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The map label for this gene is pnp [H]

Identifier: 138894789

GI number: 138894789

Start: 1184963

End: 1187131

Strand: Direct

Name: pnp [H]

Synonym: GTNG_1123

Alternate gene names: 138894789

Gene position: 1184963-1187131 (Clockwise)

Preceding gene: 138894788

Following gene: 138894790

Centisome position: 33.38

GC content: 52.7

Gene sequence:

>2169_bases
ATGGAACAAGAGAAACGCGTGTTTTCCATCGATGTGGCCGGGCGTCCACTCGTCATTGAAACCGGCGAGTTGGCGAAGCA
GGCGAACGGCGCGGCGCTCGTCCGCTACGGTGATACCGTTGTGCTTAGCACGGCCACCGCATCGCGGGAAGCGAAAGACG
TTGACTTTTTCCCGCTGACCGTCAACTACGAGGAGCGGTTGTACGCCGTCGGGAAAATTCCAGGTGGATTTATTAAGCGC
GAGGGCCGTCCAAGCGAGAAAGCGATTTTGGCGAGCCGGCTCATCGACCGCCCGATTCGTCCGCTGTTTGCTGAAGGGTT
CCGCAATGAAGTGCAAGTCGTGTCCATGGTCATGAGCGTCGATCAAGACTGTTCCCCGGAAGTGGCAGCGTTAATCGGCT
CGTCGGTAGCGTTGACCATTTCTGACATTCCGTTTGAAGGGCCGATCGCTGGTGTCATTGTCGGTCGTATCGATGGCCAG
TTTGTCATCAACCCGACGGTTGAACAAATGGAAAAAAGCGATATGCATCTCATTGTGGCAGGTACGAAAGATGCCATCAA
CATGGTCGAGGCCGGCGCGGACGAAGTGCCGGAAGAAGTGATGCTTGAAGCCATTATGTTCGGCCATGAAGAAGTGAAAC
GGCTTATCGCTTTCCAAGAGGAAATCGCTGCTCAAGTTGGCAAAGAAAAGATGGAAGTGGTTCTATACGAGCCAGACCCG
GAACTAGAAGCGGAAATTCGCCAGCTTGCGGAAGCTGATATTAAGAAGGCCGTGCAAGTGCCGGAGAAACTGGCGCGCGA
TGCCGCCATTGAAGACGTAAAAGCTGGCGTTATTGCGAAATATGAAGCGGAAGAAGCTGATGAAGAGAAACTGAAGCAAG
TGCAAGAAATTTTGCATAAGCTTGTGAAAGAGGAAGTGCGCCGCTTAATTACGGTCGAGAAAATTCGTCCCGATGGGCGC
AAAGTTGATGAAATCCGCCCGCTGTCGTCAGCAGTTGGTATCTTGCCGCGCACGCATGGTTCCGGTCTGTTTACACGTGG
GCAAACACAAGTATTAAGCGTCTGCACGCTCGGAGCACTCGGTGATGTGCAAATTTTGGACGGGCTTGATCTTGAAGAAT
CGAAACGGTTTATGCACCATTACAATTTCCCGCCGTTTTCCGTTGGCGAAACGGGGCCGATGCGTGGGCCGGGGCGGCGT
GAAATTGGGCACGGGGCGCTAGGCGAGCGGGCGTTAGAGCCGGTCGTGCCGTCAGAGCGCGAGTTTCCGTACACGATCCG
CCTCGTTTCCGAAGTGCTGGAGTCGAACGGCTCGACGTCGCAAGCCAGCATTTGTGCGAGTACGCTGGCGATGATGGACG
CCGGGGTGCCAATTAAAGCGCCGGTTGCCGGCATTGCCATGGGGCTTGTGAAAAACGAGGACCATTATACGATTTTGACC
GATATTCAAGGCATTGAAGACCATCTTGGCGATATGGACTTTAAAGTCGCCGGCACGAGAAAAGGCGTCACGGCGCTGCA
AATGGACATTAAAATTAAAGGGCTGACGCGCGACATTTTGGAGGAGGCGCTACAGCAGGCACGCAAAGGTCGGCTCGAAA
TTTTAGACCATATGATGCAGACGCTCAGCGAGCCGCGTAAAGAGCTGTCTAAATATGCGCCGAAAATTTTGATCATGCAC
ATCAATCCGGATAAAATTCGCGAAGTCATCGGACCGAGCGGCAAACAAATCAACAAAATCATCGATGAAACCGGCGTGAA
AATCGACATCGAACAAGATGGCACGATTTTCATCTCGTCTGTCGATGAAGAAGCCAACCAAAAAGCGAAGCAAATTATCG
AAGATATCGTCCGTGAAGTCGAAGTTGGACAAGTGTATTTAGGCAAAGTGAAACGGATCGAAAAATTCGGCGCGTTCGTC
GAACTATTTAACGGCAAAGACGGGCTTGTCCACATTTCCGAACTTGCTGAAGAACGGATCGGCAAAGTGGAAGACATCGT
CTCGATCGGTGATGAAATTTTAGTGAAAGTGACGGAAATCGATAAGCAAGGACGTGTCAACTTGTCGCGCAAAGCGGTGT
TGCGCGAGCAGCGCGGTGCCGGAGAGTTGCCGAAAGAGACGCGGGAAAAACGAGGAAGACGGCCGGAGCGCCACCGCATG
AAGCCTTAG

Upstream 100 bases:

>100_bases
GGCGCAGTTTGCGCGGCAAATGATACAATGGCGCCAATTCGTCTATACTAATCATCAGCGATACCCATATTTTTATACAT
AGAGAGGAGTACTCGTTTGT

Downstream 100 bases:

>100_bases
GAATTTGTTTCTTAAGGCTCTTTTGTTATGTTCTAACCTGTCCCTCTGCTACATAATTTGTAGTAGTAAAAAAGAGGGGG
GACAAGGGTGAACAACGGTT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MEQEKRVFSIDVAGRPLVIETGELAKQANGAALVRYGDTVVLSTATASREAKDVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFAEGFRNEVQVVSMVMSVDQDCSPEVAALIGSSVALTISDIPFEGPIAGVIVGRIDGQ
FVINPTVEQMEKSDMHLIVAGTKDAINMVEAGADEVPEEVMLEAIMFGHEEVKRLIAFQEEIAAQVGKEKMEVVLYEPDP
ELEAEIRQLAEADIKKAVQVPEKLARDAAIEDVKAGVIAKYEAEEADEEKLKQVQEILHKLVKEEVRRLITVEKIRPDGR
KVDEIRPLSSAVGILPRTHGSGLFTRGQTQVLSVCTLGALGDVQILDGLDLEESKRFMHHYNFPPFSVGETGPMRGPGRR
EIGHGALGERALEPVVPSEREFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKNEDHYTILT
DIQGIEDHLGDMDFKVAGTRKGVTALQMDIKIKGLTRDILEEALQQARKGRLEILDHMMQTLSEPRKELSKYAPKILIMH
INPDKIREVIGPSGKQINKIIDETGVKIDIEQDGTIFISSVDEEANQKAKQIIEDIVREVEVGQVYLGKVKRIEKFGAFV
ELFNGKDGLVHISELAEERIGKVEDIVSIGDEILVKVTEIDKQGRVNLSRKAVLREQRGAGELPKETREKRGRRPERHRM
KP

Sequences:

>Translated_722_residues
MEQEKRVFSIDVAGRPLVIETGELAKQANGAALVRYGDTVVLSTATASREAKDVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFAEGFRNEVQVVSMVMSVDQDCSPEVAALIGSSVALTISDIPFEGPIAGVIVGRIDGQ
FVINPTVEQMEKSDMHLIVAGTKDAINMVEAGADEVPEEVMLEAIMFGHEEVKRLIAFQEEIAAQVGKEKMEVVLYEPDP
ELEAEIRQLAEADIKKAVQVPEKLARDAAIEDVKAGVIAKYEAEEADEEKLKQVQEILHKLVKEEVRRLITVEKIRPDGR
KVDEIRPLSSAVGILPRTHGSGLFTRGQTQVLSVCTLGALGDVQILDGLDLEESKRFMHHYNFPPFSVGETGPMRGPGRR
EIGHGALGERALEPVVPSEREFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKNEDHYTILT
DIQGIEDHLGDMDFKVAGTRKGVTALQMDIKIKGLTRDILEEALQQARKGRLEILDHMMQTLSEPRKELSKYAPKILIMH
INPDKIREVIGPSGKQINKIIDETGVKIDIEQDGTIFISSVDEEANQKAKQIIEDIVREVEVGQVYLGKVKRIEKFGAFV
ELFNGKDGLVHISELAEERIGKVEDIVSIGDEILVKVTEIDKQGRVNLSRKAVLREQRGAGELPKETREKRGRRPERHRM
KP
>Mature_722_residues
MEQEKRVFSIDVAGRPLVIETGELAKQANGAALVRYGDTVVLSTATASREAKDVDFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILASRLIDRPIRPLFAEGFRNEVQVVSMVMSVDQDCSPEVAALIGSSVALTISDIPFEGPIAGVIVGRIDGQ
FVINPTVEQMEKSDMHLIVAGTKDAINMVEAGADEVPEEVMLEAIMFGHEEVKRLIAFQEEIAAQVGKEKMEVVLYEPDP
ELEAEIRQLAEADIKKAVQVPEKLARDAAIEDVKAGVIAKYEAEEADEEKLKQVQEILHKLVKEEVRRLITVEKIRPDGR
KVDEIRPLSSAVGILPRTHGSGLFTRGQTQVLSVCTLGALGDVQILDGLDLEESKRFMHHYNFPPFSVGETGPMRGPGRR
EIGHGALGERALEPVVPSEREFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKNEDHYTILT
DIQGIEDHLGDMDFKVAGTRKGVTALQMDIKIKGLTRDILEEALQQARKGRLEILDHMMQTLSEPRKELSKYAPKILIMH
INPDKIREVIGPSGKQINKIIDETGVKIDIEQDGTIFISSVDEEANQKAKQIIEDIVREVEVGQVYLGKVKRIEKFGAFV
ELFNGKDGLVHISELAEERIGKVEDIVSIGDEILVKVTEIDKQGRVNLSRKAVLREQRGAGELPKETREKRGRRPERHRM
KP

