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The map label for this gene is queF [H]

Identifier: 138894536

GI number: 138894536

Start: 942290

End: 942787

Strand: Direct

Name: queF [H]

Synonym: GTNG_0866

Alternate gene names: 138894536

Gene position: 942290-942787 (Clockwise)

Preceding gene: 138894535

Following gene: 138894537

Centisome position: 26.54

GC content: 44.38

Gene sequence:

>498_bases
ATGAATGGAAGGAAAGATGAAGAATTAAAAGACTTGACGCTGCTTGGCAATCAAGGGACAACGTATTCGTTTACGTACGA
TCCCAATCTTCTTGAAGTGTTTGACAACAAACATCCTGACCGGGATTATTTCGTGAAGTTCAATTGTCCGGAGTTTACGA
GTCTATGTCCAAAAACTCGCCAACCGGACTTTGCGACGATTTACATTAGTTATATTCCGGACAAAAAATGCGTCGAAAGC
AAGTCGCTGAAACTTTATTTGTTTAGTTTCCGCAACCATGGCGATTTTCACGAAGACTGTGTCAACATCATTATGAACGA
TTTAATCAACGTGATGGAACCGCGCTACATTGAAGTATGGGGCAAATTCACCCCGCGCGGCGGCATTTCGATCGACCCGT
ATTGCAACTGGGGACGCCCGGGGACGAAATACGAAAAAATGGCCGAGTATCGGCTGCTCAACCATGACCTATACCCAGAA
AAAATCGATAACCGCTAA

Upstream 100 bases:

>100_bases
TGTGTTGCCGCAACTGCACACGCTTCTTTGGGGAACGAAGCGCGGTGTCTAAAGCTGCACGCTGGTCCATCACGAAATGA
TAAAGAAGGAGGAATGACCA

Downstream 100 bases:

>100_bases
AGCCGGCAAAAAATGAGGTGGTTTTTTCGCCAAAACTCATATATAATGGACAATGAATTTTTGCTTGGAGGTTTTGGCGT
TGGAGCGAAGAACAGCTCTT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MNGRKDEELKDLTLLGNQGTTYSFTYDPNLLEVFDNKHPDRDYFVKFNCPEFTSLCPKTRQPDFATIYISYIPDKKCVES
KSLKLYLFSFRNHGDFHEDCVNIIMNDLINVMEPRYIEVWGKFTPRGGISIDPYCNWGRPGTKYEKMAEYRLLNHDLYPE
KIDNR

Sequences:

>Translated_165_residues
MNGRKDEELKDLTLLGNQGTTYSFTYDPNLLEVFDNKHPDRDYFVKFNCPEFTSLCPKTRQPDFATIYISYIPDKKCVES
KSLKLYLFSFRNHGDFHEDCVNIIMNDLINVMEPRYIEVWGKFTPRGGISIDPYCNWGRPGTKYEKMAEYRLLNHDLYPE
KIDNR
>Mature_165_residues
MNGRKDEELKDLTLLGNQGTTYSFTYDPNLLEVFDNKHPDRDYFVKFNCPEFTSLCPKTRQPDFATIYISYIPDKKCVES
KSLKLYLFSFRNHGDFHEDCVNIIMNDLINVMEPRYIEVWGKFTPRGGISIDPYCNWGRPGTKYEKMAEYRLLNHDLYPE
KIDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=113, Percent_Identity=33.6283185840708, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 19529; Mature: 19529

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGRKDEELKDLTLLGNQGTTYSFTYDPNLLEVFDNKHPDRDYFVKFNCPEFTSLCPKTR
CCCCCCCCCCEEEEECCCCCEEEEEECCCHHEECCCCCCCCCEEEEECCCCHHHHCCCCC
QPDFATIYISYIPDKKCVESKSLKLYLFSFRNHGDFHEDCVNIIMNDLINVMEPRYIEVW
CCCEEEEEEEECCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
GKFTPRGGISIDPYCNWGRPGTKYEKMAEYRLLNHDLYPEKIDNR
ECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MNGRKDEELKDLTLLGNQGTTYSFTYDPNLLEVFDNKHPDRDYFVKFNCPEFTSLCPKTR
CCCCCCCCCCEEEEECCCCCEEEEEECCCHHEECCCCCCCCCEEEEECCCCHHHHCCCCC
QPDFATIYISYIPDKKCVESKSLKLYLFSFRNHGDFHEDCVNIIMNDLINVMEPRYIEVW
CCCEEEEEEEECCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
GKFTPRGGISIDPYCNWGRPGTKYEKMAEYRLLNHDLYPEKIDNR
ECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA