Click here to switch to the map view.

The map label for this gene is cobS

Identifier: 138895834

GI number: 138895834

Start: 2316460

End: 2317248

Strand: Reverse

Name: cobS

Synonym: GTNG_2193

Alternate gene names: 138895834

Gene position: 2317248-2316460 (Counterclockwise)

Preceding gene: 138895835

Following gene: 138895833

Centisome position: 65.27

GC content: 55.13

Gene sequence:

>789_bases
ATGAGGCAGATGTGGAACGGCTGGCTGTTGGCGTTGCAGTTGTTTACCGTCATTCCAATTCGGCGATCAATTGAATGGAA
TGACATCCATGTCCGTTGGCTCGTGCGCAGTATGCCGCTTGCGGGGGCGGCGATCGGGGCGCTGGCTGCTGGGACATATG
CTTTATGTTCTATGTTTTCGTTCGGCACGCCGCTCTTTTTGGCGCTTTTTCTTCTTTGGCTCGGTATTTGGCTGGCTGGC
GGTTTGCACGCCGACGGCTGGATGGATGTGAGCGACGCCTTTTTTTCGTATCGCGACGCCAAACGGCGGCAACAGATTAT
GTCCGATTCGCGTGTCGGCGCATTCGCTGTTTTGTCGCTTGCCTGTTTATTGTCATTTCGTTGGTTGTTTTTGTATGAGA
CGATTAAGGCCGAGATTCCGCCAGCTTTGTTTGTTGCGATTCCGCTTTTATCGCGTTCGGGGGCCGCTTGGCTGCTTTCT
GTCGGCAAGCTGGCGAAATCGACAGGAATGGCGGCATCAGTGCGCGAATATATTTCATGGCGCGATGCTGTCTGGGCGTT
GGTGCTCGCTTTCCTCGCCCTTTCACTTCTCTTGGTGTTCGGGGGCGTCCCTGTATGGACAAGTGCAGCGCTCGCTGTGG
CGATGGCGCTTTTGGCGTTAGGGGCCAAGCCATGGGTGGAAAAACAGTTTGGCGGCGTTACCGGCGATGTGCTCGGAGCG
CTGATTGAAGGAGGGGAGACGCTTTTATGGGGCGTCGTTTGGCTATTACATTCATCCGCCATGGGATGA

Upstream 100 bases:

>100_bases
AAACGCTCGGTTGGCTTCATCAACAGTTGGTTGCAGAAGCGGCGGTTGCCGCGGTGGTTGAATGCGGTCTCGTTCGAGTG
AAAAAAGGGCGGTGGCCGCA

Downstream 100 bases:

>100_bases
CAGCGTTGAACCGCCAGCGGGCGTATATTGGTTGGACGGATGCGCCGTTGGCTGCTGCTGAGCGCGTTCGGCTCAGCAGC
CTAAAGCCCAAGCTGCCTAT

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRQMWNGWLLALQLFTVIPIRRSIEWNDIHVRWLVRSMPLAGAAIGALAAGTYALCSMFSFGTPLFLALFLLWLGIWLAG
GLHADGWMDVSDAFFSYRDAKRRQQIMSDSRVGAFAVLSLACLLSFRWLFLYETIKAEIPPALFVAIPLLSRSGAAWLLS
VGKLAKSTGMAASVREYISWRDAVWALVLAFLALSLLLVFGGVPVWTSAALAVAMALLALGAKPWVEKQFGGVTGDVLGA
LIEGGETLLWGVVWLLHSSAMG

Sequences:

>Translated_262_residues
MRQMWNGWLLALQLFTVIPIRRSIEWNDIHVRWLVRSMPLAGAAIGALAAGTYALCSMFSFGTPLFLALFLLWLGIWLAG
GLHADGWMDVSDAFFSYRDAKRRQQIMSDSRVGAFAVLSLACLLSFRWLFLYETIKAEIPPALFVAIPLLSRSGAAWLLS
VGKLAKSTGMAASVREYISWRDAVWALVLAFLALSLLLVFGGVPVWTSAALAVAMALLALGAKPWVEKQFGGVTGDVLGA
LIEGGETLLWGVVWLLHSSAMG
>Mature_262_residues
MRQMWNGWLLALQLFTVIPIRRSIEWNDIHVRWLVRSMPLAGAAIGALAAGTYALCSMFSFGTPLFLALFLLWLGIWLAG
GLHADGWMDVSDAFFSYRDAKRRQQIMSDSRVGAFAVLSLACLLSFRWLFLYETIKAEIPPALFVAIPLLSRSGAAWLLS
VGKLAKSTGMAASVREYISWRDAVWALVLAFLALSLLLVFGGVPVWTSAALAVAMALLALGAKPWVEKQFGGVTGDVLGA
LIEGGETLLWGVVWLLHSSAMG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=240, Percent_Identity=31.6666666666667, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_GEOTN (A4IQD8)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001126287.1
- STRING:   A4IQD8
- GeneID:   4967120
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_2193
- NMPDR:   fig|420246.5.peg.2101
- eggNOG:   COG0368
- HOGENOM:   HBG534265
- OMA:   ELTECAV
- ProtClustDB:   CLSK695243
- BioCyc:   GTHE420246:GTNG_2193-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805

Pfam domain/function: PF02654 CobS

EC number: 2.-.-.- [C]

Molecular weight: Translated: 28575; Mature: 28575

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdfed39c)-; HASH(0xf432888)-; HASH(0xee36d14)-; HASH(0xc211728)-; HASH(0xfd3b294)-; HASH(0xf4ff0b4)-; HASH(0x1025aa4c)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHHHHHHHHHHHHCCH
>Mature Secondary Structure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HHHCCHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA