BacMap

Definition	Geobacillus thermodenitrificans NG80-2 chromosome, complete genome.
Accession	NC_009328
Length	3,550,319

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The map label for this gene is paaG [H]

Identifier: 138895570

GI number: 138895570

Start: 2047591

End: 2048364

Strand: Reverse

Name: paaG [H]

Synonym: GTNG_1923

Alternate gene names: 138895570

Gene position: 2048364-2047591 (Counterclockwise)

Preceding gene: 138895571

Following gene: 138895569

Centisome position: 57.7

GC content: 54.39

Gene sequence:

>774_bases
ATGTACGAGACAATTCGATATGAAGTGGATGGCCAAGTTGCTTGGCTGACGCTCAACCGCCCGGAGCAGCTCAACTCTTT
TACGGAACAAATGAACAAGGAAATGACAAAGGCGCTAAAACAGGCCGGAGCAGATCGTGATGTCCGTTGTGTGGTCATCA
CAGGCGCAGGACGGGCGTTTTGTGCTGGGGAAGATTTGAGCGGCGTAACGGAAGAGATGGATCATGGGGAAGTGCTGCGC
ACTCGATACGCACCGATGATGAAAGCGCTCTACAGCCTGGGAAAACCGGTTGTCGCCGCGGTGAATGGGGTAGCGGCCGG
TGCGGGCATGAGCTTAGCGCTCGCGTGTGATTTCCGTCTTCTCTCGGAAAAAGCGAGTTTCGCCCCGGCATTTATCCATG
TGGGACTTGTGCCGGATGCTGGTCACCTGTATTATTTGCCGCGCCTCATTGGGACGGCAAAGGCGCTTGAACTTGCTGTG
CTTGGGGAAAAAGTAGCGGCTACGGAAGCCGTCGCGCTCGGACTAGCGACAACCGTCATTCCGCAAGATCGTTGGGAAGA
GGAAGTGAGGCAGTTTGCCGAGCGATTGGCTGCTATGCCGACGAAGGCGATTGGACTTGTGAAACGGTTGCTTCACGAAA
GTCAAGAAGCGACGTTTGACCGTTACCTCGAACGAGAAGCAGAATGCCAACGCATTGCTGGATTGACGGCAGACCACCGC
GAAGGCGTCAAGGCGTTTTTTGAGAAACGGAAACCAATATTCCAGGGAAACTAA

Upstream 100 bases:

>100_bases
CTTCTACCTTTTGTTTGCTTCAGAAGACCAAAAAGAAGGAATGGCGGCGTTTCTTGAAAAACGAAAACCGCAATTCCGTG
GGAAATAAGGGGGGCGCCTC

Downstream 100 bases:

>100_bases
GCAAGGGGGAAGAAATGATGGCAACTGTCAAACAAAAGAAAATGGAGCGAATGCCGACGAAACGCGATGTTTATCAGCTG
ATTATTAACGGCGAGCGGGT

Product: enoyl CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MYETIRYEVDGQVAWLTLNRPEQLNSFTEQMNKEMTKALKQAGADRDVRCVVITGAGRAFCAGEDLSGVTEEMDHGEVLR
TRYAPMMKALYSLGKPVVAAVNGVAAGAGMSLALACDFRLLSEKASFAPAFIHVGLVPDAGHLYYLPRLIGTAKALELAV
LGEKVAATEAVALGLATTVIPQDRWEEEVRQFAERLAAMPTKAIGLVKRLLHESQEATFDRYLEREAECQRIAGLTADHR
EGVKAFFEKRKPIFQGN

Sequences:

>Translated_257_residues
MYETIRYEVDGQVAWLTLNRPEQLNSFTEQMNKEMTKALKQAGADRDVRCVVITGAGRAFCAGEDLSGVTEEMDHGEVLR
TRYAPMMKALYSLGKPVVAAVNGVAAGAGMSLALACDFRLLSEKASFAPAFIHVGLVPDAGHLYYLPRLIGTAKALELAV
LGEKVAATEAVALGLATTVIPQDRWEEEVRQFAERLAAMPTKAIGLVKRLLHESQEATFDRYLEREAECQRIAGLTADHR
EGVKAFFEKRKPIFQGN
>Mature_257_residues
MYETIRYEVDGQVAWLTLNRPEQLNSFTEQMNKEMTKALKQAGADRDVRCVVITGAGRAFCAGEDLSGVTEEMDHGEVLR
TRYAPMMKALYSLGKPVVAAVNGVAAGAGMSLALACDFRLLSEKASFAPAFIHVGLVPDAGHLYYLPRLIGTAKALELAV
LGEKVAATEAVALGLATTVIPQDRWEEEVRQFAERLAAMPTKAIGLVKRLLHESQEATFDRYLEREAECQRIAGLTADHR
EGVKAFFEKRKPIFQGN

