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The map label for this gene is mtlF [H]

Identifier: 138895497

GI number: 138895497

Start: 1966878

End: 1967321

Strand: Reverse

Name: mtlF [H]

Synonym: GTNG_1845

Alternate gene names: 138895497

Gene position: 1967321-1966878 (Counterclockwise)

Preceding gene: 138895498

Following gene: 138895496

Centisome position: 55.41

GC content: 49.32

Gene sequence:

>444_bases
ATGTTGGCTTATTCAATTTTAAAGAAAGAAAACATTGTGCTAAATGCTCAACCAAAAACGAAAGAAGAAGCCATTCGCCT
AGCTGGCGAAGTGCTCGTCAAGCAAGGGTATGTGGATCCATCGTATATTGATGCAATGCTTGAGCGCGAACAGTTGACGA
CTACGTATATCGGCAACAATGTCGCTATCCCGCACGGAACGGAAGAAGCCAAATCGCTCGTTAAGCAGTCCGGCATTTCG
ATCGTGCAAGTTCCAGGAGGCGTCGACTTTGGCGGGGGCAACCAAGCAACGATTGTCATCGGCATCGCCGGAAAAGACGG
AGAACATTTAGACATTCTTTCGAAACTCGCGCTCGTCTGCGCGGAAGCGGACAATGTTGCGGCTATGGCGAACGCCAAAA
CAGCGGAGGAAATTCTTACGCTGCTTAATGAGGTGAACGGCTGA

Upstream 100 bases:

>100_bases
CGTTGGCTCGTGGCGGCGAAAACGAGGTATATTCGCTCGTTAGCAGCCATTTAGAACAATTTTTCGATCATTATTGGACA
ACAACGAAGGAGTGAAGGTC

Downstream 100 bases:

>100_bases
TGAGGGCCGTTCACTTCGGCGCAGGCAATATTGGCCGCGGTTTTATCGGCAGCCTGCTTGCAGCGTCGGGATATGATGTC
GTGTTTGTCGATGTCAACGA

Product: mannitol enzyme IIA (PTS system)

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLAYSILKKENIVLNAQPKTKEEAIRLAGEVLVKQGYVDPSYIDAMLEREQLTTTYIGNNVAIPHGTEEAKSLVKQSGIS
IVQVPGGVDFGGGNQATIVIGIAGKDGEHLDILSKLALVCAEADNVAAMANAKTAEEILTLLNEVNG

Sequences:

>Translated_147_residues
MLAYSILKKENIVLNAQPKTKEEAIRLAGEVLVKQGYVDPSYIDAMLEREQLTTTYIGNNVAIPHGTEEAKSLVKQSGIS
IVQVPGGVDFGGGNQATIVIGIAGKDGEHLDILSKLALVCAEADNVAAMANAKTAEEILTLLNEVNG
>Mature_147_residues
MLAYSILKKENIVLNAQPKTKEEAIRLAGEVLVKQGYVDPSYIDAMLEREQLTTTYIGNNVAIPHGTEEAKSLVKQSGIS
IVQVPGGVDFGGGNQATIVIGIAGKDGEHLDILSKLALVCAEADNVAAMANAKTAEEILTLLNEVNG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=138, Percent_Identity=45.6521739130435, Blast_Score=123, Evalue=5e-30,
Organism=Escherichia coli, GI1788494, Length=122, Percent_Identity=42.6229508196721, Blast_Score=102, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15532; Mature: 15532

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAYSILKKENIVLNAQPKTKEEAIRLAGEVLVKQGYVDPSYIDAMLEREQLTTTYIGNN
CCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCE
VAIPHGTEEAKSLVKQSGISIVQVPGGVDFGGGNQATIVIGIAGKDGEHLDILSKLALVC
EECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
AEADNVAAMANAKTAEEILTLLNEVNG
HCCCCEEEHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLAYSILKKENIVLNAQPKTKEEAIRLAGEVLVKQGYVDPSYIDAMLEREQLTTTYIGNN
CCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCE
VAIPHGTEEAKSLVKQSGISIVQVPGGVDFGGGNQATIVIGIAGKDGEHLDILSKLALVC
EECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
AEADNVAAMANAKTAEEILTLLNEVNG
HCCCCEEEHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8824601 [H]