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=38.1284916201117, Blast_Score=458, Evalue=1e-128,
Organism=Escherichia coli, GI145693187, Length=681, Percent_Identity=50.9544787077827, Blast_Score=672, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=652, Percent_Identity=34.9693251533742, Blast_Score=353, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6320850, Length=102, Percent_Identity=39.2156862745098, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=39.3767705382436, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=39.3767705382436, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=39.3767705382436, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=39.117199391172, Blast_Score=433, Evalue=1e-121,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 79798; Mature: 79798

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQEKRVFSIDVAGRPLVIETGELAKQANGAALVRYGDTVVLSTATASREAKDVDFFPLT
CCCCCEEEEEEECCCCEEEECHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCEEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFAEGFRNEVQVVSMVMSV
ECHHHHEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
DQDCSPEVAALIGSSVALTISDIPFEGPIAGVIVGRIDGQFVINPTVEQMEKSDMHLIVA
CCCCCHHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCEEEECCCHHHHCCCCCEEEEE
GTKDAINMVEAGADEVPEEVMLEAIMFGHEEVKRLIAFQEEIAAQVGKEKMEVVLYEPDP
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
ELEAEIRQLAEADIKKAVQVPEKLARDAAIEDVKAGVIAKYEAEEADEEKLKQVQEILHK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
LVKEEVRRLITVEKIRPDGRKVDEIRPLSSAVGILPRTHGSGLFTRGQTQVLSVCTLGAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHCCC
GDVQILDGLDLEESKRFMHHYNFPPFSVGETGPMRGPGRREIGHGALGERALEPVVPSER
CCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCC
EFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKNEDHYTILT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEEEE
DIQGIEDHLGDMDFKVAGTRKGVTALQMDIKIKGLTRDILEEALQQARKGRLEILDHMMQ
CCCCHHHHHCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHH
TLSEPRKELSKYAPKILIMHINPDKIREVIGPSGKQINKIIDETGVKIDIEQDGTIFISS
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEEEE
VDEEANQKAKQIIEDIVREVEVGQVYLGKVKRIEKFGAFVELFNGKDGLVHISELAEERI
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
GKVEDIVSIGDEILVKVTEIDKQGRVNLSRKAVLREQRGAGELPKETREKRGRRPERHRM
CHHHHHHHCCHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHCCC
KP
CC
>Mature Secondary Structure
MEQEKRVFSIDVAGRPLVIETGELAKQANGAALVRYGDTVVLSTATASREAKDVDFFPLT
CCCCCEEEEEEECCCCEEEECHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCEEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFAEGFRNEVQVVSMVMSV
ECHHHHEEEEECCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
DQDCSPEVAALIGSSVALTISDIPFEGPIAGVIVGRIDGQFVINPTVEQMEKSDMHLIVA
CCCCCHHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCEEEECCCHHHHCCCCCEEEEE
GTKDAINMVEAGADEVPEEVMLEAIMFGHEEVKRLIAFQEEIAAQVGKEKMEVVLYEPDP
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
ELEAEIRQLAEADIKKAVQVPEKLARDAAIEDVKAGVIAKYEAEEADEEKLKQVQEILHK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
LVKEEVRRLITVEKIRPDGRKVDEIRPLSSAVGILPRTHGSGLFTRGQTQVLSVCTLGAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHCCC
GDVQILDGLDLEESKRFMHHYNFPPFSVGETGPMRGPGRREIGHGALGERALEPVVPSER
CCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCC
EFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKNEDHYTILT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEEEE
DIQGIEDHLGDMDFKVAGTRKGVTALQMDIKIKGLTRDILEEALQQARKGRLEILDHMMQ
CCCCHHHHHCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHH
TLSEPRKELSKYAPKILIMHINPDKIREVIGPSGKQINKIIDETGVKIDIEQDGTIFISS
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEEEE
VDEEANQKAKQIIEDIVREVEVGQVYLGKVKRIEKFGAFVELFNGKDGLVHISELAEERI
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
GKVEDIVSIGDEILVKVTEIDKQGRVNLSRKAVLREQRGAGELPKETREKRGRRPERHRM
CHHHHHHHCCHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHCCC
KP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA