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The map label for this gene is yiaO [H]

Identifier: 138895522

GI number: 138895522

Start: 1996040

End: 1997086

Strand: Reverse

Name: yiaO [H]

Synonym: GTNG_1870

Alternate gene names: 138895522

Gene position: 1997086-1996040 (Counterclockwise)

Preceding gene: 138895523

Following gene: 138895521

Centisome position: 56.25

GC content: 42.6

Gene sequence:

>1047_bases
ATGAAAAAGAGGAATGTACTTAGAAAGATGATGGCTGGCTGTATGGCCATGGGATTGCTTTTCCTGACTGCCTGCAGCTC
AGGAACGACCCAAGGGAGCGGTACAGACAAGGGGATCCAACAACGGGTAATAAAGGCGGGCATCGGTCTAAACGAAGACC
ATCCCGAAGGCCAAGGATTGCTTAAGTTCAAAGAGATTGTTGAGCAGAAAAGTGGCGGAAAATTAAAAGTTCAAACATAC
TTCAGCGCTACTTTGGGCGATGACTTAAAAATGACGGAAGCGCTTCAAGCCGGCACACAAGAAATTACGATCCCGTCTAC
TTCGCCATTGGTTGGAATTATGAAGGAATTTGGTATTTTTGATTTTCCGTTTCTCTTTAACACGTCCGAAGAAGCGGATG
CCATTTTGGACGGACCGATAGGCCAAAAACTATTGGAAAAAATGCCTGAATATGGATTGATAGGGCTTGGCTATTGGGAA
AACGGATTCCGTCATGTAACGAATAGCAAACGTGCTATCGAGACAGCGGATGACTTTAAAGGATTGAAATTACGGACAAT
GCAAAACGAAGTGCATATTGATGCGTTTAAAGAACTTGGTGCCAATCCGACGCCAATGCCTTTCTCGGAAGTGTTCACTG
CATTGGAAAGCCATACGATTGATGGTCAAGAAAATCCGTTGGCAACGATTAAATCGAACAAGTTTTACGAAGTGCAAAAA
CATTTAAGCTTAACCAAACACGTTTATACTCCGTTTGTGTTCCTTGTCAGCAAGAAGTTTTGGGATACGCTGTCGTCTGA
GGAACAAAAGATTTTGCAGGAAGCTGCCATCGAAGCAGGGAAATATCAACGGCAGCTAAGCCGGGAAGAGGATCAAAAAG
CATTGGATGAGTTGAAAAAGACAGGCGTAAAAATTAATGAAATTAGCGATCAAGAACGTCAAAAAATGGCGGAAATCATT
CAACCGGTTGTTGAAACATATGCAAAAAAATTCGGAGACGATCTTGTAAACGAACTATACAACGAGTTGGATAAAATTCG
CCAGTAA

Upstream 100 bases:

>100_bases
CTTCTGTTGCTCATTTTCTTCCCATCGCTCGTCACTGTGCCACTTGAGTGGTTTGCGGGCAATTGATAAATGCCATTTAT
AACTAGGGGGATTGAAACCG

Downstream 100 bases:

>100_bases
GCAGTAGTGAAGTTGGTGGTTGTTAGGTCGAGGGGGCAATGAGCTGTATTTGCCCTCTCCCTTTCTAAAATGTGCTAGGG
ATCGAGCGAACAATTCAAAG

Product: ABC transporter periplasmic solute-binding protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKRNVLRKMMAGCMAMGLLFLTACSSGTTQGSGTDKGIQQRVIKAGIGLNEDHPEGQGLLKFKEIVEQKSGGKLKVQTY
FSATLGDDLKMTEALQAGTQEITIPSTSPLVGIMKEFGIFDFPFLFNTSEEADAILDGPIGQKLLEKMPEYGLIGLGYWE
NGFRHVTNSKRAIETADDFKGLKLRTMQNEVHIDAFKELGANPTPMPFSEVFTALESHTIDGQENPLATIKSNKFYEVQK
HLSLTKHVYTPFVFLVSKKFWDTLSSEEQKILQEAAIEAGKYQRQLSREEDQKALDELKKTGVKINEISDQERQKMAEII
QPVVETYAKKFGDDLVNELYNELDKIRQ

Sequences:

>Translated_348_residues
MKKRNVLRKMMAGCMAMGLLFLTACSSGTTQGSGTDKGIQQRVIKAGIGLNEDHPEGQGLLKFKEIVEQKSGGKLKVQTY
FSATLGDDLKMTEALQAGTQEITIPSTSPLVGIMKEFGIFDFPFLFNTSEEADAILDGPIGQKLLEKMPEYGLIGLGYWE
NGFRHVTNSKRAIETADDFKGLKLRTMQNEVHIDAFKELGANPTPMPFSEVFTALESHTIDGQENPLATIKSNKFYEVQK
HLSLTKHVYTPFVFLVSKKFWDTLSSEEQKILQEAAIEAGKYQRQLSREEDQKALDELKKTGVKINEISDQERQKMAEII
QPVVETYAKKFGDDLVNELYNELDKIRQ
>Mature_348_residues
MKKRNVLRKMMAGCMAMGLLFLTACSSGTTQGSGTDKGIQQRVIKAGIGLNEDHPEGQGLLKFKEIVEQKSGGKLKVQTY
FSATLGDDLKMTEALQAGTQEITIPSTSPLVGIMKEFGIFDFPFLFNTSEEADAILDGPIGQKLLEKMPEYGLIGLGYWE
NGFRHVTNSKRAIETADDFKGLKLRTMQNEVHIDAFKELGANPTPMPFSEVFTALESHTIDGQENPLATIKSNKFYEVQK
HLSLTKHVYTPFVFLVSKKFWDTLSSEEQKILQEAAIEAGKYQRQLSREEDQKALDELKKTGVKINEISDQERQKMAEII
QPVVETYAKKFGDDLVNELYNELDKIRQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=259, Percent_Identity=40.9266409266409, Blast_Score=218, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 39072; Mature: 39072

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRNVLRKMMAGCMAMGLLFLTACSSGTTQGSGTDKGIQQRVIKAGIGLNEDHPEGQGL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCH
LKFKEIVEQKSGGKLKVQTYFSATLGDDLKMTEALQAGTQEITIPSTSPLVGIMKEFGIF
HHHHHHHHHCCCCEEEEEEEEHHHCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCC
DFPFLFNTSEEADAILDGPIGQKLLEKMPEYGLIGLGYWENGFRHVTNSKRAIETADDFK
CCCHHCCCCCHHHHHHCCHHHHHHHHHCCCCCEEEECHHHCCHHHHCCCCHHHCCHHHCC
GLKLRTMQNEVHIDAFKELGANPTPMPFSEVFTALESHTIDGQENPLATIKSNKFYEVQK
CCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHH
HLSLTKHVYTPFVFLVSKKFWDTLSSEEQKILQEAAIEAGKYQRQLSREEDQKALDELKK
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVKINEISDQERQKMAEIIQPVVETYAKKFGDDLVNELYNELDKIRQ
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKRNVLRKMMAGCMAMGLLFLTACSSGTTQGSGTDKGIQQRVIKAGIGLNEDHPEGQGL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCH
LKFKEIVEQKSGGKLKVQTYFSATLGDDLKMTEALQAGTQEITIPSTSPLVGIMKEFGIF
HHHHHHHHHCCCCEEEEEEEEHHHCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCC
DFPFLFNTSEEADAILDGPIGQKLLEKMPEYGLIGLGYWENGFRHVTNSKRAIETADDFK
CCCHHCCCCCHHHHHHCCHHHHHHHHHCCCCCEEEECHHHCCHHHHCCCCHHHCCHHHCC
GLKLRTMQNEVHIDAFKELGANPTPMPFSEVFTALESHTIDGQENPLATIKSNKFYEVQK
CCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHH
HLSLTKHVYTPFVFLVSKKFWDTLSSEEQKILQEAAIEAGKYQRQLSREEDQKALDELKK
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVKINEISDQERQKMAEIIQPVVETYAKKFGDDLVNELYNELDKIRQ
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]