BacMap

Definition	Geobacillus thermodenitrificans NG80-2 chromosome, complete genome.
Accession	NC_009328
Length	3,550,319

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The map label for this gene is paaG [C]

Identifier: 138895191

GI number: 138895191

Start: 1611257

End: 1612036

Strand: Direct

Name: paaG [C]

Synonym: GTNG_1529

Alternate gene names: 138895191

Gene position: 1611257-1612036 (Clockwise)

Preceding gene: 138895190

Following gene: 138895195

Centisome position: 45.38

GC content: 56.03

Gene sequence:

>780_bases
ATGGAAACCATGCATTTGCTTGTGGAACAGTCCGGCTCGGTCTTATGGCTGACATTGAACCGTCCGGAGCGGCTCAATGC
CTTCAGTCCGGAGATGATCGCTGGACTGACTCGTGCAATCGAACGCGCTGCGATGGACGACGCTGTTCGGGCGATCGTTC
TATCCGGTGCCGGCCGGGCATTCAGCGCCGGCGGTGACGTCAAAGCAATGGGGCAGTCGAGCGCCTCGCAGGCGTATGAG
CATATCGGCCGCCTTAATCGGCTCATTATGACGATGAAATCATGTGAAAAACCAATCATTGCTGCTGTGCATGGCGTGGC
GGCTGGGGCAGGGTTCAATTTGGCACTGGCTTGCGATCTCATTTTGGCCGCCGAAGATAGTCAATTCATTTTGAGCTTTG
CGAATGTCGGCCTTGTCTCCGACGGCGGTGGTTCGTATTGGTTGCCGCGGCTTGTCGGCCCTCATTTGGCCAAGCAATTT
TTCTTCACCGCCGAGCCGATTTCGGCCCACCGTCTGTATGAACTCGGTGTGTTAAGCAAGATCGTTCCGGCCGAAGAGTT
GCGCGAGGCGGTGGAAGCGCTCGCTGCCAAACTGGCCGCTAGACCGACAAAAGCGATCGGCAAACAAAAACGGCTGATCG
AGCACGCTTTGACGGCGACGCTCGAAGAAATTCTCGAAGAAGAGCGGCTCATCCAAGGATTGATGGTCGAAACGGACGAC
CATCGCGAAGGGGTGGCGGCGTTTAAAGAGAAGCGAAAGCCGGTGTTTCAGGGAAAATAA

Upstream 100 bases:

>100_bases
GCATCGTACATGACCGGCTCCGATGTGTTGGTCGATGGCGGTTGGCTCATTAAATAGCAATCATGTTCATTGGAAATGAA
AGAAGGAAAGGAAGAGGGAC

Downstream 100 bases:

>100_bases
TCAGCAGTGAGAAACATGGTAACCGGTCTTATTCATATGGGATCTCGACATCTTGATGCCTAAGTGTGAAGCTTCTTGAA
ATGACATCAGCCCCTCCGTC

Product: enoyl CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
METMHLLVEQSGSVLWLTLNRPERLNAFSPEMIAGLTRAIERAAMDDAVRAIVLSGAGRAFSAGGDVKAMGQSSASQAYE
HIGRLNRLIMTMKSCEKPIIAAVHGVAAGAGFNLALACDLILAAEDSQFILSFANVGLVSDGGGSYWLPRLVGPHLAKQF
FFTAEPISAHRLYELGVLSKIVPAEELREAVEALAAKLAARPTKAIGKQKRLIEHALTATLEEILEEERLIQGLMVETDD
HREGVAAFKEKRKPVFQGK

Sequences:

>Translated_259_residues
METMHLLVEQSGSVLWLTLNRPERLNAFSPEMIAGLTRAIERAAMDDAVRAIVLSGAGRAFSAGGDVKAMGQSSASQAYE
HIGRLNRLIMTMKSCEKPIIAAVHGVAAGAGFNLALACDLILAAEDSQFILSFANVGLVSDGGGSYWLPRLVGPHLAKQF
FFTAEPISAHRLYELGVLSKIVPAEELREAVEALAAKLAARPTKAIGKQKRLIEHALTATLEEILEEERLIQGLMVETDD
HREGVAAFKEKRKPVFQGK
>Mature_259_residues
METMHLLVEQSGSVLWLTLNRPERLNAFSPEMIAGLTRAIERAAMDDAVRAIVLSGAGRAFSAGGDVKAMGQSSASQAYE
HIGRLNRLIMTMKSCEKPIIAAVHGVAAGAGFNLALACDLILAAEDSQFILSFANVGLVSDGGGSYWLPRLVGPHLAKQF
FFTAEPISAHRLYELGVLSKIVPAEELREAVEALAAKLAARPTKAIGKQKRLIEHALTATLEEILEEERLIQGLMVETDD
HREGVAAFKEKRKPVFQGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=35.632183908046, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=32.1138211382114, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=29.6442687747036, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI37594469, Length=197, Percent_Identity=31.9796954314721, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI37594471, Length=197, Percent_Identity=31.9796954314721, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4502327, Length=265, Percent_Identity=29.811320754717, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI68989263, Length=182, Percent_Identity=30.2197802197802, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI116235487, Length=210, Percent_Identity=26.1904761904762, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI260274832, Length=252, Percent_Identity=24.2063492063492, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=252, Percent_Identity=24.2063492063492, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=252, Percent_Identity=24.2063492063492, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI25453481, Length=212, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI51538573, Length=212, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4757966, Length=212, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI51538581, Length=212, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI20127408, Length=235, Percent_Identity=25.1063829787234, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4757968, Length=212, Percent_Identity=24.5283018867925, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI49355787, Length=212, Percent_Identity=24.5283018867925, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI221136756, Length=224, Percent_Identity=22.7678571428571, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=224, Percent_Identity=22.7678571428571, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI221307494, Length=207, Percent_Identity=23.1884057971014, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=40, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=32.9411764705882, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=30.4, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=31.6981132075472, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=30.8270676691729, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=29.0816326530612, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790281, Length=196, Percent_Identity=25, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=32.6446280991736, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17536985, Length=235, Percent_Identity=30.2127659574468, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=180, Percent_Identity=31.6666666666667, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=25.6198347107438, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=254, Percent_Identity=27.9527559055118, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=263, Percent_Identity=25.0950570342205, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=189, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=26.1111111111111, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=248, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=194, Percent_Identity=29.8969072164948, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=29.8969072164948, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=182, Percent_Identity=25.8241758241758, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323314, Length=208, Percent_Identity=24.5192307692308, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324754, Length=169, Percent_Identity=23.6686390532544, Blast_Score=62, Evalue=6e-11,
Organism=Drosophila melanogaster, GI20129971, Length=240, Percent_Identity=35, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24653477, Length=240, Percent_Identity=35, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=33.0645161290323, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24654903, Length=219, Percent_Identity=27.8538812785388, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=27.5590551181102, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=30.6201550387597, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=25.4826254826255, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571730, Length=165, Percent_Identity=32.1212121212121, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571729, Length=165, Percent_Identity=32.1212121212121, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24650670, Length=258, Percent_Identity=26.7441860465116, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28000; Mature: 28000

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METMHLLVEQSGSVLWLTLNRPERLNAFSPEMIAGLTRAIERAAMDDAVRAIVLSGAGRA
CCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FSAGGDVKAMGQSSASQAYEHIGRLNRLIMTMKSCEKPIIAAVHGVAAGAGFNLALACDL
ECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHH
ILAAEDSQFILSFANVGLVSDGGGSYWLPRLVGPHLAKQFFFTAEPISAHRLYELGVLSK
HHCCCCCHHHHHHHCCCEEECCCCCEEHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHH
IVPAEELREAVEALAAKLAARPTKAIGKQKRLIEHALTATLEEILEEERLIQGLMVETDD
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HREGVAAFKEKRKPVFQGK
CHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
METMHLLVEQSGSVLWLTLNRPERLNAFSPEMIAGLTRAIERAAMDDAVRAIVLSGAGRA
CCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FSAGGDVKAMGQSSASQAYEHIGRLNRLIMTMKSCEKPIIAAVHGVAAGAGFNLALACDL
ECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHH
ILAAEDSQFILSFANVGLVSDGGGSYWLPRLVGPHLAKQFFFTAEPISAHRLYELGVLSK
HHCCCCCHHHHHHHCCCEEECCCCCEEHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHH
IVPAEELREAVEALAAKLAARPTKAIGKQKRLIEHALTATLEEILEEERLIQGLMVETDD
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HREGVAAFKEKRKPVFQGK
CHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA