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The map label for this gene is cca

Identifier: 138895759

GI number: 138895759

Start: 2248694

End: 2249908

Strand: Reverse

Name: cca

Synonym: GTNG_2115

Alternate gene names: 138895759

Gene position: 2249908-2248694 (Counterclockwise)

Preceding gene: 138895760

Following gene: 138895758

Centisome position: 63.37

GC content: 57.94

Gene sequence:

>1215_bases
ATGAAACCGCCATTCCAACAGGCGCTTGGTATCATCCGACAGCTAAATCGGCATGGCTATGAGGCATATTTTGTCGGCGG
CGCGGTGCGCGATCTGTTGCTTGGGCGTCCGATCGGCGATGTCGATATTGCGACGAGCGCCTTGCCGGACGAAGTGATGG
CGATTTTCCCAAAAACGATTGATGTCGGCTCGAAACACGGTACAGTCGTCGTCGTGCACGAAGGAACGGCCTACGAAGTA
ACGACGTTCCGCACCGACGGCGACTATGAAGACCACCGCCGTCCTGAGTCGGTGACGTTCGTTCGTTCTCTTGAGGAAGA
CTTAAAACGGCGCGATTTTACGATGAATGCCATCGCCATGGACGAGCACGGGACAATCATTGACCCGTTCGGGGGACAAG
AGGCGATCGAAAAGCGGCTTATTCGCACTGTCGGAGAAGCGGACGCGCGGTTTCGTGAAGATGCGCTCCGCATGATGCGG
GCCGTCCGCTTTGTCAGTCAACTTGGATTTTCACTCGCTATCGACACGAAGAAGGCGATCATCGCCAACGCACCGCTTCT
CGCCCATATTTCGGTTGAGCGGATGACGATGGAGATGGAAAAGCTGCTCGCCGGCCCGTTTGCTGCCGAAGCGTTGCCGC
TTTTGGCCGAAACAGGGCTATCCACCTATTTGCCGGGGTTGGCTGAAAAAGGCGAGTGGCTGCGACGAGCGGCCGCGTAC
CGCTGGCCTTGGCTCGCGGCGCGCGAGGAGCGGTGGGCGCTTTTGTGCCACGCCCTTGGCGTCAAGGAGAGCCGTCCGTT
TTTGCGCGCATGGAAGCTACCGAATAAAGTGATCGATGAAGCTGGCGCCATTTTGGCGACGCTCGCTGCTGTCCCTGAAC
CGGCCGCGTGGACGAATGAGCAGCTGTTTTTGGCCGGCCTCAAGCGGGCACTTTCGGTTGAGGTGGTCCGCGCTGCTTTG
ACCGGAAAGCCGTACGAACCGCAACATGACGAGCTGCGTCGCCGCTTTGCCGCCTTGCCGATCAAAACGAAAGGTGAGCT
GGCGGTTAACGGCAAAGTGGTCATTGATTGGATCGGGAAACCAGCCGGTCCGTGGGTGAAAGAGACGCTGGATGCCATAT
GGCGGGCGGTCGTCAACGGTGAAGTTGAAAACGAGAAGGAGCGGATTTACGCATGGCTCATGGAGCGCAGTCGGACACAA
GAAAAAAACTGTTAG

Upstream 100 bases:

>100_bases
AAGAGGCGGTGCGAACGGTCGAGCAAAAGTTCCGTTCGTCCGATATCGTCGGGCAGTATGAGCAGCTGTATGCTTCACTG
GCAGCAAGGAAGGTGAGAGG

Downstream 100 bases:

>100_bases
AACTATTTGCCGAGGCAAACGGTGAATTTCTGTCCGGACAAAAGATCAGTGAGCAGCTCGGTTGTTCGCGGGCGGCCGTA
TGGAAACATATCGAGGAGCT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKPPFQQALGIIRQLNRHGYEAYFVGGAVRDLLLGRPIGDVDIATSALPDEVMAIFPKTIDVGSKHGTVVVVHEGTAYEV
TTFRTDGDYEDHRRPESVTFVRSLEEDLKRRDFTMNAIAMDEHGTIIDPFGGQEAIEKRLIRTVGEADARFREDALRMMR
AVRFVSQLGFSLAIDTKKAIIANAPLLAHISVERMTMEMEKLLAGPFAAEALPLLAETGLSTYLPGLAEKGEWLRRAAAY
RWPWLAAREERWALLCHALGVKESRPFLRAWKLPNKVIDEAGAILATLAAVPEPAAWTNEQLFLAGLKRALSVEVVRAAL
TGKPYEPQHDELRRRFAALPIKTKGELAVNGKVVIDWIGKPAGPWVKETLDAIWRAVVNGEVENEKERIYAWLMERSRTQ
EKNC

Sequences:

>Translated_404_residues
MKPPFQQALGIIRQLNRHGYEAYFVGGAVRDLLLGRPIGDVDIATSALPDEVMAIFPKTIDVGSKHGTVVVVHEGTAYEV
TTFRTDGDYEDHRRPESVTFVRSLEEDLKRRDFTMNAIAMDEHGTIIDPFGGQEAIEKRLIRTVGEADARFREDALRMMR
AVRFVSQLGFSLAIDTKKAIIANAPLLAHISVERMTMEMEKLLAGPFAAEALPLLAETGLSTYLPGLAEKGEWLRRAAAY
RWPWLAAREERWALLCHALGVKESRPFLRAWKLPNKVIDEAGAILATLAAVPEPAAWTNEQLFLAGLKRALSVEVVRAAL
TGKPYEPQHDELRRRFAALPIKTKGELAVNGKVVIDWIGKPAGPWVKETLDAIWRAVVNGEVENEKERIYAWLMERSRTQ
EKNC
>Mature_404_residues
MKPPFQQALGIIRQLNRHGYEAYFVGGAVRDLLLGRPIGDVDIATSALPDEVMAIFPKTIDVGSKHGTVVVVHEGTAYEV
TTFRTDGDYEDHRRPESVTFVRSLEEDLKRRDFTMNAIAMDEHGTIIDPFGGQEAIEKRLIRTVGEADARFREDALRMMR
AVRFVSQLGFSLAIDTKKAIIANAPLLAHISVERMTMEMEKLLAGPFAAEALPLLAETGLSTYLPGLAEKGEWLRRAAAY
RWPWLAAREERWALLCHALGVKESRPFLRAWKLPNKVIDEAGAILATLAAVPEPAAWTNEQLFLAGLKRALSVEVVRAAL
TGKPYEPQHDELRRRFAALPIKTKGELAVNGKVVIDWIGKPAGPWVKETLDAIWRAVVNGEVENEKERIYAWLMERSRTQ
EKNC

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=216, Percent_Identity=33.7962962962963, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI87081691, Length=226, Percent_Identity=36.7256637168142, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789436, Length=197, Percent_Identity=35.5329949238579, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=337, Percent_Identity=31.4540059347181, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281360112, Length=220, Percent_Identity=36.3636363636364, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21357337, Length=220, Percent_Identity=36.3636363636364, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_GEOTN (A4IQ63)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001126212.1
- ProteinModelPortal:   A4IQ63
- SMR:   A4IQ63
- STRING:   A4IQ63
- GeneID:   4967043
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_2115
- NMPDR:   fig|420246.5.peg.2022
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   DHGNGYE
- ProtClustDB:   PRK13299
- BioCyc:   GTHE420246:GTNG_2115-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45227; Mature: 45227

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 30-30 BINDING 111-111 BINDING 154-154 BINDING 157-157 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPFQQALGIIRQLNRHGYEAYFVGGAVRDLLLGRPIGDVDIATSALPDEVMAIFPKTI
CCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHCCHH
DVGSKHGTVVVVHEGTAYEVTTFRTDGDYEDHRRPESVTFVRSLEEDLKRRDFTMNAIAM
CCCCCCCEEEEEECCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEE
DEHGTIIDPFGGQEAIEKRLIRTVGEADARFREDALRMMRAVRFVSQLGFSLAIDTKKAI
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHH
IANAPLLAHISVERMTMEMEKLLAGPFAAEALPLLAETGLSTYLPGLAEKGEWLRRAAAY
HCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHH
RWPWLAAREERWALLCHALGVKESRPFLRAWKLPNKVIDEAGAILATLAAVPEPAAWTNE
CCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
QLFLAGLKRALSVEVVRAALTGKPYEPQHDELRRRFAALPIKTKGELAVNGKVVIDWIGK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCEEEEEECCC
PAGPWVKETLDAIWRAVVNGEVENEKERIYAWLMERSRTQEKNC
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPPFQQALGIIRQLNRHGYEAYFVGGAVRDLLLGRPIGDVDIATSALPDEVMAIFPKTI
CCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHCCHH
DVGSKHGTVVVVHEGTAYEVTTFRTDGDYEDHRRPESVTFVRSLEEDLKRRDFTMNAIAM
CCCCCCCEEEEEECCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEE
DEHGTIIDPFGGQEAIEKRLIRTVGEADARFREDALRMMRAVRFVSQLGFSLAIDTKKAI
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHH
IANAPLLAHISVERMTMEMEKLLAGPFAAEALPLLAETGLSTYLPGLAEKGEWLRRAAAY
HCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHH
RWPWLAAREERWALLCHALGVKESRPFLRAWKLPNKVIDEAGAILATLAAVPEPAAWTNE
CCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
QLFLAGLKRALSVEVVRAALTGKPYEPQHDELRRRFAALPIKTKGELAVNGKVVIDWIGK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCEEEEEECCC
PAGPWVKETLDAIWRAVVNGEVENEKERIYAWLMERSRTQEKNC
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA