTHM
SGTSMAAPFVTGVLALYKQQYPERTNIELRQMLRDRALDLGTPGRDPWYGYGLVRAVSNQPPELKVKLLSVKKGEVAFSV
TLSGSAVKGYRVYRNGKQIETLQTAGTYTDYVVKGTYQYEFASIRNDGAESALTAPITVNVPDPYYKDVSASAWYMPEIA
YLSSRGIVSGYNNGTIQPYNTITRAEVAVMLGRALHLDATKRATVFRDVASSDFASGYIQSAYERGLINGYPDGTFRPQQ
PITRAETAIILSRAYPLPGGSSMMFKDVTARVTGHEAIAKLAAARIAEGYPDGTFRPYQFVKRLEFFVFTARADNERFR

Sequences:

>Translated_639_residues
MKKWWMLTICIVFIVAAVVPVRAAAASHPLFANVIVVFQQQVDEQAVKTLHGNVTKRFEVIPAVAATLPVTAVELLRRWP
GVDYVQQDTTVAIDGQITDWGVEKVKAPVMHTHGVTGKGVKIAILDTGVDLKHPDLRVVGGACVLSYCPNSYQDDNGHGT
HVAGIIAAKDNDIGTVGVAPNASIYAVKVLDRYGEGNVSDILSGIEWAMKHRVDIINLSLAAPEDAPALKAAIRKAYESG
ILVVAAAGNNGYANGTGDTVEYPAKYDSAIAVSAVNKENVRLPYSATGPAIEVAAPGEGVYSTVPVALDGDGRRDGYTHM
SGTSMAAPFVTGVLALYKQQYPERTNIELRQMLRDRALDLGTPGRDPWYGYGLVRAVSNQPPELKVKLLSVKKGEVAFSV
TLSGSAVKGYRVYRNGKQIETLQTAGTYTDYVVKGTYQYEFASIRNDGAESALTAPITVNVPDPYYKDVSASAWYMPEIA
YLSSRGIVSGYNNGTIQPYNTITRAEVAVMLGRALHLDATKRATVFRDVASSDFASGYIQSAYERGLINGYPDGTFRPQQ
PITRAETAIILSRAYPLPGGSSMMFKDVTARVTGHEAIAKLAAARIAEGYPDGTFRPYQFVKRLEFFVFTARADNERFR
>Mature_639_residues
MKKWWMLTICIVFIVAAVVPVRAAAASHPLFANVIVVFQQQVDEQAVKTLHGNVTKRFEVIPAVAATLPVTAVELLRRWP
GVDYVQQDTTVAIDGQITDWGVEKVKAPVMHTHGVTGKGVKIAILDTGVDLKHPDLRVVGGACVLSYCPNSYQDDNGHGT
HVAGIIAAKDNDIGTVGVAPNASIYAVKVLDRYGEGNVSDILSGIEWAMKHRVDIINLSLAAPEDAPALKAAIRKAYESG
ILVVAAAGNNGYANGTGDTVEYPAKYDSAIAVSAVNKENVRLPYSATGPAIEVAAPGEGVYSTVPVALDGDGRRDGYTHM
SGTSMAAPFVTGVLALYKQQYPERTNIELRQMLRDRALDLGTPGRDPWYGYGLVRAVSNQPPELKVKLLSVKKGEVAFSV
TLSGSAVKGYRVYRNGKQIETLQTAGTYTDYVVKGTYQYEFASIRNDGAESALTAPITVNVPDPYYKDVSASAWYMPEIA
YLSSRGIVSGYNNGTIQPYNTITRAEVAVMLGRALHLDATKRATVFRDVASSDFASGYIQSAYERGLINGYPDGTFRPQQ
PITRAETAIILSRAYPLPGGSSMMFKDVTARVTGHEAIAKLAAARIAEGYPDGTFRPYQFVKRLEFFVFTARADNERFR

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=243, Percent_Identity=28.8065843621399, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI31317307, Length=338, Percent_Identity=28.4023668639053, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI76443679, Length=297, Percent_Identity=26.2626262626263, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI27894285, Length=304, Percent_Identity=28.9473684210526, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI20336180, Length=304, Percent_Identity=28.9473684210526, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI20336190, Length=304, Percent_Identity=28.9473684210526, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI4505577, Length=305, Percent_Identity=29.5081967213115, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI20336188, Length=304, Percent_Identity=28.9473684210526, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI20336184, Length=304, Percent_Identity=28.9473684210526, Blast_Score=66, Evalue=8e-11,
Organism=Homo sapiens, GI20336186, Length=270, Percent_Identity=29.2592592592593, Blast_Score=66, Evalue=9e-11,
Organism=Homo sapiens, GI20336182, Length=304, Percent_Identity=28.9473684210526, Blast_Score=66, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6324576, Length=324, Percent_Identity=31.4814814814815, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6320775, Length=298, Percent_Identity=34.5637583892617, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319893, Length=241, Percent_Identity=32.3651452282158, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550681, Length=297, Percent_Identity=26.2626262626263, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136192, Length=263, Percent_Identity=29.6577946768061, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281360987, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642494, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642490, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45555723, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642492, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642488, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642484, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642486, Length=267, Percent_Identity=28.4644194756554, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 69119; Mature: 69119

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS01072 SLH_DOMAIN ; PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWWMLTICIVFIVAAVVPVRAAAASHPLFANVIVVFQQQVDEQAVKTLHGNVTKRFEV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IPAVAATLPVTAVELLRRWPGVDYVQQDTTVAIDGQITDWGVEKVKAPVMHTHGVTGKGV
HHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCHHHHCCCCEEECCCCCCCE
KIAILDTGVDLKHPDLRVVGGACVLSYCPNSYQDDNGHGTHVAGIIAAKDNDIGTVGVAP
EEEEEECCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCC
NASIYAVKVLDRYGEGNVSDILSGIEWAMKHRVDIINLSLAAPEDAPALKAAIRKAYESG
CCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHCC
ILVVAAAGNNGYANGTGDTVEYPAKYDSAIAVSAVNKENVRLPYSATGPAIEVAAPGEGV
EEEEEECCCCCCCCCCCCCEECCCCCCCEEEEEEECCCCEEECCCCCCCEEEEECCCCCC
YSTVPVALDGDGRRDGYTHMSGTSMAAPFVTGVLALYKQQYPERTNIELRQMLRDRALDL
CEEEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
GTPGRDPWYGYGLVRAVSNQPPELKVKLLSVKKGEVAFSVTLSGSAVKGYRVYRNGKQIE
CCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEEECCCCEECEEEECCCCEEE
TLQTAGTYTDYVVKGTYQYEFASIRNDGAESALTAPITVNVPDPYYKDVSASAWYMPEIA
EEECCCCEEEEEEEEEEEEEEHHHCCCCCCCEEECCEEEECCCCHHCCCCCCEEECCEEE
YLSSRGIVSGYNNGTIQPYNTITRAEVAVMLGRALHLDATKRATVFRDVASSDFASGYIQ
EECCCCCEECCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHCCHHHHHHHH
SAYERGLINGYPDGTFRPQQPITRAETAIILSRAYPLPGGSSMMFKDVTARVTGHEAIAK
HHHHCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCEEEEHHHHEECCHHHHHH
LAAARIAEGYPDGTFRPYQFVKRLEFFVFTARADNERFR
HHHHHHHCCCCCCCCCHHHHHHHHEEEEEEEECCCCCCC
>Mature Secondary Structure
MKKWWMLTICIVFIVAAVVPVRAAAASHPLFANVIVVFQQQVDEQAVKTLHGNVTKRFEV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IPAVAATLPVTAVELLRRWPGVDYVQQDTTVAIDGQITDWGVEKVKAPVMHTHGVTGKGV
HHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCHHHHCCCCEEECCCCCCCE
KIAILDTGVDLKHPDLRVVGGACVLSYCPNSYQDDNGHGTHVAGIIAAKDNDIGTVGVAP
EEEEEECCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCC
NASIYAVKVLDRYGEGNVSDILSGIEWAMKHRVDIINLSLAAPEDAPALKAAIRKAYESG
CCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHCC
ILVVAAAGNNGYANGTGDTVEYPAKYDSAIAVSAVNKENVRLPYSATGPAIEVAAPGEGV
EEEEEECCCCCCCCCCCCCEECCCCCCCEEEEEEECCCCEEECCCCCCCEEEEECCCCCC
YSTVPVALDGDGRRDGYTHMSGTSMAAPFVTGVLALYKQQYPERTNIELRQMLRDRALDL
CEEEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
GTPGRDPWYGYGLVRAVSNQPPELKVKLLSVKKGEVAFSVTLSGSAVKGYRVYRNGKQIE
CCCCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEEECCCCEECEEEECCCCEEE
TLQTAGTYTDYVVKGTYQYEFASIRNDGAESALTAPITVNVPDPYYKDVSASAWYMPEIA
EEECCCCEEEEEEEEEEEEEEHHHCCCCCCCEEECCEEEECCCCHHCCCCCCEEECCEEE
YLSSRGIVSGYNNGTIQPYNTITRAEVAVMLGRALHLDATKRATVFRDVASSDFASGYIQ
EECCCCCEECCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHCCHHHHHHHH
SAYERGLINGYPDGTFRPQQPITRAETAIILSRAYPLPGGSSMMFKDVTARVTGHEAIAK
HHHHCCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCEEEEHHHHEECCHHHHHH
LAAARIAEGYPDGTFRPYQFVKRLEFFVFTARADNERFR
HHHHHHHCCCCCCCCCHHHHHHHHEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]