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The map label for this gene is citS [H]

Identifier: 138895492

GI number: 138895492

Start: 1960576

End: 1962174

Strand: Reverse

Name: citS [H]

Synonym: GTNG_1840

Alternate gene names: 138895492

Gene position: 1962174-1960576 (Counterclockwise)

Preceding gene: 138895493

Following gene: 138895491

Centisome position: 55.27

GC content: 47.72

Gene sequence:

>1599_bases
ATGAAGCTTCAGACAAGGTTAATGGTCATTATTTGTTCCCTGCTATTATTTGTGATCATTTTTCTGACTTTTTTGTTTCA
ACATATGTTTGCTGCTACACTGAAAGAGCAAATCGGAATGCGGGCGCTGAATGTAGCGGAGACGGTCGCTTCGACTCCGC
TTGTGCGCGAGGCATTTCGTGACTCAAATCCGTCCGTGCGTTTGCAGCCGTTTGCAGAGCGCATTCGCCAAAAGACAGGG
GCGGAATACGTAGTGATCGGCAACCGACAAGGCATTCGTTATGCCCATCCGCTGACGGAACGAATTGGGAAGTCAATGAT
TGGGGGAGATAACAAGGAGGTACTGAAAGGCAAATCCATTATTTCTGAGGCGGTCGGTTCACTCGGTCCAGCCATCCGCG
GGAAGGCGCCGATTTTTGATGAGAACGGAAGCGTGATCGGCATTGTTTCGGTCGGGTTTTTGTTAGAAGATATTCAGCGC
ACCGTGTGGTCGTATAGCATGAAGATTTTCTTTTTTTCCGTTCTTGCACTTTTGTTTGGGGCAGTTGGTGCGGTGGCGAT
TGCCAAGGCAGTGAAAAAATCGATTCACGGCCTTGAACCGGAAGAAATCGGCTTGTTGTACCAAGAAAAACAGGGAATTT
TAGAGGCGATTCGTGAAGGAATCGTCGCCGTCAATCAAGAAGGGACAATTACGATGGTCAATCAAACTGCTTTGAAGTTG
CTTGGCTATGACAATGAGCGTAACGTATTAGGAACGCCTATCTTGCAACTGATTCCCCATTCCCGGCTGCCTGAAGTAAT
TCGAACGGGACAAGCGGAATATGATGATGAGATGGTTTTAGGAGGAGAGACCGTCATTGCGAACAGGATTCCGATCAAAA
ACAAGCAAGGGCGGGTGATCGGAGCGGTATCGACGTTTCGCAACAAGTCGGAGTTGTACCGCCTGACGAAAGAGCTGTCA
CAGCTGCGAACCTATGCCGATGCATTGCGCGCACAAACGCATGAGTTTTCCAACAAACTGTATTTAATCTCAGGCCTGAT
TCAGCTTGAATCATACGAAGAGGCGCTAGAACTCATCACAAAGGAAACAGACTTGCAGCAAAACATCGTTCGATTTGTCA
TGAAAGAAATCCCTGACCCTATCATTGGTGGACTGCTGCTTGGCAAATTCAACCGTGCCAATGAGTTAAAAATAGCATTT
GAAATTGATCGGGAAAGCAGTTTTCGAGATGTACCTCCATGGATGGATCGCGATCATTTGGTGACGATTATCGGCAACTT
GTTGGATAACGCGATGGAAGCGGTTCTTCACAACGGAAAAGAAGAGAAGCGAGTGACCGTTTTTTTGACCGACCTCGGCA
ATGACTTGATTATTGAAGTCGAAGATAACGGATTAGGCATCGATCCGGCTGTGGCAGAACGAATATACGACCGCGGTTTT
TCGACCAAAGCCAGCGGACTCCGTGGGTATGGACTTGATCTTGTCAAGAAGGCGCTGGAAATGCTTGACGGGCAAATCAC
TTATCAATCGAAACAAGGGGAGGGAACGGTGTTTACCGTCATTATTCCGAAGCAGCTGGCCGACGCCAATCACCATTGA

Upstream 100 bases:

>100_bases
CGATCATCGTCAACGAAGATATGTAATAATTAAGGAAGAAAGACGTTTCGCCTAACACGCGGACGGTCTTTCTTTATTTT
CTATTTTGAGAGGAAGGACT

Downstream 100 bases:

>100_bases
GAGGGGGATAGAGAGTCGATGATTCGCGTGCTGATTATCGAAGACGACCATCGTATTGCCGAGATTAACCGACGCTTTGT
GGAAAAAGTAGAAGGGTATG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKLQTRLMVIICSLLLFVIIFLTFLFQHMFAATLKEQIGMRALNVAETVASTPLVREAFRDSNPSVRLQPFAERIRQKTG
AEYVVIGNRQGIRYAHPLTERIGKSMIGGDNKEVLKGKSIISEAVGSLGPAIRGKAPIFDENGSVIGIVSVGFLLEDIQR
TVWSYSMKIFFFSVLALLFGAVGAVAIAKAVKKSIHGLEPEEIGLLYQEKQGILEAIREGIVAVNQEGTITMVNQTALKL
LGYDNERNVLGTPILQLIPHSRLPEVIRTGQAEYDDEMVLGGETVIANRIPIKNKQGRVIGAVSTFRNKSELYRLTKELS
QLRTYADALRAQTHEFSNKLYLISGLIQLESYEEALELITKETDLQQNIVRFVMKEIPDPIIGGLLLGKFNRANELKIAF
EIDRESSFRDVPPWMDRDHLVTIIGNLLDNAMEAVLHNGKEEKRVTVFLTDLGNDLIIEVEDNGLGIDPAVAERIYDRGF
STKASGLRGYGLDLVKKALEMLDGQITYQSKQGEGTVFTVIIPKQLADANHH

Sequences:

>Translated_532_residues
MKLQTRLMVIICSLLLFVIIFLTFLFQHMFAATLKEQIGMRALNVAETVASTPLVREAFRDSNPSVRLQPFAERIRQKTG
AEYVVIGNRQGIRYAHPLTERIGKSMIGGDNKEVLKGKSIISEAVGSLGPAIRGKAPIFDENGSVIGIVSVGFLLEDIQR
TVWSYSMKIFFFSVLALLFGAVGAVAIAKAVKKSIHGLEPEEIGLLYQEKQGILEAIREGIVAVNQEGTITMVNQTALKL
LGYDNERNVLGTPILQLIPHSRLPEVIRTGQAEYDDEMVLGGETVIANRIPIKNKQGRVIGAVSTFRNKSELYRLTKELS
QLRTYADALRAQTHEFSNKLYLISGLIQLESYEEALELITKETDLQQNIVRFVMKEIPDPIIGGLLLGKFNRANELKIAF
EIDRESSFRDVPPWMDRDHLVTIIGNLLDNAMEAVLHNGKEEKRVTVFLTDLGNDLIIEVEDNGLGIDPAVAERIYDRGF
STKASGLRGYGLDLVKKALEMLDGQITYQSKQGEGTVFTVIIPKQLADANHH
>Mature_532_residues
MKLQTRLMVIICSLLLFVIIFLTFLFQHMFAATLKEQIGMRALNVAETVASTPLVREAFRDSNPSVRLQPFAERIRQKTG
AEYVVIGNRQGIRYAHPLTERIGKSMIGGDNKEVLKGKSIISEAVGSLGPAIRGKAPIFDENGSVIGIVSVGFLLEDIQR
TVWSYSMKIFFFSVLALLFGAVGAVAIAKAVKKSIHGLEPEEIGLLYQEKQGILEAIREGIVAVNQEGTITMVNQTALKL
LGYDNERNVLGTPILQLIPHSRLPEVIRTGQAEYDDEMVLGGETVIANRIPIKNKQGRVIGAVSTFRNKSELYRLTKELS
QLRTYADALRAQTHEFSNKLYLISGLIQLESYEEALELITKETDLQQNIVRFVMKEIPDPIIGGLLLGKFNRANELKIAF
EIDRESSFRDVPPWMDRDHLVTIIGNLLDNAMEAVLHNGKEEKRVTVFLTDLGNDLIIEVEDNGLGIDPAVAERIYDRGF
STKASGLRGYGLDLVKKALEMLDGQITYQSKQGEGTVFTVIIPKQLADANHH

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=529, Percent_Identity=34.0264650283554, Blast_Score=301, Evalue=1e-82,
Organism=Escherichia coli, GI1786837, Length=525, Percent_Identity=33.1428571428571, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1788549, Length=512, Percent_Identity=24.21875, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790300, Length=343, Percent_Identity=25.3644314868805, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59160; Mature: 59160

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQTRLMVIICSLLLFVIIFLTFLFQHMFAATLKEQIGMRALNVAETVASTPLVREAFR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHH
DSNPSVRLQPFAERIRQKTGAEYVVIGNRQGIRYAHPLTERIGKSMIGGDNKEVLKGKSI
CCCCCEEECHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
ISEAVGSLGPAIRGKAPIFDENGSVIGIVSVGFLLEDIQRTVWSYSMKIFFFSVLALLFG
HHHHHHHCCCCCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGAVAIAKAVKKSIHGLEPEEIGLLYQEKQGILEAIREGIVAVNQEGTITMVNQTALKL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEHHHHHHH
LGYDNERNVLGTPILQLIPHSRLPEVIRTGQAEYDDEMVLGGETVIANRIPIKNKQGRVI
HCCCCCCCCCCCHHHHHCCCCCCHHHHHCCCCCCCCCEEECCCEEEECCCCCCCCCCCEE
GAVSTFRNKSELYRLTKELSQLRTYADALRAQTHEFSNKLYLISGLIQLESYEEALELIT
EHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
KETDLQQNIVRFVMKEIPDPIIGGLLLGKFNRANELKIAFEIDRESSFRDVPPWMDRDHL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHH
VTIIGNLLDNAMEAVLHNGKEEKRVTVFLTDLGNDLIIEVEDNGLGIDPAVAERIYDRGF
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCC
STKASGLRGYGLDLVKKALEMLDGQITYQSKQGEGTVFTVIIPKQLADANHH
CCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECHHHCCCCCC
>Mature Secondary Structure
MKLQTRLMVIICSLLLFVIIFLTFLFQHMFAATLKEQIGMRALNVAETVASTPLVREAFR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHH
DSNPSVRLQPFAERIRQKTGAEYVVIGNRQGIRYAHPLTERIGKSMIGGDNKEVLKGKSI
CCCCCEEECHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
ISEAVGSLGPAIRGKAPIFDENGSVIGIVSVGFLLEDIQRTVWSYSMKIFFFSVLALLFG
HHHHHHHCCCCCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGAVAIAKAVKKSIHGLEPEEIGLLYQEKQGILEAIREGIVAVNQEGTITMVNQTALKL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEHHHHHHH
LGYDNERNVLGTPILQLIPHSRLPEVIRTGQAEYDDEMVLGGETVIANRIPIKNKQGRVI
HCCCCCCCCCCCHHHHHCCCCCCHHHHHCCCCCCCCCEEECCCEEEECCCCCCCCCCCEE
GAVSTFRNKSELYRLTKELSQLRTYADALRAQTHEFSNKLYLISGLIQLESYEEALELIT
EHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
KETDLQQNIVRFVMKEIPDPIIGGLLLGKFNRANELKIAFEIDRESSFRDVPPWMDRDHL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHH
VTIIGNLLDNAMEAVLHNGKEEKRVTVFLTDLGNDLIIEVEDNGLGIDPAVAERIYDRGF
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCC
STKASGLRGYGLDLVKKALEMLDGQITYQSKQGEGTVFTVIIPKQLADANHH
CCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]