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The map label for this gene is dacF [H]

Identifier: 138895883

GI number: 138895883

Start: 2359561

End: 2360736

Strand: Reverse

Name: dacF [H]

Synonym: GTNG_2242

Alternate gene names: 138895883

Gene position: 2360736-2359561 (Counterclockwise)

Preceding gene: 138895884

Following gene: 138895882

Centisome position: 66.49

GC content: 49.4

Gene sequence:

>1176_bases
ATGAAAACGACAGTCATCCGTCTGCTTTTATTGCCGCTTCTTCTTTTCCTTCCCCTTTCTGTATATGCTGAGGAGAAAGA
AACAAAGGGGACAAAGACTGAGTTGGCTGATGAGGCGAAGTCGGCCATCTTGATTGAACGCGACACCGGCAAGGTTCTAT
ATGAAAAAAATCCTCATGAAAAACTGCCGCCGGCGAGCATGACAAAAATTATGACGATGTTGTTGATCATGGAGGCGATC
GACGAAGGAAAGCTCTCCTACGATGAGAAAGTACGGACAAGCGAATACGCTGCTTCGATGGGCGGATCGCAAATTTTTCT
CGAGCCAGGCGAGGAAATGACGGTTGATGAATTGCTGCGTGCCATCGCCATCGGTTCAGCGAATGACGCTTCTGTCGCCA
TGGCTGAGCGGATTGCCGGATCGGAAGAAGCGTTTGTTGAAATGATGAACGAAAAGGCGAAAGAACTTGGTTTAAAGGAA
ACGAAATTCGCCAATACGACCGGCCTGCCGGCTGAAGGGCATTACAGCAGCGCCTATGACATGGCCATCATGGCGCGCGA
ACTGTTGAAATACGAGGGAATTACAAAATATACAAGCAAGTACGAAGACTATTTGCGCGAAAACACCGATAAAAAGTTTT
GGCTTGTGAATACTAACCGGCTTGTCAAGTTTTATCCAGGCGTTGACGGGCTGAAAACCGGCTTTACAAGCGAAGCAGGA
TATTGTTTGACGGCGACGGCGAAAAAGAACGGCATGCGCGTCATTGCCGTTGTGTTTGGAGCGCCGACACCAAAATCGCG
CAACGCGCAAATTACCAAAATGCTCGATTACGCGTTTCATCACTATCAAGCCCATCCGGTATACAAGCGCAATGAAACAG
TCGCCCGTGTCGATGTCAGCAAGGGGAAACAAAAGTCGGTCGCAGCCGTAACGTCGGAGCCGGTGTCGGTGCTGACGAAA
AAAGGGCAGTCAGTCGAGCAAATCGAGAAAGTGGTTAAAGTGAAAGATGGCGTGAAAGCGCCGGTTCGAAAAGGCGATGA
GCTTGGCGTCCTTATTTTGAAGCAGGATGGAAAAGAGATTTTGCGCAGCCCGCTTGTTGCCAAACAAACGGTGGCAGAGG
CCAGCTTTTGGGATTTGTTTAAGCGAGTGTTCGGCCGTTTCGTTCAGGCTGGGTAA

Upstream 100 bases:

>100_bases
GCTCGAAACATTCGTTTCGAGCGTTTTTTATTTTGGTTCATTTGTATAAAAATATCTCCATTGGAAAAAATGGCATGTAT
AAAGAATGGAGGTTTGGGAG

Downstream 100 bases:

>100_bases
TGTCAATTTTTGTGATCTTTTCACTCTCTTTGGCGAACGGCCACTAGTTTTGTCGGGCGGCAGGATTTCCGGAGGGCGAC
AGCGAAACTTCTCCGTATCC

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKTTVIRLLLLPLLLFLPLSVYAEEKETKGTKTELADEAKSAILIERDTGKVLYEKNPHEKLPPASMTKIMTMLLIMEAI
DEGKLSYDEKVRTSEYAASMGGSQIFLEPGEEMTVDELLRAIAIGSANDASVAMAERIAGSEEAFVEMMNEKAKELGLKE
TKFANTTGLPAEGHYSSAYDMAIMARELLKYEGITKYTSKYEDYLRENTDKKFWLVNTNRLVKFYPGVDGLKTGFTSEAG
YCLTATAKKNGMRVIAVVFGAPTPKSRNAQITKMLDYAFHHYQAHPVYKRNETVARVDVSKGKQKSVAAVTSEPVSVLTK
KGQSVEQIEKVVKVKDGVKAPVRKGDELGVLILKQDGKEILRSPLVAKQTVAEASFWDLFKRVFGRFVQAG

Sequences:

>Translated_391_residues
MKTTVIRLLLLPLLLFLPLSVYAEEKETKGTKTELADEAKSAILIERDTGKVLYEKNPHEKLPPASMTKIMTMLLIMEAI
DEGKLSYDEKVRTSEYAASMGGSQIFLEPGEEMTVDELLRAIAIGSANDASVAMAERIAGSEEAFVEMMNEKAKELGLKE
TKFANTTGLPAEGHYSSAYDMAIMARELLKYEGITKYTSKYEDYLRENTDKKFWLVNTNRLVKFYPGVDGLKTGFTSEAG
YCLTATAKKNGMRVIAVVFGAPTPKSRNAQITKMLDYAFHHYQAHPVYKRNETVARVDVSKGKQKSVAAVTSEPVSVLTK
KGQSVEQIEKVVKVKDGVKAPVRKGDELGVLILKQDGKEILRSPLVAKQTVAEASFWDLFKRVFGRFVQAG
>Mature_391_residues
MKTTVIRLLLLPLLLFLPLSVYAEEKETKGTKTELADEAKSAILIERDTGKVLYEKNPHEKLPPASMTKIMTMLLIMEAI
DEGKLSYDEKVRTSEYAASMGGSQIFLEPGEEMTVDELLRAIAIGSANDASVAMAERIAGSEEAFVEMMNEKAKELGLKE
TKFANTTGLPAEGHYSSAYDMAIMARELLKYEGITKYTSKYEDYLRENTDKKFWLVNTNRLVKFYPGVDGLKTGFTSEAG
YCLTATAKKNGMRVIAVVFGAPTPKSRNAQITKMLDYAFHHYQAHPVYKRNETVARVDVSKGKQKSVAAVTSEPVSVLTK
KGQSVEQIEKVVKVKDGVKAPVRKGDELGVLILKQDGKEILRSPLVAKQTVAEASFWDLFKRVFGRFVQAG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=394, Percent_Identity=32.741116751269, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786851, Length=347, Percent_Identity=34.8703170028818, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI87082022, Length=363, Percent_Identity=31.404958677686, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI87082054, Length=263, Percent_Identity=28.8973384030418, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43388; Mature: 43388

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTVIRLLLLPLLLFLPLSVYAEEKETKGTKTELADEAKSAILIERDTGKVLYEKNPHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEEECCCCCEEECCCCCC
KLPPASMTKIMTMLLIMEAIDEGKLSYDEKVRTSEYAASMGGSQIFLEPGEEMTVDELLR
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
AIAIGSANDASVAMAERIAGSEEAFVEMMNEKAKELGLKETKFANTTGLPAEGHYSSAYD
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHH
MAIMARELLKYEGITKYTSKYEDYLRENTDKKFWLVNTNRLVKFYPGVDGLKTGFTSEAG
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCHHCCCCCCCC
YCLTATAKKNGMRVIAVVFGAPTPKSRNAQITKMLDYAFHHYQAHPVYKRNETVARVDVS
EEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
KGKQKSVAAVTSEPVSVLTKKGQSVEQIEKVVKVKDGVKAPVRKGDELGVLILKQDGKEI
CCCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHCCCCCEEEEEECCCCHHH
LRSPLVAKQTVAEASFWDLFKRVFGRFVQAG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTTVIRLLLLPLLLFLPLSVYAEEKETKGTKTELADEAKSAILIERDTGKVLYEKNPHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEEECCCCCEEECCCCCC
KLPPASMTKIMTMLLIMEAIDEGKLSYDEKVRTSEYAASMGGSQIFLEPGEEMTVDELLR
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
AIAIGSANDASVAMAERIAGSEEAFVEMMNEKAKELGLKETKFANTTGLPAEGHYSSAYD
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHH
MAIMARELLKYEGITKYTSKYEDYLRENTDKKFWLVNTNRLVKFYPGVDGLKTGFTSEAG
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCHHCCCCCCCC
YCLTATAKKNGMRVIAVVFGAPTPKSRNAQITKMLDYAFHHYQAHPVYKRNETVARVDVS
EEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
KGKQKSVAAVTSEPVSVLTKKGQSVEQIEKVVKVKDGVKAPVRKGDELGVLILKQDGKEI
CCCCHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHCCCCCEEEEEECCCCHHH
LRSPLVAKQTVAEASFWDLFKRVFGRFVQAG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]