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The map label for this gene is birA [H]

Identifier: 138895758

GI number: 138895758

Start: 2247747

End: 2248736

Strand: Reverse

Name: birA [H]

Synonym: GTNG_2114

Alternate gene names: 138895758

Gene position: 2248736-2247747 (Counterclockwise)

Preceding gene: 138895759

Following gene: 138895757

Centisome position: 63.34

GC content: 53.13

Gene sequence:

>990_bases
ATGGCTCATGGAGCGCAGTCGGACACAAGAAAAAAACTGTTAGAACTATTTGCCGAGGCAAACGGTGAATTTCTGTCCGG
ACAAAAGATCAGTGAGCAGCTCGGTTGTTCGCGGGCGGCCGTATGGAAACATATCGAGGAGCTGCGCAAAGAAGGGTTTG
AACTCGAAGCGGTGCGCCGCCTCGGCTACCGAATCGTGAGCACGCCGGATAAGGTGACGGCAAACGAAATCCAACTCGGA
TTGAAAACCGAAACGCTTGGCCATCAGATCCATTTTTTTGAGGAAGTCGATTCGACGCAGCGTATCGCGGCGAAGCTGGC
ATATGAGGGAGCGCCGGAAGGGACGCTTGTCATCGCCGAGGAGCAGAAAGCCGGGCGCGGGCGTTTAGACCGGAAATGGT
TTTCCCCACAAGGAACCGGTATTTGGATGAGCCTTATTTTGCGGCCGCCCATTCCCCCGCAGCAGGCGCCGCAGTTGACG
TTGCTTGTTGCCGTGGCGGTCAGTCAAGCGATTCAAGAAGTAACCGGGCTTGTCCCGGACATTAAATGGCCAAACGATAT
TTTGCTTCACGGTAAAAAATGTGTCGGCATTTTAACCGAGCTGCAGGCCGACCCTGACCGCGTCCGTTCGGTCATCATCG
GCATCGGCATTAACGTGAACCAGACGAGTGCGCAATTTCCCGAGGATATCTGCGACATCGCCACATCGCTTGCCATCGAA
AAAGGGGAGCGCATAAAGCGCGCGCCGCTCATCCAAGAAATTTTACTTCAGCTTGAGCGGTTATATAAGCAATATTTGGA
GCATGGCTTCCGTCCAATTAAGCTTCTTTGGGAAGGATACGCGGTTTCGATTGGCAAGCCGGTGACAGCAAGGACATTGG
GCAAGGTGATACGCGGCATCGCGCGCGGCATTACCGATGACGGTCTGCTGATCCTCGAGGATGAACAAAACCAACTTCAT
TATATTCATTCGGCCGATATTCAACTGTAA

Upstream 100 bases:

>100_bases
TCGGGAAACCAGCCGGTCCGTGGGTGAAAGAGACGCTGGATGCCATATGGCGGGCGGTCGTCAACGGTGAAGTTGAAAAC
GAGAAGGAGCGGATTTACGC

Downstream 100 bases:

>100_bases
ATTTCCACTGGAAAAATTGTGACAATTTTGTTAAAATACAAGGCGAGGAAAAGGGCAGTATCTTTGTCGAAAAGAACTGC
ACCGCGCCGGCAAAATGAAT

Product: BirA bifunctional protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAHGAQSDTRKKLLELFAEANGEFLSGQKISEQLGCSRAAVWKHIEELRKEGFELEAVRRLGYRIVSTPDKVTANEIQLG
LKTETLGHQIHFFEEVDSTQRIAAKLAYEGAPEGTLVIAEEQKAGRGRLDRKWFSPQGTGIWMSLILRPPIPPQQAPQLT
LLVAVAVSQAIQEVTGLVPDIKWPNDILLHGKKCVGILTELQADPDRVRSVIIGIGINVNQTSAQFPEDICDIATSLAIE
KGERIKRAPLIQEILLQLERLYKQYLEHGFRPIKLLWEGYAVSIGKPVTARTLGKVIRGIARGITDDGLLILEDEQNQLH
YIHSADIQL

Sequences:

>Translated_329_residues
MAHGAQSDTRKKLLELFAEANGEFLSGQKISEQLGCSRAAVWKHIEELRKEGFELEAVRRLGYRIVSTPDKVTANEIQLG
LKTETLGHQIHFFEEVDSTQRIAAKLAYEGAPEGTLVIAEEQKAGRGRLDRKWFSPQGTGIWMSLILRPPIPPQQAPQLT
LLVAVAVSQAIQEVTGLVPDIKWPNDILLHGKKCVGILTELQADPDRVRSVIIGIGINVNQTSAQFPEDICDIATSLAIE
KGERIKRAPLIQEILLQLERLYKQYLEHGFRPIKLLWEGYAVSIGKPVTARTLGKVIRGIARGITDDGLLILEDEQNQLH
YIHSADIQL
>Mature_328_residues
AHGAQSDTRKKLLELFAEANGEFLSGQKISEQLGCSRAAVWKHIEELRKEGFELEAVRRLGYRIVSTPDKVTANEIQLGL
KTETLGHQIHFFEEVDSTQRIAAKLAYEGAPEGTLVIAEEQKAGRGRLDRKWFSPQGTGIWMSLILRPPIPPQQAPQLTL
LVAVAVSQAIQEVTGLVPDIKWPNDILLHGKKCVGILTELQADPDRVRSVIIGIGINVNQTSAQFPEDICDIATSLAIEK
GERIKRAPLIQEILLQLERLYKQYLEHGFRPIKLLWEGYAVSIGKPVTARTLGKVIRGIARGITDDGLLILEDEQNQLHY
IHSADIQL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=327, Percent_Identity=26.605504587156, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1790408, Length=304, Percent_Identity=32.5657894736842, Blast_Score=138, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36636; Mature: 36505

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHGAQSDTRKKLLELFAEANGEFLSGQKISEQLGCSRAAVWKHIEELRKEGFELEAVRR
CCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
LGYRIVSTPDKVTANEIQLGLKTETLGHQIHFFEEVDSTQRIAAKLAYEGAPEGTLVIAE
CCCEEECCCCCCCHHHHEECEEHHHHCCHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEE
EQKAGRGRLDRKWFSPQGTGIWMSLILRPPIPPQQAPQLTLLVAVAVSQAIQEVTGLVPD
CCCCCCCCCCCCCCCCCCCCCEEHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IKWPNDILLHGKKCVGILTELQADPDRVRSVIIGIGINVNQTSAQFPEDICDIATSLAIE
CCCCCHHEECCHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCHHCHHHHHHHHHHHHHH
KGERIKRAPLIQEILLQLERLYKQYLEHGFRPIKLLWEGYAVSIGKPVTARTLGKVIRGI
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHEECCEEEECCCCHHHHHHHHHHHHH
ARGITDDGLLILEDEQNQLHYIHSADIQL
HCCCCCCCEEEEECCCCCEEEEECCCCCC
>Mature Secondary Structure 
AHGAQSDTRKKLLELFAEANGEFLSGQKISEQLGCSRAAVWKHIEELRKEGFELEAVRR
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
LGYRIVSTPDKVTANEIQLGLKTETLGHQIHFFEEVDSTQRIAAKLAYEGAPEGTLVIAE
CCCEEECCCCCCCHHHHEECEEHHHHCCHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEE
EQKAGRGRLDRKWFSPQGTGIWMSLILRPPIPPQQAPQLTLLVAVAVSQAIQEVTGLVPD
CCCCCCCCCCCCCCCCCCCCCEEHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IKWPNDILLHGKKCVGILTELQADPDRVRSVIIGIGINVNQTSAQFPEDICDIATSLAIE
CCCCCHHEECCHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCHHCHHHHHHHHHHHHHH
KGERIKRAPLIQEILLQLERLYKQYLEHGFRPIKLLWEGYAVSIGKPVTARTLGKVIRGI
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHEECCEEEECCCCHHHHHHHHHHHHH
ARGITDDGLLILEDEQNQLHYIHSADIQL
HCCCCCCCEEEEECCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]