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The map label for this gene is gltX [H]

Identifier: 138893762

GI number: 138893762

Start: 101498

End: 102967

Strand: Direct

Name: gltX [H]

Synonym: GTNG_0083

Alternate gene names: 138893762

Gene position: 101498-102967 (Clockwise)

Preceding gene: 138893761

Following gene: 138893763

Centisome position: 2.86

GC content: 50.34

Gene sequence:

>1470_bases
ATGGCAAAAAACGTGCGCGTGCGCTATGCGCCGAGCCCGACTGGCCATTTGCATATCGGTGGGGCACGGACAGCGCTGTT
TAACTATTTGTTTGCCCGCCATTACGGCGGAAAAATGGTCGTCCGCATCGAAGATACGGATATTGAACGGAACGTTGAAG
GCGGCGAAGAGTCGCAGCTTGAAAACTTAAAATGGCTTGGCATCGATTATGACGAATCGATTGATAAGGACGGCGGATAT
GGGCCGTATCGTCAGACGGAACGGCTCGATATCTATCGGAAGTATGTGAACGAGCTGCTTGAACAAGGGCATGCGTATAA
ATGTTTTTGTACACCGGAAGAGCTCGAGCGGGAACGTGAGGAGCAACGGGCGGCAGGTATTGCTGCTCCGCAATACAGCG
GCAAATGCCGCCATTTAACGCCGGAGCAAGTTGCCGAGCTTGAAGCACAAGGAAAACCGTATACGATCCGCTTGAAAGTG
CCGGAAGGGAAAACGTATGAAGTAGATGATTTAGTGCGCGGTAAAGTGACGTTTGAATCGAAAGACATCGGCGATTGGGT
CATTGTGAAGGCGAACGGTATTCCGACGTACAACTTTGCCGTTGTCATTGATGACCATTTGATGGAAATCAGCCATGTGT
TCCGCGGTGAGGAGCATTTATCCAACACGCCGAAACAGCTAATGGTGTACGAATATTTCGGTTGGGAGCCACCGCAATTC
GCCCATATGACATTGATTGTCAACGAGCAGCGGAAAAAGCTATCCAAGCGCGATGAATCGATTATTCAGTTCGTGTCGCA
ATATAAAGAGCTCGGCTATTTGCCGGAGGCGATGTTCAACTTTTTCGCCCTTCTTGGCTGGTCGCCGGAAGGAGAAGAAG
AAATTTTTACGAAGGACGAGCTCATCCGCATTTTTGATGTCGCCCGGCTGTCGAAATCGCCGTCGATGTTTGATACGAAA
AAGCTGACATGGATGAACAACCAATATATCAAAAAGCTGGATCTCGACAGGCTTGTCGAGCTGGCGTTGCCGCATTTAGT
GAAAGCCGGACGCCTGCCGGCAGATATGAGTGATGAGCAGCGGCAATGGGCACGCGATTTAATTGCCTTGTACCAAGAGC
AAATGAGCTACGGTGCGGAGATCGTTTCGCTGTCCGAGCTGTTCTTTAAAGAAGAAGTCGAATACGAAGACGAAGCCCGC
CAAGTGCTCGCCGAAGAACAAGTACCGGATGTGCTCTCCGCCTTTTTGGCGAATGTGCGTGAGCTTGAGCCGTTTACGGC
GGATGAGATTAAAGCAGCGATCAAAGCAGTGCAAAAATCGACAGGGCAAAAAGGCAAGAAGCTGTTTATGCCGATTCGCG
CCGCAGTGACTGGGCAAACACACGGACCGGAACTGCCGTTTGCCATCCAACTGCTTGGCAAACAAAAGGTGATTGAACGG
CTCGAACGGGCACTGCATGAAAAATTTTAA

Upstream 100 bases:

>100_bases
AGCGGCATGGTGCCGCTAAGGTGAGCCTTCCGTTTTGAACTTTGCCACGATAAACGGTACAATAATAGCACAAGCTTTGA
TGGAATTGGAGGTTTGGACG

Downstream 100 bases:

>100_bases
TCAGTCTTTTATGCACGTTTGTTTTGTAGCTGTGGTATAATAATAAAAAGTTTTTGGCTGAACAAGTACACATCATGACA
AAGCGTTGACGAGGAGAAGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MAKNVRVRYAPSPTGHLHIGGARTALFNYLFARHYGGKMVVRIEDTDIERNVEGGEESQLENLKWLGIDYDESIDKDGGY
GPYRQTERLDIYRKYVNELLEQGHAYKCFCTPEELEREREEQRAAGIAAPQYSGKCRHLTPEQVAELEAQGKPYTIRLKV
PEGKTYEVDDLVRGKVTFESKDIGDWVIVKANGIPTYNFAVVIDDHLMEISHVFRGEEHLSNTPKQLMVYEYFGWEPPQF
AHMTLIVNEQRKKLSKRDESIIQFVSQYKELGYLPEAMFNFFALLGWSPEGEEEIFTKDELIRIFDVARLSKSPSMFDTK
KLTWMNNQYIKKLDLDRLVELALPHLVKAGRLPADMSDEQRQWARDLIALYQEQMSYGAEIVSLSELFFKEEVEYEDEAR
QVLAEEQVPDVLSAFLANVRELEPFTADEIKAAIKAVQKSTGQKGKKLFMPIRAAVTGQTHGPELPFAIQLLGKQKVIER
LERALHEKF

Sequences:

>Translated_489_residues
MAKNVRVRYAPSPTGHLHIGGARTALFNYLFARHYGGKMVVRIEDTDIERNVEGGEESQLENLKWLGIDYDESIDKDGGY
GPYRQTERLDIYRKYVNELLEQGHAYKCFCTPEELEREREEQRAAGIAAPQYSGKCRHLTPEQVAELEAQGKPYTIRLKV
PEGKTYEVDDLVRGKVTFESKDIGDWVIVKANGIPTYNFAVVIDDHLMEISHVFRGEEHLSNTPKQLMVYEYFGWEPPQF
AHMTLIVNEQRKKLSKRDESIIQFVSQYKELGYLPEAMFNFFALLGWSPEGEEEIFTKDELIRIFDVARLSKSPSMFDTK
KLTWMNNQYIKKLDLDRLVELALPHLVKAGRLPADMSDEQRQWARDLIALYQEQMSYGAEIVSLSELFFKEEVEYEDEAR
QVLAEEQVPDVLSAFLANVRELEPFTADEIKAAIKAVQKSTGQKGKKLFMPIRAAVTGQTHGPELPFAIQLLGKQKVIER
LERALHEKF
>Mature_488_residues
AKNVRVRYAPSPTGHLHIGGARTALFNYLFARHYGGKMVVRIEDTDIERNVEGGEESQLENLKWLGIDYDESIDKDGGYG
PYRQTERLDIYRKYVNELLEQGHAYKCFCTPEELEREREEQRAAGIAAPQYSGKCRHLTPEQVAELEAQGKPYTIRLKVP
EGKTYEVDDLVRGKVTFESKDIGDWVIVKANGIPTYNFAVVIDDHLMEISHVFRGEEHLSNTPKQLMVYEYFGWEPPQFA
HMTLIVNEQRKKLSKRDESIIQFVSQYKELGYLPEAMFNFFALLGWSPEGEEEIFTKDELIRIFDVARLSKSPSMFDTKK
LTWMNNQYIKKLDLDRLVELALPHLVKAGRLPADMSDEQRQWARDLIALYQEQMSYGAEIVSLSELFFKEEVEYEDEARQ
VLAEEQVPDVLSAFLANVRELEPFTADEIKAAIKAVQKSTGQKGKKLFMPIRAAVTGQTHGPELPFAIQLLGKQKVIERL
ERALHEKF

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=498, Percent_Identity=33.9357429718875, Blast_Score=258, Evalue=7e-69,
Organism=Escherichia coli, GI1788743, Length=485, Percent_Identity=37.7319587628866, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI226510925, Length=256, Percent_Identity=34.375, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=34.639175257732, Blast_Score=271, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6324540, Length=507, Percent_Identity=35.1084812623274, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321192, Length=224, Percent_Identity=29.4642857142857, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=308, Percent_Identity=23.7012987012987, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24665321, Length=502, Percent_Identity=36.6533864541833, Blast_Score=302, Evalue=4e-82,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56325; Mature: 56194

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNVRVRYAPSPTGHLHIGGARTALFNYLFARHYGGKMVVRIEDTDIERNVEGGEESQL
CCCCCEEEECCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHH
ENLKWLGIDYDESIDKDGGYGPYRQTERLDIYRKYVNELLEQGHAYKCFCTPEELERERE
CCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
EQRAAGIAAPQYSGKCRHLTPEQVAELEAQGKPYTIRLKVPEGKTYEVDDLVRGKVTFES
HHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHCCEEEECC
KDIGDWVIVKANGIPTYNFAVVIDDHLMEISHVFRGEEHLSNTPKQLMVYEYFGWEPPQF
CCCCCEEEEEECCCCCEEEEEEECHHHHHHHHHHCCHHHHCCCCHHEEEEEECCCCCCCC
AHMTLIVNEQRKKLSKRDESIIQFVSQYKELGYLPEAMFNFFALLGWSPEGEEEIFTKDE
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LIRIFDVARLSKSPSMFDTKKLTWMNNQYIKKLDLDRLVELALPHLVKAGRLPADMSDEQ
HHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHH
RQWARDLIALYQEQMSYGAEIVSLSELFFKEEVEYEDEARQVLAEEQVPDVLSAFLANVR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHH
ELEPFTADEIKAAIKAVQKSTGQKGKKLFMPIRAAVTGQTHGPELPFAIQLLGKQKVIER
HCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
LERALHEKF
HHHHHHHCC
>Mature Secondary Structure 
AKNVRVRYAPSPTGHLHIGGARTALFNYLFARHYGGKMVVRIEDTDIERNVEGGEESQL
CCCCEEEECCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHH
ENLKWLGIDYDESIDKDGGYGPYRQTERLDIYRKYVNELLEQGHAYKCFCTPEELERERE
CCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
EQRAAGIAAPQYSGKCRHLTPEQVAELEAQGKPYTIRLKVPEGKTYEVDDLVRGKVTFES
HHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHCCEEEECC
KDIGDWVIVKANGIPTYNFAVVIDDHLMEISHVFRGEEHLSNTPKQLMVYEYFGWEPPQF
CCCCCEEEEEECCCCCEEEEEEECHHHHHHHHHHCCHHHHCCCCHHEEEEEECCCCCCCC
AHMTLIVNEQRKKLSKRDESIIQFVSQYKELGYLPEAMFNFFALLGWSPEGEEEIFTKDE
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LIRIFDVARLSKSPSMFDTKKLTWMNNQYIKKLDLDRLVELALPHLVKAGRLPADMSDEQ
HHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHH
RQWARDLIALYQEQMSYGAEIVSLSELFFKEEVEYEDEARQVLAEEQVPDVLSAFLANVR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHH
ELEPFTADEIKAAIKAVQKSTGQKGKKLFMPIRAAVTGQTHGPELPFAIQLLGKQKVIER
HCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
LERALHEKF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA