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The map label for this gene is menD

Identifier: 138896410

GI number: 138896410

Start: 2886258

End: 2888018

Strand: Reverse

Name: menD

Synonym: GTNG_2773

Alternate gene names: 138896410

Gene position: 2888018-2886258 (Counterclockwise)

Preceding gene: 138896411

Following gene: 138896409

Centisome position: 81.35

GC content: 58.32

Gene sequence:

>1761_bases
ATGACTAACGCGTTGTCATGGTATGTGGCCGCTTTTGTTGACGGGCTTGTGCAAGCCGGGGTGACAGAAGCCGTCATCAG
CCCTGGTTCGCGCTCGACGCCGCTCGCGATGGCGATGGCGGCGCATAGCGGTCTCCACTTTTCCATGCACATTGATGAAC
GATCGGCTGCGTTTTTTGCCCTCGGCATGGTGAAAGCGAAACAGCGGCCGGTCGCTTTAGTGTGCACGTCAGGGACCGCG
GCGGCCAACTATTTGCCGGCCATTGTTGAGGCGTACTATTCGCGCGTGCCGCTTGTCGTGCTGACGGCTGACCGACCGCA
TGAACTGCGCGATGTCGGGGCCCCGCAAGCGATCGACCAACTGCATTTATACGGCCGCTATGCGAAATGGTTTGTCGATC
TGGCGTTGCCGGAAGAAACGGATCCTATGCTGAGCTATGCGCGGACGATGGCGGCAAGGGCCGCCGCCATCGCAGCGGGG
GCGCCGGCCGGACCAGTGCATGTCAACGTTCCGCTCCGCGAGCCGCTTGTCCCGACCATCGACGAGGCCGTTTGGGAGAA
GGTGCGTACGGTTGCGGAAACGCCGCAAATCATGAGCGGCCGTGCGACGCTGCCAGCAGAGAACGTGGCGGCGCTCTATG
AGCAGTTGGCGGCGGCGAAACGCGGGCTCATTGTTTGCGGAGCGCTTGATCAGCCCGGCTTCGCTGAAGCGGTCACAGAG
CTGGCACGGACGCTTGACTTTCCGATTCTTGCTGACCCGCTTTCCCAACTGCGCGCTGGCGCGCATGACAAAACGTATGT
CATCGATAGCTATGATGCCATTTTAAAAGACGAGGCGGTCGCTTCGAGGCTTGTGCCGGATGTCGTGCTCCGGTTCGGCG
CCATGCCGGTATCCAAGCCGCTGTTTTTATGGTTGAAGCAACACCGTTCCATCCGCCAAATTGTCGTCGATGACGGCGGC
TGGCGCGATCCGACGTTGGAAGCGGCGTGCTTCGTCCGCAGCGATGAAACGGTTCTTTGCCGCCAGCTGCTTGACATCGC
TAGACCGAAGCAAAAGGAGAGCGCGTGGTCGACGACATGGCGGGAGATGAACGACATTGCCCGCACCGTGTTGCGGCAGC
ATTTGCCGGCAGACGAATGGTTTGAAGGAAAAGTGTTCACTGAGCTCGCTGAGTTGTTGCCGGCCGGAGCCACCCTTTTT
GTCGGCAACAGCATGCCGATTCGCGACGCCGATACGTTTCTCTTTGCCACGGACAAGCCGCTTCGCGTTCTCGCCAACCG
CGGTGCTAACGGCATTGACGGTGTCGTTTCAAGCGCGTTGGGCGCCAGCCTTGCCGCATCGCCCCTTGTGCTTGTCATTG
GCGATTTGTCGTTTTACCATGATTTAAACGGTCTGCTGGCGGCCAAAATGCATGGACTGCAGGCGACGATTGTGTTGATG
AACAATAACGGCGGCGGCATTTTTTCTTTTTTGCCGCAAGCGCGCCATGAAGGGCCGTTTGAAACGTTGTTTGGCACGCC
GACCGATTTGACGTTTGCTCATGCGGTCGAGATGTATGGCGGCCGTTATGCAGTGCCGCACACATGGGGCGAGTTTCGCC
ACCATGTCGCCGAATCGCTCAACACAGGTGGGTTAAGTGTCATCGAAGTGCGCACGTCGCGCACAGAAAATGTCCAAATG
CATCGATTTTTGTGGGAACGTGTTTCCCAGGAAATCGCCAAATTTCTCGAACAAAAAGGAACGGAAGAACCATGGAATTG
A

Upstream 100 bases:

>100_bases
GCCGGCTGCGGCATCGTCGCCCATTCAGATCCAACGAGCGAGTACGAAGAGACGAACGTCAAAATGGCTCCGATGTTATC
GTCCTTAGGAGTGATGAGCA

Downstream 100 bases:

>100_bases
ATGGAATCCGTTACCATGTAGAACAATATGGGGAAGGGGAGCCGTTATTGCTGTTGCACGGGTTTACCGGCAGTGCGGAT
ACGTGGCGGCCACTCCTTCG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTNALSWYVAAFVDGLVQAGVTEAVISPGSRSTPLAMAMAAHSGLHFSMHIDERSAAFFALGMVKAKQRPVALVCTSGTA
AANYLPAIVEAYYSRVPLVVLTADRPHELRDVGAPQAIDQLHLYGRYAKWFVDLALPEETDPMLSYARTMAARAAAIAAG
APAGPVHVNVPLREPLVPTIDEAVWEKVRTVAETPQIMSGRATLPAENVAALYEQLAAAKRGLIVCGALDQPGFAEAVTE
LARTLDFPILADPLSQLRAGAHDKTYVIDSYDAILKDEAVASRLVPDVVLRFGAMPVSKPLFLWLKQHRSIRQIVVDDGG
WRDPTLEAACFVRSDETVLCRQLLDIARPKQKESAWSTTWREMNDIARTVLRQHLPADEWFEGKVFTELAELLPAGATLF
VGNSMPIRDADTFLFATDKPLRVLANRGANGIDGVVSSALGASLAASPLVLVIGDLSFYHDLNGLLAAKMHGLQATIVLM
NNNGGGIFSFLPQARHEGPFETLFGTPTDLTFAHAVEMYGGRYAVPHTWGEFRHHVAESLNTGGLSVIEVRTSRTENVQM
HRFLWERVSQEIAKFLEQKGTEEPWN

Sequences:

>Translated_586_residues
MTNALSWYVAAFVDGLVQAGVTEAVISPGSRSTPLAMAMAAHSGLHFSMHIDERSAAFFALGMVKAKQRPVALVCTSGTA
AANYLPAIVEAYYSRVPLVVLTADRPHELRDVGAPQAIDQLHLYGRYAKWFVDLALPEETDPMLSYARTMAARAAAIAAG
APAGPVHVNVPLREPLVPTIDEAVWEKVRTVAETPQIMSGRATLPAENVAALYEQLAAAKRGLIVCGALDQPGFAEAVTE
LARTLDFPILADPLSQLRAGAHDKTYVIDSYDAILKDEAVASRLVPDVVLRFGAMPVSKPLFLWLKQHRSIRQIVVDDGG
WRDPTLEAACFVRSDETVLCRQLLDIARPKQKESAWSTTWREMNDIARTVLRQHLPADEWFEGKVFTELAELLPAGATLF
VGNSMPIRDADTFLFATDKPLRVLANRGANGIDGVVSSALGASLAASPLVLVIGDLSFYHDLNGLLAAKMHGLQATIVLM
NNNGGGIFSFLPQARHEGPFETLFGTPTDLTFAHAVEMYGGRYAVPHTWGEFRHHVAESLNTGGLSVIEVRTSRTENVQM
HRFLWERVSQEIAKFLEQKGTEEPWN
>Mature_585_residues
TNALSWYVAAFVDGLVQAGVTEAVISPGSRSTPLAMAMAAHSGLHFSMHIDERSAAFFALGMVKAKQRPVALVCTSGTAA
ANYLPAIVEAYYSRVPLVVLTADRPHELRDVGAPQAIDQLHLYGRYAKWFVDLALPEETDPMLSYARTMAARAAAIAAGA
PAGPVHVNVPLREPLVPTIDEAVWEKVRTVAETPQIMSGRATLPAENVAALYEQLAAAKRGLIVCGALDQPGFAEAVTEL
ARTLDFPILADPLSQLRAGAHDKTYVIDSYDAILKDEAVASRLVPDVVLRFGAMPVSKPLFLWLKQHRSIRQIVVDDGGW
RDPTLEAACFVRSDETVLCRQLLDIARPKQKESAWSTTWREMNDIARTVLRQHLPADEWFEGKVFTELAELLPAGATLFV
GNSMPIRDADTFLFATDKPLRVLANRGANGIDGVVSSALGASLAASPLVLVIGDLSFYHDLNGLLAAKMHGLQATIVLMN
NNGGGIFSFLPQARHEGPFETLFGTPTDLTFAHAVEMYGGRYAVPHTWGEFRHHVAESLNTGGLSVIEVRTSRTENVQMH
RFLWERVSQEIAKFLEQKGTEEPWN

