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The map label for this gene is recN [H]

Identifier: 138895960

GI number: 138895960

Start: 2441579

End: 2443300

Strand: Reverse

Name: recN [H]

Synonym: GTNG_2319

Alternate gene names: 138895960

Gene position: 2443300-2441579 (Counterclockwise)

Preceding gene: 138895961

Following gene: 138895959

Centisome position: 68.82

GC content: 50.52

Gene sequence:

>1722_bases
ATGCTCGCCGAGCTGTCGATTAAAAATTTCGCGATCATTGAATCGCTATCTTTGTCCTTTGACAAAGGACTGACAGTGTT
AACCGGCGAAACAGGGGCTGGTAAATCGATCATCATTGATGCGATTCATCTATTGATCGGCGGGCGCGGTTCCGCCGAAT
TTGTCCGTTTCGGCGCGGAAAAGGCAGAAATCGAAGGGCTGTTTTTGCTTGATGATGACCGCCACCCATGTTGCCAAAAG
TGCGCTGAGGTCGGCATCGATGTGAGCGAAGGAATGGTCGTTTTGCGCCGCGACATTTTGGCTAACGGCAAAAGCGTCTG
CCGCATTAACGGCAAGCTGGTGACAACCGCCATATTGCGGGAAGTTGGAGCGACGCTTGTCGATATTCACGGTCAGCATG
AACATCAAGAGCTGATGGATCCATCGCGTCATTTACCGCTTCTTGATGAATTCGGCGGTGCGGAGATGGCGGAGGCGCTC
GCACGCTACCGCGCTGTCTATGAACAGCATGAAGCGTTGGTGAAAAAATTAAAAAAGCTGAGTGAAAATGAACAGCAAAT
GGCGCATCGACTTGATTTATTGACATTTCAGCTGCGCGAAATCGAACAGGCCACACTCGAGCTCGGAGAAGACGAACGGC
TGATGGAAGAAAAAGTGCGCATTGTCAACTTTCAAAAGATATACAGTGCCATCCAAAAAAGCTATGAAGCGCTCTCCGGT
GAAGGACGCGGCCTTGATTCGATCGGGGAAGCGATGGGCCACCTCGATGATGTCGCCGCGATGGATGCTGCGCTGAAGGA
GGCGTACGAAACAGTTGCCAACAGCTACTACTTGCTTGAAGAAGTGATGTACAAGCTGCGCGACCAGCTCGAGGAGCTTG
AGTATGACCCGGCGCGGCTTGACGCCATCGAAAGCCGCCTCGCTGAAATCCAACAGTTAAAACGAAAATACGGCGCAACG
ATCGCTGATATTTTGCAATATGCTGAGGCGATTGCTGAGGAAATCGAGACGATTGAAAACCGTGAGACGCATGTGCATGA
ACTTGAGCGGCAGCTCGCAGCAGTGACAAGCGATTTGCTGATGGAGGCGGAAAACGTGACCAACGTTCGCCGCACTTACG
CCCGGGAGCTGATTGAACGCATTCACCAAGAGTTAAAAGATTTGTACATGGAGAAGACGCAGTTTGACATCATCTTTGCG
AAACGTGAAGGACCACTCGATGCTCCATTGGTTGATGGCGTGCCGGTTAAGTTTCACGAAGATGGCATCGACGTCGTTGA
GTTTTACATTTCGACGAACGTCGGCGAGCCGTTGAAACCGCTCGCTAAAGTTGCTTCGGGCGGTGAATTGTCACGGATTA
TGTTAGCGTTGAAAACGATTTTTTCCAAACATCAAGGGGTGACGTCGATTATTTTCGACGAGGTGGATACAGGCGTCAGC
GGTCGTGTCGCCCAAGCGATCGCCGAAAAAATTTACCGCATTGCCAGCGGTTCACAAGTGCTTTGCATTTCTCACCTGCC
GCAAGTGGCTGCCATGGCGGACACGCATTTATTCATCGCCAAAGAAACGGACGGAACGCGTACGAAAACAGTGGTGAAAA
AGTTGGATGAGGAGGAGAAGGTAAAAGAAATCGGGCGAATGATTTCCGGCGTCGAAATGACGGAGTTGACAAAACAGCAT
GCCAAAGAACTGTTAGAACTGGCGGCCAAAATGAAACAATAA

Upstream 100 bases:

>100_bases
AAACGGGAACGGTCCTCAATTATGAACGTGAACACATGTTCGATAACAAGAATGAAGATGGACAACATACGCGCCATTCG
AACGTTCAAGGGGTGATGGG

Downstream 100 bases:

>100_bases
CATTTACTTCCAGACCGTGTGCGGTTGCTTGATTTGTGCTGAGTCTTTCCCTCACCAAACCGTCTGATTTGACGGCAGGC
GTTTGATGTGCTGCTGGGAT

Product: DNA repair and genetic recombination

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MLAELSIKNFAIIESLSLSFDKGLTVLTGETGAGKSIIIDAIHLLIGGRGSAEFVRFGAEKAEIEGLFLLDDDRHPCCQK
CAEVGIDVSEGMVVLRRDILANGKSVCRINGKLVTTAILREVGATLVDIHGQHEHQELMDPSRHLPLLDEFGGAEMAEAL
ARYRAVYEQHEALVKKLKKLSENEQQMAHRLDLLTFQLREIEQATLELGEDERLMEEKVRIVNFQKIYSAIQKSYEALSG
EGRGLDSIGEAMGHLDDVAAMDAALKEAYETVANSYYLLEEVMYKLRDQLEELEYDPARLDAIESRLAEIQQLKRKYGAT
IADILQYAEAIAEEIETIENRETHVHELERQLAAVTSDLLMEAENVTNVRRTYARELIERIHQELKDLYMEKTQFDIIFA
KREGPLDAPLVDGVPVKFHEDGIDVVEFYISTNVGEPLKPLAKVASGGELSRIMLALKTIFSKHQGVTSIIFDEVDTGVS
GRVAQAIAEKIYRIASGSQVLCISHLPQVAAMADTHLFIAKETDGTRTKTVVKKLDEEEKVKEIGRMISGVEMTELTKQH
AKELLELAAKMKQ

Sequences:

>Translated_573_residues
MLAELSIKNFAIIESLSLSFDKGLTVLTGETGAGKSIIIDAIHLLIGGRGSAEFVRFGAEKAEIEGLFLLDDDRHPCCQK
CAEVGIDVSEGMVVLRRDILANGKSVCRINGKLVTTAILREVGATLVDIHGQHEHQELMDPSRHLPLLDEFGGAEMAEAL
ARYRAVYEQHEALVKKLKKLSENEQQMAHRLDLLTFQLREIEQATLELGEDERLMEEKVRIVNFQKIYSAIQKSYEALSG
EGRGLDSIGEAMGHLDDVAAMDAALKEAYETVANSYYLLEEVMYKLRDQLEELEYDPARLDAIESRLAEIQQLKRKYGAT
IADILQYAEAIAEEIETIENRETHVHELERQLAAVTSDLLMEAENVTNVRRTYARELIERIHQELKDLYMEKTQFDIIFA
KREGPLDAPLVDGVPVKFHEDGIDVVEFYISTNVGEPLKPLAKVASGGELSRIMLALKTIFSKHQGVTSIIFDEVDTGVS
GRVAQAIAEKIYRIASGSQVLCISHLPQVAAMADTHLFIAKETDGTRTKTVVKKLDEEEKVKEIGRMISGVEMTELTKQH
AKELLELAAKMKQ
>Mature_573_residues
MLAELSIKNFAIIESLSLSFDKGLTVLTGETGAGKSIIIDAIHLLIGGRGSAEFVRFGAEKAEIEGLFLLDDDRHPCCQK
CAEVGIDVSEGMVVLRRDILANGKSVCRINGKLVTTAILREVGATLVDIHGQHEHQELMDPSRHLPLLDEFGGAEMAEAL
ARYRAVYEQHEALVKKLKKLSENEQQMAHRLDLLTFQLREIEQATLELGEDERLMEEKVRIVNFQKIYSAIQKSYEALSG
EGRGLDSIGEAMGHLDDVAAMDAALKEAYETVANSYYLLEEVMYKLRDQLEELEYDPARLDAIESRLAEIQQLKRKYGAT
IADILQYAEAIAEEIETIENRETHVHELERQLAAVTSDLLMEAENVTNVRRTYARELIERIHQELKDLYMEKTQFDIIFA
KREGPLDAPLVDGVPVKFHEDGIDVVEFYISTNVGEPLKPLAKVASGGELSRIMLALKTIFSKHQGVTSIIFDEVDTGVS
GRVAQAIAEKIYRIASGSQVLCISHLPQVAAMADTHLFIAKETDGTRTKTVVKKLDEEEKVKEIGRMISGVEMTELTKQH
AKELLELAAKMKQ

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=574, Percent_Identity=34.4947735191638, Blast_Score=309, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 64000; Mature: 64000

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAELSIKNFAIIESLSLSFDKGLTVLTGETGAGKSIIIDAIHLLIGGRGSAEFVRFGAE
CCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCC
KAEIEGLFLLDDDRHPCCQKCAEVGIDVSEGMVVLRRDILANGKSVCRINGKLVTTAILR
HHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
EVGATLVDIHGQHEHQELMDPSRHLPLLDEFGGAEMAEALARYRAVYEQHEALVKKLKKL
HHCCHHEECCCCCHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SENEQQMAHRLDLLTFQLREIEQATLELGEDERLMEEKVRIVNFQKIYSAIQKSYEALSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGRGLDSIGEAMGHLDDVAAMDAALKEAYETVANSYYLLEEVMYKLRDQLEELEYDPARL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DAIESRLAEIQQLKRKYGATIADILQYAEAIAEEIETIENRETHVHELERQLAAVTSDLL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MEAENVTNVRRTYARELIERIHQELKDLYMEKTQFDIIFAKREGPLDAPLVDGVPVKFHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCEECC
DGIDVVEFYISTNVGEPLKPLAKVASGGELSRIMLALKTIFSKHQGVTSIIFDEVDTGVS
CCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRIASGSQVLCISHLPQVAAMADTHLFIAKETDGTRTKTVVKKLDEEEK
HHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHCCEEEEEECCCCCHHHHHHHHHCHHHH
VKEIGRMISGVEMTELTKQHAKELLELAAKMKQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAELSIKNFAIIESLSLSFDKGLTVLTGETGAGKSIIIDAIHLLIGGRGSAEFVRFGAE
CCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCC
KAEIEGLFLLDDDRHPCCQKCAEVGIDVSEGMVVLRRDILANGKSVCRINGKLVTTAILR
HHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
EVGATLVDIHGQHEHQELMDPSRHLPLLDEFGGAEMAEALARYRAVYEQHEALVKKLKKL
HHCCHHEECCCCCHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SENEQQMAHRLDLLTFQLREIEQATLELGEDERLMEEKVRIVNFQKIYSAIQKSYEALSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGRGLDSIGEAMGHLDDVAAMDAALKEAYETVANSYYLLEEVMYKLRDQLEELEYDPARL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DAIESRLAEIQQLKRKYGATIADILQYAEAIAEEIETIENRETHVHELERQLAAVTSDLL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MEAENVTNVRRTYARELIERIHQELKDLYMEKTQFDIIFAKREGPLDAPLVDGVPVKFHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCEECC
DGIDVVEFYISTNVGEPLKPLAKVASGGELSRIMLALKTIFSKHQGVTSIIFDEVDTGVS
CCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRIASGSQVLCISHLPQVAAMADTHLFIAKETDGTRTKTVVKKLDEEEK
HHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHCCEEEEEECCCCCHHHHHHHHHCHHHH
VKEIGRMISGVEMTELTKQHAKELLELAAKMKQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]