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The map label for this gene is ldh [H]

Identifier: 138895951

GI number: 138895951

Start: 2432685

End: 2433785

Strand: Reverse

Name: ldh [H]

Synonym: GTNG_2310

Alternate gene names: 138895951

Gene position: 2433785-2432685 (Counterclockwise)

Preceding gene: 138895952

Following gene: 138895950

Centisome position: 68.55

GC content: 49.77

Gene sequence:

>1101_bases
ATGGAATTGTTTAAATATATGGAAACGTATGATTACGAACAAGTGCTGTTTTGCCAAGATAAAGAATCGGGGTTGAAAGC
GATCATTGCTATTCACGATACGACACTTGGCCCGGCATTGGGTGGTACGCGCATGTGGATGTACAATTCGGAAGAGGAAG
CGCTCGAAGATGCGTTGCGCCTCGCCCGCGGCATGACGTACAAAAACGCGGCAGCCGGTCTTAACTTAGGCGGCGGCAAA
ACGGTCATCATCGGCGATCCACGTAAAGATAAAAATGAAGCGATGTTCCGTGCGTTCGGCCGTTTCATTCAAGGATTGAA
TGGTCGCTATATCACGGCTGAAGACGTCGGTACGACGGTTGCCGATATGGATATCATCTATCAAGAGACAGATTACGTCA
CTGGCATTTCACCGGAGTTCGGTTCATCTGGCAACCCATCGCCAATGACGGCTTACGGTGTTTATCGTGGAATGAAGGCG
GCGGTAAAAGAAGCGTTCGGCAGTGATTCGCTCGAAGGAAAAGTTGTTGCCGTCCAAGGGGTTGGCAATGTCGCCTACCA
CTTGTGCCGTCATTTGCATGAAGAAGGCGCTCAACTTATCGTTACCGACATTAACAAAGAAGCGGTGGCCCGTGCCGTTG
AGGAATTTGGTGCGAAAGCGGTCGACCCGAATGACATTTATGGTGTGGAGTGCGACATTTTTGCTCCGTGCGCACTTGGC
GGTATCATCAATGATCAAACGATTCCGCAACTGAAAGCGAAAGTGATTGCTGGATCGGCGAACAACCAACTGCGCGAGGC
GCGGCATGGCGATATCATCCATGAAATGGGCATCGTTTATGCTCCGGACTATGTGATCAACGCCGGCGGCGTCATTAATG
TCGCTGATGAGCTTTACGGCTACAATCGCGAACGGGCGATGAAAAAAGTGGAACAAATTTACGATAACATTGAAAAAGTG
TTTGCGATCGCTAAGCGTGATAACATTCCGACTTATGTTGCGGCCGATCGGATGGCTGAAGAACGGATCGAAACGATGCG
CAAGTCGCGCAGCCAATTTTTACAAAACGGCCATCATATTTTAAGCCGCCGCAGCCGTTAA

Upstream 100 bases:

>100_bases
ATCGTTTTGACGTCGCGTGCCGACTCGGCGGAAAGCAAATTATATTCGCTCGCTCTTGCGATCTGTTCGGCAACAAAATG
AATGGAGGAGGCCATCAAAG

Downstream 100 bases:

>100_bases
CAGCGGCAAGGGGCGGTTCCCTGCCCCGCTGGCGGCAAACGGAGGGACAAACAACGATGCAAGAGCAGAAGTTCCGTATT
TTAACAATCAATCCAGGTTC

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MELFKYMETYDYEQVLFCQDKESGLKAIIAIHDTTLGPALGGTRMWMYNSEEEALEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEAMFRAFGRFIQGLNGRYITAEDVGTTVADMDIIYQETDYVTGISPEFGSSGNPSPMTAYGVYRGMKA
AVKEAFGSDSLEGKVVAVQGVGNVAYHLCRHLHEEGAQLIVTDINKEAVARAVEEFGAKAVDPNDIYGVECDIFAPCALG
GIINDQTIPQLKAKVIAGSANNQLREARHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERAMKKVEQIYDNIEKV
FAIAKRDNIPTYVAADRMAEERIETMRKSRSQFLQNGHHILSRRSR

Sequences:

>Translated_366_residues
MELFKYMETYDYEQVLFCQDKESGLKAIIAIHDTTLGPALGGTRMWMYNSEEEALEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEAMFRAFGRFIQGLNGRYITAEDVGTTVADMDIIYQETDYVTGISPEFGSSGNPSPMTAYGVYRGMKA
AVKEAFGSDSLEGKVVAVQGVGNVAYHLCRHLHEEGAQLIVTDINKEAVARAVEEFGAKAVDPNDIYGVECDIFAPCALG
GIINDQTIPQLKAKVIAGSANNQLREARHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERAMKKVEQIYDNIEKV
FAIAKRDNIPTYVAADRMAEERIETMRKSRSQFLQNGHHILSRRSR
>Mature_366_residues
MELFKYMETYDYEQVLFCQDKESGLKAIIAIHDTTLGPALGGTRMWMYNSEEEALEDALRLARGMTYKNAAAGLNLGGGK
TVIIGDPRKDKNEAMFRAFGRFIQGLNGRYITAEDVGTTVADMDIIYQETDYVTGISPEFGSSGNPSPMTAYGVYRGMKA
AVKEAFGSDSLEGKVVAVQGVGNVAYHLCRHLHEEGAQLIVTDINKEAVARAVEEFGAKAVDPNDIYGVECDIFAPCALG
GIINDQTIPQLKAKVIAGSANNQLREARHGDIIHEMGIVYAPDYVINAGGVINVADELYGYNRERAMKKVEQIYDNIEKV
FAIAKRDNIPTYVAADRMAEERIETMRKSRSQFLQNGHHILSRRSR

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17544676, Length=312, Percent_Identity=26.2820512820513, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45553475, Length=348, Percent_Identity=26.4367816091954, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45549226, Length=347, Percent_Identity=26.2247838616715, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24649283, Length=296, Percent_Identity=27.3648648648649, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24649460, Length=312, Percent_Identity=25.9615384615385, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 40412; Mature: 40412

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFKYMETYDYEQVLFCQDKESGLKAIIAIHDTTLGPALGGTRMWMYNSEEEALEDALR
CCHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHH
LARGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEAMFRAFGRFIQGLNGRYITAEDVGTTV
HHHCCCCCHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHCCHH
ADMDIIYQETDYVTGISPEFGSSGNPSPMTAYGVYRGMKAAVKEAFGSDSLEGKVVAVQG
HHHHHEEECCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
VGNVAYHLCRHLHEEGAQLIVTDINKEAVARAVEEFGAKAVDPNDIYGVECDIFAPCALG
CCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEECEECHHHHC
GIINDQTIPQLKAKVIAGSANNQLREARHGDIIHEMGIVYAPDYVINAGGVINVADELYG
CCCCCCCHHHHHHHHEECCCCHHHHHHHCCCHHHHCCCEECCCEEEECCCEEEEHHHHHC
YNRERAMKKVEQIYDNIEKVFAIAKRDNIPTYVAADRMAEERIETMRKSRSQFLQNGHHI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LSRRSR
HHCCCC
>Mature Secondary Structure
MELFKYMETYDYEQVLFCQDKESGLKAIIAIHDTTLGPALGGTRMWMYNSEEEALEDALR
CCHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHH
LARGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEAMFRAFGRFIQGLNGRYITAEDVGTTV
HHHCCCCCHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHCCHH
ADMDIIYQETDYVTGISPEFGSSGNPSPMTAYGVYRGMKAAVKEAFGSDSLEGKVVAVQG
HHHHHEEECCCCEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
VGNVAYHLCRHLHEEGAQLIVTDINKEAVARAVEEFGAKAVDPNDIYGVECDIFAPCALG
CCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEECEECHHHHC
GIINDQTIPQLKAKVIAGSANNQLREARHGDIIHEMGIVYAPDYVINAGGVINVADELYG
CCCCCCCHHHHHHHHEECCCCHHHHHHHCCCHHHHCCCEECCCEEEECCCEEEEHHHHHC
YNRERAMKKVEQIYDNIEKVFAIAKRDNIPTYVAADRMAEERIETMRKSRSQFLQNGHHI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LSRRSR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]