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The map label for this gene is buk [H]

Identifier: 138895950

GI number: 138895950

Start: 2431516

End: 2432628

Strand: Reverse

Name: buk [H]

Synonym: GTNG_2309

Alternate gene names: 138895950

Gene position: 2432628-2431516 (Counterclockwise)

Preceding gene: 138895951

Following gene: 138895949

Centisome position: 68.52

GC content: 54.09

Gene sequence:

>1113_bases
ATGCAAGAGCAGAAGTTCCGTATTTTAACAATCAATCCAGGTTCGACCTCAACGAAAATAGGGGTATTTGAAAATGAACG
GCCGCTATTGGAAAAAACGATCCGCCACGATGCGGAGACGCTTCGGCAATATAAGACCATCATCGATCAGTATGAGTTTC
GTAAGCAAACGATTTTAGCCGCTTTAGATGAAGAAGGGATCAACTTATCGAAATTAAGCGCCGTCTGCGGCCGTGGAGGG
CTTCTCCGTCCGATTGAAGGCGGAACGTACCGTGTCAATGAGGCTATGCTTGAAGATTTGCGCCGCGGTTACTCCGGACA
GCATGCGTCCAACCTCGGCGGCATTTTGGCGCATGAGATTGCCTCGGCGTTAAACATTCCGGCGTTTATCGTTGACCCGG
TTGTCGTCGATGAGCTTGAGCCGATCGCCCGGGTCAGCGGCTTTCCACTCATTGAGCGGCGCAGCATTTTCCATGCTTTA
AACCAAAAGGCCGTCGCGCGCCGTGTTGCCCGGCAGCTTGGCAAGCGGTATGATGAGTTGAACTTGATCGTCGCCCATAT
GGGCGGCGGCATTACGGTAGGCGCTCATAAGCAGGGACGGGTTGTCGACGTCAATAACGGTCTCGACGGGGAAGGGCCGT
TCAGTCCGGAACGCGCTGGAACGGTGCCGGCTGGTGATTTGGTCGCTCTATGTTTTTCAGGTGAATATTACCGCGATGAG
ATCATGAGCATGCTTGTCGGCCGCGGCGGTCTTGTCGGCCATCTTGGCACGAACGATGCGGTGAAAGTCGAAAAAATGAT
TGAAGCCGGGGACGAGCAGGCGAAACTGGTGTATGAGGCGATGGCGTACCAAGTGGCAAAAGAAATCGGCGCGGCAAGCG
CGGTGCTAGCTGGCAAAGTTGACGCCATTGTTTTGACCGGTGGACTAGCATACGGCAAATCGTTCGTCGAACAAATTACC
CGCCGTGTCCAATGGATCGCTGATGTTATCGTCCACCCGGGTGAAAATGAACTGCAGGCGCTCGCTGAAGGCGCATTACG
CGTCTTGTGTGGCGAGGAGGAGGAAAAAACGTATCTAGGTGAAGCGGCTTCGCCGGTTTTAGCCCGGCGTTGA

Upstream 100 bases:

>100_bases
TTTTACAAAACGGCCATCATATTTTAAGCCGCCGCAGCCGTTAACAGCGGCAAGGGGCGGTTCCCTGCCCCGCTGGCGGC
AAACGGAGGGACAAACAACG

Downstream 100 bases:

>100_bases
TGCGGCAACGGACAGAAAGGGGAAGGCAAGATGGCAAAAGAATACGATGTTGTCATTCTCGGCGGAGGCACGGGCGGCTA
TGTGGCGGCCATTCGCGCAT

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MQEQKFRILTINPGSTSTKIGVFENERPLLEKTIRHDAETLRQYKTIIDQYEFRKQTILAALDEEGINLSKLSAVCGRGG
LLRPIEGGTYRVNEAMLEDLRRGYSGQHASNLGGILAHEIASALNIPAFIVDPVVVDELEPIARVSGFPLIERRSIFHAL
NQKAVARRVARQLGKRYDELNLIVAHMGGGITVGAHKQGRVVDVNNGLDGEGPFSPERAGTVPAGDLVALCFSGEYYRDE
IMSMLVGRGGLVGHLGTNDAVKVEKMIEAGDEQAKLVYEAMAYQVAKEIGAASAVLAGKVDAIVLTGGLAYGKSFVEQIT
RRVQWIADVIVHPGENELQALAEGALRVLCGEEEEKTYLGEAASPVLARR

Sequences:

>Translated_370_residues
MQEQKFRILTINPGSTSTKIGVFENERPLLEKTIRHDAETLRQYKTIIDQYEFRKQTILAALDEEGINLSKLSAVCGRGG
LLRPIEGGTYRVNEAMLEDLRRGYSGQHASNLGGILAHEIASALNIPAFIVDPVVVDELEPIARVSGFPLIERRSIFHAL
NQKAVARRVARQLGKRYDELNLIVAHMGGGITVGAHKQGRVVDVNNGLDGEGPFSPERAGTVPAGDLVALCFSGEYYRDE
IMSMLVGRGGLVGHLGTNDAVKVEKMIEAGDEQAKLVYEAMAYQVAKEIGAASAVLAGKVDAIVLTGGLAYGKSFVEQIT
RRVQWIADVIVHPGENELQALAEGALRVLCGEEEEKTYLGEAASPVLARR
>Mature_370_residues
MQEQKFRILTINPGSTSTKIGVFENERPLLEKTIRHDAETLRQYKTIIDQYEFRKQTILAALDEEGINLSKLSAVCGRGG
LLRPIEGGTYRVNEAMLEDLRRGYSGQHASNLGGILAHEIASALNIPAFIVDPVVVDELEPIARVSGFPLIERRSIFHAL
NQKAVARRVARQLGKRYDELNLIVAHMGGGITVGAHKQGRVVDVNNGLDGEGPFSPERAGTVPAGDLVALCFSGEYYRDE
IMSMLVGRGGLVGHLGTNDAVKVEKMIEAGDEQAKLVYEAMAYQVAKEIGAASAVLAGKVDAIVLTGGLAYGKSFVEQIT
RRVQWIADVIVHPGENELQALAEGALRVLCGEEEEKTYLGEAASPVLARR

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=170, Percent_Identity=31.7647058823529, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693184, Length=337, Percent_Identity=23.7388724035608, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 40170; Mature: 40170

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEQKFRILTINPGSTSTKIGVFENERPLLEKTIRHDAETLRQYKTIIDQYEFRKQTILA
CCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDEEGINLSKLSAVCGRGGLLRPIEGGTYRVNEAMLEDLRRGYSGQHASNLGGILAHEI
HHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ASALNIPAFIVDPVVVDELEPIARVSGFPLIERRSIFHALNQKAVARRVARQLGKRYDEL
HHHHCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIVAHMGGGITVGAHKQGRVVDVNNGLDGEGPFSPERAGTVPAGDLVALCFSGEYYRDE
HEEEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCHHHHH
IMSMLVGRGGLVGHLGTNDAVKVEKMIEAGDEQAKLVYEAMAYQVAKEIGAASAVLAGKV
HHHHHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCC
DAIVLTGGLAYGKSFVEQITRRVQWIADVIVHPGENELQALAEGALRVLCGEEEEKTYLG
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHC
EAASPVLARR
CCCCCHHCCC
>Mature Secondary Structure
MQEQKFRILTINPGSTSTKIGVFENERPLLEKTIRHDAETLRQYKTIIDQYEFRKQTILA
CCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALDEEGINLSKLSAVCGRGGLLRPIEGGTYRVNEAMLEDLRRGYSGQHASNLGGILAHEI
HHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ASALNIPAFIVDPVVVDELEPIARVSGFPLIERRSIFHALNQKAVARRVARQLGKRYDEL
HHHHCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIVAHMGGGITVGAHKQGRVVDVNNGLDGEGPFSPERAGTVPAGDLVALCFSGEYYRDE
HEEEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCHHHHH
IMSMLVGRGGLVGHLGTNDAVKVEKMIEAGDEQAKLVYEAMAYQVAKEIGAASAVLAGKV
HHHHHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCC
DAIVLTGGLAYGKSFVEQITRRVQWIADVIVHPGENELQALAEGALRVLCGEEEEKTYLG
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHC
EAASPVLARR
CCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA