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The map label for this gene is amyX [H]

Identifier: 138896368

GI number: 138896368

Start: 2847391

End: 2849571

Strand: Reverse

Name: amyX [H]

Synonym: GTNG_2731

Alternate gene names: 138896368

Gene position: 2849571-2847391 (Counterclockwise)

Preceding gene: 138896369

Following gene: 138896367

Centisome position: 80.26

GC content: 53.28

Gene sequence:

>2181_bases
ATGCTTCATATTAACCGAACGTTTGTCGCCTATTTGGACGAGATGGATCAATTGACCGTGTTTGTACCGAAGTCGTGTTG
CCCTGACGAGATGGCGCCATTTACGATGGTGGCACCGAGCGGAGAGGAAATTCCGCTTTCCGTGCAGCAGCTCGAGGACT
TAGGCGATATGGTGAAATATGTATGTCGATTTGCGTCTGCGTTCGAGTTTGGGGCGACCTATTGGGTTCGTGCCCGTTCG
GGGGAGAGAACCGATGTGCAAATCGGTGCTGTCGTGCGGACGCCGGCGTTCGATGATCAATTTTTTTACGAAGGAAAGCT
AGGAGTGGACTACACGAAGGAGCAAACGGTGTTCCGCGTCTGGGCACCGACGGCAACCGCCGTCAACGTCAAGTTGATCC
ATCCGGATAGCGGTGATGCCCGCTATGTGCCACTTGAGCGAGGGGAGCGCGGTGTATGGTCGGCGGTCGTCCCCGGCGAC
TGGGAACGAACGTATTATACGTATGTTGCCTGCATTAACCGCGTATGGCGCGAGGCAGTGGATCCATATGCGACCGCTGT
TTCCATCAATGGTGAGTATGGCGTGATCATCGACTGGGAAAAGACGAAGCTCGCACTATCCGCTCGTCCGTTGCCGCCGC
TCACATCACCGACTGACGCCATTATTTACGAATTAAGCATCCGTGATTTTACGAGCCACCCCGACAGCGGTGCCGCCCAT
AAAGGGAAGTATCTTGGGTTGTCGGAAGCGAATACAAGTGGGCCGAACGGAACATCGACCGGGCTTTCCTATTTAAAAGA
GCTGGGTGTCACCCATGTGCAGATTATGCCGTTTACCGATTTTGCTGGAGTTGATGAACGCGATCCGTCAGCCTCGTATA
ACTGGGGATACAATCCACTTCATCTATATGCACCGGAAGGAAGTTATGCAACCGATCCGACCGATCCTTACGCGCGCATT
GTTGAATTAAAACAAATGATTCGAACGCTGCACGAAAATGGTTTGCGTGTTGTCATGGATGCGGTATATAACCATGTCTA
CGATCGGGAGCAGTCATCACTGGAGAAGCTTGTTCCCGGGTATTATTTCCGCTATGATGCGTATGGCCAGCCAGCCAACG
GCACCGGCGTCGGCAACGACATCGCTTCTGAGCGGCGGATGGCCCGTCGGTGGATCGTCGATTCGGTCATCTTTTGGGCG
AAAGAGTACGGCATTAATGGATTTCGTTTCGATTTGATGGGTGTGCATGATATAGAGACGATGAAAGTCGTGCGTGACGC
TCTCGATGCAATCGATCCATCGATTCTCGTGTATGGGGAAGGTTGGGACTTGCCGACACCGCTTGCGCCAGAGCAAAAAG
CGACGATGGCCAACGCTTGGAAGTTGCCGCGCATTGCCTATTTCAATGATCGGTTCCGCGATACGATCAAAGGAAGCACG
TTCCATTTGCCAAGCCGCGGCTTTGCACTCGGCGATGCAAGCGGGCGCGAACAGGCGAAAACGGTCATCGCCGGCAGCTT
GCGGGCGTTAGGCGGGCTGTTTTGTCATCCACTCCAATCGTTGAACTATGTAGAATGTCATGATAATCATACTTTTTGGG
ATAAGATGGAAGTTGCGAACGGCTACGAATCAGAAGCGATTAGGCGCAAACGGCAAAAGCTCGCCACGGCGATCGTTCTA
TTGGCGCAAGGGATTCCATTTTTGCATAGCGGTCAGGAGTTTTATCGGACAAAAGGGGGAGATGGCAACAGCTATCAGGC
ACCTGATGAGGTGAATTGGATCGATTGGGAGCAGAAAAGCCGCTATGAAAACGACGTTCGCTATGTTCAAGGGCTGATCG
CTCTTCGTCGGGCGCACGGTGCGTTTCGCCTCGCGACAGAGGCGGAGGTGTTGCGCCATTTCGAGTTTCTTGAACCAACC
CCGCCGTCAGTGATCGCTTATTGGCTGCGCGATGTCGCCGTCTACGGGCCGTGGGGGGACATCATTGTCATTCACCATCA
TGAAGAGAAATCGGAGACAATTGTTCTTCCCGATCAAGAAGAATGGGACGTCGTCTGCGACGGAGAGCAAAGCGGGACGA
TTCCGCTCCGACGGGTGCGCCATGTTGTTGACCTTGACGGTATCGGTACATGGGTGCTTGTCAGAACAGAAGCGATGAAT
GCCGATGAAAATGCGGGATGA

Upstream 100 bases:

>100_bases
CTGTGCATCGATGGGGCGAACCGGCCGATTTTTTGGCACCGCCCTTTTTCTTTTGCGTATGATAGTAGTAACGGGAGGCC
ACAAAGGGGGGTGAGCCAAT

Downstream 100 bases:

>100_bases
GCGGAATCCCAATGGGAAAACTCCTTCCCTTTTGTTTTGCTGTGCAGACTTTGGCAGTTTTATGCAAGTGTCTTGGTAAG
AAAAATGTTCGCATGCCGGG

Product: pullulanase type I

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 726; Mature: 726

Protein sequence:

>726_residues
MLHINRTFVAYLDEMDQLTVFVPKSCCPDEMAPFTMVAPSGEEIPLSVQQLEDLGDMVKYVCRFASAFEFGATYWVRARS
GERTDVQIGAVVRTPAFDDQFFYEGKLGVDYTKEQTVFRVWAPTATAVNVKLIHPDSGDARYVPLERGERGVWSAVVPGD
WERTYYTYVACINRVWREAVDPYATAVSINGEYGVIIDWEKTKLALSARPLPPLTSPTDAIIYELSIRDFTSHPDSGAAH
KGKYLGLSEANTSGPNGTSTGLSYLKELGVTHVQIMPFTDFAGVDERDPSASYNWGYNPLHLYAPEGSYATDPTDPYARI
VELKQMIRTLHENGLRVVMDAVYNHVYDREQSSLEKLVPGYYFRYDAYGQPANGTGVGNDIASERRMARRWIVDSVIFWA
KEYGINGFRFDLMGVHDIETMKVVRDALDAIDPSILVYGEGWDLPTPLAPEQKATMANAWKLPRIAYFNDRFRDTIKGST
FHLPSRGFALGDASGREQAKTVIAGSLRALGGLFCHPLQSLNYVECHDNHTFWDKMEVANGYESEAIRRKRQKLATAIVL
LAQGIPFLHSGQEFYRTKGGDGNSYQAPDEVNWIDWEQKSRYENDVRYVQGLIALRRAHGAFRLATEAEVLRHFEFLEPT
PPSVIAYWLRDVAVYGPWGDIIVIHHHEEKSETIVLPDQEEWDVVCDGEQSGTIPLRRVRHVVDLDGIGTWVLVRTEAMN
ADENAG

Sequences:

>Translated_726_residues
MLHINRTFVAYLDEMDQLTVFVPKSCCPDEMAPFTMVAPSGEEIPLSVQQLEDLGDMVKYVCRFASAFEFGATYWVRARS
GERTDVQIGAVVRTPAFDDQFFYEGKLGVDYTKEQTVFRVWAPTATAVNVKLIHPDSGDARYVPLERGERGVWSAVVPGD
WERTYYTYVACINRVWREAVDPYATAVSINGEYGVIIDWEKTKLALSARPLPPLTSPTDAIIYELSIRDFTSHPDSGAAH
KGKYLGLSEANTSGPNGTSTGLSYLKELGVTHVQIMPFTDFAGVDERDPSASYNWGYNPLHLYAPEGSYATDPTDPYARI
VELKQMIRTLHENGLRVVMDAVYNHVYDREQSSLEKLVPGYYFRYDAYGQPANGTGVGNDIASERRMARRWIVDSVIFWA
KEYGINGFRFDLMGVHDIETMKVVRDALDAIDPSILVYGEGWDLPTPLAPEQKATMANAWKLPRIAYFNDRFRDTIKGST
FHLPSRGFALGDASGREQAKTVIAGSLRALGGLFCHPLQSLNYVECHDNHTFWDKMEVANGYESEAIRRKRQKLATAIVL
LAQGIPFLHSGQEFYRTKGGDGNSYQAPDEVNWIDWEQKSRYENDVRYVQGLIALRRAHGAFRLATEAEVLRHFEFLEPT
PPSVIAYWLRDVAVYGPWGDIIVIHHHEEKSETIVLPDQEEWDVVCDGEQSGTIPLRRVRHVVDLDGIGTWVLVRTEAMN
ADENAG
>Mature_726_residues
MLHINRTFVAYLDEMDQLTVFVPKSCCPDEMAPFTMVAPSGEEIPLSVQQLEDLGDMVKYVCRFASAFEFGATYWVRARS
GERTDVQIGAVVRTPAFDDQFFYEGKLGVDYTKEQTVFRVWAPTATAVNVKLIHPDSGDARYVPLERGERGVWSAVVPGD
WERTYYTYVACINRVWREAVDPYATAVSINGEYGVIIDWEKTKLALSARPLPPLTSPTDAIIYELSIRDFTSHPDSGAAH
KGKYLGLSEANTSGPNGTSTGLSYLKELGVTHVQIMPFTDFAGVDERDPSASYNWGYNPLHLYAPEGSYATDPTDPYARI
VELKQMIRTLHENGLRVVMDAVYNHVYDREQSSLEKLVPGYYFRYDAYGQPANGTGVGNDIASERRMARRWIVDSVIFWA
KEYGINGFRFDLMGVHDIETMKVVRDALDAIDPSILVYGEGWDLPTPLAPEQKATMANAWKLPRIAYFNDRFRDTIKGST
FHLPSRGFALGDASGREQAKTVIAGSLRALGGLFCHPLQSLNYVECHDNHTFWDKMEVANGYESEAIRRKRQKLATAIVL
LAQGIPFLHSGQEFYRTKGGDGNSYQAPDEVNWIDWEQKSRYENDVRYVQGLIALRRAHGAFRLATEAEVLRHFEFLEPT
PPSVIAYWLRDVAVYGPWGDIIVIHHHEEKSETIVLPDQEEWDVVCDGEQSGTIPLRRVRHVVDLDGIGTWVLVRTEAMN
ADENAG

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=451, Percent_Identity=25.9423503325942, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789839, Length=303, Percent_Identity=25.4125412541254, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 81809; Mature: 81809

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHINRTFVAYLDEMDQLTVFVPKSCCPDEMAPFTMVAPSGEEIPLSVQQLEDLGDMVKY
CEEECHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
VCRFASAFEFGATYWVRARSGERTDVQIGAVVRTPAFDDQFFYEGKLGVDYTKEQTVFRV
HHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCCCCEEEEE
WAPTATAVNVKLIHPDSGDARYVPLERGERGVWSAVVPGDWERTYYTYVACINRVWREAV
ECCCCEEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHC
DPYATAVSINGEYGVIIDWEKTKLALSARPLPPLTSPTDAIIYELSIRDFTSHPDSGAAH
CCCEEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
KGKYLGLSEANTSGPNGTSTGLSYLKELGVTHVQIMPFTDFAGVDERDPSASYNWGYNPL
CCCEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCE
HLYAPEGSYATDPTDPYARIVELKQMIRTLHENGLRVVMDAVYNHVYDREQSSLEKLVPG
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCC
YYFRYDAYGQPANGTGVGNDIASERRMARRWIVDSVIFWAKEYGINGFRFDLMGVHDIET
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
MKVVRDALDAIDPSILVYGEGWDLPTPLAPEQKATMANAWKLPRIAYFNDRFRDTIKGST
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHCCCE
FHLPSRGFALGDASGREQAKTVIAGSLRALGGLFCHPLQSLNYVECHDNHTFWDKMEVAN
EECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCC
GYESEAIRRKRQKLATAIVLLAQGIPFLHSGQEFYRTKGGDGNSYQAPDEVNWIDWEQKS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
RYENDVRYVQGLIALRRAHGAFRLATEAEVLRHFEFLEPTPPSVIAYWLRDVAVYGPWGD
HHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCC
IIVIHHHEEKSETIVLPDQEEWDVVCDGEQSGTIPLRRVRHVVDLDGIGTWVLVRTEAMN
EEEEEECCCCCCEEECCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCC
ADENAG
CCCCCC
>Mature Secondary Structure
MLHINRTFVAYLDEMDQLTVFVPKSCCPDEMAPFTMVAPSGEEIPLSVQQLEDLGDMVKY
CEEECHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
VCRFASAFEFGATYWVRARSGERTDVQIGAVVRTPAFDDQFFYEGKLGVDYTKEQTVFRV
HHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCCCCEEEEE
WAPTATAVNVKLIHPDSGDARYVPLERGERGVWSAVVPGDWERTYYTYVACINRVWREAV
ECCCCEEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHC
DPYATAVSINGEYGVIIDWEKTKLALSARPLPPLTSPTDAIIYELSIRDFTSHPDSGAAH
CCCEEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
KGKYLGLSEANTSGPNGTSTGLSYLKELGVTHVQIMPFTDFAGVDERDPSASYNWGYNPL
CCCEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCE
HLYAPEGSYATDPTDPYARIVELKQMIRTLHENGLRVVMDAVYNHVYDREQSSLEKLVPG
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCC
YYFRYDAYGQPANGTGVGNDIASERRMARRWIVDSVIFWAKEYGINGFRFDLMGVHDIET
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
MKVVRDALDAIDPSILVYGEGWDLPTPLAPEQKATMANAWKLPRIAYFNDRFRDTIKGST
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHCCCE
FHLPSRGFALGDASGREQAKTVIAGSLRALGGLFCHPLQSLNYVECHDNHTFWDKMEVAN
EECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCC
GYESEAIRRKRQKLATAIVLLAQGIPFLHSGQEFYRTKGGDGNSYQAPDEVNWIDWEQKS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
RYENDVRYVQGLIALRRAHGAFRLATEAEVLRHFEFLEPTPPSVIAYWLRDVAVYGPWGD
HHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCC
IIVIHHHEEKSETIVLPDQEEWDVVCDGEQSGTIPLRRVRHVVDLDGIGTWVLVRTEAMN
EEEEEECCCCCCEEECCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCC
ADENAG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]