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The map label for this gene is queE [H]

Identifier: 138894535

GI number: 138894535

Start: 941564

End: 942241

Strand: Direct

Name: queE [H]

Synonym: GTNG_0865

Alternate gene names: 138894535

Gene position: 941564-942241 (Clockwise)

Preceding gene: 138894534

Following gene: 138894536

Centisome position: 26.52

GC content: 56.05

Gene sequence:

>678_bases
ATGGTAATCGGCCAAAAGACGATGTTCGTCCGCACCGCCGGCTGCGATTACCGCTGCCGTTGGTGTGATTCAGCCTTCAC
GTGGGACGGGTCGGCGAAAGAAGAGATCGAACAGCTGACGGCAGAAGATATTTGGCAGCGGCTTGAAGCCATTGGCGGTC
GTCGCTTCCGCCATGTGACGATTTCAGGCGGCAACCCGCTTCTGCTCGCCGCTCTTGGTGAGCTCGTTGCGCTCCTTCAT
GAAAAAGGGGTACAAGTGGCGGTCGAGACGCAAGGGAGCCGCTGGCAAGATTGGTTGCTTGACGTTGACGATGTCACCAT
TTCCCCGAAGCCGCCAAGTTCGGGGATGGATACCGACTGGGCGATGCTCGATTCCATCATCAGGAAGCTGCTGGCTGATC
AAAATCGGACGCGGCGTGTTAGCCTGAAAGTTGTCGTCTTCGATGAGGCGGATTTGGCGTATGCGAAAGAAGTGCATCGC
CGTTACCCGGGAGTTCCATTTTATTTGCAGACCGGCAACGCCGATGTCGAGGCGCTCGATACGAATGCGCTTCGCATGAA
GCTCTTTGACCAGCTTGATTGGCTAGTGGAACGGGTGGCTCAATCAGATGAGCTGAATGACGTTCATGTGTTGCCGCAAC
TGCACACGCTTCTTTGGGGAACGAAGCGCGGTGTCTAA

Upstream 100 bases:

>100_bases
CGACCAGTTATGTTATTTACCGCCCGAAAGTGGGGGAACGGTAGTGGCTCGTACGATTCCAGTATTAGAAATTTTCGGGC
CGACGATCCAAGGAGAAGGG

Downstream 100 bases:

>100_bases
AGCTGCACGCTGGTCCATCACGAAATGATAAAGAAGGAGGAATGACCAATGAATGGAAGGAAAGATGAAGAATTAAAAGA
CTTGACGCTGCTTGGCAATC

Product: organic radical activating protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MVIGQKTMFVRTAGCDYRCRWCDSAFTWDGSAKEEIEQLTAEDIWQRLEAIGGRRFRHVTISGGNPLLLAALGELVALLH
EKGVQVAVETQGSRWQDWLLDVDDVTISPKPPSSGMDTDWAMLDSIIRKLLADQNRTRRVSLKVVVFDEADLAYAKEVHR
RYPGVPFYLQTGNADVEALDTNALRMKLFDQLDWLVERVAQSDELNDVHVLPQLHTLLWGTKRGV

Sequences:

>Translated_225_residues
MVIGQKTMFVRTAGCDYRCRWCDSAFTWDGSAKEEIEQLTAEDIWQRLEAIGGRRFRHVTISGGNPLLLAALGELVALLH
EKGVQVAVETQGSRWQDWLLDVDDVTISPKPPSSGMDTDWAMLDSIIRKLLADQNRTRRVSLKVVVFDEADLAYAKEVHR
RYPGVPFYLQTGNADVEALDTNALRMKLFDQLDWLVERVAQSDELNDVHVLPQLHTLLWGTKRGV
>Mature_225_residues
MVIGQKTMFVRTAGCDYRCRWCDSAFTWDGSAKEEIEQLTAEDIWQRLEAIGGRRFRHVTISGGNPLLLAALGELVALLH
EKGVQVAVETQGSRWQDWLLDVDDVTISPKPPSSGMDTDWAMLDSIIRKLLADQNRTRRVSLKVVVFDEADLAYAKEVHR
RYPGVPFYLQTGNADVEALDTNALRMKLFDQLDWLVERVAQSDELNDVHVLPQLHTLLWGTKRGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 25506; Mature: 25506

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIGQKTMFVRTAGCDYRCRWCDSAFTWDGSAKEEIEQLTAEDIWQRLEAIGGRRFRHVT
CEECCEEEEEEECCCCCEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
ISGGNPLLLAALGELVALLHEKGVQVAVETQGSRWQDWLLDVDDVTISPKPPSSGMDTDW
ECCCCEEHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHEEECCCEEECCCCCCCCCCCHH
AMLDSIIRKLLADQNRTRRVSLKVVVFDEADLAYAKEVHRRYPGVPFYLQTGNADVEALD
HHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEC
TNALRMKLFDQLDWLVERVAQSDELNDVHVLPQLHTLLWGTKRGV
CHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVIGQKTMFVRTAGCDYRCRWCDSAFTWDGSAKEEIEQLTAEDIWQRLEAIGGRRFRHVT
CEECCEEEEEEECCCCCEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
ISGGNPLLLAALGELVALLHEKGVQVAVETQGSRWQDWLLDVDDVTISPKPPSSGMDTDW
ECCCCEEHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHEEECCCEEECCCCCCCCCCCHH
AMLDSIIRKLLADQNRTRRVSLKVVVFDEADLAYAKEVHRRYPGVPFYLQTGNADVEALD
HHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEC
TNALRMKLFDQLDWLVERVAQSDELNDVHVLPQLHTLLWGTKRGV
CHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]