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=583, Percent_Identity=30.3602058319039, Blast_Score=217, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_GEOTN (A4IS14)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001126863.1
- ProteinModelPortal:   A4IS14
- STRING:   A4IS14
- GeneID:   4967699
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_2773
- NMPDR:   fig|420246.5.peg.2674
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- PhylomeDB:   A4IS14
- ProtClustDB:   PRK07449
- BioCyc:   GTHE420246:GTNG_2773-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 63934; Mature: 63803

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNALSWYVAAFVDGLVQAGVTEAVISPGSRSTPLAMAMAAHSGLHFSMHIDERSAAFFA
CCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHH
LGMVKAKQRPVALVCTSGTAAANYLPAIVEAYYSRVPLVVLTADRPHELRDVGAPQAIDQ
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHHH
LHLYGRYAKWFVDLALPEETDPMLSYARTMAARAAAIAAGAPAGPVHVNVPLREPLVPTI
HHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHH
DEAVWEKVRTVAETPQIMSGRATLPAENVAALYEQLAAAKRGLIVCGALDQPGFAEAVTE
HHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHH
LARTLDFPILADPLSQLRAGAHDKTYVIDSYDAILKDEAVASRLVPDVVLRFGAMPVSKP
HHHHCCCCHHHCHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LFLWLKQHRSIRQIVVDDGGWRDPTLEAACFVRSDETVLCRQLLDIARPKQKESAWSTTW
HHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHH
REMNDIARTVLRQHLPADEWFEGKVFTELAELLPAGATLFVGNSMPIRDADTFLFATDKP
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEECCCH
LRVLANRGANGIDGVVSSALGASLAASPLVLVIGDLSFYHDLNGLLAAKMHGLQATIVLM
HHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEEEE
NNNGGGIFSFLPQARHEGPFETLFGTPTDLTFAHAVEMYGGRYAVPHTWGEFRHHVAESL
ECCCCCHHHHCHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHC
NTGGLSVIEVRTSRTENVQMHRFLWERVSQEIAKFLEQKGTEEPWN
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TNALSWYVAAFVDGLVQAGVTEAVISPGSRSTPLAMAMAAHSGLHFSMHIDERSAAFFA
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHH
LGMVKAKQRPVALVCTSGTAAANYLPAIVEAYYSRVPLVVLTADRPHELRDVGAPQAIDQ
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHHH
LHLYGRYAKWFVDLALPEETDPMLSYARTMAARAAAIAAGAPAGPVHVNVPLREPLVPTI
HHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHH
DEAVWEKVRTVAETPQIMSGRATLPAENVAALYEQLAAAKRGLIVCGALDQPGFAEAVTE
HHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHH
LARTLDFPILADPLSQLRAGAHDKTYVIDSYDAILKDEAVASRLVPDVVLRFGAMPVSKP
HHHHCCCCHHHCHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LFLWLKQHRSIRQIVVDDGGWRDPTLEAACFVRSDETVLCRQLLDIARPKQKESAWSTTW
HHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHH
REMNDIARTVLRQHLPADEWFEGKVFTELAELLPAGATLFVGNSMPIRDADTFLFATDKP
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEECCCH
LRVLANRGANGIDGVVSSALGASLAASPLVLVIGDLSFYHDLNGLLAAKMHGLQATIVLM
HHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEEEE
NNNGGGIFSFLPQARHEGPFETLFGTPTDLTFAHAVEMYGGRYAVPHTWGEFRHHVAESL
ECCCCCHHHHCHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHC
NTGGLSVIEVRTSRTENVQMHRFLWERVSQEIAKFLEQKGTEEPWN
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA