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The map label for this gene is spoIVB [H]

Identifier: 138895959

GI number: 138895959

Start: 2440057

End: 2441352

Strand: Reverse

Name: spoIVB [H]

Synonym: GTNG_2318

Alternate gene names: 138895959

Gene position: 2441352-2440057 (Counterclockwise)

Preceding gene: 138895960

Following gene: 138895958

Centisome position: 68.76

GC content: 48.38

Gene sequence:

>1296_bases
TTGAATAGAGAAACGGCGCGAAAAATGATAGGAGTGCTTCTCCTTGGGATATTGATGGTCATCGGTTTTTCCAAGCCAAT
GAAAGAATATTGGCAAATTCCGAAACAGCTTGTGCTGTTTGAAGGAGACGCGGTCAAGTTTGAAGCCGCCTCTCTTCCGG
TGCAAGCAACCGCCAATGACCGAAACGCTCCCGAAACGCTCGAAGTTGAGCGAAAAAACGGTTCGTTGTCTGTGAAAGCG
AAACAAAGCGGACAGGAAACAATGGTTTTGCAAGTTGCAGGGATGCCGGTTAAGCAAGTGGATGTCAACGTGTTGCCAAC
GCTGAAAGTCGTTCCCGGCGGGCAATCGATCGGTGTGAAACTCAATACGGTCGGTGTTCTTGTCGTTGGTTACCATCTCG
TGGAAACCGAAAACGGAAAAAAATCGCCGGGGGAAGCGGCTGGTATTCAAGTTGGCGATATCATTACCGGCATTAATGGT
AAAAAAATTGAAAACATGAGTGACCTCTCCCCTTTCATTGAGGAAGCTGGCAAAACAGGAAAACCGCTTCATCTGCAAAT
TCTGCGCGACCAGCATTCCATGACAACGAAGCTCGTTCCGCTGAAAGATAAGCATGACGACGTGTACCGCATCGGTTTAT
ACATCCGCGATTCTGCCGCTGGCATCGGGACGATGACATTTTATGACCCGAAATCGAAAAAATACGGCGCACTCGGCCAT
GTCATTTCCGATATGGATACGAAAAAACCGATTGTTGTCCAAGACGGACAAATTATGCGCTCGACCGTTACGTCTATTGA
AAAGGGGACGAGCGGCAATCCAGGGGAAAAATTAGCTCGCTTCTCCGATGGCGGGGAAGTCATTGGTACCATTACGAATA
ACAGTCCGTTTGGCATTTTTGGCAAACTGACAAAATCGTTGCCAGATGGCCCGATTACGAATCCGATTCCGGTCGCATTG
GCGAGTCAGGTGAAAGAAGGGCCGGCGAAAATGTTGACGGTGGTGGAGAATGACAAAGTCGAACAATTTGATGTGGAAAT
TGTTAACACCATTCCGCAAAAATTCCCGGCGACAAAAGGGCTTGTCATTCGCGTCACCGACCCGCGGCTGTTGAAGAAAA
CGGGCGGTATCGTCCAAGGGATGAGCGGCAGTCCAATTATTCAAGATGGAAAGCTCGTTGGTGCAGTGACGCATGTTTTT
GTCAACGATCCGACATCGGGCTATGGTGTCCATATCGAATGGATGTTGCAGGAAGCAGGAATCGATTTATATGGCAAACA
AAGAAAAGCGAGCTGA

Upstream 100 bases:

>100_bases
TTTTTATTCGTGCCGCGATTATATTTTCCCCTGTTCAGGCAAAATTAAAACTGTAACCGTTTTTGTATGGTGTAGGCGGG
AGCGAGGAGAGTGAGCGACA

Downstream 100 bases:

>100_bases
CCGAGTCGGCTCGCTTTTTTGGTCGTTGCAAAATTTTTTTCAAAAAAGGCGGCAAAATTTTTGCTGATTTGGCATAGATA
TTTGGTGAAACATCCGTTAA

Product: stage IV sporulation protein B

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNRETARKMIGVLLLGILMVIGFSKPMKEYWQIPKQLVLFEGDAVKFEAASLPVQATANDRNAPETLEVERKNGSLSVKA
KQSGQETMVLQVAGMPVKQVDVNVLPTLKVVPGGQSIGVKLNTVGVLVVGYHLVETENGKKSPGEAAGIQVGDIITGING
KKIENMSDLSPFIEEAGKTGKPLHLQILRDQHSMTTKLVPLKDKHDDVYRIGLYIRDSAAGIGTMTFYDPKSKKYGALGH
VISDMDTKKPIVVQDGQIMRSTVTSIEKGTSGNPGEKLARFSDGGEVIGTITNNSPFGIFGKLTKSLPDGPITNPIPVAL
ASQVKEGPAKMLTVVENDKVEQFDVEIVNTIPQKFPATKGLVIRVTDPRLLKKTGGIVQGMSGSPIIQDGKLVGAVTHVF
VNDPTSGYGVHIEWMLQEAGIDLYGKQRKAS

Sequences:

>Translated_431_residues
MNRETARKMIGVLLLGILMVIGFSKPMKEYWQIPKQLVLFEGDAVKFEAASLPVQATANDRNAPETLEVERKNGSLSVKA
KQSGQETMVLQVAGMPVKQVDVNVLPTLKVVPGGQSIGVKLNTVGVLVVGYHLVETENGKKSPGEAAGIQVGDIITGING
KKIENMSDLSPFIEEAGKTGKPLHLQILRDQHSMTTKLVPLKDKHDDVYRIGLYIRDSAAGIGTMTFYDPKSKKYGALGH
VISDMDTKKPIVVQDGQIMRSTVTSIEKGTSGNPGEKLARFSDGGEVIGTITNNSPFGIFGKLTKSLPDGPITNPIPVAL
ASQVKEGPAKMLTVVENDKVEQFDVEIVNTIPQKFPATKGLVIRVTDPRLLKKTGGIVQGMSGSPIIQDGKLVGAVTHVF
VNDPTSGYGVHIEWMLQEAGIDLYGKQRKAS
>Mature_431_residues
MNRETARKMIGVLLLGILMVIGFSKPMKEYWQIPKQLVLFEGDAVKFEAASLPVQATANDRNAPETLEVERKNGSLSVKA
KQSGQETMVLQVAGMPVKQVDVNVLPTLKVVPGGQSIGVKLNTVGVLVVGYHLVETENGKKSPGEAAGIQVGDIITGING
KKIENMSDLSPFIEEAGKTGKPLHLQILRDQHSMTTKLVPLKDKHDDVYRIGLYIRDSAAGIGTMTFYDPKSKKYGALGH
VISDMDTKKPIVVQDGQIMRSTVTSIEKGTSGNPGEKLARFSDGGEVIGTITNNSPFGIFGKLTKSLPDGPITNPIPVAL
ASQVKEGPAKMLTVVENDKVEQFDVEIVNTIPQKFPATKGLVIRVTDPRLLKKTGGIVQGMSGSPIIQDGKLVGAVTHVF
VNDPTSGYGVHIEWMLQEAGIDLYGKQRKAS

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 46370; Mature: 46370

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRETARKMIGVLLLGILMVIGFSKPMKEYWQIPKQLVLFEGDAVKFEAASLPVQATAND
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCEEEEECCCCEEECCCC
RNAPETLEVERKNGSLSVKAKQSGQETMVLQVAGMPVKQVDVNVLPTLKVVPGGQSIGVK
CCCCCEEEEEECCCCEEEEECCCCCEEEEEEECCCCCEEECCEECCEEEEECCCCEEEEE
LNTVGVLVVGYHLVETENGKKSPGEAAGIQVGDIITGINGKKIENMSDLSPFIEEAGKTG
EEEEEEEEEEEEEEECCCCCCCCCCCCCEEEHHEEECCCCCCCCCHHHHHHHHHHCCCCC
KPLHLQILRDQHSMTTKLVPLKDKHDDVYRIGLYIRDSAAGIGTMTFYDPKSKKYGALGH
CCEEEEEEECCCCCEEEEEECCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCHHHH
VISDMDTKKPIVVQDGQIMRSTVTSIEKGTSGNPGEKLARFSDGGEVIGTITNNSPFGIF
HHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCHH
GKLTKSLPDGPITNPIPVALASQVKEGPAKMLTVVENDKVEQFDVEIVNTIPQKFPATKG
HHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
LVIRVTDPRLLKKTGGIVQGMSGSPIIQDGKLVGAVTHVFVNDPTSGYGVHIEWMLQEAG
EEEEECCCHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCCEEEEEEEECCC
IDLYGKQRKAS
CCEECCCCCCC
>Mature Secondary Structure
MNRETARKMIGVLLLGILMVIGFSKPMKEYWQIPKQLVLFEGDAVKFEAASLPVQATAND
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCEEEEECCCCEEECCCC
RNAPETLEVERKNGSLSVKAKQSGQETMVLQVAGMPVKQVDVNVLPTLKVVPGGQSIGVK
CCCCCEEEEEECCCCEEEEECCCCCEEEEEEECCCCCEEECCEECCEEEEECCCCEEEEE
LNTVGVLVVGYHLVETENGKKSPGEAAGIQVGDIITGINGKKIENMSDLSPFIEEAGKTG
EEEEEEEEEEEEEEECCCCCCCCCCCCCEEEHHEEECCCCCCCCCHHHHHHHHHHCCCCC
KPLHLQILRDQHSMTTKLVPLKDKHDDVYRIGLYIRDSAAGIGTMTFYDPKSKKYGALGH
CCEEEEEEECCCCCEEEEEECCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCHHHH
VISDMDTKKPIVVQDGQIMRSTVTSIEKGTSGNPGEKLARFSDGGEVIGTITNNSPFGIF
HHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCHH
GKLTKSLPDGPITNPIPVALASQVKEGPAKMLTVVENDKVEQFDVEIVNTIPQKFPATKG
HHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
LVIRVTDPRLLKKTGGIVQGMSGSPIIQDGKLVGAVTHVFVNDPTSGYGVHIEWMLQEAG
EEEEECCCHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCCEEEEEEEECCC
IDLYGKQRKAS
CCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]