BacMap

Definition	Geobacillus thermodenitrificans NG80-2 chromosome, complete genome.
Accession	NC_009328
Length	3,550,319

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The map label for this gene is paaF [H]

Identifier: 138895571

GI number: 138895571

Start: 2048377

End: 2049153

Strand: Reverse

Name: paaF [H]

Synonym: GTNG_1924

Alternate gene names: 138895571

Gene position: 2049153-2048377 (Counterclockwise)

Preceding gene: 138895572

Following gene: 138895570

Centisome position: 57.72

GC content: 55.86

Gene sequence:

>777_bases
ATGAGTGAGTTCGTTTCGATCGCTGTGCGCCAAGAGGGGCCGGTTGGCGTCATTGAACTCGCCCGTCCGAATGTATTGAA
TGCGCTCAGTCGCCAAATGGTGTCAGAAATTGTTGCGGCGGTCGAATCGTTCGATCGGGATGAAGAAGTGCGCGTCATCG
TGCTCACCGGGCGTGGGCGGGCGTTCGCTGCTGGGGCGGATATTCAAGAGATGGCCAACGATGATCCGGTTCGGCTCGAG
TGGTTGAACCAATTTGCGGATTGGGATCGCCTTTCGCTTGTAAAAACGCCGATGATCGCTGCCGTCAACGGCTTAGCGCT
CGGCGGCGGATTCGAGCTGGCGTTGTCCTGTGATTTGATCATCGCCTCCGCCGCGGCGGAATTCGGTTTTCCAGAGGTTA
ACCTTGGCGTTATGCCAGGGGCGGGAGGTACGCAGCGGTTGACGAAGCTCATCGGACCGAAACAGGCGCTCGCGTGGTTA
TGGACGGGGGAGCGCTTATCGGCGAAAGAGGCGCATGCATTGGGTGTCGTCACCCGCGTTGTGAGTCCGGAACTGTTAAT
GGAAGAGACGATGCGGCTTGCCCGGCGCCTTGCCGAACAGCCGCCGCTAGCCCTTCGCCTTATCAAAGAAGCAGTGCAAA
AAGCAGTAGACTATCCGCTCTATGAAGGCATGCAGTTTGAGCGGAAAAACTTCTACCTTTTGTTTGCTTCAGAAGACCAA
AAAGAAGGAATGGCGGCGTTTCTTGAAAAACGAAAACCGCAATTCCGTGGGAAATAA

Upstream 100 bases:

>100_bases
CAAGAGGCGATGAAAACAAAAGAAGCGAAAGCATCAGCAAAAGATGTCATGTCATTTGCCGGGGATCTTGTCACCTTTAT
GGTTGGTGAGGAAGTGGAAC

Downstream 100 bases:

>100_bases
GGGGGGCGCCTCATGTACGAGACAATTCGATATGAAGTGGATGGCCAAGTTGCTTGGCTGACGCTCAACCGCCCGGAGCA
GCTCAACTCTTTTACGGAAC

Product: enoyl CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSEFVSIAVRQEGPVGVIELARPNVLNALSRQMVSEIVAAVESFDRDEEVRVIVLTGRGRAFAAGADIQEMANDDPVRLE
WLNQFADWDRLSLVKTPMIAAVNGLALGGGFELALSCDLIIASAAAEFGFPEVNLGVMPGAGGTQRLTKLIGPKQALAWL
WTGERLSAKEAHALGVVTRVVSPELLMEETMRLARRLAEQPPLALRLIKEAVQKAVDYPLYEGMQFERKNFYLLFASEDQ
KEGMAAFLEKRKPQFRGK

Sequences:

>Translated_258_residues
MSEFVSIAVRQEGPVGVIELARPNVLNALSRQMVSEIVAAVESFDRDEEVRVIVLTGRGRAFAAGADIQEMANDDPVRLE
WLNQFADWDRLSLVKTPMIAAVNGLALGGGFELALSCDLIIASAAAEFGFPEVNLGVMPGAGGTQRLTKLIGPKQALAWL
WTGERLSAKEAHALGVVTRVVSPELLMEETMRLARRLAEQPPLALRLIKEAVQKAVDYPLYEGMQFERKNFYLLFASEDQ
KEGMAAFLEKRKPQFRGK
>Mature_257_residues
SEFVSIAVRQEGPVGVIELARPNVLNALSRQMVSEIVAAVESFDRDEEVRVIVLTGRGRAFAAGADIQEMANDDPVRLEW
LNQFADWDRLSLVKTPMIAAVNGLALGGGFELALSCDLIIASAAAEFGFPEVNLGVMPGAGGTQRLTKLIGPKQALAWLW
TGERLSAKEAHALGVVTRVVSPELLMEETMRLARRLAEQPPLALRLIKEAVQKAVDYPLYEGMQFERKNFYLLFASEDQK
EGMAAFLEKRKPQFRGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=44.9799196787149, Blast_Score=221, Evalue=4e-58,
Organism=Homo sapiens, GI68989263, Length=286, Percent_Identity=32.1678321678322, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4502327, Length=249, Percent_Identity=32.5301204819277, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=31.25, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=32.1138211382114, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=27.2, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI20127408, Length=241, Percent_Identity=32.7800829875519, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI45643119, Length=223, Percent_Identity=30.9417040358744, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI260275230, Length=223, Percent_Identity=30.9417040358744, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI260274832, Length=223, Percent_Identity=30.9417040358744, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI261878539, Length=182, Percent_Identity=35.1648351648352, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=27.1929824561404, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=27.1929824561404, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI37594469, Length=186, Percent_Identity=27.9569892473118, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI37594471, Length=186, Percent_Identity=27.9569892473118, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI25453481, Length=261, Percent_Identity=25.6704980842912, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI51538573, Length=261, Percent_Identity=25.6704980842912, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4757968, Length=255, Percent_Identity=24.3137254901961, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI49355787, Length=255, Percent_Identity=24.3137254901961, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4757966, Length=250, Percent_Identity=26, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI51538581, Length=250, Percent_Identity=26, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI221136756, Length=266, Percent_Identity=24.812030075188, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI221136753, Length=266, Percent_Identity=24.812030075188, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI62530384, Length=243, Percent_Identity=26.7489711934156, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221307494, Length=266, Percent_Identity=24.812030075188, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157694520, Length=205, Percent_Identity=27.3170731707317, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI295842266, Length=241, Percent_Identity=26.5560165975104, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI116235487, Length=211, Percent_Identity=26.0663507109005, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=41.4728682170543, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=35.9848484848485, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1787660, Length=267, Percent_Identity=37.0786516853933, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=33.7164750957854, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=32.258064516129, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788682, Length=216, Percent_Identity=34.7222222222222, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=35.5555555555556, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=46, Blast_Score=230, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=42.3236514522822, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=31.5789473684211, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=30.5019305019305, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=34.9726775956284, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=272, Percent_Identity=27.2058823529412, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=259, Percent_Identity=27.4131274131274, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=196, Percent_Identity=28.0612244897959, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=233, Percent_Identity=25.7510729613734, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=25.8687258687259, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=222, Percent_Identity=26.5765765765766, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=222, Percent_Identity=26.5765765765766, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=168, Percent_Identity=29.7619047619048, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=201, Percent_Identity=27.8606965174129, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=44.4881889763779, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=44.4881889763779, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=34.6456692913386, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19920382, Length=271, Percent_Identity=30.6273062730627, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=27.0916334661355, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=29.0076335877863, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650670, Length=248, Percent_Identity=27.4193548387097, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24654903, Length=215, Percent_Identity=26.9767441860465, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571730, Length=179, Percent_Identity=27.9329608938547, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28571729, Length=179, Percent_Identity=27.9329608938547, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=36.2068965517241, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=36.2068965517241, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=36.2068965517241, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583165, Length=256, Percent_Identity=21.875, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28413; Mature: 28282

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFVSIAVRQEGPVGVIELARPNVLNALSRQMVSEIVAAVESFDRDEEVRVIVLTGRGR
CCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
AFAAGADIQEMANDDPVRLEWLNQFADWDRLSLVKTPMIAAVNGLALGGGFELALSCDLI
EEECCCCHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECCCEEEEEEHHHH
IASAAAEFGFPEVNLGVMPGAGGTQRLTKLIGPKQALAWLWTGERLSAKEAHALGVVTRV
HHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCHHHHHEEEECCCCCCHHHHHHHHHHHHH
VSPELLMEETMRLARRLAEQPPLALRLIKEAVQKAVDYPLYEGMQFERKNFYLLFASEDQ
HCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCEECCCCEEEEEECCCC
KEGMAAFLEKRKPQFRGK
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEFVSIAVRQEGPVGVIELARPNVLNALSRQMVSEIVAAVESFDRDEEVRVIVLTGRGR
CCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
AFAAGADIQEMANDDPVRLEWLNQFADWDRLSLVKTPMIAAVNGLALGGGFELALSCDLI
EEECCCCHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECCCEEEEEEHHHH
IASAAAEFGFPEVNLGVMPGAGGTQRLTKLIGPKQALAWLWTGERLSAKEAHALGVVTRV
HHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCHHHHHEEEECCCCCCHHHHHHHHHHHHH
VSPELLMEETMRLARRLAEQPPLALRLIKEAVQKAVDYPLYEGMQFERKNFYLLFASEDQ
HCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCEECCCCEEEEEECCCC
KEGMAAFLEKRKPQFRGK
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]