Click here to switch to the map view.

The map label for this gene is mdh [H]

Identifier: 138896295

GI number: 138896295

Start: 2766402

End: 2767340

Strand: Reverse

Name: mdh [H]

Synonym: GTNG_2658

Alternate gene names: 138896295

Gene position: 2767340-2766402 (Counterclockwise)

Preceding gene: 138896296

Following gene: 138896294

Centisome position: 77.95

GC content: 54.53

Gene sequence:

>939_bases
ATGGCGATGAAACGGAAAAAAATTTCGGTCATTGGCGCCGGATTTACGGGAGCGACGACGGCGTTCCTTTTGGCGCAAAA
AGAGCTTGGCGACATCGTGCTTGTCGACATTCCGCAGCTTGAGAACCCGACGAAAGGGAAAGCGCTCGATATGCTCGAGT
CAAGCCCGGTACTCGGCTTTGACGCGAACATCGTCGGTACGTCGGATTACGCTGACACGGCTGATTCTGACATCGTCGTC
ATTACGGCGGGCATCGCCCGCAAGCCGGGAATGAGCCGCGACGACTTAGTGACGACAAACCAAAAAATCATGAAACAAGT
GACGAAAGAAGTCGTCAAATACTCGCCAAACTGCTACATCATCGTCTTGACGAACCCAGTCGATGCGATGACGTATACGG
TCTTTAAGGAATCCGGATTCCCGAAAAACCGCGTCATCGGTCAATCGGGCGTCCTCGATACGGCACGCTTCCGTACGTTC
GTCGCCCAGGAGCTGAACATTTCGGTGAAAGACGTCACCGGGTTTGTGTTGGGCGGCCATGGCGATGACATGGTGCCGCT
CGTCCGCTACTCGTACGCCGGCGGCATTCCGCTTGAAAAACTTATTCCAAAAGACCGTCTTGACGCCATCGTTGAGCGGA
CGCGCAAAGGCGGCGGCGAAATCGTCAACCTGCTTGGCAATGGCAGCGCCTACTATGCGCCGGCTGCCTCGCTTGCCGAA
ATGGTCGAGGCGATTGTCAAAGATCAACGCCGCATTTTGCCGGCGATCGCCTACCTTGAAGGCGAATACGGCTATGAAGG
CATTTATTTGGGGGTGCCAACGATCCTAGGTGGCAACGGCATCGAGAAAGTGATTGAGCTCGAGCTGACCGAGGATGAAA
AAGCGGCACTCGCCAAATCGGTTGAGTCGGTCAAAAACGTCATGCGCGTATTAGAATAA

Upstream 100 bases:

>100_bases
GCCCGTCTGATGGAAGGGGCAACCGAAGTGAAATGTTCGGAATTTGCTGATGCGCTCATCCGCAATATGGACTAACCTTT
AACGAAGGGGATGGCAAACG

Downstream 100 bases:

>100_bases
CCGCAAGGCAAAAATTCGGGCGTTGTCCCGAATTTTTGCACCTTCATTTTGAAAGCGTTTTCCAAATCAAGGGGGTGGGA
ACGTGTTAAAAAAACGGAAG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MAMKRKKISVIGAGFTGATTAFLLAQKELGDIVLVDIPQLENPTKGKALDMLESSPVLGFDANIVGTSDYADTADSDIVV
ITAGIARKPGMSRDDLVTTNQKIMKQVTKEVVKYSPNCYIIVLTNPVDAMTYTVFKESGFPKNRVIGQSGVLDTARFRTF
VAQELNISVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLEKLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLAE
MVEAIVKDQRRILPAIAYLEGEYGYEGIYLGVPTILGGNGIEKVIELELTEDEKAALAKSVESVKNVMRVLE

Sequences:

>Translated_312_residues
MAMKRKKISVIGAGFTGATTAFLLAQKELGDIVLVDIPQLENPTKGKALDMLESSPVLGFDANIVGTSDYADTADSDIVV
ITAGIARKPGMSRDDLVTTNQKIMKQVTKEVVKYSPNCYIIVLTNPVDAMTYTVFKESGFPKNRVIGQSGVLDTARFRTF
VAQELNISVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLEKLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLAE
MVEAIVKDQRRILPAIAYLEGEYGYEGIYLGVPTILGGNGIEKVIELELTEDEKAALAKSVESVKNVMRVLE
>Mature_311_residues
AMKRKKISVIGAGFTGATTAFLLAQKELGDIVLVDIPQLENPTKGKALDMLESSPVLGFDANIVGTSDYADTADSDIVVI
TAGIARKPGMSRDDLVTTNQKIMKQVTKEVVKYSPNCYIIVLTNPVDAMTYTVFKESGFPKNRVIGQSGVLDTARFRTFV
AQELNISVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLEKLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLAEM
VEAIVKDQRRILPAIAYLEGEYGYEGIYLGVPTILGGNGIEKVIELELTEDEKAALAKSVESVKNVMRVLE

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=320, Percent_Identity=39.0625, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI221136809, Length=320, Percent_Identity=39.0625, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI15082234, Length=319, Percent_Identity=37.3040752351097, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI5031857, Length=317, Percent_Identity=37.2239747634069, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI260099723, Length=317, Percent_Identity=37.2239747634069, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI291575128, Length=315, Percent_Identity=36.8253968253968, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI4557032, Length=315, Percent_Identity=36.8253968253968, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI9257228, Length=314, Percent_Identity=34.7133757961783, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI4504973, Length=314, Percent_Identity=34.7133757961783, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI260099727, Length=219, Percent_Identity=39.7260273972603, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=42, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=34.6733668341709, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21735621, Length=296, Percent_Identity=31.4189189189189, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=27.3311897106109, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=29.1208791208791, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789632, Length=324, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=294, Percent_Identity=30.952380952381, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17554310, Length=290, Percent_Identity=30.3448275862069, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322765, Length=294, Percent_Identity=29.5918367346939, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320125, Length=240, Percent_Identity=28.75, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324446, Length=178, Percent_Identity=33.1460674157303, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17136226, Length=311, Percent_Identity=30.8681672025724, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=32.1311475409836, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24647881, Length=314, Percent_Identity=30.2547770700637, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24663599, Length=233, Percent_Identity=32.1888412017167, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24663595, Length=233, Percent_Identity=28.755364806867, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33652; Mature: 33521

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMKRKKISVIGAGFTGATTAFLLAQKELGDIVLVDIPQLENPTKGKALDMLESSPVLGF
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DANIVGTSDYADTADSDIVVITAGIARKPGMSRDDLVTTNQKIMKQVTKEVVKYSPNCYI
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEE
IVLTNPVDAMTYTVFKESGFPKNRVIGQSGVLDTARFRTFVAQELNISVKDVTGFVLGGH
EEEECCCCHHEEEEECCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCEEEEEEEEEEEECC
GDDMVPLVRYSYAGGIPLEKLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLAE
CCCCCHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MVEAIVKDQRRILPAIAYLEGEYGYEGIYLGVPTILGGNGIEKVIELELTEDEKAALAKS
HHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCEECCCCCCEEEEEEECCCHHHHHHHH
VESVKNVMRVLE
HHHHHHHHHHHC
>Mature Secondary Structure 
AMKRKKISVIGAGFTGATTAFLLAQKELGDIVLVDIPQLENPTKGKALDMLESSPVLGF
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DANIVGTSDYADTADSDIVVITAGIARKPGMSRDDLVTTNQKIMKQVTKEVVKYSPNCYI
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEE
IVLTNPVDAMTYTVFKESGFPKNRVIGQSGVLDTARFRTFVAQELNISVKDVTGFVLGGH
EEEECCCCHHEEEEECCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCEEEEEEEEEEEECC
GDDMVPLVRYSYAGGIPLEKLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLAE
CCCCCHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MVEAIVKDQRRILPAIAYLEGEYGYEGIYLGVPTILGGNGIEKVIELELTEDEKAALAKS
HHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCEECCCCCCEEEEEEECCCHHHHHHHH
VESVKNVMRVLE
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA