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The map label for this gene is prfB [H]

Identifier: 138896684

GI number: 138896684

Start: 3144209

End: 3145192

Strand: Reverse

Name: prfB [H]

Synonym: GTNG_3047

Alternate gene names: 138896684

Gene position: 3145192-3144209 (Counterclockwise)

Preceding gene: 162330294

Following gene: 138896683

Centisome position: 88.59

GC content: 53.96

Gene sequence:

>984_bases
ATGGCCGCGCCCGGCTTTTGGGATGACCAGAAAGCGGCGCAGGCGATCATTTCCGAAGCGAATGCGCTCAAGGAATTAGT
CGGCGAGTTTGAATCGCTCGCGGAACGGTTCGACAACTTGGAAGTGACGTATGAGTTGTTGAAAGAGGAGCCGGATGACG
AGCTGCAGGCTGAACTTGTGGAAGAAGCGAAAAAATTGACGAAAGACTTCAGCCAGTTTGAGCTGCAGCTGTTGCTCAAC
GAGCCGTACGACCAAAATAACGCGATTTTGGAGCTTCATCCGGGTGCGGGCGGCACGGAATCGCAAGACTGGGCGTCGAT
GCTGTTGCGCATGTACACGCGCTGGGCGGAGAAAAAAGGATTTAAAGTCGAAACACTGGATTATCTCCCAGGCGAGGAAG
CCGGGGTGAAAAGCGTCACCTTGCTTATCAAGGGACATAATGCATACGGCTACTTAAAGGCGGAAAAAGGGGTACACCGG
CTTGTGCGCATCTCCCCGTTTGACGCCTCAGGCCGCCGCCATACGTCGTTCGTGTCATGCGAAGTCGTGCCGGAGATGGA
CGATAACATTGAGATTGAGATCCGTCCGGAAGAGCTGAAAATCGACACGTACCGCTCAAGCGGTGCGGGCGGGCAGCACG
TCAACACGACCGACTCCGCGGTGCGCATCACCCACTTGCCGACCGGCATTGTCGTTACGTGCCAATCGGAGCGGTCGCAA
ATTAAAAACCGCGAAAAAGCGATGAATATGTTAAAAGCGAAGCTGTATCAAAAGAAAATGGAGGAACAGCAAGCTGAACT
CGCCGAGCTGCGCGGCGAGCAAAAAGAAATCGGCTGGGGCAGCCAAATCCGCTCCTACGTCTTCCATCCGTATTCGCTTG
TCAAAGACCATCGGACGAATGTGGAGGTCGGCAACGTGCAAGCGGTGATGGATGGGGAAATCGATGTGTTCATTGACGCG
TATTTGCGCGCGAAATTGAAGTAA

Upstream 100 bases:

>100_bases
AAGCAAGAGTTAGAAAAAATGGCTAAGCGATTAGCGGAAATCAGGGGGTCTCTTTGACCTCGAAACAAAGCAGACGCGCA
TTCAAGAATTAGAGGAGCAA

Downstream 100 bases:

>100_bases
AGAGCGATCATCTGATCGCTCTTTTTTTCTTTATCATAGTAGACCATTATCGCGTTAACTGACTGTCATTTACTTAAAAA
AACGGGCATGCTATACTCAC

Product: peptide chain release factor 2, RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAAPGFWDDQKAAQAIISEANALKELVGEFESLAERFDNLEVTYELLKEEPDDELQAELVEEAKKLTKDFSQFELQLLLN
EPYDQNNAILELHPGAGGTESQDWASMLLRMYTRWAEKKGFKVETLDYLPGEEAGVKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDASGRRHTSFVSCEVVPEMDDNIEIEIRPEELKIDTYRSSGAGGQHVNTTDSAVRITHLPTGIVVTCQSERSQ
IKNREKAMNMLKAKLYQKKMEEQQAELAELRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNVEVGNVQAVMDGEIDVFIDA
YLRAKLK

Sequences:

>Translated_327_residues
MAAPGFWDDQKAAQAIISEANALKELVGEFESLAERFDNLEVTYELLKEEPDDELQAELVEEAKKLTKDFSQFELQLLLN
EPYDQNNAILELHPGAGGTESQDWASMLLRMYTRWAEKKGFKVETLDYLPGEEAGVKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDASGRRHTSFVSCEVVPEMDDNIEIEIRPEELKIDTYRSSGAGGQHVNTTDSAVRITHLPTGIVVTCQSERSQ
IKNREKAMNMLKAKLYQKKMEEQQAELAELRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNVEVGNVQAVMDGEIDVFIDA
YLRAKLK
>Mature_326_residues
AAPGFWDDQKAAQAIISEANALKELVGEFESLAERFDNLEVTYELLKEEPDDELQAELVEEAKKLTKDFSQFELQLLLNE
PYDQNNAILELHPGAGGTESQDWASMLLRMYTRWAEKKGFKVETLDYLPGEEAGVKSVTLLIKGHNAYGYLKAEKGVHRL
VRISPFDASGRRHTSFVSCEVVPEMDDNIEIEIRPEELKIDTYRSSGAGGQHVNTTDSAVRITHLPTGIVVTCQSERSQI
KNREKAMNMLKAKLYQKKMEEQQAELAELRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNVEVGNVQAVMDGEIDVFIDAY
LRAKLK

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=305, Percent_Identity=39.672131147541, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI34577120, Length=307, Percent_Identity=35.1791530944625, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI166795305, Length=208, Percent_Identity=40.3846153846154, Blast_Score=141, Evalue=7e-34,
Organism=Escherichia coli, GI2367172, Length=323, Percent_Identity=50.7739938080495, Blast_Score=334, Evalue=4e-93,
Organism=Escherichia coli, GI1787462, Length=318, Percent_Identity=39.622641509434, Blast_Score=210, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=313, Percent_Identity=30.6709265175719, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=312, Percent_Identity=34.6153846153846, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19921226, Length=257, Percent_Identity=40.0778210116732, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37039; Mature: 36908

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPGFWDDQKAAQAIISEANALKELVGEFESLAERFDNLEVTYELLKEEPDDELQAELV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHH
EEAKKLTKDFSQFELQLLLNEPYDQNNAILELHPGAGGTESQDWASMLLRMYTRWAEKKG
HHHHHHHHHHHHHEEHHEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETLDYLPGEEAGVKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDASGRRHTSFVSC
CEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECHHCHHHEEEECCCCCCCCCCCEEEEE
EVVPEMDDNIEIEIRPEELKIDTYRSSGAGGQHVNTTDSAVRITHLPTGIVVTCQSERSQ
EECCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHH
IKNREKAMNMLKAKLYQKKMEEQQAELAELRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHHHCCCC
VEVGNVQAVMDGEIDVFIDAYLRAKLK
CEECCEEEEECCCEEEEEEHHHHHCCC
>Mature Secondary Structure 
AAPGFWDDQKAAQAIISEANALKELVGEFESLAERFDNLEVTYELLKEEPDDELQAELV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHH
EEAKKLTKDFSQFELQLLLNEPYDQNNAILELHPGAGGTESQDWASMLLRMYTRWAEKKG
HHHHHHHHHHHHHEEHHEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETLDYLPGEEAGVKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDASGRRHTSFVSC
CEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECHHCHHHEEEECCCCCCCCCCCEEEEE
EVVPEMDDNIEIEIRPEELKIDTYRSSGAGGQHVNTTDSAVRITHLPTGIVVTCQSERSQ
EECCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHH
IKNREKAMNMLKAKLYQKKMEEQQAELAELRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHHHCCCC
VEVGNVQAVMDGEIDVFIDAYLRAKLK
CEECCEEEEECCCEEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]