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=32.1138211382114, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=30.5882352941176, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=29.7297297297297, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=29.7297297297297, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=29.7297297297297, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI70995211, Length=194, Percent_Identity=34.5360824742268, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=31.9371727748691, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=32.4607329842932, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI37594469, Length=191, Percent_Identity=32.4607329842932, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757968, Length=247, Percent_Identity=27.9352226720648, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI49355787, Length=247, Percent_Identity=27.9352226720648, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI25453481, Length=247, Percent_Identity=27.5303643724696, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI51538573, Length=247, Percent_Identity=27.5303643724696, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI4757966, Length=256, Percent_Identity=28.515625, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI51538581, Length=256, Percent_Identity=28.515625, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI116235487, Length=170, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136756, Length=224, Percent_Identity=28.125, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221136753, Length=224, Percent_Identity=28.125, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221307494, Length=224, Percent_Identity=28.125, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI20127408, Length=239, Percent_Identity=26.7782426778243, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=29.8387096774194, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157694516, Length=224, Percent_Identity=23.6607142857143, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI213417737, Length=224, Percent_Identity=23.6607142857143, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=42.1455938697318, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=34.6938775510204, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1788597, Length=267, Percent_Identity=32.2097378277154, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=29.7560975609756, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=23.0188679245283, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=24.7252747252747, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=33.195020746888, Blast_Score=136, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=33.976833976834, Blast_Score=134, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=32.258064516129, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17535521, Length=210, Percent_Identity=27.6190476190476, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=27.3092369477912, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=29.0697674418605, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=28, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=224, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=187, Percent_Identity=30.4812834224599, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=187, Percent_Identity=30.4812834224599, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=26.8774703557312, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=27.807486631016, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=186, Percent_Identity=29.5698924731183, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=186, Percent_Identity=29.5698924731183, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=186, Percent_Identity=29.5698924731183, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=27.979274611399, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=33.203125, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=33.203125, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24654903, Length=243, Percent_Identity=29.6296296296296, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=31.3492063492063, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19922422, Length=241, Percent_Identity=26.5560165975104, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357171, Length=261, Percent_Identity=27.9693486590038, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=28.6821705426357, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24650670, Length=249, Percent_Identity=26.5060240963855, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571730, Length=167, Percent_Identity=32.3353293413174, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571729, Length=167, Percent_Identity=32.3353293413174, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28217; Mature: 28217

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYETIRYEVDGQVAWLTLNRPEQLNSFTEQMNKEMTKALKQAGADRDVRCVVITGAGRAF
CCCEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEE
CAGEDLSGVTEEMDHGEVLRTRYAPMMKALYSLGKPVVAAVNGVAAGAGMSLALACDFRL
ECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHH
LSEKASFAPAFIHVGLVPDAGHLYYLPRLIGTAKALELAVLGEKVAATEAVALGLATTVI
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PQDRWEEEVRQFAERLAAMPTKAIGLVKRLLHESQEATFDRYLEREAECQRIAGLTADHR
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
EGVKAFFEKRKPIFQGN
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYETIRYEVDGQVAWLTLNRPEQLNSFTEQMNKEMTKALKQAGADRDVRCVVITGAGRAF
CCCEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEE
CAGEDLSGVTEEMDHGEVLRTRYAPMMKALYSLGKPVVAAVNGVAAGAGMSLALACDFRL
ECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHH
LSEKASFAPAFIHVGLVPDAGHLYYLPRLIGTAKALELAVLGEKVAATEAVALGLATTVI
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PQDRWEEEVRQFAERLAAMPTKAIGLVKRLLHESQEATFDRYLEREAECQRIAGLTADHR
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
EGVKAFFEKRKPIFQGN
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]